• In order to support the comprehensive classification of Leukocyte Adhesion Deficiency-I (LAD-I) severity by simultaneous screening of CD11a/CD18, this study assessed clinical, laboratory, and genetic findings along with outcomes of 69 LAD-I patients during the last 15 years. (nih.gov)
  • The C71/16 monoclonal antibody specifically binds to mouse CD18 which is also known as the common β2 chain of LFA-1 (CD11a/CD18, αLβ2 integrin), Mac-1 (CD11b/CD18, αMβ2 integrin), and gp150, 95 (CD11c/CD18, αxβ2 integrin). (bdbiosciences.com)
  • Expression of CD18 is limited to leukocytes, where it is widely distributed in consort with the three integrin α chains (CD11a, CD11b, and CD11c). (bdbiosciences.com)
  • CD18 non-covalently associates with CD11a, CD11b, or CD11c. (biolegend.com)
  • Background: CD18, integrin beta2 subunit, forms heterodimers with four types of CD11 molecule to constitute leukocyte (beta2) integrins: alphaLbeta2 (CD11a/CD18, LFA-1), alphaMbeta2 (CD11b/CD18, Mac-1, CR3), alphaXbeta2 (CD11c/CD18) and alphaDbeta2 (CD11d/CD18). (icbinhibitor.com)
  • CD11a/CD18 (LFA-1) expressed on lymphocytes is known to play an important role in lymphocyte trafficking (adhesion to vascular endothelium), as well as interactions to antigen presenting cells (APC). (medscape.com)
  • Three CD11 alpha chains and a common CD18 beta chain form heterodimer transmembrane complexes (CD11a/CD18, CD11b/CD18, CD11c/CD18). (medscape.com)
  • CD11a/CD18 is also known as leukocyte factor antigen-1 (LFA-1), CD11b/CD18 is known as CR3, and CD11c/CD18 is known as CR4. (medscape.com)
  • Ligands for CD11a/CD18 are intercellular adhesion molecules (ICAMs), ligands for CD11b/CD18 are complement C3bi and ICAMs, and ligands for CD11c/CD18 are C3bi and ICAMs. (medscape.com)
  • It is a type I transmembrane glycoprotein with two Ig-like C2-type domains, and binds to the leukocyte integrins LFA-1 (CD11a/CD18) and Mac-1 (CD11b/CD18). (betalifesci.com)
  • With this, we analyzed the phenotypes of CD34 + HSCs and showed that primitive HSCs or CD34 + CD33 - cells expressed adhesion molecules such as CD43, CD44, CD11a, CD11c, CD18, and leukocyte adhesion molecule (LAM-1). (elsevierpure.com)
  • When CD18 or CD11a antibody was added to a coculture system of CD34 + CD33 - cells with stromal layers, the number of generated CFCs decreased significantly compared with the no antibody control. (elsevierpure.com)
  • Leukocyte function-associated antigen-1 (LFA-1) (CD11a/CD18) was expressed on some populations of hematopoietic cells and contributed to the proliferation by interacting with stromal cells. (elsevierpure.com)
  • Leukocyte function-associated antigen-1 ( LFA-1 ), also known as CD11a/CD18 and αLβ2, is a member of the β2-integrin family found on lymphocytes and other leukocytes. (immune-system-research.com)
  • In humans, lymphocyte adhesion to cells is mediated by the protein heterodimer CD11a/CD18 (Leu-CAMa, LFA-1) and its ligand CD54 (ICAM-1). (csb-bioenergetics.nl)
  • ICAM-1 binds to integrins of type CD11a/CD18 (leukocyte adhesion molecule, LFA-1), or CD11b/CD18 (Mac-1) and is exploited by Rhinovirus as a receptor. (thermofisher.com)
  • The beta-2 (B2) integrin (leukocyte integrin, CD11/CD18) subfamily comprising three members, each consisting of a shared beta subunit (CD18) non-covalently associated with unique alpha subunits (CD11a, CD11b, CD11c). (medicaljournals.se)
  • CD11a is a leukocyte marker that is expressed in B and T lymphocytes, macrophages, monocytes, neutrophils, basophils and eosinophils. (thermofisher.com)
  • ICAM-3 is the major ligand for LFA-1 (CD11a/CD18) in the resting state. (ximbio.com)
  • The dissociation rates were calculated under low force conditions for ICAM-1 interactions with both β 2 -integrins, LFA-1 (CD11a/CD18) and Mac-1 (CD11b/CD18), together and separately by using functional blocking antibodies on PMNs. (psu.edu)
  • CD18, also known as integrin β2 subunit, LFA-1 β subunit, and β2 integrin, is a 90 - 95 kD type I glycoprotein. (biolegend.com)
  • The CD11/CD18 complex is part of the beta-2 integrin family and is important in adhesion and phagocytosis (see Table 1). (medscape.com)
  • Leukocyte adhesion deficiency-III is caused by mutations in KINDLIN3 affecting integrin activation. (ox.ac.uk)
  • Beta1 and beta2 integrin function on leukocytes is crucial for a successful immune response and the platelet integrin alpha(IIb)beta3 initiates the process of blood clotting through binding fibrinogen. (ox.ac.uk)
  • In addition, integrin αs are active signaling receptors which recruit leukocytes to inflammatory sites and promote cell activation. (cytekbio.com)
  • integrin αalpha M (CD11b) assembles with integrin αbeta-2 (CD18) into a receptor known as Macrophage Antigen-1 (Mac-1) or complement receptor type 3 (CR3). (cytekbio.com)
  • ICAM-1 is an integral membrane protein, a member of the immunoglobulin superfamily, and a ligand for LFA-1, a beta 2 leukocyte integrin. (csb-bioenergetics.nl)
  • CD11b non-covalently associates with CD18 (β2 integrin) to form Mac-1. (biolegend.com)
  • Description: The N418 monoclonal antibody reacts with mouse CD11c, the integrin alpha X. CD11c non-covalently associates with beta 2 integrin to form the CD11c/CD18 heterodimer. (thermofisher.com)
  • In vitro studies of phagocytic cells from an infant with leukocyte adhesion deficiency type I demonstrated that complement receptor 3 (CD18/CD11b) mediates nonopsonic phagocytosis of some Pseudomonas aeruginosa strains and might play a control role in the control of Pseudomonas infections at sites where there are low levels of opsonins. (ox.ac.uk)
  • CD18 deficiency results in loss of expression of LFA-1, CR3 (CD11b/CD18), and CR4 (CD11c/CD18) (see Table 3). (medscape.com)
  • Physicians' awareness of LAD-I considering delayed separation of umbilical cord marked neutrophilic leukocytosis, and variability in CD11 and CD18 expression levels, and genetic analysis leads to early diagnosis and defining disease severity. (nih.gov)
  • They are known to bind to leucocyte integrins CD11/CD18 such as LFA-1 and Macrophage-1 antigen, during inflammation and in immune responses. (wikipedia.org)
  • Children with leukocyte adhesion deficiency type I are at risk for overwhelming infection because their neutrophils lack surface beta 2 integrins (CD18/CD11) that normally interact with endothelial cell adhesion molecules and mediate migration to sites of bacterial invasion. (ox.ac.uk)
  • CD11, along with CD18, form a heterodimer adhesion molecule. (thermofisher.com)
  • This increase in neutrophil adhesion caused by CD66 antibodies was blocked by a CD18 antibody and associated with up-regalation of CD11/CD18 on the neutrophil surface. (umn.edu)
  • The data suggest that CD66, CD66b, CD66c, and CD66d antibody binding to the neutrophil surface triggers a transient activation signal that requires extracellular calcium and regulates the adhesive activity of CD11/CD18. (umn.edu)
  • antibody responses to polysaccharide antigens are decreased and antibody responses to protein antigens are slightly reduced. (medscape.com)
  • Patients with leukocyte adhesion deficiency II manifest the Bombay phenotype (ie, negative for O and H blood group antigens with potential production of anti-H antibody). (medscape.com)
  • The Langerhans' cells (LC) were identified with a monoclonal antibody to OKT6 antigen, by employing an immunofluorescence technique. (medicaljournals.se)
  • The β2 integrins are important mediators of leukocyte-endothelium interactions. (bdbiosciences.com)
  • These integrins are essential for proper leukocyte migration, mediating intercellular contacts. (icbinhibitor.com)
  • Leukocyte adhesion deficiency type I (LAD I) is a failure to express CD18, which composes the common ß 2 subunit of LFA1 family (ß2 integrins). (medscape.com)
  • Integrins are the major adhesion receptors of leukocytes and platelets. (ox.ac.uk)
  • Subjects with leukocyte adhesion deficiency-1 (LAD-I) do not express beta2 integrins because of mutations in the gene specifying the beta2 subunit, and they suffer recurrent bacterial infections. (ox.ac.uk)
  • La subunidad alfa es el ANTÍGENO CD11c, un antígeno de superficie que se expresa en algunas células mieloides. (bvsalud.org)
  • The alpha subunit is the CD11C ANTIGEN, a surface antigen expressed on some myeloid cells. (bvsalud.org)
  • CD11c/CD18 binds to CD54, iC3b and fibrinogen and plays a role in leukocyte adhesive interactions. (thermofisher.com)
  • The protein intercellular adhesion molecule 3 (ICAM-3) functions as a receptor for LFA-1 (leukocyte function-associated antigen-1) and mediates adhesive interactions of leukocyte cell surface adhesion molecules in inflammatory and immune response. (sigmaaldrich.com)
  • T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. (exbio.cz)
  • This disease is a defect in fucose metabolism (lack of fucosylation of the carbohydrate selectin ligands) that results in failure to express the ligand for E and P selectin, sialyl Lewis-X (CD15s) expressed on leukocytes and endothelial cells. (medscape.com)
  • As a second ligand of leukocyte function-associated antigen-1, ICAM-2 functions as a costimulatory molecule for effector cells. (betalifesci.com)
  • Description: CD54, also known as Intercellular adhesion molecule-1 (ICAM1) is a ligand for lymphocyte function-associated (LFA) antigens. (csb-bioenergetics.nl)
  • Labial ulceration from which Escherichia coli was cultured in an 8-month-old girl with leukocyte adhesion deficiency type 1 (LAD I). Note the thin bluish scar at the superior aspect of the labia from an earlier cellulitis. (medscape.com)
  • This 3-year-old girl had leukocyte adhesion deficiency type I (LAD I) with complete absence of CD18 expression. (medscape.com)
  • This 10-month-old patient with severe leukocyte adhesion deficiency type I (LAD I) developed a cervical adenitis caused by Klebsiella pneumoniae. (medscape.com)
  • Of the 8 plasma membrane receptors for complement, only deficiencies of CR3 and CR4 due to CD18 deficiency have been described, known as leukocyte adhesion deficiency (LAD) type 1 . (medscape.com)
  • These 2 members mediate leukocyte adhesions to endothelial cells but they also serve as receptors for iC3b (inactivated C3b). (medscape.com)
  • ACKR1 is one of more than 20 distinct chemokine receptors expressed in human leukocytes. (guidetomalariapharmacology.org)
  • Human peripheral blood lymphocytes were stained with CD18 (clone CBR LFA-1/2) FITC (filled histogram) or mouse IgG1, κ FITC isotype control (open histogram). (biolegend.com)
  • The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkynje cells in the cerebellum. (exbio.cz)
  • Among splenocytes, NK cells have the highest density of CD18, and T lymphocytes express a higher density than the remaining cells. (bdbiosciences.com)
  • LFA-1 participates in several processes, such as T cell-mediated killing, T helper and B lymphocyte responses, natural killing, Ab-dependent cellular cytotoxicity by monocytes and granulocytes, and adherence of leukocytes to endothelial cells, fibroblasts, and epithelial cells. (sigmaaldrich.com)
  • Traffic signals for lymphocyte recirculation and leukocyte emigration: the multistep paradigm. (bdbiosciences.com)
  • Blockade of PLY-induced alveolar leukocyte trafficking by pretreatment of mice with anti-CD18 plus anti-CD49d Abs or depletion of circulating neutrophils did not attenuate the increase in lung permeability observed in response to intratracheal PLY. (aai.org)
  • In neutrophils these proteins are activation antigens in that their surface expression is increased following stimulation. (umn.edu)
  • Kinnaert P, Pradier O, Bournonville B, Habrant C, Goldman M, Van Geertruyden N: Role of CD18-dependent and CD18-independent mechanisms in the increased leukocyte adhesiveness and in the variations of circulating white blood cell populations induced by anti-CD3 monoclonal antibodies. (exbio.cz)
  • Nonopsonic phagocytosis of Pseudomonas aeruginoas: insights from an infant with leukocyte adhesion deficiency. (ox.ac.uk)
  • Molecular complexity of leukocyte surface glycoproteins related to the macrophage differentiation antigen Mac-1. (bdbiosciences.com)
  • CD18 and associated α chains function in the adhesion and signaling in hematopoietic cells. (biolegend.com)
  • [ 3 ] Thus, it enhances and prolongs antigen signaling on B cells. (medscape.com)
  • [ 8 ] Deficiency of CD18 on phagocytic cells causes LAD type 1 (see Table 2). (medscape.com)
  • Among CD34 + CD33 - cells, CD18 + cells gave rise to colony-forming cells (CFCs) on stromal layers, but reached a maximum at week 2, after which the number of generated CFCs decreased. (elsevierpure.com)
  • On the other hand, CD18 - cells generated less CFCs than CD18 + cells at 2 to 3 weeks, but increased after 4 weeks of culture. (elsevierpure.com)
  • CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. (exbio.cz)
  • To sustain the vascular changes and attract more immune cells to the site of inflammation, leukocytes and tissue cells secrete a range of inflammatory mediators, including interleukins and chemokines . (amboss.com)
  • The aim of this study was to determine whether infant dietary antigens could be immunizing agents of IAA. (shengsci.com)
  • Chemokines primarily act to promote leukocyte chemotaxis to sites of inflammation. (guidetomalariapharmacology.org)
  • Elimination of the causative factor by leukocytes leads to the resolution of acute inflammation and tissue repair with complete regeneration or scarring. (amboss.com)
  • Another reported type of leukocyte adhesion deficiency involves dysfunction in platelet aggregation in addition to a defect in leukocyte adhesion. (medscape.com)
  • severe reduction of CD18 expression leads to the development of a psoriasiform skin disease. (icbinhibitor.com)
  • Thus, patients with this type of leukocyte adhesion deficiency manifest both severe bacterial infections and bleeding disorder. (medscape.com)
  • Variants of leukocyte adhesion deficiency have also been reported, including fully expressed but nonfunctional CD18 and an E selectin that is expressed but rapidly cleaved from the cell surface (only present in soluble form). (medscape.com)
  • It has been determined previously that polymorphonuclear leukocytes, or PMNs, can facilitate melanoma cell extravasation through the endothelium under shear conditions [1,2]. (psu.edu)
  • 1% expression of CD18). (medscape.com)
  • In milder forms of leukocyte adhesion deficiency I (1-30% expression of CD8), patients may survive to adulthood. (medscape.com)
  • This leukocyte adhesion deficiency variant is associated with defective expression of the Rap-1 activator CalDAG-GEFI. (medscape.com)
  • Immunogen: Leukocytes of a patient suffering from a LGL-type leukemia. (icbinhibitor.com)
  • CD18 is a 90-95 kDa type I transmembrane protein expressed on all leukocytes. (icbinhibitor.com)
  • Currently, 18 subtypes have been reported: 12 are type I (dysfunctional lipid-linked oligosaccharide precursor synthesis), and 6 are type II (dysfunctional trimming/processing of the protein-bound oligosaccharide), including leukocyte adhesion deficiency II (CDG-IIc). (medscape.com)