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  • RNAi
  • Packaging and proliferating lentivirus with RNA interference (RNAi) technique is a conserved biological response to double-stranded RNA (Hannon, 2002), RNAi is a sequence-specific process that regulates genetic functions and provides defense against virus at the post-transcriptional level in mammalian cells and animals (Song et al. (thefreedictionary.com)
  • Addgene: Lentivirus-delivered stable gene silencing by RNAi in primary cells. (addgene.org)
  • shRNA
  • We are trying to knockdown a gene in primary hepatocytes using lentivirus shRNA from Sigma. (protocol-online.org)
  • Cells that were not treated with lentivirus, but underwent puromycin selection, demonstrated no resistance to puromycin and did not survive after selection (A). However, when cells were transduced with either empty vector lentivirus, SHC001V (B) or non-target shRNA lentivirus, SHC002V (C), both express the puroR gene once integrated, the cells survive and proliferate without any morphological abnormalities after selection. (sigmaaldrich.com)
  • Titers
  • We also describe limitations to the lentivirus system including technical challenges due to low titers and low infection efficiency in primary cultures. (nih.gov)
  • vitro
  • Previously two phase I human studies based on in vitro transfer of the cDNA via lentivirus to arthritic joints have confirmed its feasibility and safety in human subjects. (nih.gov)
  • Polysaccharide-modified scaffolds for controlled lentivirus delivery in vitro and after spinal cord injury. (nih.gov)
  • The addition of chitosan and heparin onto PLG promoted the association of lentivirus to these scaffolds and enhanced their transduction efficiency in vitro relative to hyaluronan-conjugated and control scaffolds that had limited lentivirus association and transduction. (nih.gov)
  • To investigate hierarchy in human prostate epithelial cells, we generated recombinant lentiviruses, infected primary cultures and cell lines, and followed their fate in vitro. (nih.gov)
  • proteins
  • We systematically screened publicly available, genome scale sequence databanks and trace archives using BLAST algorithms and sequence probes derived from the enzymatic and structural proteins of representative lentiviruses. (pnas.org)
  • The lentiviruses combined constitutive promoters including CMV and β-actin, or late-stage differentiation promoters including PSCA (prostate stem cell antigen) and PSAPb (prostate specific antigen/probasin) driving expression of monomeric, dimeric and tetrameric fluorescent proteins. (nih.gov)
  • integrate
  • Lentiviruses can integrate a significant amount of viral cDNA into the DNA of the host cell and can efficiently infect nondividing cells, so they are one of the most efficient methods of gene delivery . (wikipedia.org)
  • By quantifying the number of lentiviruses that have integrated into the nuclear DNA of your target cells, you can precisely determine the real titer (i.e., effective titer) of your lentiviral supernatant stock and use this information to infect cells with greater precision and more highly defined MOI (multiplicity of infection), allowing you to predict how many viral genomes will integrate into your cells. (clontech.com)
  • RELIK
  • The discovery of RELIK extends the host range of lentiviruses to a fifth mammalian order and demonstrates that such viruses can also spread by vertical transmission and intragenomic proliferation, in addition to infectious horizontal transfer. (pnas.org)
  • genomic
  • Evolutionary analyses of the genomic sequences of modern-day lentiviruses have suggested a relatively recent date for their emergence, but the failure to identify any endogenous, vertically transmitted examples has meant that their longer term evolutionary history and origin remain unknown. (pnas.org)
  • Thus, the lack of endogenous "genomic fossils" has precluded studies into the longer term evolutionary history and origin of lentiviruses ( 14 , 15 ). (pnas.org)
  • vivo
  • Before using lentivirus with sensitive target cells or for in vivo applications, it is essential to remove these contaminants by purifying the lentivirus. (clontech.com)
  • Transduction efficiency was also evaluated in vivo using porous, multiple channel PLG bridges that delivered lentivirus to the injured mouse spinal cord. (nih.gov)
  • Transduction
  • Lentivirus purification enables you to remove cellular contaminants that could otherwise adversely affect your transduction experiments. (clontech.com)
  • Using purified lentivirus stocks also prevents 'pseudo-transduction', where high levels of recombinant protein in the crude supernatant are passively transferred to target cells during infection. (clontech.com)
  • infect
  • Consequently, we hypothesis that directly infect the chondrocytes and synoviocytes with lentivirus carrying the fat-1 gene could be a well therapeutic strategy for OA in humans. (nih.gov)
  • cells
  • Lentivirus is a perfect tool for screening applications since the delivered genetic material is constitutively expressed by the cells long-term. (sigmaaldrich.com)
  • The Lenti-X Provirus Quantitation Kit allows you to rapidly determine the copy number of integrated lentiviruses (proviruses) present in a mixed or clonal population of transduced cells. (clontech.com)
  • Does anybody has the experience in transfection of mES cells (fedder dependent) using lentivirus? (protocol-online.org)
  • I am sure that you are just a phone call (to whoever sold you the lentivirus) to know which cells to use. (biology-online.org)
  • Lentiviruses also infected CD133(+) cells within the P4E6 cell line. (nih.gov)
  • infection
  • Also, for a given virus prep, its effective MOI across different target cell lines can vary widely depending on each cell line's individual susceptibility to lentivirus infection. (clontech.com)
  • bridges
  • Herein, we investigated long-term local immunomodulation using anti-inflammatory cytokine IL-10 or IL-4-encoding lentivirus delivered from multichannel bridges. (rutgers.edu)
  • virus
  • Although the virus we describe is defective, reconstruction of an infectious progenitor could provide novel insights into lentivirus biology and host interactions. (pnas.org)
  • however
  • However, non-integrating lentivirus can be produced through the use of mutations in the integrase protein that minimize proviral integration. (clontech.com)
  • Five
  • Five serogroups of lentiviruses are recognized, reflecting the vertebrate hosts with which they are associated (primates, sheep and goats, horses, domestic cats, and cattle). (wikipedia.org)