• Feedback inhibition of acetohydroxy acid synthase isoenzyme III by l -valine was removed by site-directed mutagenesis, and the native promoter containing the transcriptional attenuator leader regions of the ilvGMEDA and ilvBN operon was replaced with the tac promoter. (pnas.org)
  • RNAs were prepared from bacteria grown in M17lac medium (lanes 1 and 3) or CDM containing all of the amino acids (lanes 2 and 4). (asm.org)
  • Scientists at the Max Planck Institute for Chemical Ecology found out recently that the ancestor of a gene involved in making chemical defenses in plants of the mustard family (Brassicaceae), such as rapeseed and cabbage, originally had a completely different function, playing a part in the formation of leucine, an essential amino acid for humans. (uni-protokolle.de)
  • In conclusion, Cj1199 gene may directly regulate the leucine biosynthesis and transport and indirectly affect the development of erythromycin resistance in C. jejuni . (frontiersin.org)
  • The systematic name of this enzyme class is acetyl-CoA:3-methyl-2-oxobutanoate C-acetyltransferase (thioester-hydrolysing, carboxymethyl-forming). (wikipedia.org)
  • The antimicrobial susceptibility, antimicrobial resistance development, growth characteristics and leucine metabolism were examined to confirm the biological function of Cj1199 . (frontiersin.org)
  • Northern analysis of the S. thermophilus wild-type (lanes 1 and 2) and IlvC-negative mutant (lanes 3 and 4) RNAs. (asm.org)
  • In addition to the complex requirements for a divalent metal and further activation by K+, M. tuberculosis α-isopropylmalate synthase follows a random kinetic mechanism for catalysis. (wikipedia.org)
  • Non-proteinogenic BCAAs include 2-aminoisobutyric acid . (wikipedia.org)
  • L-SUCCINYLAMINOACYLASE AND PROCESS FOR PRODUCING L-AMINO ACID USING IT - The present invention provides an L-aminoacylase which is able to produce L-tert-leucine being useful as an intermediate for pharmaceuticals. (patentsencyclopedia.com)
  • [2] For example, enough arginine is synthesized by the urea cycle to meet the needs of an adult but perhaps not those of a growing child. (wikipedia.org)
  • Alanine/glycine and rice powder containing ORS demonstrated some reduction in the stool output [2- (bioportfolio.com)
  • For example, randomly mutated and/or metabolically engineered E. coli strains have been used for the production of l -threonine ( 2 ), l -phenylalanine ( 9 ), and l -alanine ( 10 ). (pnas.org)
  • Alanine 2,3-aminomutases and related polynucleotides - The present disclosure relates generally to polypeptides having improved alanine 2,3-aminomutase (AAM) activity, the polynucleotides encoding the AAM polypeptides, and expression vectors and host cells for expressing the AAM polypeptides. (patentsencyclopedia.com)
  • Growth rate (μ max) obtained with the wild-type strain (open bars) and mutant 1 or 2 (solid bars) in CDM in which the nitrogen source was variable. (asm.org)
  • Mycobacterium tuberculosis α-isopropylmalate synthase requires a divalent metal ion, of which Mg2+ and Mn2+ give highest activity, and a monovalent cation, with K+ as the best activator. (wikipedia.org)
  • 2. The method of claim 1, wherein the recombinant cell exhibits an increase in activity of isocitrate lyase compared to a parent cell. (patentsencyclopedia.com)
  • citation needed] Another feature of the M. tuberculosis homolog is that L-leucine, the feedback inhibitor, inhibits the enzyme in a time-dependent fashion. (wikipedia.org)
  • 2. Reducing the concentration of glucose and sodium and thus osmolarity of ORS solution. (bioportfolio.com)
  • Four differentially protected tri-substituted dihydropyrans based on glucose were synthesised, including 2,3-unsaturated silyl glycosides and α,β-unsaturated lactones. (nzresearch.org.nz)
  • Ecotypes were grown as single plants in 5 × 5 cm 2 pots filled with a 1:3 vermiculite/standard soil (Einheitserdenwerk, Fröndenberg, Germany) mix under 11.5 h/12.5 h light/dark cycles. (pnas.org)