Lentiviral vector encoding. Medical search. Frequent questions

We recently developed a lentiviral vector system to study host cell entry mediated by the spike protein of MERS-CoV (MERS-S) ( 5 ). (cdc.gov)
We have developed a lentiviral vector system based on the HIV that can transduce terminally differentiated neurons of the brain in vivo. (nih.gov)

In this study, we tested whether preventative and therapeutic vaccination could be achieved by direct injection of antigen-expressing lentiviral vector, obviating the need for ex vivo transduction of dendritic cells. (jci.org)
Vaccination by direct injection of lentiviral vector was more effective in sterile alpha motif and HD-domain containing protein 1-knockout (SAMHD1-knockout) mice, suggesting that lentiviral vectors containing Vpx, a lentiviral protein that increases the efficiency of dendritic cell transduction by inducing the degradation of SAMHD1, would be an effective strategy for the treatment of chronic disease in humans. (jci.org)
We show that knock-down of each single gene from multiple target vectors is achieved at an efficiency comparable to that obtained after transduction using single target viral vectors. (ejbiotechnology.info)
To increase the transduction efficiency of lentiviral vectors, high-throughput screening is used to identify compounds that could strengthen the transduction of target cells. (johnstonlabsjsu.com)
When K562 cells were transduced with a fVIII encoding lentiviral vector, the transduction was four times greater in the presence of PMA. (johnstonlabsjsu.com)
FasT CAR platform are able to concurrently activate and transduce resting T cells into a single "concurrent activation-transduction" step using XLenti vectors derived from lentivirus. (biomedcentral.com)
We provide detailed protocols for lentiviral transduction, neural differentiation, and subsequent analysis of human embryonic stem cells. (lu.se)
We provide detailed protocols for the generation of lentiviral vectors, the transduction of hES-cells, and their differentia- tion into neurons. (lu.se)

Lentiviral CRISPR vector encoding a Cas9 nickase mutant and a puromycin resistance marker expressed from the human CMV promoter. (snapgene.com)
Lentiviral CRISPR vector encoding Cas9 and the green fluorescent protein ZsGreen expressed from the human CMV promoter. (snapgene.com)

An HIV vector containing a gene encoding the green fluorescent protein (GFP) was injected into the subretinal space of rat eyes. (nih.gov)
When pseudotyped lentiviral vectors encoding green fluorescent protein (GFP) were incubated with mouse spermatozoa, these sperm were highly successful in producing transgenics. (science20.com)
Preparation and Characterization of EpCAM-Specific CAR-NK-92 Cells A second-generation CAR, consisting of EpCAM-specific scFv linked to a CD8 hinge and transmembrane domains and the KW-6002 biological activity intracellular signaling domains of 4-1BB and CD3in sequence (Figure 1(a)), was KW-6002 biological activity constructed and inserted into a lentiviral vector system with sequences encoding green fluorescent protein (GFP). (bioskinrevive.com)

In this study, we have evaluated the ability of HIV vectors to transfer genes into retinal cells. (nih.gov)
Using a lentiviral vector containing either H-Ras oncogene and a hairpin targeting p53 or both tumor suppressor genes, NF-1 and p53, Dr. Friedmann-Morvinski can induce the formation of a glioma tumor in mice injected in the brain. (genengnews.com)
In a mouse model, introducing new genetic material via a viral vector into the sperm of mice leads to the presence and activity of those genes in the resulting embryos. (science20.com)
Therapeutic Candidate or Device HSC gene modified by a lentiviral vector which encodes a triple combination of HIV-resistance genes and a pre-selective marker. (ca.gov)
The ability to inhibit two or more genes simultaneously from one single expression vector further widens the application spectrum of RNAi, both in functional studies and therapeutic strategies. (ejbiotechnology.info)
Analyzing 5′-upstream non-protein-encoding regions of the human mitochondrial function-associated genes, we speculate that mitochondrial functions could be recovered or improved at a transcriptional level. (intechopen.com)
This consideration should be applied to all genes expressed as amphotropic and polytropic pseudotyped retroviral vectors. (southalabama.edu)
The late transcribed genes are L1-L5, encoding viral capsid components (Fig. 7B). (genemedi.com)
Although these systems aren't known completely, research survey that appearance of cyst encoding protein and genes is normally upregulated, like the Wnt/-catenin pathway functionally. (globaltechbiz.com)

Lentiviral vector-mediated doxycycline-inducible USP39 shRNA or cDNA expression in triple-negative breast cancer cells. (arizona.edu)

Lentiviral vectors encoding antigens are promising vaccine candidates because they transduce dendritic cells (DC) in vivo and prime CTL responses. (elsevierpure.com)

Injected lentiviral vector preferentially transduced splenic dendritic cells and resulted in long-term expression. (jci.org)
NK cells contribute to cancer immune surveillance not only by their direct natural cytotoxicity which is triggered rapidly upon stimulation through germline-encoded cell surface receptors, but also by modulating T-cell mediated antitumor immune responses through maintaining the quality of dendritic cells and enhancing the presentation of tumor antigens. (frontiersin.org)

Therapeutic Candidate or Device The therapeutic product candidate is autologous CD34+ hematopoietic stem/progenitor cells (HSPC) transduced with the G1XCGD lentiviral vector. (ca.gov)
Having previously shown that a hybrid human/porcine fVIII transgene had 100-fold greater expression than wild type human fVIII we delivered this transgene by lentiviral vectors (LVs) to hematopoietic stem cells for gene therapy of hemophilia A. Up to 6-100 fold expression of fVIII in the HSCs were shown compared to wild type. (johnstonlabsjsu.com)

Several features of lentiviral vectors have made them advantageous for the expression of therapeutic proteins in vivo. (jci.org)
We found that IFITM proteins contribute to the IFNα-mediated protection of SAMHD1 by blocking VSV-G-mediated entry of the lentiviral particles delivering Vpx. (biomedcentral.com)
Many of these ISGs encode proteins that have antiviral activity, inhibiting viruses at various steps of their life cycle including at the earliest stages of entry [ 1 ]. (biomedcentral.com)

We used a lentiviral vector bearing the viral spike protein to detect neutralizing antibodies against Middle East respiratory syndrome coronavirus (MERS-CoV) in persons from the Eastern Province of Saudi Arabia. (cdc.gov)
These are then transfected with a lentiviral vector that encodes for a functional Wiskott-Aldrich syndrome protein. (wikipedia.org)
We have generated a lentiviral vector-based system for expression of multiple shRNAs from a single viral vector, which also encodes an EGFP reporter protein. (ejbiotechnology.info)

In order to establish a rapid, stable and reliable method for dopamine receptor agonist screening, this study used the human dopamine receptor 2 (DRD2) gene fused with a circular permuted EGFP (cpEGFP) to construct a recombinant gene, packaged with lentiviral vector, and the vector replaced the parted inner transmembrane domain of the third intracellular loop (ICL3) of genetically-encoded GPCR-activation based (GRAB) sensors. (bvsalud.org)

Puromycin-resistant lentiviral control shRNA vector, pLKO.1 induces unexpected cellular differentiation of P19 embryonic stem cells. (arizona.edu)

Injection of a lentiviral vector encoding an MHC class I-restricted T cell epitope of lymphocytic choriomeningitis virus (LCMV) and CD40 ligand induced an antigen-specific cytolytic CD8 + T lymphocyte response that protected the mice from infection. (jci.org)
The injection of chronically infected mice with a lentiviral vector encoding LCMV MHC class I and II T cell epitopes and a soluble programmed cell death 1 microbody rapidly cleared the virus. (jci.org)
These cells were transplanted into hemophilia A mice with virus based vectors and resulted in long term expression at therapeutic levels. (johnstonlabsjsu.com)
Direct injection of the OVA-, Ii-OVA-, or TfR-OVA-expressing vectors into mice resulted in a CD4 + T cell response, as shown by expansion of adoptively transferred OT-II T cells and upregulation of CD44 on these cells. (elsevierpure.com)
The Ii-OVA vector was the most potent inducer of IFN-γ-secreting CD4 + and CD8 + T cells and was the only vector to protect mice completely from challenge with OVA-expressing tumor cells. (elsevierpure.com)

Intradermal injection of lentiviral vectors corrects regenerated human dystrophic epidermolysis bullosa skin tissue in vivo. (medscape.com)

Lentiviral vectors can be used to deliver shRNAs, thereby providing the ability to infect most mammalian cell types with high efficiency, regardless of proliferation state. (arizona.edu)
Lentiviral inducible shRNA vectors are readily available, but unfortunately the process of cloning, screening, and testing shRNAs can be time-consuming and expensive. (arizona.edu)
Conclusions: Our aim was twofold: first, to take a very useful shRNA vector and make it more amenable for molecular cloning and, secondly, to provide a streamlined protocol and rationale for cost-effective design, cloning, and screening of shRNAs. (arizona.edu)

We generated chimeric recombinant SARS-CoV-2 encoding the S gene of Omicron in the backbone of an ancestral SARS-CoV-2 isolate and compared this virus with the naturally circulating Omicron variant. (biorxiv.org)

Upon histologic examination, GFP markers encoded in the lentiviral vector show up along with disease tissue that reveals GBM, grade 4. (genengnews.com)
Gene therapy represents a promising alternative, but treating epilepsy in this way involves irreversible changes to brain tissue, so vector design must be carefully optimized to guarantee safety without compromising efficacy. (ucl.ac.uk)

Lentiviral vectors were constructed to get an efficient expression of anti-Cripto-1 siRNA in CNE-2 and C666-1 cells, with invalid RNAi sequence as control. (biomedcentral.com)
An optimized lentiviral vector system for conditional RNAi and efficient cloning of microRNA embedded short hairpin RNA libraries. (arizona.edu)

Here we describe how microRNA-regulated lentiviral vectors can be used to visualize specific cell populations by exploiting endogenous microRNA expression patterns. (lu.se)

We set out to develop an epilepsy gene therapy vector optimized for clinical translation. (ucl.ac.uk)
In the bigger picture, these new treatment options need to be weighed against the possibility of cure of hemophilia through gene therapy: two adeno-associated virus (AAV) vector-based products have been approved for clinical use, and more are in the pipeline. (cdc.gov)

After finding that the hybrid human/porcine fVIII expression was enhanced by the SIV system the construction of an enhanced vector with a CMV promoter proceeded. (johnstonlabsjsu.com)
Lastly, we display the robustness of the system with a doxycycline titration and recovery time course and provide a cost/benefit analysis comparing our system with purchasing pre-designed shRNA vectors. (arizona.edu)

It was shown that an optimized vector would contain the HP-fVIII transgene driven by a CMV internal promoter within a SIV-based lentiviral backbone lacking a WPRE. (johnstonlabsjsu.com)

The HEK293T cells were infected with viral vector, screened by puromycin to select highly expressed cells. (bvsalud.org)

Alternatively, the DCs are transduced with a viral vector that expresses the antigen. (jci.org)
Herein we used a single bicistronic lentiviral vector to develop a new combination immunotherapy that consists of human anti-carbonic anhydrase IX (CAIX)-targeted chimeric antigen receptor (CAR) T cells engineered to secrete human anti-programmed death ligand 1 (PD-L1) antibodies at the tumor site. (oncotarget.com)

Using lentiviral constructs, stable gene silencing is established both in dividing and non-dividing cells. (ejbiotechnology.info)

Moreover, siRNA-mediated knockdown of KLF5 blocked the expression of CDX2, MUC2 and villin, but transfection of a KLF5 expression vector into esophageal epithelial cells promoted their transdifferentiation into columnar-like cells, as demonstrated by increased expression of the intestinal markers CDX2, MUC2 and villin. (jcancer.org)
The gene encoding the voltage-gated potassium channel Kv1.1, KCNA1, was codon-optimized for human expression and mutated to accelerate the channels' recovery from inactivation. (ucl.ac.uk)
Generation of an Optimization of a Lentiviral Vector Encoding a High Expression FVIII Transgene for the Gene Therapy of Hemophilia A. (johnstonlabsjsu.com)
Lentiviral vectors encoding tetracycline-dependent repressors and transactivators for reversible knockdown of gene expression: a comparative study. (arizona.edu)

Therefore, we sought to refine a popular vector (Tet-pLKO-Puro) and streamline the cloning process with efficient protocols so that researchers can more efficiently utilize this powerful tool. (arizona.edu)

The efficient gene transfer into photoreceptor cells by HIV vectors will be useful for gene therapy of retinal diseases such as retinitis pigmentosa. (nih.gov)

The gene, which is called MYBL2 , encodes a transcription factor that helps to control the cell division cycle. (elifesciences.org)
Lentiviral vector-based dendritic cell vaccines induce protective T cell responses against viral infection and cancer in animal models. (jci.org)
These properties have made lentiviral vectors advantageous for use in dendritic cell (DC) vaccines. (jci.org)
The NK-92 cell line was KW-6002 biological activity transduced with the EpCAM-specific CAR and empty lentiviral vector to generate CAR-NK-92 and Ctrl-NK-92 cells, respectively. (bioskinrevive.com)
For improved safety, this engineered potassium channel (EKC) gene was packaged into a non-integrating lentiviral vector under the control of a cell type-specific CAMK2A promoter. (ucl.ac.uk)
In addition, we generated EZ-Tet-pLKO vectors with hygromycin or blasticidin resistance to provide greater flexibility in cell line engineering. (arizona.edu)

This study focused on optimizing self-inactivating (SIN) lentiviral vector systems by analyzing the efficacy of various lentiviral components with the human/porcine fVIII molecule from a previous study. (johnstonlabsjsu.com)

DNA vector-based RNA interference to study gene function in cancer. (arizona.edu)

When packaged into an adeno-associated viral vector (AAV2/9), the EKC gene was also effective at suppressing seizures in a male rat model of temporal lobe epilepsy. (ucl.ac.uk)

Furthermore, we provide a detailed guide for utilizing these vectors, including shRNA design strategy and simplified screening methods. (arizona.edu)

Middle panel: general structure of an early region 1 (E1)-deleted Ad vector. (genemedi.com)
Our primary modification was to shrink the stuffer region, which allows vector purification via polyethylene glycol precipitation thereby avoiding the need to purify DNA through agarose. (arizona.edu)

Anatomy34

Organisms39

Diseases11

Chemicals and Drugs52

Analytical, Diagnostic and Therapeutic Techniques and Equipment38

Phenomena and Processes48

Disciplines and Occupations1

Information Science4

Health Care4

Developed a lentiviral vector system2

Transduction8

CRISPR2

Green fluores3

Genes9

Doxycycline inducible1

Transduce1

Dendritic cells2

Hematopoietic2

Proteins3

Protein3

EGFP1

Embryonic stem1

Mice5

Vivo1

ShRNAs3

Recombinant1

Tissue2

RNAi2

MicroRNA1

Clinical2

System2

Backbone1

Puromycin1

Antigen2

Stable1

Expression4

Protocols1

Gene transfer1

Cell6

Human1

Study1

Effective1

Design1

Region2