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  • blasts
  • KEY FINDINGS: The Dg complex was altered in leukemia cells, including decreased mRNA, protein, and α-Dg glycosylated levels, mislocalization of β-Dg, and a diminution of mRNA expression of LARGE in patients leukemia blasts and in cell lines. (whiterose.ac.uk)
  • Leukemic blasts (mostly CD34 + ) from patients with acute myeloblastic leukemia (AML) expressed variable amounts of CXCR-4, which was functionally active, as demonstrated by a positive correlation between the SDF-1-induced transendothelial migration and the cell surface density of CXCR-4 (r = 0.97). (bloodjournal.org)
  • Also recombinant SDF-1β induced migration of CXCR-4-positive leukemic blasts. (bloodjournal.org)
  • Recent data suggest that the interaction between VLA-4 (α 4 β 1 integrin) on leukemic blasts and fibronectin on stromal cells activates phosphatidylinositol 3-kinase (PI3K)/Akt/Bcl-2 signaling, an important determinant of AML chemosensitivity and the level of minimal residual disease of AML patients ( 3 ). (aacrjournals.org)
  • K562
  • The kinetics of the HERG1 current in K562 cells resembled the rapid component of the native cardiac delayed rectifier current, known to be conducted by heterotetrameric HERG1 channels. (deepdyve.com)
  • Our results in K562 cells suggest the assembling of heterotetrameric channels, with some parameters being dominated by one of the isoforms and other parameters being intermediate. (deepdyve.com)
  • However, we found that K562 HERG1 whole-cell currents were not activated by H2O2. (deepdyve.com)
  • The maximal decrease of mdr1 mRNA levels was found to be 6-fold in the K562/ADR cells and 3-fold in the CEM VLB100 cells. (aspetjournals.org)
  • This study revealed that mdr1 mRNA was stable in both cell lines and no increase in mdr1 mRNA degradation was observed in the 30 microM VRP-treated cells versus control cells (half-lives of 23 hr versus 14 hr for the K562/ADR cells and 15.5 hr versus 10.0 hr for the CEM VLB100 cells). (aspetjournals.org)
  • tumor cells
  • Conventional anticancer chemotherapy is limited because of severe side effects as well as a quickly evolving multidrug resistance of the tumor cells. (springer.com)
  • However, it remains to be understood how tumor cells adhere to endothelium and infiltrate into underlying tissue. (nii.ac.jp)
  • mRNA
  • After siRNA were transfected into HL60 cells, both GINS2 expression level of mRNA and protein in interfering group were down-regulated when compared with control groups. (medsci.org)
  • By reverse transcriptase-polymerase chain reaction (RT-PCR), however, basal levels of CXCR-4 mRNA were also detected in all leukemic cell lines. (bloodjournal.org)
  • Modulations of the steady-state mRNA levels of TCL1 were analyzed by quantitative real-time PCR and Western blotting in both primary B-CLL samples and leukemic cell lines. (aacrjournals.org)
  • To determine whether the decrease of mRNA levels resulted from post-transcriptional mechanisms, mRNA stability was studied after blocking of transcription with actinomycin D in VRP-treated cells and in control cells. (aspetjournals.org)
  • Here, we demonstrate that PRL-3 expression at mRNA and protein level was higher in B-ALL cells than in normal cells, as measured by qRT-PCR or flow cytometry. (bibsys.no)
  • inhibitor
  • To test this, we developed a screening assay for evaluating the sensitivity of CTCL cells to targeted molecular agents, and compared a novel BCL2 inhibitor, venetoclax, alone and in combination with a histone deacetylase (HDAC) inhibitor, vorinostat or romidepsin. (bloodjournal.org)
  • Moreover, in all tested CML cells, with the exception of T315I mutation cells, combining JNJ-26854165 and tyrosine kinase inhibitor (TKI) Imatinib or PD180970 leads to a synergistic effect. (sigmaaldrich.com)
  • B-CLL patient samples ( n = 35) and B leukemic cell lines (EHEB, JVM2, JVM3, MEC1, MEC2, and BJAB) with different p53 status were exposed to Nutlin-3, a small-molecule inhibitor of the p53-MDM2 interaction. (aacrjournals.org)
  • We further provide evidence that ibrutinib, a Btk inhibitor that promotes mobilization of leukemic cells from SLOs, normalizes the imbalance between CXCR4/CCR7 and S1P1. (aacrjournals.org)
  • gene
  • What's more, ATM, CHK2, and P53 gene could involve in the pathogenic signaling pathways of HL60 cells when GINS2 gene was down-regulated. (medsci.org)
  • The gadd153 gene encodes the nuclear protein CHOP 10 that acts as a negative modulator of CCAAT/enhancer binding protein transcriptional factors and inhibits cell cycle progression. (aacrjournals.org)
  • The gadd153 gene was constitutively expressed in the four studied cell lines. (aacrjournals.org)
  • 0.05) upregulate the p53 target gene MDM2 in the p53 wild-type leukemic cells. (aacrjournals.org)
  • A 4-fold decrease in the mdr1 gene transcription rate was observed in the 30 microM VRP-treated CEM VLB100 cells. (aspetjournals.org)
  • The decreased transcription rate could be due to the decrease in mdr1 proximal promoter activity observed in CEM VLB100 cells transiently transfected with the mdr1 promoter fused to the chloramphenicol acetyltransferase gene. (aspetjournals.org)
  • Publications] Abe,M.: 'Regulation of interleukin (IL)-1 β gene transcription induced by IL-1 β in rheumatoid synovial fibroblast-like cells,E11,transformed with simian virus 40 large T antigen' J.Rheumatology. (nii.ac.jp)
  • egress
  • 14 One might speculate that the capability of leukemic cells to egress from the bone marrow microenvironment and circulate in the peripheral blood also depends on the chemotactic response to SDF-1. (bloodjournal.org)
  • The defective expression in these cells of the egress S1P receptor S1P1 may further contribute to the retainment of CLL cells in the prosurvival stromal niche ( 9, 10 ). (aacrjournals.org)
  • protein
  • Carbohydrates, but not protein epitopes, were destroyed by treatment of cells with 5 mM sodium m -periodate followed by neutralization with 50 mM sodium borohydride. (nih.gov)
  • ILK is a highly conserved ankyrin repeat-containing serine-threonine protein kinase that links the cell adhesion receptors, integrins, and growth factors to the downstream signaling pathways. (aacrjournals.org)
  • Results all-trans retinoic acid rapidly increased endogenous and inducible expressed or CoCl 2 -stabilized HIF-1α protein in leukemic cells under normoxia. (haematologica.org)
  • 2) Different from any other hematopoietic cells, ATL cells express a characteristic heparan sulfate capable of immobilizing heparin-binding chemokine macrophage inflammatory protein (MIP) -1beta, a potent T cell-integrin-trigger, produced by the cells themselves. (nii.ac.jp)
  • 3) Competitive interruption of endogenous heparan sulfate proteoglycan-synthesis reduces cell surface MIP-1beta and prev … More ents ATL cells from integrin-mediated adhesion to endothelial cells of ICAM-1 triggered through G-protein. (nii.ac.jp)
  • 4) The heparan sulfate proteoglycan on ATL cells characteristically consists of an 100 kDa unknown core protein and extremely weakly sulfated glycosaminoglycan, which has a potency to bind to distinctive heparin-binding cytokines. (nii.ac.jp)
  • endothelial
  • Heparan sulfate proteoglycan on leukemic cells is primarily involved in integrin-triggering and its mediated adhesion to endothelial cells. (nii.ac.jp)
  • 1) ATL cells adhere to endothelial cells through already activated integrins without exogenous stimulation. (nii.ac.jp)
  • Thus, we here propose that leukemic cells adhere to endothelial cells through the adhesion cascade, similar to normal leukocyte, and that the cell surface heparan sulfate particularly on ATL cells is pivotally involved in chemokine-dependent autocrine stimulation of integrin-triggering by immobilizing the chemokine on them. (nii.ac.jp)
  • Publications] Tanaka,Y.: 'Heparan sulfate proteoglycan on leukemic cells is primarily involved in integrin-triggering and its mediated adhesion to endothelial cells' J.Exp.Med.184巻. (nii.ac.jp)
  • Line
  • In accordance with the immunofluorescence data, CD34 + progenitors efficiently migrated across endothelium in response to SDF-1 containing conditioned medium from the stromal cell line MS-5. (bloodjournal.org)
  • In addition, transfection experiments with either p53 siRNA or with a TCL1 expression plasmid were carried out in the EHEB B-CLL cell line. (aacrjournals.org)
  • Factor XI-deficient plasma affected the time to peak values in one leukemic cell line and also attenuated peak thrombin. (ovid.com)
  • Jurkat
  • The major O-glycan carrier proteins CD43 and CD162 and isoforms of CD45 expressed on Jurkat cells were precipitated by anti- Tn mAbs with different affinities. (nih.gov)
  • For comparison, untreated Jurkat cells were stained with primary and secondary Abs (open bold histograms) or with a secondary FITC-labeled Ab only (open thin histograms). (nih.gov)
  • Parental Jurkat cells transduced with IRES GFP (see Material and methods ) express an epitope recognized by JA5 IgG mAb. (nih.gov)
  • In contrast to these cells , Jurkat cells transduced with wtCosmc IRES GFP are negative in JA5 staining. (nih.gov)
  • D ) Binding of JA mAbs to the Jurkat and Jurkat-wtCosmc cells was comparatively quantified by FACS and expressed in MFI with standard deviation error bars for triplicates. (nih.gov)
  • mechanisms
  • These results provide further identification of the regulatory mechanisms involved in the overexpression of mdr1 in MDR cells and may help in the development of new strategies for MDR reversal. (aspetjournals.org)
  • We studied mechanisms of extravasation of leukemic cells using adult T cell leukemia (ALT) cells and report the following novel features of cell surface heparan sulfate proteoglycan on ATL cells in ATL cell adhesion to endothelium. (nii.ac.jp)
  • expression
  • Further experiments revealed that down-regulation of GINS2 expression inhibited DNA replication and had a G2/M phase block in HL60 cells. (medsci.org)
  • In order to further explore the effect and mechanism of GINS2 in the progression of APL, we knocked down the expression of GINS2 by RNA interference and over-expressed GINS2 by adenovirus infection in HL60 cells. (medsci.org)
  • Additionally, we evaluated the relative expression of the main enzymes controlling α-Dg glycosylation to ascertain the post-translational modifications in the leukemia cell phenotype. (whiterose.ac.uk)
  • Migration of leukemic cells might also depend on the expression of chemokine receptors. (bloodjournal.org)
  • Therefore, we analyzed expression of CXCR-4 on mobilized normal CD34 + progenitors and leukemic cells. (bloodjournal.org)
  • Expression of CXCR-4 is also found in a variety of nonhematopoietic cells and organs. (bloodjournal.org)
  • Expression of the 130,000 molecular weight band correlates with the appearance of phagocytic and chemotactic activities of the cells. (pnas.org)
  • Blockade of PI3K or ILK signaling with pharmacologic inhibitors LY294002 or QLT0267 specifically inhibited stroma-induced phosphorylation of Akt and glycogen synthase kinase 3β, suppressed STAT3 and ERK1/2 activation, and decreased Notch1 and Hes1 expression in leukemic cells. (aacrjournals.org)
  • Circulating chronic lymphocytic leukemia (CLL) cells display an abnormal increase in the surface levels of the homing receptors CCR7 and CXCR4 concomitant with low S1P receptor 1 (S1P1) expression. (aacrjournals.org)
  • In addition, increased expression of these receptors, together with S1P1 deficiency, is detectable not only in circulating leukemic cells, but also in SLOs of CLL patients with lymphoadenopathy. (aacrjournals.org)
  • Finally, PRL-3 expression made Reh cells more resistance to cytarabine treatment. (bibsys.no)
  • In conclusion, the expression level of PRL-3 was higher in B-ALL cells than in normal cells. (bibsys.no)
  • anticancer
  • To address this problem, we have explored a C 60 fullerene-based nanosized system as a carrier for anticancer drugs for an optimized drug delivery to leukemic cells. (springer.com)
  • pathways
  • One such opportunity for the repurposing of existing therapies involves the dysregulation of B-cell lymphoma 2 (BCL2)-driven apoptotic pathways in CTCL. (bloodjournal.org)
  • Coculture of leukemic NB4 cells with bone marrow-derived stromal mesenchymal stem cells (MSC) resulted in robust activation of multiple signaling pathways, including ILK/Akt, extracellular signal-regulated kinase 1/2 (ERK1/2), signal transducers and activators of transcription 3 (STAT3), and Notch1/Hes. (aacrjournals.org)
  • mechanism
  • The ubiquitin-proteasome pathway is the principal mechanism for the degradation of short-lived proteins in eukaryotic cells. (aacrjournals.org)