• Bone marrow adherent cell types, collectively referred to as stromal cells, appear to be key players in such escape, mainly because CLL malignant cells, which rapidly undergo spontaneous apoptosis when cultured in vitro, survive, migrate, and resist cytotoxic agents in co-culture with bone marrow stromal cells. (dovepress.com)
  • Moreover, RG7356 could induce CLL cells that expressed the zeta-associated protein of 70 kDa (ZAP-70) to undergo caspase-dependent apoptosis, independent of complement or cytotoxic effector cells. (pnas.org)
  • The kinase inhibitor dasatinib induces apoptosis in chronic lymphocytic leukemia cells in vitro with preference for a subgroup of patients with unmutated IgVH genes," Blood , vol. 112, no. 4, pp. 1443-1452, 2008. (hindawi.com)
  • However, we have recently discovered that vinblastine can induce very rapid apoptosis in primary CLL cells despite the fact that they are nonproliferative ( 1, 2 ). (aacrjournals.org)
  • The ectopic expression of ILT3 in CLL was a distinctive feature of neoplastic B cells and hematopoietic stem cells, thus identifying ILT3 as a selective marker of malignancy in CLL and the first example of phenotypic continuity between mature CLL cells and their progenitors in the bone marrow. (bloodjournal.org)
  • Southern blot analysis of the bone marrow and lymph node revealed the same rearrangement of bands of T-cell-receptor genes. (springer.com)
  • The cytotoxic effect of this mAb was not mitigated when the CLL cells were cocultured with mesenchymal stromal cells (MSCs) or hyaluronic acid or when they were stimulated via ligation of the B-cell receptor with anti-µ. (pnas.org)
  • Intriguingly, these mice have a high-risk phenotype resembling the typical molecular features of an UMT BCR of human CLL cells. (aacrjournals.org)
  • Administration of this mAb at a concentration of 1 mg/kg to immune-deficient mice engrafted with human CLL cells resulted in complete clearance of engrafted leukemia cells. (pnas.org)
  • Also, we discuss similarities and differences between B-CLL and hairy cell leukemia (HCL) and suggest that also HCL may be derived from a post-GC memory or marginal zone B cell. (nih.gov)
  • HIF-1α critically regulates the interaction of neoplastic CLL cells with the leukemic microenvironment. (bloodjournal.org)
  • Mutational status of TP53 together with expression of wild-type (wt) IGHV represents the most widely accepted biomarkers, establishing a very poor prognosis in B-cell chronic lymphocytic leukemia (B-CLL) patients. (frontiersin.org)
  • RG7356 induced rapid internalization of CD44 on CLL cells at 37 °C, resulting in reduced expression of ZAP-70, which we found was complexed with CD44. (pnas.org)
  • We now report the genomewide expression profiling of miRNAs in human B cell chronic lymphocytic leukemia (CLL) by using a microarray containing hundreds of human precursor and mature miRNA oligonucleotide probes. (pnas.org)
  • These data suggest that miRNA expression patterns have relevance to the biological and clinical behavior of this leukemia. (pnas.org)
  • Little else is known about miRNA expression patterns or function in normal or neoplastic cells. (pnas.org)
  • Identification of a novel recurrent gain on 20q13 in chronic lymphocytic leukemia by array CGH and gene expression profiling," Annals of Oncology , vol. 23, no. 8, pp. 2138-2146, 2012. (hindawi.com)
  • No conventional therapy is currently curative for CLL, and although allogeneic stem cell transplantation does provide a potentially curative modality, the morbidity and mortality of this procedure remain relatively high. (psychiatryadvisor.com)
  • Moreover, we show that the sensitivity of B-CLL cells to NK cells depends on the prognosis based on TP53 and IGHV mutational status. (frontiersin.org)
  • Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation and cultured in complete RPMI 1640. (aacrjournals.org)
  • Forty-seven samples were used for this study, including 41 samples from 38 patients with CLL and 6 normal samples, including one lymph node, tonsillar CD5+ B cells from two normal donors, and blood mononuclear cells (MNC) from three normal donors. (pnas.org)
  • These signaling complexes regulate key downstream pathways such as extracellular signal-regulated kinase, c-Jun NH 2 -terminal kinase, p38, and Akt, the outcomes of which determine the B-cell fate ( 5 ). (aacrjournals.org)
  • Triggering of ILT3 inhibited the activation of Akt kinase upon B cell receptor stimulation. (bloodjournal.org)
  • The goal of these experiments was to determine the impact of vinblastine on ABT-737 sensitivity in CLL cells isolated from peripheral blood and to define the underlying mechanism. (aacrjournals.org)
  • As the proportion of cycling cells in CLL is very low, particularly in the peripheral circulation, vinca alkaloids should be inactive. (aacrjournals.org)
  • Tcl1a was initially characterized in preleukemic T cells ( 12 ) but is also expressed in subsets of naïve lymphocytes ( 13 ). (aacrjournals.org)
  • natural killer cells (CD16+) and B lymphocytes (CD19+) were purified by using magnetic beads (Dynabeads, Unipath, Milan) and specific mAbs (Becton Dickinson). (pnas.org)
  • Other experiments demonstrated that normal human B lymphocytes expressed the linker histone variants H1.2, H1.3, H1.4, and H1.5, and that B cells from patients with B-CLL expressed the same variants although in different amounts. (diva-portal.org)
  • It was also observed that the levels of linker histone phosphorylation in EBV-transformed B lymphocytes were already increased in the G1 phase of the cell cycle, which is earlier than previously thought. (diva-portal.org)
  • Freshly isolated CLL cells from 35 patients, as well as normal lymphocytes and platelets, were incubated with various microtubule-disrupting agents plus ABT-737 to assess sensitivity to the single agents and the combination. (aacrjournals.org)
  • Unrelated donor marrow transplantation for B-cell chronic lymphocytic leukemia after using myeloablative conditioning: results from the Center for International Blood and Marrow Transplant research. (acronymfinder.com)
  • Immunophenotyping of the bone marrow-infiltrating cells revealed a postthymic suppressor/cytotoxic phenotype of CD2 + , CD3 + , CD4 − , CD5 + , CD8 + , CD25 − , TCR-α β. (springer.com)
  • ILT3 is ectopically expressed on mature CLL cells and CLL progenitors in the bone marrow. (bloodjournal.org)
  • We found that a humanized mAb specific for CD44 (RG7356) was directly cytotoxic for leukemia B cells, but had little effect on normal B cells. (pnas.org)
  • We present preliminary data suggesting that B-CLL susceptibility significantly correlates with HLA mismatch between NK cell donor and B-CLL patient. (frontiersin.org)