Lectin genes carbohydrate sequence. Medical search. Frequent questions

It belongs to a family of proteins called collectins, which consists of collagenous region and a carbohydrate recognition domain (Taylor et al. (springeropen.com)
Galectin-3 belongs to the lectin family of carbohydrate binding proteins. (enquirebio.com)
The amino acid sequences of human and mouse Galectin-3 proteins share 80% homology. (enquirebio.com)
Lectins are a diverse group of proteins, both in terms of structure and activity. (embl.de)
When examined from the viewpoint of the bound sugar, the structural information accumulated so far on lectins and other proteins that are specific to galactose and glucose (or mannose), provides suggestive evidence for distinct ligand-dependent distribution of hydrogen-bond partners in the combining site. (embl.de)
Finally, genes related to structural proteins, immune proteins and protectants were overexpressed or positively selected. (prelekara.sk)
The expression of this lectin is upregulated during inflammation, cell proliferation, cell differentiation and through transactivation by viral proteins. (liveconscience.com)
Proteins are identified by genes that determine how to be produced and which ones to make. (vumc.org)
The glycocalyx is composed of carbohydrates that are part of the cell surface exposed lipids and proteins (including proteoglycans) or part of the extracellular matrix. (uni-greifswald.de)
Ectopic expression of full-length-c-MYC fusion proteins with the respective mutations in LMTK- cells showed severe functional impairment and subcellular mislocalization of all mutant gene products [5]. (uni-greifswald.de)
Similarity searches revealed that 55% of these had hits in protein sequence databases, of which 1,729 had their best hit with proteins from the chromalveolates (E-value ≤ 1e-5). (biomedcentral.com)
Some sequences are similar to those proven to be targets for effective intervention in other protozoan parasites, and include not only proteases, antioxidant enzymes, and heat shock proteins, but also those associated with relict plastids, such as acetyl-CoA carboxylase and methyl erythrithol phosphate pathway components, and those involved in glycan assembly, protein folding/secretion, and parasite-host interactions. (biomedcentral.com)
It provides a very informative, albeit preliminary, glimpse into the expression of genes encoding functionally relevant proteins as potential targets for chemotherapy, and evidence for the presence of a relict plastid. (biomedcentral.com)

LOX-1 is a receptor protein which belongs to the C-type lectin superfamily. (wikipedia.org)
LSECtin (liver and lymph node sinusoidal endothelial cell C-type lectin), also known as C-type lectin superfamily 4, member G (CLEC4G), is a member of subgroup II of the C-type (Ca 2+ -dependent) lectin superfamily (1). (rndsystems.com)

It binds to carbohydrates on the surface of mannose-rich pathogens and mediates clearing by phagocytosis or complement activation (Nepomuceno et al. (springeropen.com)
Binds to GalNAc and GlcNAc carbohydrate moieties (PubMed:12679857). (xenbase.org)
The encoded protein binds specific carbohydrate moieties found on lipids and on the surface of microorganisms. (lsbio.com)
Mouse Embryo Fibroblast Cells with Texas Red-X, Alexa Fluor 488, and DAPI - In this section, a Swiss mouse embryo fibroblast cell is presented that was resident in a culture labeled with the fluorophore Texas Red-X conjugated to wheat germ agglutinin, a fluorescent lectin that selectively binds to sialic acid residues. (fsu.edu)

This domain, which is known as the C-type lectin domain (CTL) or as the carbohydrate-recognition domain (CRD), consists of about 110 to 130 residues. (embl.de)
The enzyme encoded by this gene controls the initiation step of mucin-type O-linked protein glycosylation and transfer of N-acetylgalactosamine to serine and threonine amino acid residues. (nih.gov)
Digestion of carbohydrate residues on the protein with an acetylglucosaminidase plus binding to several lectins indicates that the enzyme is glycosylated. (inra.fr)
The full-length cDNA of Arisaema lobatum agglutinin (ala) was 1078 bp and contained a 774 bp open reading frame encoding a lectin precursor (proproprotein) of 258 amino acid residues with a 23 aa signal peptide. (lookformedical.com)

Moreover, 15 out of 25 genes involved in cellular carbohydrate metabolism were related to trehalose biosynthesis, versus 1 out of 5 in A . longirostris . (prelekara.sk)
Putative positively selected genes involved in chitin metabolism and the immune system (lectin, serine protease and antimicrobial peptide) were enriched in S . leurokolos . (prelekara.sk)

The 45-kDa form was pulled down by lectin concanavalin A (Con A) and Galant husnivalis lectin (GNL), indicating an immature glycan with a high amount of mannose (Man), whereas the 65-kDa form is a mature glycan composed of acetylglucosamine (GlcNAc) and poly-N-acetyllactosame (poly-LacNAc) that was pulled down by wheat germ agglutinin (WGA) and tomato lectin, respectively. (emory.edu)

Using transcriptomics and single cell sequencing we explore differential expression of glycosylation related genes in cancer patients tissues to unravel altered glycosylation signatures, to predict for survival benefit, response to therapy, and glycan induced immune modulation. (immunologyamsterdam.org)
Its extracellular region consists of two N-linked glycosylation sites (aa 73 and 159), a coil‑coil neck domain (aa 96‑136), a C‑type lectin-like domain (CTLD) of the type found in human DC‑SIGN and DC‑SIGN receptor (aa 165‑289), and a C-terminal Ca 2+ -dependent carbohydrate‑recognition domain (C-type CRD) (1). (rndsystems.com)
Using sugar-specific binding lectins, the UT-A3 glycosylation profile was examined. (emory.edu)
Mass spectrometry and lectin arrays are common technologies publicly adopted in glycosylation analysis. (frontiersin.org)

To overcome the host plant defense response, M. phaseolina encodes a significant number of P450s, MFS type membrane transporters, glycosidases, transposases, and secondary metabolites in comparison to all sequenced ascomycete species. (biomedcentral.com)
Subsequently DNase 1 has been isolated from many mammalian species as well as chicken, snake and xenopus and it has been cloned and sequenced from 36 species. (pancreapedia.org)
The predicted amino acid sequence yields a protein mass of 30,200 Da, but on polyacrylamide gel electrophoresis or size exclusion chromatography it has an apparent molecular mass of 32-38 kDa and by isoelectric focusing a PI of about 3.5 - 4.0 depending on species and technique. (pancreapedia.org)
The carbohydrate moieties differ among species but allow purification by lectin chromatography. (pancreapedia.org)
Full-length nucleotide sequences of the genomes of members of all filovirus species have been determined. (ictv.global)
Genomicand genetic relationships among species of Leymus (Poaceae: Triticeae) inferred from 18S-26S ribosomal genes. (degruyter.com)

Its gene is regulated through the cyclic AMP signaling pathway. (wikipedia.org)

The results obtained here indicate that the binding of IgG in NHS to the LOS and LPS samples is probably due to their carbohydrate moieties. (go.jp)
Mediates the endocytosis of plasma glycoproteins to which the terminal sialic acid residue on their complex carbohydrate moieties has been removed. (joplink.net)

Cloning and characterization of an agglutinin gene from Arisaema lobatum. (lookformedical.com)
A novel agglutinin gene was cloned from Arisaema lobatum using SMART RACE-PCR technology. (lookformedical.com)

Mannose binding lectin (MBL) is a liver derived protein which plays an important role in innate immunity. (springeropen.com)
May function in innate immunity through activation of the lectin complement pathway. (xenbase.org)

We investigate how binding of carbohydrates, altered due to tissue inflammation or wound healing processes, control modulation of immune cells through recognition/signaling of glycan binding receptors, such as C-type lectins and Siglecs. (immunologyamsterdam.org)
Lectin-carbohydrate interactions: different folds, common recognition principles. (embl.de)
Lectins can be found in many organisms and are involved in a multitude of cellular processes that depend on specific recognition of complex carbohydrates. (embl.de)
Structural basis of lectin-carbohydrate recognition. (embl.de)
Lectins are responsible for cell surface sugar recognition in bacteria, animals, and plants. (embl.de)
Human mannose-binding protein carbohydrate recognition domain trimerizes through a triple α-helical coiled-coil. (lu.se)
The sequence starts with the binding of 2 or more C1q recognition units to the Fc nonantigen binding part of IgG and IgM molecules. (medscape.com)

Mannose binding lectin gene 2 ( MBL2 ) polymorphisms are reported to be associated with various diseases. (springeropen.com)
Mannose binding lectin (MBL) is a liver derived acute phase protein. (springeropen.com)
Furthermore, differential binding was observed also to known endogenous ligands C1q, mannose-binding lectin, and secretory IgA. (researchgate.net)

Genomic sequence of ala was also cloned using genomic walker technology, and it was found to contain three putative TATA boxes and eight possible CAAT boxes in the 5'-flanking region. (lookformedical.com)
No intron was found within the region of genomic sequence. (lookformedical.com)

Marine viruses impact global biogeochemical cycles via their influence on host community structure and function, yet our understanding of viral ecology is constrained by limitations in host culturing and a lack of reference genomes and 'universal' gene markers to facilitate community surveys. (peerj.com)
The 'VirION' (Viral, long-read metagenomics via MinION sequencing) approach was first validated using mock communities where it was found to be as relatively quantitative as short-read methods and provided significant improvements in recovery of viral genomes. (peerj.com)
The PDMs were linked to 81 gene clusters in genomes of known lignocellulose degraders, including previously described clusters of lignocellulolytic genes. (biomedcentral.com)
Filovirus genomes are characterized by the gene order: 3′-NP-VP35-VP40-GP-VP30-VP24-L-5′ (Figure 2). (ictv.global)
The extragenic sequences at the extreme 3′ (leader) and 5′ (trailer) ends of the genomes are conserved. (ictv.global)

Galactose binding lectin domain. (unl.edu)

When applied to human macrophage-derived foam cells in vitro, the dietary supplement berberine inhibits the expression of the ORL1 gene in response to oxidized low-density lipoprotein cholesterol, but this has not yet been demonstrated in a living animal or human. (wikipedia.org)
Out of these, 4 SNPs from 3′ UTR were found to play role in miRNA binding, 7 SNPs from 5′ near and intronic region were predicted to involve in transcription factor binding and expression of MBL2 gene. (springeropen.com)
Western blot analysis showed a weak UT-B band of 40 kDa in some tumors, consistent with UT-B1 gene expression detected by RT-PCR. (emory.edu)
In conclusion, SLC14A1 gene and UT-B protein expression are significantly changed in bladder cancers. (emory.edu)
The cloning of the ala gene not only provides a basis for further investigation of its structure, expression and regulation mechanism, but also enables us to test its potential role in controlling pests and fungal diseases by transferring the gene into plants in the future. (lookformedical.com)
miRNAs are short single-stranded RNA molecules that function as grasp regulators of gene expression by post-transcriptional modifications of target mRNAs [15]. (liveconscience.com)
The pattern of regulation of gene expression is usually sequence-specific. (liveconscience.com)
Lectin like protein whose expression is induced upon treatment with chitin oligomers. (riken.jp)

SFTPA1 / Surfactant Protein A is a lung surfactant protein that is a member of a subfamily of C-type lectins called collectins. (lsbio.com)

HBGA are complex carbohydrates distinguished by different monosaccharides added to a precursor oligosaccharide by fucosyltransferase enzymes ( 2 3, 24 ). (cdc.gov)

Purification and characterization of a lectin from Arisaema tortuosum Schott having in-vitro anticancer activity against human cancer cell lines. (lookformedical.com)

A strikingly distinct set of carbohydrate esterases (CE) are present in M. phaseolina , with the CE9 and CE10 families remarkably higher than any other fungi. (biomedcentral.com)

Productive folding of the protein moiety is facilitated during this period by the calnexin/calreticulin cycle in mammalian cells, which relies on G1M9-specific lectin-type chaperones (calnexin and calreticulin) associated with the oxidoreductase ERp57, and UDP-glucose:glycoprotein glucosyltransferases 1 and 2, which are capable of re-adding glucose to M9 if the protein moiety is not yet folded. (elifesciences.org)
Due to a Cterminal carbohydrate binding site, Galectin 3 is capable of binding IgE and mammalian cell surfaces only when homodimerized or homooligomerized. (liveconscience.com)

As a broad host range pathogen, M. phaseolina possesses a large number of pathogen-host interaction genes including those for adhesion, signal transduction, cell wall breakdown, purine biosynthesis, and potent mycotoxin patulin. (biomedcentral.com)

Mutations of the OLR1 gene have been associated with atherosclerosis, risk of myocardial infarction, and may modify the risk of Alzheimer's disease. (wikipedia.org)
Recently, gene analyses demonstrate that SLC14A1 (UT-B) gene mutations are associated with bladder cancer, suggesting that urea transporter UT-B may play an important role in bladder carcinogenesis. (emory.edu)
Mutations in this gene are associated with idiopathic pulmonary fibrosis. (lsbio.com)
Blue squares indicate phenotypes directly attributed to mutations/alleles of this gene. (jax.org)

It is made up of a short N-terminal signal peptide and the mature enzyme, which contains 259 - 262 amino acids (260 for human) and, in most cases, a small amount of carbohydrate (6,11,24,42). (pancreapedia.org)
The resulting sequences were submitted to functional annotation against non-redundant NCBI database and Database of Anuran Defense Peptide. (biomedcentral.com)

In this study, we screened candidate genes that might be involved in niche separation and microhabitat adaptation through comparative transcriptomics. (prelekara.sk)
PDMs represent a new resource of protein families and candidate genes implicated in microbial plant biomass degradation. (biomedcentral.com)

This gene encodes GalNAc transferase 7, a member of the GalNAc-transferase family. (nih.gov)
Structural basis of carbohydrate transfer activity of UDP-GalNAc: Polypeptide N-acetylgalactosaminyltransferase 7. (nih.gov)

1998 ). The MBL2 gene is composed of 7461 bases and lies between the regions 52765380 to 52772841 bp of chromosome no. 10 (NCBI reference sequence number NC_000010.11). (springeropen.com)

We sequenced and assembled ~49 Mb into 15 super-scaffolds covering 92.83% of the M. phaseolina genome. (biomedcentral.com)
Nautilus shows a compact, minimalist genome with few encoding genes and slow evolutionary rates in both non-coding and coding regions among known cephalopods. (nature.com)
In this study, we sequenced the complete genome of Nautilus pompilius in the hope of providing a critical reference for the evolution of cephalopods. (nature.com)
The EST analysis reported herein, together with the recently completed sequence of the P. marinus genome and the development of transfection methodology, should result in improved intervention strategies against dermo disease. (biomedcentral.com)

We investigate glycan binding receptors such as C-type lectins and Siglecs on innate myeloid, macrophages and DC for targeting purposes to guide glycosylated antigens binding and uptake that influence antigen presentation, T cell differentiation and immune cell signaling. (immunologyamsterdam.org)

2006 ). Mice have two homologous genes of MBL i.e. (springeropen.com)

Oxidized low-density lipoprotein receptor 1 (Ox-LDL receptor 1) also known as lectin-type oxidized LDL receptor 1 (LOX-1) is a protein that in humans is encoded by the OLR1 gene. (wikipedia.org)
Rat liver asialoglycoprotein receptor lacks a cleavable NH2-terminal signal sequence. (joplink.net)

Short-read viral metagenomic studies have provided clues to viral function and first estimates of global viral gene abundance and distribution, but their assemblies are confounded by populations with high levels of strain evenness and nucleotide diversity (microdiversity), limiting assembly of some of the most abundant viruses on Earth. (peerj.com)

Interacting selectively and non-covalently with any carbohydrate, which includes monosaccharides, oligosaccharides and polysaccharides as well as substances derived from monosaccharides by reduction of the carbonyl group (alditols), by oxidation of one or more hydroxy groups to afford the corresponding aldehydes, ketones, or carboxylic acids, or by replacement of one or more hydroxy group(s) by a hydrogen atom. (unl.edu)

The structure of the human and mouse gene contains eight coding exons and is located on chromosome 16 (28,41). (pancreapedia.org)

5. Smith, M. J. and Koch, G. L. (1989) Multiple zones in the sequence of calreticulin (CRP55, calregulin, HACBP), a major calcium binding ER/SR protein. (bomiprot.org)

Thus, findings of the present study indicated 12 SNPs of MBL2 gene to be functionally important. (springeropen.com)

The complement system functions as an interactive sequence, with one reaction leading to another in the form of a cascade. (medscape.com)
Depending on the nature of complement activators, the classic pathway, the alternative pathway, or the more recently discovered lectin pathway is activated predominantly to produce C3 convertase. (medscape.com)

This enzyme is a type II transmembrane protein and shares common sequence motifs with other family members. (nih.gov)
A lectin with in-vitro anticancer activity against established human cancer cell lines has been purified by affinity chromatography on asialofetuin-linked amino activated silica beads from the tubers of Arisaema tortuosum, popularly known as Himalayan Cobra lily, a monocot plant from the family Araceae. (lookformedical.com)
Southern blot analysis indicated that the ala belonged to a multi-copy gene family. (lookformedical.com)
A tuber lectin from Arisaema jacquemontii Blume belonging to family Araceae was purified by employing a single step affinity chromatography using column of asialofetuin-linked amino activated silica beads and the bound lectin was eluted with 100 mM glycine-HCl buffer pH 2.5. (lookformedical.com)

The present study was carried out to identify and prioritize the SNPs of MBL2 gene for further genotyping and functional studies. (springeropen.com)
MBL1 and MBL2 , but in human the first of them is pseudogene leaving one functional gene MBL2 (Guo et al. (springeropen.com)
On average, 70% of the families of each PDM were found to map to gene clusters in known degraders, which served as an additional confirmation of their functional relationships. (biomedcentral.com)
Persons who lack a functional FUT2 gene cannot generate ABH antigens in secretions and, thus, are termed nonsecretors ( 23 , 24 ). (cdc.gov)

However, as reported for other monocot lectins, ATL gave multiple bands in isoelectric focusing and Native PAGE, pH 8.3. (lookformedical.com)

Such features also challenge assembly across genomic islands containing niche-defining genes that drive ecological speciation. (peerj.com)

Poaceae) based on a single-copy nuclear gene encoding plastid acetyl-CoA carboxylase. (degruyter.com)

The function of a protein is determined by the number and sequence of amino acids, which controls the three-dimensional structure of the resulting folded polypeptide. (frontiersin.org)
Each codon corresponds to a specific amino acid, and the sequence of codons determines the sequence of amino acids in the protein. (vumc.org)

The detection level accomplished in this study should help not only to further characterize anti-LOS antibodies in blood and body fluids but also to analyze carbohydrate structures that are recognized by them. (go.jp)

Lectin array technology is based on the lectin-glycan affinity ( 6 ), which is not suitable for glycoproteomic analysis. (frontiersin.org)

Human LSECtin shares 64% aa sequence identity with mouse LSECtin. (rndsystems.com)
The lectin was found to inhibit in vitro proliferation of human cancer cell lines HT29, SiHa and OVCAR-5. (lookformedical.com)
The purified lectin agglutinated erythrocytes from rabbit but not from human. (lookformedical.com)
A major finding from human NV volunteer studies is that persons with strains containing 2 mutated alleles of the α(1,2) fucosyltransferase ( FUT2 ) gene were resistant to NV infection ( 16 - 20 ). (cdc.gov)

Anatomy1

Organisms6

Diseases1

Chemicals and Drugs22

Analytical, Diagnostic and Therapeutic Techniques and Equipment1

Phenomena and Processes15

Technology, Industry, Agriculture1

Information Science4

Health Care1

Proteins13

Superfamily2

Binds4

Residues4

Metabolism2

Wheat germ agglutinin1

Glycosylation4

Species6

Signaling pathway1

Moieties2

Agglutinin2

Innate immunity2

Recognition7

Mannose3

Genomic sequence2

Genomes5

Galactose1

Expression8

Collectins1

Precursor1

Purification1

Esterases1

Mammalian2

Signal transduction1

Mutations4

Peptide2

Candidate genes2

GalNAc2

NCBI1

Genome4

Cell differentiation1

Homologous1

Receptor2

Nucleotide1

Monosaccharides1

Chromosome1

Calreticulin1

Functionally1

Complement2

Family4

Functional4

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Ecological1

Plastid1

Amino acids2

Structures1

Glycan1

Human4