Lectins bind carbohydrates. Medical search. Frequent questions

Besides their specificity, lectins are excellent reagents because of their lower cost and indigenous production. (nih.gov)
The M-cell specificity of the lectin binding was further demonstrated by localization of horseradish peroxidase (HRP)-conjugated lectins under the transmission electron microscope (TEM). (nih.gov)

For structural analysis of asparagine-linked carbohydrates, sugars are released from the protein backbone by enzymes such as PNGase F ( NEB #P0704 ). (neb.com)
Intracellular lectins and glycan-modifying enzymes mediate autophagy and control host immunity and inflammation. (nature.com)
A number of inert ligands which were not expected to react with nor bind to any of the targeted enzymes or proteins were also included on the chip for comparison (see figure 2). (uchicago.edu)
After dipping the patterned chip in several solutions of different proteins or enzymes, the researchers observed that, in each case, selective binding or selective reaction occurred on the biochip. (uchicago.edu)
After his stay at the USDA he spent three years at Cornell University at the Department of Food Science working with Prof. John Brady on a multitude of projects including: the simulation of carbohydrates interacting with each other and with proteins, either as substrates for enzymes, ligands for carbohydrate-binding proteins like lectins, or in general non-specific interactions, and the hydration of planar hydrophobic species. (bradley.edu)
Some carbohydrate-metabolizing proteins (ENZYMES) from PLANTS also bind to carbohydrates, however they are not considered lectins. (bvsalud.org)

CD161 molecules are C-type lectin-like receptors that can either activate (CD161a) or inhibit (CD161b) effector leucocyte responses, eg, cytotoxicity or cytokine production, against target cells which express C-type lectin-like related (Clr) molecules. (bdbiosciences.com)
Researchers in the Netherlands, Cuba, and Italy have made important discoveries about the carbohydrate-mediated binding of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike protein to host cell lectin receptors and host lung microbiota. (news-medical.net)
ArtinM is a D-mannose carbohydrate-binding lectin that interacts with phagocytic cell receptors inducing the production of pro-inflammatory mediators related to the antitumor immune response. (usp.br)

This study reports the conjugation of a galactose-binding lectin (BmoLL) isolated from Bauhinia monandra leaves with quantum dots (QDs) by adsorption. (bvsalud.org)

The chelating binding mode accounts for the high affinity of this protein-carbohydrate interaction. (nature.com)
Agarose bound Sambucus nigra lectin is prepared using our affinity-purified lectins. (vectorlabs.com)
Intradomain allosteric network modulates calcium affinity of the C-Type lectin receptor langerin. (mpg.de)
Extracellular regions of CD206 include an N-terminal cysteine-rich (CR) domain that binds sulfated glycoproteins, a fibronectin II (FNII) domain that binds collagens, and eight carbohydrate recognition domains (CRDs) that bind sugars like mannose and fucose with high affinity. (fishersci.com)

Peanut agglutinin (PNA), Polybrene, and Glycine max lectins are frequently used for the identification of different cryptantigens. (nih.gov)
Wheat germ agglutinin and peanut agglutinin displayed heterogeneous staining patterns, binding most avidly to the intestine goblet cells. (nih.gov)
The resulting oligosaccharide derivatives were tested for binding to Erythrina cristagalli lectin (ECL), Aleuria aurantia lectin (AAL) and Ulex europaeus agglutinin-I (UEA I). We found that ligands containing an aryl azido photoactivable group were successfully attached to lectins. (uzh.ch)

The emergence of functional studies on animal lectins during the 1990s has provided the appropriate framework to better understand their roles in cell death. (nature.com)
Rat natural killer cell antigen, NKR-P1, related to C-type animal lectins is a carbohydrate-binding protein. (bdbiosciences.com)

Structure and ligand binding of carbohydrate-binding module CsCBM6-3 reveals similarities with fucose-specific lectins and "galactose-binding" domains. (expasy.org)
Moreover, a fucose-specific lectin was capable of binding to some of the abnormal haptoglobin fractions, mainly those found in association with anaemia. (lu.se)

A handful of lectins may be considered excellent reagents for anti-A, anti-B, anti-N etc, but the anti-A and anti-M are not yet regarded as commercially suitable antisera. (nih.gov)

Many plant lectins change the physiology of the membrane of BLOOD CELLS to cause agglutination, mitosis, or other biochemical changes. (bvsalud.org)

Research studies illustrate that the lectins within the serum of A. chalcodes have the ability to bind to sialic acid. (wikipedia.org)
However, the implications of the lectins binding to sialic acid must be investigated further. (wikipedia.org)
Sambucus nigra lectin, isolated from elderberry bark, binds preferentially to sialic acid attached to terminal galactose in α-2,6 and to a lesser degree, α-2,3 linkage. (vectorlabs.com)
This lectin appears to bind sialic acid linked to N -acetylgalactosamine or galactose. (vectorlabs.com)

A single glucuronic acid-β1,3-xylose disaccharide repeat straddles a Ca 2+ ion in the LG4 domain, with oxygen atoms from both sugars replacing Ca 2+ -bound water molecules. (nature.com)
Carbohydrates with the chemical formulas C n (H 2 O) n or C n (H 2 O) n-1 are commonly known as sugars or saccharides. (biosyn.com)
The analysis of these sugars can be reached using nanoprobes composed by lectins associated with fluorescent nanoparticles. (bvsalud.org)

Defining the interaction of human soluble lectin ZG16p and mycobacterial phosphatidylinositol mannosides. (mpg.de)

Glycobiology is the study of the structure, function and biology of carbohydrates, also called glycans, which are widely distributed in nature. (neb.com)
Glycans, either alone or complexed with glycan-binding proteins, can deliver intracellular signals or control extracellular processes that promote initiation, execution and resolution of cell death programs. (nature.com)
Herein, we review the role of glycans and glycan-binding proteins as essential components of the cell death machinery during physiologic and pathologic settings. (nature.com)
Endogenous lectins and glycans are critical signals in the resolution of cell death. (nature.com)
Although lectins were already known because of their ability to agglutinate red blood cells, 3 the involvement of lectins and glycans in PCD had not been elucidated. (nature.com)
Two decades later, pioneering studies suggested that lectin-like molecules constitutively expressed on the surface of macrophages can selectively recognize changes on glycans decorating the surface of apoptotic thymocytes, 4 , 5 although these studies likewise did not provide substantial insight into the mechanisms by which lectin-glycan interactions regulate cell death. (nature.com)
The research group demonstrated that biotinylated glycans enable the production of glycan arrays to determine specific interactions of lectins. (biosyn.com)
Furthermore, the study showed that fluorescent beads coated with selected biotinylated glycans bind to dendritic cell-specific ICAM-3-grabbing nonintegrin (DC-SIGN)-expressing dendritic cells in vitro. (biosyn.com)

The conjugation of cyclic carbamate-derivatized oligosaccharides with photoactivable aryl azido groups thus represents a convenient approach to study protein-carbohydrate interactions. (uzh.ch)

May function in innate immunity through activation of the lectin complement pathway. (xenbase.org)

Despite the broad occurrence of carbohydrate-protein interactions in biology, the low binding affinities of such interactions hamper the characterization of carbohydrate binding sites in the absence of three-dimensional structural models. (uzh.ch)

Since noroviruses attach to carbohydrates of the histo-blood group family, tests using immunohistochemical analysis were performed to evaluate specific binding of virus or viruslike particles to oyster tissues through these ligands. (cdc.gov)

Legume Lectins: Structures, Functions, and Carbohydrate-binding Specificities. (gr.jp)
Carbohydrates perform important physiological functions in eukaryotic and prokaryotic cells. (bvsalud.org)

At that time, glycobiology, which is the study of carbohydrates and their recognition by motif-specific carbohydrate-binding proteins or lectins, lagged far behind the studies that defined the structural and cellular biology of cell death. (nature.com)
To allow the identification of proteins interacting with specific carbohydrate epitopes, we have developed new photoactivable oligosaccharide probes. (uzh.ch)
The encoded protein binds specific carbohydrate moieties found on lipids and on the surface of microorganisms. (lsbio.com)

They are characterized by specific binding to carbohydrate residues, whether monosaccharides, disaccharides or polysaccharides. (nih.gov)
They stimulate disease-fighting cells by binding to their sugar residues, inducing the release of potent immune-system proteins called cytokines. (canadianpoultrymag.com)
NH2-terminal sequencing of the abundant precursors confirmed that the intact subunit of concanavalin A was formed by the reannealing of two fragments, since the alignment of residues 1-118 and 119-237 was reversed in the final form of the lectin identified in the chase and the precursor first labeled. (rupress.org)

This project aims to develop new carbohydrate-functionalised polymeric surfaces which will inhibit bacterial adhesion. (qub.ac.uk)
Additionally, these materials will be further modified to possess sensing capabilities, such that binding of bacterial lectins to their surface will produce a visual colourimetric change. (qub.ac.uk)
Mutants functionally deficient in vivo show no binding to K30 polymer in vitro.Apparently, Wzi is a novel outer-membrane lectin that assists in the formation of the bacterial capsule via direct interaction with capsular polysaccharides. (tcdb.org)

Simple carbohydrates are monosaccharides. (biosyn.com)
In: Modern synthetic methods in carbohydrate chemistry: from monosaccharides to complex glycoconjugates, pp. 335 - 372 (Eds. (mpg.de)

Mass spectrometric analysis confirmed the covalent binding of between one to three oligosaccharide probes, which matched with the expected carbohydrate-binding properties of the lectins tested. (uzh.ch)

Synthesis of parasite related carbohydrates and their use as probes for glycobiology. (mpg.de)

The atomic details of matrix binding were revealed by a high-resolution crystal structure of laminin-G-like (LG) domains 4 and 5 (LG4 and LG5) of laminin-α2 bound to a LARGE-synthesized oligosaccharide. (nature.com)
Figure 3: Crystal structure of laminin-α2 LG4-5 bound to oligosaccharide G6/7. (nature.com)
Figure 4: NMR analysis of binding of oligosaccharide G5 to laminin-α2 LG4-5. (nature.com)
A multifunctional galactin initially discovered as a macrophage antigen that binds to IMMUNOGLOBULIN E , and as 29-35-kDa lectin that binds LAMININ . (online-medical-dictionary.org)

Protein-carbohydrate interactions are essential in numerous biological processes. (biosyn.com)
For the study of protein-carbohydrate interactions, Grün et al. (biosyn.com)

Anti-idiotypic antibody mimicking a T-antigen-specific lectin inhibits human epithelial tumor cell proliferation. (conicet.gov.ar)
Activation of the complement system in response to an infection or foreign antigen is achieved via three complement pathways, the classical pathway, which is activated by antigen-antibody complexes, the lectin pathway, which is activated by the interaction of microbial carbohydrates with mannose-binding proteins in the plasma and tissue fluids, and the alternative complement pathway, which is activated by C3b binding to microbial surfaces and to antibody molecules. (hindawi.com)

Recommended product for eluting glycoconjugates bound to this agarose-lectin: Glycoprotein Eluting Solution, Cat. (vectorlabs.com)
Protein or glycoprotein substances of plant origin that bind to sugar moieties in cell walls or membranes. (bvsalud.org)

Viral particles bind specifically to digestive ducts (midgut, main and secondary ducts, and tubules) by carbohydrate structures with a terminal N-acetylgalactosamine residue in an α linkage (same binding site used for recognition of human histo-blood group antigens). (cdc.gov)

Lectins are carbohydrate-binding proteins found in animals and plants. (canadianpoultrymag.com)
The amino acid sequence of MytiLec is unusual, but 3-dimensional structural analysis reveals the presence of β-trefoil fold, a well-known feature in "R-type" lectins, a family of galactose-binding proteins found in many types of organisms. (go.jp)

Individual galectins differ in their tissue distribution and in their carbohydrate-binding specificities. (rndsystems.com)

KLRF1, an activating homodimeric C-type lectin-like receptor (CTLR), is expressed on nearly all natural killer (NK) cells and stimulates their cytoxicity and cytokine release (Kuttruff et al. (nih.gov)
A platform to screen for C-type lectin receptor-binding carbohydrates and their potential for cell-specific targeting and immune modulation. (mpg.de)
Complement C3b is the major opsonin of the complement system which facilitates the phagocytosis process by coating antigens (each of the phagocytes expresses a complement receptor such as CR1, CR3, or CR4 that binds C3b, C4b, or C3bi) [ 28 , 29 ]. (hindawi.com)

The rMOA-Biotin shows significant interaction with Cetuximab possessing [Galα(1-3)Gal] glycan epitopes by lectin-ELISA analysis. (tcichemicals.com)

2013). Wzi is an outer membrane lectin that underpins group 1 capsule assembly in Escherichia coli . (tcdb.org)

SFTPA1 / Surfactant Protein A is a lung surfactant protein that is a member of a subfamily of C-type lectins called collectins. (lsbio.com)

These carbohydrates not only provide a means to identify mouse M-cells by LM but may also contribute to the occurrence of specific interactions between microorganisms and the M-cell apical membrane. (nih.gov)
We discuss here the contribution of glycan-lectin interactions to the initiation, execution and resolution of apoptosis and their emerging roles in other cell death programs including autophagy. (nature.com)

Although, commonly the fungal lectins have not been characterized and expressed in recombinant form, fact that is essential to evaluate the therapeutic potential of fungal lectins. (fapesp.br)

These results reveal a previously uncharacterized mechanism of carbohydrate recognition and provide a structural framework for elucidating the mechanisms underlying muscular dystrophy. (nature.com)
Figure 5: Structural comparison of α-DG-binding LG domains. (nature.com)

Binds to GalNAc and GlcNAc carbohydrate moieties (PubMed:12679857). (xenbase.org)

This lectin preferably binds to a [Galα(1-3)Gal] glycan epitope. (tcichemicals.com)
Understanding the function of lectin-glycan recognition systems in cell death will facilitate the implementation of novel therapeutic strategies aimed at controlling unbalanced cell proliferation and survival in several pathologic conditions. (nature.com)

Galectins contain either one or two carbohydrate recognition domains (CRR) which mediate recognition of N-acetyl-lactosamine-containing glycoproteins. (rndsystems.com)
This protein is composed of three tandem repeat domains homologous to the SUEL lectin domain. (embl.de)

Metabolic labeling of immature jackbean cotyledons with 14C-amino acids was used to determine the processing steps involved in the assembly of concanavalin A. Pulse-chase experiments and analyses of immunoprecipitated lectin forms indicated a complex series of events involving seven distinct species. (rupress.org)

Lectins are unique proteins of varying biological importance. (nih.gov)
In this sense, we identified two possible lectins from P. brasiliensis and we intend to investigate the biological activities of these proteins. (fapesp.br)
Besides, the study of lectins from P. brasiliensis opens perspectives to identify other biological activities, as antitumor and antiproliferative activity, performed by different lectins already known and well characterized. (fapesp.br)

Consideration of the tertiary structure of the glycosylated precursor and mature lectin showed that the entire series of processing events could occur without significant refolding of the initial translational product. (rupress.org)

Once administered, the lectins spur a protective reaction against the disease in the gut. (canadianpoultrymag.com)
Factor I cleaves C3b and C4b in a reaction, where fI is dependent on various cofactors, such as factor H, C4b-binding protein CR1 and membrane cofactor protein (MCP) [ 26 ]. (hindawi.com)
The photoactivation reaction preserved lectin integrity, as no sign of protein degradation was visible. (uzh.ch)

These type II transmembrane glycoproteins have an extracellular C-type lectin domain and thus belong to the C-type lectin superfamily. (bdbiosciences.com)

According to the lectin-blotting analysis, the rMOA-Biotin specifically binds to α(1-3)Gal-terminated Cetuximab but not to non-α(1-3)galactosylated glycoproteins such as human transferrin (hTF), asialo hTF, agalacto hTF and bovine RNase B. (tcichemicals.com)

We tested the hypothesis that gastric co-administration of subthreshold doses of lectins and paraquat can recreate the pathology and behavioral manifestations of PD in rats. (ecochildsplay.com)
Wistar rats were gavage-poisoned with 400 ?g AFB1 per kilogram of ration fed for three months, while the AFB1 + ArtinM group received three subcutaneous doses of the lectin (50 ?g per kilogram of animal weight per dose) 45, 60 and 75 days after the start of the experiment. (usp.br)

Targeting these proteins with multivalent glycosides can inhibit biofilm-formation by crosslinking these lectins. (qub.ac.uk)
Targeting undruggable carbohydrate recognition sites through focused fragment library design. (mpg.de)
Targeting the central pocket of the Pseudomonas aeruginosa lectin LecA. (mpg.de)
The diagnostic targeting of a carbohydrate virulence factor from M.Tuberculosis. (mpg.de)

Fungal lectins have attracted great interest due to antitumor, immunomodulatory, and antiproliferative activities. (fapesp.br)

The rat enzyme, however, is heavily glycosylated and binds to wheat-germ lectin-Sepharose 6MB and 5-hydroxytryptamine-agarose, but not to concanavalin A-Sepharose 4B. (wikigenes.org)

In an article published September 2018 edition of the Nature Partner Journal Parkinson's Disease , researchers recreated the disease in rats using low amounts of paraquat and the food protein lectin. (ecochildsplay.com)
It was the combination of lectin and paraquat that led to the creation of the disease in rats. (ecochildsplay.com)
The aim of this study was to investigate the role of ArtinM lectin in AFB1-induced hepatocarcinogenesis in rats. (usp.br)

We investigated whether Norwalk virus or viruslike particles bind specifically to oyster tissues after bioaccumulation or addition to tissue sections. (cdc.gov)

Since noroviruses can attach to carbohydrates of the ABH and Lewis histo-blood group family in humans, we also examined the possibility of a similar specific binding to oyster tissues through related carbohydrates ( 17 , 18 ). (cdc.gov)

We investigated the binding of four lectins to the follicle-associated epithelium (FAE) overlying fixed mouse small intestinal Peyer's patches to identify M-cell-specific surface markers. (nih.gov)
This is the first demonstration of carbohydrates in the glycocalyx of M-cells that are not expressed elsewhere on the FAE surface. (nih.gov)

It introduces mushroom lectins to birds via injection into developing embryos, or through drinking water. (canadianpoultrymag.com)
Lillehoj and scientists at South Korea's Chungnam National University and Rural Resource Development Institute used lectin extracted from Fomitella fraxinea, a wood-rotting mushroom seen mostly on black locust tree stumps. (canadianpoultrymag.com)
This particular lectin is usually prepared under less stringent conditions than are other mushroom compounds that produce a similar effect, making its commercial production more feasible. (canadianpoultrymag.com)

The 10/78 antibody competes with the previously-described 3.2.3 antibody for binding to these CD161 proteins. (bdbiosciences.com)

Specifically, we use computer simulations and formal theory to study problems in the field of carbohydrate and protein chemistry, focusing on structure/function relationships, polymer property prediction, and enzymatic biomass conversion. (bradley.edu)

Organisms1

Chemicals and Drugs25

Phenomena and Processes8

Technology, Industry, Agriculture1

Information Science3

Specificity2

Enzymes6

Receptors3

Galactose-bindin1

Affinity4

Agglutinin3

Animal lectins2

Fucose-specific2

Reagents1

Plant lectins1

Sialic acid4

Sugars3

Soluble1

Glycans8

Conjugation1

Innate Immunity1

Characterization1

Ligands1

Functions2

Specific carbohydrate3

Residues3

Bacterial3

Monosaccharides2

Covalent1

Glycobiology1

Laminin4

Protein-carbohydrate interactions2

Antigen2

Glycoprotein2

Structures1

Proteins found2

Specificities1

Receptor3

Epitopes1

Escherichia1

Collectins1

Interactions2

Recombinant1

Structural2

Moieties1

Glycan2

Domains2

Analyses1

Biological3

Precursor1

Reaction3

Extracellular1

Glycoproteins1

Doses2

Targeting4

Immunomodulatory1

Concanavalin1

Rats3

Tissue1

Tissues1

Surface2

Mushroom3

Antibody1

Chemistry1