Latter formation biofilms. Medical search. Frequent questions

A biofilm comprises any syntrophic consortium of microorganisms in which cells stick to each other and often also to a surface. (wikipedia.org)
Biofilms can attach to a surface such as a tooth or rock, and may include a single species or a diverse group of microorganisms. (wikipedia.org)
The formation of a biofilm begins with the attachment of free-floating microorganisms to a surface. (wikipedia.org)
Biofilms (so called because macroscopically they do look like a thin layer of slime) have a distinct architecture, consisting of tower- and mushroom-shaped microcolonies encased in a hydrated matrix of exopolymeric substances, polysaccharides and proteins that are produced by the resident microorganisms. (biomedcentral.com)
Disinfection methods currently used in water systems have been shown to be ineffective against legionella over the long-term, allowing recolonization by the biofilm-protected microorganisms. (frontiersin.org)
Groups of microorganisms that stick to each other to form colonies in a slimy layer covering living or non-living surfaces, biofilms can slow or halt healing. (equusmagazine.com)
Biofilms are complex and structured communities of microorganisms that are found in a variety of natural and man-made environments. (scientiaen.com)
Biofilms are composed of populations of microorganisms that are embedded in a matrix of EPS. (scientiaen.com)
Biofilms are three-dimensional structures consisting of various microbial populations attached to a surface by extracellular polymeric substances (EPS) produced by these microorganisms [ 1 ]. (biomedcentral.com)
Biofilms are microorganisms that cling to a surface in a very narrow space and form a protective layer, the so-called matrix. (foodsafetynews.com)
The devices become colonized by the microorganisms that form a biofilm of cells, the detachment of which can result in septicemia ( 2 - 5 , 8 , 9 , 14 , 15 ). (cdc.gov)
Biofilms are universal, complex, interdependent communities of surface-associated microorganisms. (cdc.gov)
Most microorganisms grow in structured biofilms rather than individually in suspensions and while in this environment may display altered phenotypes ( 2 ) . (cdc.gov)
Biofilm formation begins when microorganisms in water come in contact with a surface and attach to it. (corzan.com)
Completely dry surfaces do not contain the microorganisms needed for biofilm formation. (corzan.com)
These harmful bacteria or microorganisms can harbor in the accumulated biofilm and deposit into the flow. (corzan.com)
For biofilm to grow, especially if the water is not entirely stagnant, microorganisms need something to attach to. (corzan.com)
Suppression of bacterial biofilms formation, toxic effects on microorganisms, and mutagenesis enhancement by glyphosate can lead to negative consequences for natural microbiomes . (bvsalud.org)

Bacterial ability to form biofilms was verified using a crystal violet colorimetric assay and testing cell viability by real-time quantitative PCR and Plate Count assay. (frontiersin.org)
Aggregatibacter actinomycetemcomitans has been strongly associated with localized aggressive periodontitis via its ability to form biofilms in the subgingival cavity [ 12 ]. (biomedcentral.com)
Thus, the aim of this research was to determine whether yeast present in CVC colonizations previously exposed to cell- wall targeted antibacterials benefit from a reduction in susceptibility to fluconazole and voriconazole, facilitating their ability to form biofilms. (bvsalud.org)
None of the antibacterials exerted a significant effect on the in vitro susceptibility of the yeasts to the antifungal agents or on their ability to form biofilms. (bvsalud.org)

They found that at low concentrations, the compound, DAPG (the acronym stands for 2,4-diacetylphloroglucinol), produced by the bacterium Pseudomonas protegens, did not kill the experimental target bacterium, Bacillus subtilis, but merely prevented it from forming biofilms. (sciencedaily.com)
In this study, well-defined tannins present in multiple plant materials (tannic acid (TA) and epigallocathecin gallate (EGCG)) and undefined cranberry powder (CP) were found to block swarming motility and enhance biofilm formation and resistance to tobramycin in Pseudomonas aeruginosa. (cranberryinstitute.org)
The carboxybetaine precursors undergo rapid, self-catalysed hydrolysis at the water/material interface and provide critical anti-fouling properties that lead to undetectable bacterial attachment and zero biofilm formation after six months of constant exposure to Pseudomonas aeruginosa and Staphylococcus epidermidis under the static condition in a nutrient-rich medium. (european-coatings.com)
In this study, the anti-biofilm activity of previously fabricated polyamino-phenolic ligands and polyamidoamine dendrimers was investigated against legionella mono-species and multi-species biofilms formed by L. pneumophila in association with other bacteria that can be found in tap water ( Aeromonas hydrophila , Pseudomonas aeruginosa , Escherichia coli , Klebsiella pneumoniae ). (frontiersin.org)
Most often we were able to assign the biofilm bacteria of the genera Brochothrix, Pseudomonas and Psychrobacter. (foodsafetynews.com)

Any drug impairing crucial processes for bacterial life will inevitably lead to the development of drug-resistant strains, whereas the inhibition of biofilm formation might prevent the onset of bacterial resistance. (intechopen.com)
In this section, we will focus on proteins involved in biofilm formation as useful targets for the development of new drugs that can effectively and specifically impair biofilm formation with slight effects on cell survival, thus avoiding the generation of drug-resistant strains. (intechopen.com)
Two newly identified genetic loci, dltABCD and phoPR, were selected for deletion analysis and both ΔdltABCD and ΔphoPR bacterial strains displayed biofilm formation defects in the PVC microtiter plate assay, confirming these loci contribute to biofilm formation by L. monocytogenes. (harvard.edu)
The viscoelastic properties of this biofilm contributes to its ability to evade phagocytic activities, to reduce the effectiveness of antibiotics, to develop antibiotic resistant strains, and to persist or reoccur in CF patients. (aiche.org)
This mutant had obvious differences in colony morphology (smaller plaque, edge smooth and round) and defects in biofilm formation in contrast with the wild-type (WT) strains. (biomedcentral.com)
In one ICU out of ten, the molecular characteristics, antimicrobial susceptibility profiles and biofilm production of the strains responsible for S. aureus bacteremia were studied. (hal.science)
Under static conditions and without exposure to glucose, biofilm production was rare (9.4% of the strains, without any CC398). (hal.science)
Methods and Results: Biofilm formation on plastic and cFn-coated surfaces by the wild-type M1 and M41 strains, and their isogenid sc/1-inactivated mutants, we compared spectrophotometrically following crystal violet staining. (cdc.gov)
Both WT strains formed significantly richer biofilm on cFn than on plastic, indicating that cell anchoring with ECM strengthens the overall biofilm structure. (cdc.gov)
Biofilm formed on nECM was comparable to that formed on commercial cFn and there was a statistically significant decrease in biofilm formed by both scl1 mutants as compared with the WT strains. (cdc.gov)
Bravo and colleagues [ 1 ] investigated the ability of Acinetobacter pittii strains to form biofilms after long-term desiccation if they were fed with nutrient media. (medscape.com)
Despite the reduction in culturability over time, all strains that recovered from starvation on solid-surface experiments retained their capacity to form biofilms after rehydration, an addition of nutrients, and changing temperature. (medscape.com)

Bacteria can be found in planktonic form or in specific conditions, as sessile aggregates on both biotic and abiotic surfaces originating complex structures known as biofilm. (intechopen.com)
Bacteria can adhere and grow on multiple surfaces and persist within biofilms in food processing plants, providing resistance to sanitizers and other antimicrobial agents. (harvard.edu)
The ability of Streptococcus mutans to adhere to oral surfaces and form biofilm is a key step in the tooth decay process. (cranberryinstitute.org)
Biofilms may form on living (biotic) or non-living (abiotic) surfaces and can be prevalent in natural, industrial, and hospital settings. (wikipedia.org)
Bacteria predominantly grow inside multicellular communities attached to solid surfaces and enclosed in a self-produced polymeric matrix, called biofilms [ 1 ]. (biomedcentral.com)
It was shown for example that an incomplete LPS fraction does not affect biofilm formation capacity of Salmonella on hydrophobic gallstone surfaces, but highly reduces its biofilm capacity on hydrophilic glass surfaces [ 7 ]. (biomedcentral.com)
In general, micro-organisms have the tendency to attach to surfaces and develop biofilms. (maritech.org)
In the context of biofilm formation, surface tension plays a key role in bacterial attachment to surfaces. (scientiaen.com)
Streptococcus mutans , a representative bacteria of tooth decay [ 10 ], forms plaques (biofilm) on tooth surfaces and produces lactate from sugars such as sucrose, which demineralizes teeth and causes dental caries. (biomedcentral.com)
Scientists from the Feed and Food Quality, Safety and Innovation (FFoQSI) Competence Center examined 11 rooms in November 2018 within the plant and took 108 samples - 47 food contact surfaces before operation and 61 surfaces not directly in contact with food during daily operation - for the presence of biofilms. (foodsafetynews.com)
Five biofilms were detected on non-food contact surfaces like drains and water hoses resulting in 9.3 percent of the sites being classed as biofilm positive. (foodsafetynews.com)
If a biofilm is now in the water hose, freshly cleaned areas, including food contact surfaces, can be contaminated again. (foodsafetynews.com)
Most manifestations of candidiasis are in fact associated with the formation of Candida biofilms on surfaces, and this phenotype is associated with infection at both the mucosal and systemic sites ( 8 ) . (cdc.gov)
Biofilms are sessile microbial communities growing on surfaces, frequently embedded in a matrix of extracellular polymeric substances (also known as slime). (medscape.com)
These findings are consistent with a tendency of other Acinetobacter species to survive on dry surfaces, form biofilms, and contribute to nosocomial outbreaks. (medscape.com)

A biofilm is any group of microbes that stick together on a surface. (sciencedaily.com)
Microbes form a biofilm in response to a number of different factors, [9] which may include cellular recognition of specific or non-specific attachment sites on a surface, nutritional cues, or in some cases, by exposure of planktonic cells to sub-inhibitory concentrations of antibiotics . (wikipedia.org)
Furthermore, vinegar rinsing will destruct mature (24-h) biofilms, and significantly reduce the viability of planktonic microbes in saliva, thereby decreasing biofilm formation. (biomedcentral.com)
The essential requirements for biofilm growth are the microbes themselves and a substrate. (maritech.org)
Biofilms, which are complex layered communities of sulfur-consuming microbes, increase the rate of cave formation, but may also shed light on other biofilms, including those that grow on teeth and those that corrode steel ships hulls, according to a team of geologists. (karstworlds.com)
Cave biofilms are simpler than the microbes that occur in soils where there can be hundreds of thousands of species," says Dr. Jennifer L. Macalady, assistant professor of geosciences, Penn State. (karstworlds.com)
Microbes in the biofilms use the sulfur for energy and produce sulfuric acid, a very strong acid. (karstworlds.com)
In dental biofilms, the microbes on the teeth are the ones that produce the acids that cause cavities, while the ones on the top create the right conditions for the acid-producing microbes to survive. (karstworlds.com)
And, microbes found in biofilm that are not hazardous can still negatively influence the taste, color and odor for certain fluids, especially water. (corzan.com)

Taken together, the phenotypic and genotypic analysis of the regulation of alginate may further lead to treatments to prevent biofilm formation and subsequent CF host colonization with P. aeruginosa . (marshall.edu)

Historically, multicellular bacterial communities, known as biofilms, have been thought to be held together solely by a self-produced extracellular matrix. (nature.com)
Biofilms are multicellular communities that were so far thought to be held together solely by a self-produced organic extracellular matrix. (nature.com)
Hyperthermophilic archaeon Pyrobaculum calidifontis produce bundling pili which are homologous to the bacterial TasA filaments, a major component of the extracellular matrix in bacterial biofilms, which contribute to biofilm stability. (wikipedia.org)

This study correlates the activity of the quorum sensing inhibitors with viscoelastic changes in the P. aeruginosa biofilm on the air-medium interface. (aiche.org)
The aims of this study were to investigate the prevalence of integrons and biofilm formation among P. aeruginosa and A. baumannii isolates collected from ICU and non-ICU inpatients. (magiran.com)
Furthermore, 23% of the A. baumannii and 12% of the P. aeruginosa isolates showed strong biofilm activity. (magiran.com)
The binding of the positively charged aminoglycosides to the negatively charged biofilm matrix polymers of P. aeruginosa will delay the penetration of aminoglycosides, [ 13 ] while the penetration of fluoroquinolones occurs immediately and without delay. (medscape.com)
the effectiveness of fluoroquinolones on biofilm-growing P. aeruginosa is greater when compared with β-lactams, while both fluoroquinolones and β-lactams are less effective against biofilm-growing P. aeruginosa compared to planktonic cells. (medscape.com)
While free-floating planktonic bacteria can be properly cleared from the CF lung, P. aeruginosa , along with alginate production, establishes an infection in the form of a biofilm which supports its survival in nature and in vivo . (marshall.edu)
Unrelated phenotypic differences in biofilm formation and lung colonization as well as variations in genome size, all within different sources of P. aeruginosa were discovered and could lead to variations in morbidity and mortality related to P. aeruginosa infections in CF. A key factor in alginate regulation is AlgU/AlgT, a sigma factor that also impacts the expression of many other involved proteins. (marshall.edu)
P. aeruginosa small colony variants (SCVs) which prefer the biofilm mode of growth, thrive in harsh environmental conditions and are found in the chronically infected CF lung, were generated in the laboratory for further study. (marshall.edu)

The identification of mutants bearing insertions within several flagellar motility genes previously known to be required for the initial stages of biofilm formation validated the ability of the mutagenesis screen to identify L. monocytogenes biofilm-defective mutants. (harvard.edu)

While Macrophen and Double Macrophen were the most active substances among polyamino-phenolic ligands, dendrimers were overall twofold more effective than all other compounds with a reduction up to 85 and 73% of legionella and multi-species biofilms, respectively. (frontiersin.org)
This clearly shows that these are multi-species biofilms, so different bacteria colonize the same biofilm. (foodsafetynews.com)

In contrast, gallic acid (GA) did not completely block swarming motility and did not affect biofilm formation or tobramycin resistance. (cranberryinstitute.org)

The findings demonstrated that the LAB-CFS treatment considerably slowed Staphylococcus aureus ( S. aureus ) growth and prevented it from forming biofilms. (frontiersin.org)
[ 14 ] The penetration of oxacillin and cefotaxime (β-lactams), and vancomycin and teicoplanin (glycopeptides) is significantly reduced through Staphylococcus aureus biofilms, whereas that of amikacin (aminoglycoside), and rifampicin and ciprofloxacin (fluoroquinolones) was unaffected. (medscape.com)

The microbial community is also a natural source of metabolites and has the potential to be used to create antimicrobial and anti-biofilm agents. (frontiersin.org)
The concentration of the chemicals tested as anti-biofilm agents was chosen based on cytotoxicity assays: the highest non-cytotoxic chemical concentration was used for biofilm inhibition assays, with dendrimer concentration 10-fold higher than polyamino-phenolic ligands. (frontiersin.org)

The virulence factors of Candida albicans are germ tube and biofilm formation, adherence to host tissues, and production of hydrolytic enzymes. (hindawi.com)
Effect of a berry polyphenolic fraction on biofilm formation, adherence properties and gene expression of Streptococcus mutans and its biocompatibility with oral epithelial cells. (cranberryinstitute.org)
Upon optimization of biofilm formation by the test isolates, Adherence test medium (ATM) was found to be the best medium for biofilm formation by both S. enterica serovars Typhi and Paratyphi. (banglajol.info)
The impact of vinegar on the initial microflora adherence to enamel and the formation of biofilm in situ have not yet been systematically analyzed. (biomedcentral.com)

In man-made water systems microbial biofilms increase the resistance of legionella to disinfection, posing a significant threat to public health. (frontiersin.org)
These data suggest that the studied compounds, especially dendrimers, could be considered as novel molecules in the design of research projects aimed at the development of efficacious anti-biofilm disinfection treatments of water systems in order to minimize legionellosis outbreaks. (frontiersin.org)
Seven of the biofilm-positive samples were taken during work and three after cleaning and disinfection, including one at a screw conveyor. (foodsafetynews.com)
To thwart biofilm formation, intensive disinfection is performed to the piping system at regular intervals. (corzan.com)
Three recent articles [ 1 , 2 , 3 ] highlight how our understanding of bacterial persistence and recent advances in disinfection modalities may provide a means for control of biofilms and nosocomial infections. (medscape.com)

These data support the theory that nutritional conditions can elicit an inverse relationship between swarming motility and biofilm formation capacities. (cranberryinstitute.org)

The opportunistic human fungal pathogen Candida albicans relies on cell morphological transitions to develop biofilm and invade the host. (lu.se)
Many such manifestations of infections associated with the formation of Candida biofilms include those occurring on devices such as indwelling intravascular catheters. (cdc.gov)
Biofilm-associated Candida show uniform resistance to a wide spectrum of the currently available conventional antifungal agents, which implies that antimicrobial drugs that specifically target biofilm-associated infections are needed. (cdc.gov)
The novel classes of antifungal agents, the lipid formulation of amphotericins, and the echinocandins have demonstrated unique antifungal activity against the resistant Candida biofilms, providing a breakthrough in the treatment of life-threatening invasive systemic mycoses. (cdc.gov)
Candida dubliniensis shares with C. albicans many virulence factors, such as germ tube formation, exoenzyme production, and phenotypic switching ( 10 ) . (cdc.gov)

However, influenza A virus (IAV) infection releases virulent pneumococci from biofilms in vitro and in vivo. (lu.se)

Therefore, inhibiting the growth of oral pathogens, as well as effectively preventing biofilm formation contribute to reducing these risks. (biomedcentral.com)
Biofilms may include spoilage agents and food pathogens such as Listeria, E. coli and Salmonella. (foodsafetynews.com)

That significant fractions of the bacterial genome could be involved in, or affected during, biofilm formation was shown in Escherichia coli in a genome-wide screen using random chromosomal insertions of a lacZ reporter gene fusion construct [ 5 ]. (biomedcentral.com)
In this study, we established RAINBOW-seq and profiled the transcriptome of Escherichia coli biofilm communities with high spatial resolution and high gene coverage. (nature.com)
Toxicity and mutagenicity, as well as changes in the bacterial biofilm biomass , physiological activity, and the number of living cells in its composition in the presence of glyphosate were assessed using the Escherichia coli model. (bvsalud.org)

Kober-Rychli added further research is needed in the prevention, rapid detection and control of biofilms in the food sector. (foodsafetynews.com)

Because of the protected environment, bacteria within biofilms are less sensitive to environmental stresses, including disinfectants and antibiotics, and are as a consequence very difficult to eradicate [ 3 ]. (biomedcentral.com)

More than 65 percent of hospital-acquired infections manifest as biofilms. (sciencedaily.com)
In fact, it was shown that the majority of all bacterial infections are related to biofilm growth, stressing the importance of this life style [ 2 ]. (biomedcentral.com)
The capacity of S. aureus to build biofilms is correlated with the severity of infections ( 4 ). (frontiersin.org)
Bacterial communities that are attached to a surface, so-called biofilms, and their inherent resistance to antimicrobial agents are a cause of many persistent and chronic bacterial infections. (biomedcentral.com)
Formation of these sessile communities and their inherent resistance to antimicrobial agents are at the root of many persistent and chronic bacterial infections. (biomedcentral.com)
Biofilms have been shown to colonize a wide variety of medical devices and to be associated with several human diseases, such as native valve endocarditis, burn wound infections, chronic otitis media with effusion and cystic fibrosis. (biomedcentral.com)
Recent advances in our understanding of the genetic and molecular basis of bacterial community behavior point to therapeutic targets that may provide a means for the control of biofilm infections. (biomedcentral.com)
Biofilms have a significant impact on human health, as they are known to cause chronic infections and contribute to the spread of antibiotic-resistant bacteria. (scientiaen.com)
Biofilm-associated infections are difficult to treat using conventional antibiotics, as the EPS matrix provides protection and a barrier to antibiotic penetration. (scientiaen.com)
One of the most intriguing and clinically relevant features of microbial biofilms is their significantly higher antibiotic resistance relative to their free-floating counterparts, which generates serious consequences for therapy of biofilm-associated infections. (medscape.com)
Fungal biofilm-associated infections are frequently refractory to conventional therapy because of resistance to antimicrobial agents. (cdc.gov)
The use of drugs effective in combating biofilm-associated infections could lead to major developments in the treatment of fungal implant infections. (cdc.gov)

The second maturation phase is reached at stage 4 with fully mature biofilms, as indicated by the complex biofilm architecture. (biomedcentral.com)

In this study, we evaluated the ability of two types of biosurfactants (rhamnolipids and surfactin) to inhibit growth and biofilm formation ability of oral pathogenic bacteria such as Aggregatibacter actinomycetemcomitans , Streptococcus mutans , and Streptococcus sanguinis . (biomedcentral.com)
Streptococcus sanguinis is another plaque-forming strain that has been reported to cause infective endocarditis by forming biofilms on the endocardium and heart valves [ 11 ]. (biomedcentral.com)

Biofilms are notoriously resistant to antibiotics. (sciencedaily.com)
The disruption of biofilm formation would have a beneficial impact on the treatment of diseased CF patient as it will make the infection more susceptible to antibiotics and host phagocytosis. (aiche.org)
When investigating potential applications of anti-swarming materials it is important to consider whether the lack of swarming corresponds with an enhanced sessile biofilm lifestyle and resistance to antibiotics. (cranberryinstitute.org)
Class 1 integron-positive isolates were resistant to three classes of antibiotics and predominantly observed in specimens collected from ICU patients showing strong biofilm. (magiran.com)
[13] The biofilm bacteria can share nutrients and are sheltered from harmful factors in the environment, such as desiccation, antibiotics, and a host body's immune system. (wikipedia.org)
The first 'medical' biofilm was described in 1982 in a pacemaker patient, where biofilm was detected on the pacemaker and kept seeding bacteria into the bloodstream of the patient, and antibiotics didn't help, as biofilms are extremely tolerant towards antibiotics," says Jørgensen. (equusmagazine.com)
The MIC of antibiotics to biofilm-growing bacteria may be up to 1000-fold higher than that of planktonic bacteria. (medscape.com)
The biofilm matrix can act as a barrier to delay the diffusion of antibiotics into biofilms [ 11 ] because antibiotics may either react chemically with biofilm matrix components or attach to anionic polysaccharides. (medscape.com)
[ 12 ] Antibiotics have been shown to readily penetrate biofilms in some cases, but poorly in others depending on particular antibiotics and biofilms. (medscape.com)
thereby, the slow growth rates of biofilm-growing cells will render them less susceptible to antibiotics. (medscape.com)

Oral biofilm has been accepted to be the main reason of oral diseases, such as caries and periodontitis which are caused by dysbiosis and imbalance of the biofilm's composition [ 2 ]. (biomedcentral.com)
Although vinegar has such antibacterial effects, there is few research of vinegar applied to the oral biofilm. (biomedcentral.com)
Health-care-regimens were maintained for 1-week, after which wires were removed and oral biofilm was collected. (rug.nl)
Conclusion: Oral biofilm left-behind after powered brushing in-vivo enabled better penetration of antibacterials than after manual brushing. (rug.nl)
Clinical significance: Mechanical removal of oral biofilm is important for prevention of dental pathologies, but biofilm is always left-behind, such as in fissures, buccal pits, interproximal areas and gingival margins and around orthodontic appliances. (rug.nl)
Health-care-regimens were maintained for 1-week, after which wires were removed and oral biofilm was collected.Results: When powered toothbrushing was applied, slightly less bacteria were collected than after manual brushing, regardless whether an antibacterial-regimen was used or not. (rug.nl)

Of the 28 Salmonella Typhi isolates, 17 (61%) were very strong biofilm producers, 8 (29%) were strong biofilm producers and 3 (11%) were moderate biofilm producers. (banglajol.info)
On the other hand, out of 13 S . Paratyphi, 9 (69%) were very strong biofilm producers, 3 (23%) were strong biofilm formers and 1 (8%) was a moderate biofilm producer. (banglajol.info)

In the present study the ability of clinical isolates of Salmonella enterica serovars Typhi (n = 30) and Paratyphi A (n = 11) to form biofilm on abiotic surface was investigated. (banglajol.info)
Biofilm formation is an important survival strategy of Salmonella in all environments. (biomedcentral.com)
In order to unravel how this regulator impinges on Salmonella biofilm formation, we aimed at elucidating the S . Typhimurium FabR regulon. (biomedcentral.com)
Overall, our results point at the importance of FabR and UFA biosynthesis in Salmonella biofilm formation and their role as potential targets for biofilm inhibitory strategies. (biomedcentral.com)
Furthermore, the presence of flagella and fatty acid containing structures such as lipopolysaccharides was shown to be important in Salmonella biofilms [ 7 ]. (biomedcentral.com)

SEM results showed concentration dependent reduction in biofilm and hyphae formation. (hindawi.com)
These compounds, benzyl α-l-fucopyranoside and benzyl β-d-xylopyranoside, inhibit the hyphae formation and adhesion of C. albicans to a polystyrene surface, resulting in a reduced biofilm formation. (lu.se)

Researchers at the Okinawa Institute of Science and Technology in Japan decided to look more closely at the functionality of biofilms, which led to the creation of a unique instrument for studying how biofilms form and for experimenting how drugs can influence them. (medgadget.com)
One of its main virulence factors is its ability to form viscous, alginate-encased biofilms within the pulmonary mucus. (aiche.org)
The present study raises concern from a public health point of view because the ability of the clinical isolates to form biofilm would indicate their ability of being transmitted from abiotic surface to uninfected host giving rise to disease. (banglajol.info)
[8] Biofilms can form on the teeth of most animals as dental plaque , where they may cause tooth decay and gum disease . (wikipedia.org)
A biofilm usually begins to form when a free-swimming bacterium attaches to a surface. (wikipedia.org)
Hydrophobicity can also affect the ability of bacteria to form biofilms. (wikipedia.org)
In the β-d-xylopyranoside treated cells, the biofilm level was only partially restored by the addition of cAMP, and these cells remained mainly as yeast-form cells. (lu.se)
A biofilm is an assemblage of surface-associated microbial cells that form an extracellular polymeric substance, named EPS-matrix. (maritech.org)
In addition, its effect on bacterial biofilms , the main life form of soil microbial communities , has not been adequately studied. (bvsalud.org)
Because of the potential to form biofilm, CDC recommends that all dental unit waterlines be treated regularly with disinfectants to meet the Environmental Protection Agency (EPA) regulatory standards for drinking water (i.e., ≤500 colony forming units (CFU)/mL of heterotrophic water bacteria). (cdc.gov)

For the study, first author Matthew Powers, an undergraduate student in Shank's lab, used a strain of B. subtilis--a species commonly used in lab experiments--that fluoresces when genes for biofilm formation are being shut off. (sciencedaily.com)
While whole genome sequencing has led to the identification of biofilm synthesis gene clusters in many bacterial species, bioinformatics has not identified the biofilm synthesis genes within the L. monocytogenes genome. (harvard.edu)
To identify genes necessary for L. monocytogenes biofilm formation, we performed a transposon mutagenesis library screen using a recently constructed Himar1 mariner transposon. (harvard.edu)
We used whole-transcriptome shotgun sequencing (RNA-seq) to compare the S. pneumoniae transcriptome in biofilms, bacteria dispersed from biofilms after exposure to IAV, febrile-range temperature, or ATP, and planktonic cells grown at 37°C. Compared with biofilm bacteria, actively dispersed S. pneumoniae, which were more virulent in invasive disease, upregulated genes involved in carbohydrate metabolism. (lu.se)
[10] [11] A cell that switches to the biofilm mode of growth undergoes a phenotypic shift in behavior in which large suites of genes are differentially regulated . (wikipedia.org)
Next to UFA biosynthesis, a number of these direct targets and other indirect targets identified by transcriptomics (e.g. ribosomal genes, ompA , ompC , ompX , osmB , osmC, sseI ), could possibly contribute to the effect of FabR on biofilm formation. (biomedcentral.com)
The differentially expressed genes and proteins linked to S. aureus biofilms have been identified using transcriptomic and proteomic investigations ( 14 - 17 ). (frontiersin.org)
Recent genomic and proteomic studies have identified many of the genes and gene products differentially expressed during biofilm formation, revealing the complexity of this developmental process. (biomedcentral.com)
With so many genes involved, it is perhaps not surprising that biofilm formation is regarded as a developmental process (Figure 1 ), not unlike that observed in the formation of fruiting bodies containing spores by the soil bacterium Myxococcus xanthus and sporulation in Bacillus subtilis [ 6 ]. (biomedcentral.com)

Additionally, it inhibits the physiological traits of the S. aureus biofilm, including hydrophobicity, motility, eDNA, and PIA associated to the biofilm. (frontiersin.org)
Stewart, P. S. & Franklin, M. J. Physiological heterogeneity in biofilms. (nature.com)

Pathogenic characteristics such as germ tube and biofilm formation and production of tissue damaging enzymes are possible targets of new drugs. (hindawi.com)
However, there have not been many investigations on how pathogenic bacteria are affected by the metabolic properties of planktonic and biofilm probiotics. (frontiersin.org)

Although anti-swarmers exhibit the potential to yield clinical benefits, it is important to be aware of possible implications regarding biofilm formation and antibiotic resistance. (cranberryinstitute.org)
S. aureus produces large biofilm formations that support its pathogenicity and confer protection and subsequently drug resistance ( 5 ). (frontiersin.org)
Compared with their planktonic (non-adherent) counterparts, the compact microbial consortia present in biofilms show extraordinary resistance to conventional biocides, antimicrobial treatments and the immune defense responses of the host. (biomedcentral.com)
[ 10 ] Multiple biofilm-specific mechanisms are operated simultaneously in a reversible and transient manner contributing to the high levels of antibiotic resistance of biofilms, and these are distinct from the well-characterized intrinsic resistance mechanisms (e.g., expression of antibiotic-degrading enzymes, inducible decrease in antibiotic influx, inducible increase in antibiotic efflux and alteration in antibiotic target sites) employed by planktonic cells. (medscape.com)
If the time required for an antibiotic to penetrate biofilms is longer than the duration of antibiotic treatment, the slower penetration will explain the antibiotic resistance. (medscape.com)

Approximately 10,000 transposon mutants within L. monocytogenes strain 10403S were screened for biofilm formation in 96-well polyvinyl chloride (PVC) microtiter plates with 70 Himar1 insertion mutants identified that produced significantly less biofilms. (harvard.edu)
Importantly, the most efficient inhibitors (Bri2 BRICHOS and DNAJB6) significantly reduce bacterial biofilm formation. (au.dk)
By day 15 of the study, the biofilms in the bandaged limb wounds had increased significantly in size and depth compared to those in uncovered leg wounds. (equusmagazine.com)
A comparison shows that the surface of CPVC is significantly more consistent and even than stainless steel, creating an environment less conducive for biofilm growth. (corzan.com)

A known antibiotic and antifungal compound produced by a soil microbe can inhibit another species of microbe from forming biofilms - -microbial mats that frequently are medically harmful -- without killing that microbe. (sciencedaily.com)
DAPG, or the DAPG-producing P. Protogens as a protobiotic, could be used to inhibit formation of harmful biofilms. (sciencedaily.com)
What was not known was whether biofilms inhibit the wound healing process or are a byproduct of the disruption. (equusmagazine.com)
Techniques to inhibit biofilm formation are essential and have wide applications. (biomedcentral.com)
The results clearly demonstrated that rhamnolipids inhibit biofilm formation after the initiation process, however, they do not affect attachment or maturation. (biomedcentral.com)
Rhamnolipids inhibit oral bacterial growth and biofilm formation by A. actinomycetemcomitans Y4, and may serve as novel oral drug against localized invasive periodontitis. (biomedcentral.com)

Biofilms are proven sources of contamination in the food industry. (foodsafetynews.com)
If food comes into contact with these biofilms, contamination may occur. (foodsafetynews.com)
We discovered further biofilms in drains and water hoses - places that are not cleaned by default, but are a potential source of contamination. (foodsafetynews.com)
What Are Biofilm Formation and Biological Contamination? (corzan.com)
Alfa and colleagues [ 2 ] looked at the impact of improper positioning of the elevator lever of a duodenoscope on bacterial persistence, biofilm formation, and endoscope contamination. (medscape.com)

Biofilm formation requires three different stages: cell attachment to a solid substrate, adhesion, and growth. (intechopen.com)
The berry polyphenolic preparation dose-dependently inhibited S. mutans biofilm formation while not reducing bacterial growth. (cranberryinstitute.org)
The negative implications of biofilm growth in the industry are enormous. (maritech.org)
Flow rates and turbulence can impact the concentration of nutrients and oxygen in the biofilm matrix, which can affect bacterial growth and EPS production. (scientiaen.com)
Rhamnolipids inhibited the growth and biofilm formation ability of all examined oral bacteria. (biomedcentral.com)
Thus, there is a requirement for the development of a simpler and more effective oral care capable of inhibiting oral bacterial growth and biofilm formation. (biomedcentral.com)

Biofilm is a complex matrix consisting of extracellular polysaccharides, DNA, and proteins that protect bacteria from a variety of physical, chemical, and biological stresses allowing them to survive in hostile environments. (intechopen.com)
Knowledge about the complex regulatory processes in biofilm formation can provide more insight into survival strategies of S. Typhimurium in non-host environments and can be the fundament of new eradication methods. (biomedcentral.com)
Biofilms play an important role in ecology and industry, as they are found in a variety of natural and man-made environments. (scientiaen.com)
Bacteria survive in various environments by forming biofilms. (biomedcentral.com)
Knowledge gained on presence and composition, published in the International Journal of Food Microbiology , could help to prevent and reduce biofilm formation within food processing environments. (foodsafetynews.com)

They can be found very early in Earth's fossil records (about 3.25 billion years ago) as both Archaea and Bacteria, and commonly protect prokaryotic cells by providing them with homeostasis, encouraging the development of complex interactions between the cells in the biofilm. (wikipedia.org)
Our work provides an extended understanding of the metabolic interplay in biofilms and presents a new approach of investigating complex interactions in bacterial communities on the systems level. (nature.com)
T. Bacillus subtilis biofilm formation and social interactions. (nature.com)
The process of biofilm formation is influenced by a variety of factors, including environmental conditions, bacterial genetics, and microbial interactions. (scientiaen.com)

All isolates were found capable of biofilm formation in a microtitre plate assay. (banglajol.info)
The observations were largely in concordance with the biofilm assay results. (biomedcentral.com)

Major shifts in biofilm composition, with a decrease in prevalence of both cariogenic species and periodontopathogens, were induced after powered brushing using an antibacterial-regimen. (rug.nl)
Some cave biofilms have very few species, 10 to 20. (karstworlds.com)
Biofilms are made up of thin layers of microbe species that can be very different. (karstworlds.com)
Because the cave biofilms are relatively simple, it will be easier to connect the various microbe species to the geochemistry involved. (karstworlds.com)
Biofilms can be composed of a population that developed from a single species or a community derived from multiple microbial species ( 14 , 17 ). (cdc.gov)

Biofilms were grown on coverslips in the presence of plant extracts and processed for scanning electron microscopy (SEM). (hindawi.com)
To corroborate these results, biofilms were observed by scanning electron microscopy (SEM) and confocal microscopy. (biomedcentral.com)

abstract = "Objectives: Orthodontic, multi-strand retention-wires are used as a generalized model for oral retention sites to investigate whether biofilm left-behind after powered toothbrushing in-vivo enabled better penetration of antibacterials as compared with manual brushing.Methods: 2-cm multi-strand, stainless-steel retention-wires were placed in brackets bonded bilaterally in the upper arches of 10-volunteers. (rug.nl)

Enzymatic assays for ATP and lactate production confirmed that dispersed pneumococci exhibited increased metabolism compared to those in biofilms. (lu.se)
Here, we used untargeted metabolomics to investigate the potential impacts of planktonic bacteria (LAB-pk-CFS) and biofilm colonies (LAB-bf-CFS) on the metabolism of S. aureus biofilms. (frontiersin.org)

Anti-bacterial agents and disinfectants are effective against planktonic bacteria (bacteria suspended in liquid), however, they are not effective against biofilm-forming bacteria due to the difficulty of chemicals penetrating the biofilm. (biomedcentral.com)

Also, vinegar rinsing could reduce bacterial viability and disrupt the 24-h biofilm. (biomedcentral.com)
Powered-toothbrushing combined with antibacterial-regimens yielded lower biofilm viability than manual brushing, indicating better antibacterial penetration into biofilm left-behind after powered brushing. (rug.nl)
Biofilm formation and bacterial viability during the survival analysis were assessed. (medscape.com)

With time we observed formation of putative calcium carbonate crystals, located along the edges and within the colony ( Figure 1b ). (nature.com)
Understanding how cave biofilms dissolve calcium carbonate may help us to understand the communities around ocean floor vents, but it may also, eventually, lead to understanding how biofilms dissolve calcium phosphate on teeth and the steel hulls of ships. (karstworlds.com)

At the next stage (3), the first maturation phase is reached, as indicated by early development of biofilm architecture. (biomedcentral.com)

The biofilm matrix consists of polysaccharides, proteins, and DNA and constitutes a stubborn source that protects bacteria from a variety of physical, chemical, and biological stresses. (intechopen.com)
[2] [3] The cells within the biofilm produce the EPS components, which are typically a polymeric conglomeration of extracellular polysaccharides , proteins , lipids and DNA . (wikipedia.org)

Our study identified a novel mechanism maintaining Bacillus subtilis and Mycobacterium smegmatis biofilms-active production of calcite minerals. (nature.com)

But a comparison of the differentially expressed gene sets identified in several recent DNA microarray studies [ 7 - 10 ] reveals that no common expression pattern for biofilms has yet emerged. (biomedcentral.com)

These components help to create a complex three-dimensional structure that provides the biofilm community with protection from environmental stresses and access to nutrients. (scientiaen.com)
[ 20 ] Starvation is also found in biofilms owing to nutrient consumption by peripheral cells and reduced diffusion of oxygen and nutrients through biofilms. (medscape.com)

Although physical methods are fundamental for removing oral biofilms, there is a wide variation in its efficiency due to individual differences. (biomedcentral.com)
These methods can be expensive and, in the instance of hot steam cleaning, leave residual moisture for biofilm to harbor. (corzan.com)

Legionnaires' disease is a potentially fatal pneumonia caused by Legionella pneumophila , an aquatic bacterium often found within the biofilm niche. (frontiersin.org)

Biofilm quantification was done by microtiter method. (magiran.com)

We studied, for the first time, the effects of mutants defective in biomineralization and calcite formation on biofilm development, resilience and morphology. (nature.com)

This study focused on the changes in biofilm activity and related metabolic pathways of S. aureus treated with lactic acid bacteria planktonic CFS (LAB-pk-CFS) and biofilm state (LAB-bf-CFS). (frontiersin.org)
Fig. 6: Local cycling of nucleotide and metabolic division of labor at biofilm periphery. (nature.com)
Speculations about the ecologic advantages of forming a biofilm include protection from the environment, nutrient availability, metabolic cooperation, and acquisition of new genetic traits ( 3 , 17 ). (cdc.gov)
All the studied concentrations of the pesticide completely or partially suppress the matrix and structure of the E. coli CDC F-50 biofilm formation, as well as the bacterial cells metabolic activity in the biofilm . (bvsalud.org)

Anatomy1

Organisms13

Diseases2

Chemicals and Drugs26

Analytical, Diagnostic and Therapeutic Techniques and Equipment9

Phenomena and Processes14

Disciplines and Occupations1

Technology, Industry, Agriculture6

Information Science1

Health Care4

Microorganisms18

Ability to form biofilms4

Pseudomonas5

Strains12

Surfaces15

Microbes9

Prevent biofilm formation1

Extracellular matrix3

Aeruginosa8

Stages of biofilm formation1

Multi-species biofilms2

Affect biofilm formation1

Staphylococcus2

Anti-biofilm agents2

Adherence4

Disinfection5

Swarming motility1

Candida5

Vitro1

Pathogens2

Escherichia3

Control of biofilms1

Bacteria within biofilms1

Infections12

Mature biofilms1

Streptococcus2

Antibiotics10

Oral biofilm6

Strong biofilm2

Salmonella5

Hyphae formation2

Form10

Genes9

Physiological2

Pathogenic2

Resistance5

Significantly4

Inhibit6

Contamination5

Growth6

Environments5

Interactions4

Assay2

Species5

Scanning electron m2

Abstract1

Metabolism2

Planktonic bacteria1

Viability3

Calcium carbonate2

Maturation1

Polysaccharides2

Bacillus1

Differentially expressed1

Nutrients2

Methods2

Bacterium1

Quantification1

Mutants1

Metabolic4