AnatomyOrganismsDiseasesChemicals and DrugsAnalytical, Diagnostic and Therapeutic Techniques and EquipmentPhenomena and ProcessesInformation ScienceGeographicals
Lassa virusLassa FeverArenaviridaeGlycoproteinsGuineaViral Envelope ProteinsSierra LeoneArenavirusbeta 2-Glycoprotein IAfrica, WesternArenaviruses, Old WorldArenaviridae InfectionsMolecular Sequence DataMurinaeHemorrhagic Fevers, ViralPlatelet Glycoprotein GPIb-IX ComplexGlycopeptidesAmino Acid SequencePichinde virusOrosomucoidMyelin-Associated GlycoproteinLiberiaMembrane GlycoproteinsGlycosylationPatient IsolatorsPlatelet Membrane GlycoproteinsCell LinePlatelet Glycoprotein GPIIb-IIIa ComplexAntibodies, ViralViral ProteinsVero CellsCercopithecus aethiopsArenaviruses, New WorldAntibodies, MonoclonalViral VaccinesMolecular WeightGlycoprotein Hormones, alpha SubunitBase SequenceMarburgvirusAntigens, ViralVirus InternalizationCarbohydratesCricetinaeOligosaccharidesEpitopesEbolavirusRNA VirusesMyelin-Oligodendrocyte GlycoproteinBlood PlateletsElectrophoresis, Polyacrylamide GelReceptors, VirusLymphocytic choriomeningitis virusNucleoproteinsCarbohydrate SequenceMarburg Virus DiseaseHIV Envelope Protein gp120PolysaccharidesLectinsRecombinant ProteinsNeutralization TestsDystroglycansProtein Processing, Post-TranslationalVirion
Caused by Lassa virusVaccineLASVVirusesEbola Virus GlycoproteinWest AfricaNucleoproteinArenavirusAntibodiesViral envelopeStrainInfectionsMarburgEnvelopeJuninHemorrhagic feverMastomysAntigenFever is a severeInfectsInfectiousInfectionMatrix proteinLineagesParticlesSubunitsSurvivorsAntibodyIllnessStableReservoirSevereSurface
Caused by Lassa virus4
Vaccine28
  • Like other arenaviruses, Lassa virus lacks a ogy and clinical presentation, treatment, prevention conventional negative-strand coding arrangement and control as well as the current theories of its patho- and the isolates of the virus differ in their genetic, genesis and efforts in vaccine development. (folkhalsomyndigheten.se)
  • With thousands dying from Lassa every year-and the potential for the virus to cause even larger outbreaks-researchers are committed to designing a vaccine to stop it. (gvn.org)
  • Importantly, the new structure provides a guide for designing a Lassa virus vaccine. (gvn.org)
  • She then used this model glycoprotein as a sort of magnet to find antibodies in patient samples that could bind with the glycoprotein to neutralize the virus.The molecular structure of the Lassa virus glycoprotein trimer provides the blueprints for vaccine design. (gvn.org)
  • The molecular structure of the Lassa virus glycoprotein trimer provides the blueprints for vaccine design. (gvn.org)
  • ChAdOx1-vectored Lassa fever vaccine elicits a robust cellular and humoral immune response and protects guinea pigs against lethal Lassa virus challenge. (ox.ac.uk)
  • ChAdOx1-Lassa-GPC is a chimpanzee adenovirus-vectored vaccine encoding the Josiah strain LASV glycoprotein precursor (GPC) gene. (ox.ac.uk)
  • Lassa virus has a single antigen on its surface, a viral glycoprotein that is the focus of vaccine efforts. (jenner.ac.uk)
  • Vaccine platforms for the prevention of Lassa fever. (jenner.ac.uk)
  • Oxidation-sensitive polymersomes as vaccine nanocarriers enhance humoral responses against Lassa virus envelope glycoprotein.Oxidation-sensitive polymersomes as vaccine nanocarriers enhance humoral responses against Lassa virus envelope glycoprotein. (tankfishtips.com)
  • Helix Biogen Institute (Known as Helix Biogen Consult) in partnership with Thelvax has initiated the development of COVID-19 candidate vaccine containing antigenic component of surface glycoprotein of SARS-CoV-2 which was expressed in E. coli. (helixbiogeninstitute.org)
  • Helix biogen Institute in collaboration with Thelvax commence the development of COVID-19 candidate 2 vaccine which also contain antigenic component of surface glycoprotein of SARS-CoV-2 expressed in insect. (helixbiogeninstitute.org)
  • Lassa virus infections can respond to antiviral treatment in some cases, but there is no vaccine available. (helixbiogeninstitute.org)
  • Helix Biogen Institute is working on a Lassa virus vaccine containing both nucleoprotein and surface glycoprotein component of the Lassa virus for endemic region. (helixbiogeninstitute.org)
  • The Institute is ideally placed to develop this new conjugate sub-unit vaccine to help prevent and ultimately eliminate Lassa virus in African countries where they are most needed. (helixbiogeninstitute.org)
  • Helix Biogen Institute is working on the development of a Lassa virus vaccine (candidate 2) which contain the surface glycoprotein component of the Lassa virus. (helixbiogeninstitute.org)
  • Lassa virus vaccine (candidate 3) that contains only nucleotide proteins is under development at Helix Biogen Institute. (helixbiogeninstitute.org)
  • INOVIO INO-4500 is a DNA vaccine candidate to prevent Lassa Fever. (precisionvaccinations.com)
  • INO-4700 is a DNA plasmid vaccine candidate that expresses the MERS CoV spike (S) glycoprotein. (precisionvaccinations.com)
  • Elucidating the adaptive immune characteristics of natural protection to Lassa fever (LF) is vital in designing and selecting optimal vaccine candidates. (kent.ac.uk)
  • With rejuvenated interest in LF and a call for accelerated research on the Lassa virus (LASV) vaccine, there is a need to define the correlates of natural protective immune responses to LF. (kent.ac.uk)
  • Lastly, as potential vaccine targets, we identified the regions of the LASV Glycoprotein (GP) and Nucleoprotein (NP) that induced the broadest peptide-specific T cell responses. (kent.ac.uk)
  • The journal is published per article and covers all areas of the subject such as: Isolation of chikungunya virus from non-human primates, Functional analysis of Lassa virus glycoprotein from a newly identified Lassa virus strain for possible use as vaccine using computational methods, as well as Molecular approaches towards analyzing the viruses infecting maize. (academicjournals.org)
  • Additionally, GeoVax's vaccine against Lassa Fever virus (LASV) is progressing in preclinical studies with funding support from the U.S. Department of Defense. (gabio.org)
  • When VLPs for viruses like COVID-19, Ebola, Marburg, Lassa fever or HIV are produced in vivo , they include not only the protein antigens, but also consist of membranes from the vaccinated individual's cells displaying vaccine proteins. (geovax.com)
  • These strategies may also be applicable towards the development of an urgently needed live-attenuated Lassa virus vaccine. (hhs.gov)
  • ZMapp), anti-sense phosphorodiamidate morpholino oligomers (PMO AVI-6002), lipid nanoparticle small interfering RNA (LNP-siRNA: TKM-Ebola), and an EBOV glycoprotein-based vaccine using live-attenuated recombinant vesicular stomatitis virus (rVSV-EBOGP) or a chimpanzee adenovirus (rChAd-EBOGP)-based vector. (biomedcentral.com)
  • TWiV reviews a Lassa virus mRNA vaccine that confers protection against disease without inducing neutralizing antibodies, and a CRISPR-based method for engineering the genome of RNA viruses. (microbe.tv)
LASV8
  • The epitopes of the Lassa virus (LASV) surface glycoproteins recognized by naturally infected human hosts have not been identified or characterized. (bvsalud.org)
  • Here we have cloned 113 human monoclonal antibodies (mAbs) specific for LASV glycoproteins from memory B cells of Lassa fever survivors from West Africa . (bvsalud.org)
  • Notably, of the 16 mAbs that neutralize LASV, 13 require the assembled glycoprotein complex for binding, while the remaining 3 require GP1 only. (bvsalud.org)
  • Lassa virus (LASV) infects hundreds of thousands of individuals each year, highlighting the need for the accelerated development of preventive, diagnostic, and therapeutic interventions. (ox.ac.uk)
  • Furthermore, a single dose of ChAdOx1-Lassa-GPC fully protects Hartley guinea pigs against morbidity and mortality following lethal challenge with a guinea pig-adapted LASV (strain Josiah). (ox.ac.uk)
  • A prime-boost regimen of ChAdOx1-Lassa-GPC in guinea pigs significantly increased antigen-specific antibody titers and cleared viable LASV from the tissues. (ox.ac.uk)
  • Lassa computer virus (LASV), a member of the family species complex were identified as the Piperonyl butoxide natural host of LASV in certain countries in West Africa, including Sierra Leone, Nigeria, Guinea, and Liberia (26, 35, 49). (esbiomech2012.org)
  • Furthermore, we have elucidated how Lassa computer virus proteins interact to direct budding and release of LASV progeny from epithelial cell layers. (esbiomech2012.org)
Viruses8
  • The Lassa virus is a member of the Arenaviridae family of viruses. (wikipedia.org)
  • This article divides the discussion of these viruses by clinical relevance and manifestations: Old World (Lassa virus, LCMV) and New World (South American hemorrhagic fevers). (medscape.com)
  • Dr. Garry is the President of the Viral Hemorrhagic Fever Consortium (VHFC), a non-profit consortium of scientists developing countermeasures including diagnostics, immunotherapeutics and vaccines, against Lassa virus, Ebola and Marburg viruses and other high consequence pathogens. (zalgen.com)
  • It was great to see exactly how Lassa was different from other viruses," said Hastie, 10 years after starting the project. (gvn.org)
  • Lassa virus uses an endocrytic pathway that lacks clathrin, caveolin, dynamin, and actin unlike other enveloped viruses. (bestnursingpapers.com)
  • With a primary focus on West Nile, Zika, Ebola, and Lassa viruses, we investigate why this is the case by providing system-wide understandings of the interaction and co-evolution between host and pathogen. (andersen-lab.com)
  • By manipulating the genome of vesicular stomatitis virus we generate recombinant autonomously propogating viruses in which the single VSV attachment and fusion glycoprotein (G) has been replaced by the entry machinery of representatives from every family of enveloped viruses. (whelan-lab.org)
  • The new consortium will focus on clinical development of therapeutic candidates for two classes of deadly viruses represented by Ebola and Lassa, as well as alphaviruses such as chikungunya and equine encephalitis viruses that sicken millions of people around the world. (vumc.org)
Ebola Virus Glycoprotein1
  • ATLANTA, GA, October 14, 2021 - GeoVax Labs, Inc . (Nasdaq: GOVX), a biotechnology company developing human immunotherapies and vaccines against infectious diseases and cancer, today announced that the U.S. Patent and Trademark Office has issued a Notice of Allowance for Patent Application No. 15/543,139 entitled "Replication-Deficient Modified Vaccinia Ankara (MVA) Expressing Ebola Virus Glycoprotein (GP) and Matrix Protein (VP40) . (gabio.org)
West Africa9
Nucleoprotein2
  • EBOV nucleoprotein (NP), soluble glycoprotein, or glycopro- approaches, which successfully protected rodents from lethal infection, were protective for cynomolgus or rhesus macaques challenged with EBOV. (cdc.gov)
  • A single inoculation of a plasmid encoding full-length Lassa nucleoprotein (LNP) can induce CD8 (+) T cell responses in mice and protect them against infection with two arenaviruses, LCMV and Pichinde virus (PV). (helixbiogeninstitute.org)
Arenavirus2
  • Their study, published June 2, 2017 in the journal Science , is the first to show a key piece of the viral structure, called the surface glycoprotein, for any member of the deadly arenavirus family. (gvn.org)
  • Lassa virus, the causality of the Lassa fever belongs to genus arenavirus and arenavidae family (Clercq & Kern, 2003). (bestnursingpapers.com)
Antibodies7
  • Most neutralizing human monoclonal antibodies target novel epitopes requiring both Lassa virus glycoprotein subunits. (bvsalud.org)
  • Antibodies with broad neutralizing activity are induced by adjuvant-formulated virus-like particles expressing native-like forms of the Lassa virus envelope surface glycoprotein. (helixbiogeninstitute.org)
  • The therapeutic role for non-neutralizing antibodies in preventing disease is becoming more evident for high-risk pathogens such as Ebola, Lassa, and Nipah virus. (sciencedaily.com)
  • Monoclonal antibodies to murine hepatitis virus-4 (strain JHM) define the viral glycoprotein responsible for attachment and cell-cell fusion. (microbiologyresearch.org)
  • Naturally Acquired Rift Valley Fever Virus Neutralizing Antibodies Predominantly Target the Gn Glycoprotein. (ox.ac.uk)
  • Numerous animal studies have demonstrated that protective immunity is acquired following RVF virus (RVFV) infection and that this correlates with acquisition of virus-neutralizing antibodies (nAbs) that target the viral envelope glycoproteins. (ox.ac.uk)
  • For several years Saphire, who will lead the Ebola project, has worked with Crowe and his team to improve the ability of antibodies to identify and destroy Ebola and a related virus, Marburg, by recognizing specific glycoproteins on the surfaces of the viral particles. (vumc.org)
Viral envelope3
  • The viral envelope or covering is studded with glycoproteins comprising of tetrameric composites that belong to viral glycoproteins GP1 and GP2. (bestnursingpapers.com)
  • Here, we characterized the immune response to the viral envelope glycoproteins, Gn and Gc, in RVFV-exposed Kenyan adults. (ox.ac.uk)
  • While below the resolution of these micrographs, both types of VLPs display the native form of their respective viral envelope glycoproteins which we believe is key to generating an effective immune humoral response. (geovax.com)
Strain2
  • This virus has both a large and a small genome section, with four lineages identified to date: Josiah (Sierra Leone), GA391 (Nigeria), LP (Nigeria) and strain AV. Lassa virus commonly spreads to humans from other animals, specifically the Natal multimammate mouse or African rat, also called the Natal multimammate rat (Mastomys natalensis). (wikipedia.org)
  • The live- attenuated Candid#1 strain of Junín virus (JUNV) is currently used in Argentina to protect against Argentine hemorrhagic fever, but this virus carries the distinct liability that attenuation is solely dependent on a single phenylalanine-to-isoleucine substitution at position 427 (F427I) in the GP2 fusion subunit of the JUNV envelope glycoprotein (GPC). (hhs.gov)
Infections5
  • About 1% of all Lassa virus infections result in death. (wikipedia.org)
  • Most infections due to the Lassa virus are mild or subclinical. (medscape.com)
  • We employ mNGS, experimentation and evolutionary theory to conduct research on West Nile virus, Zika virus, Ebola virus, and Lassa virus infections. (andersen-lab.com)
  • Children and women in late pregnancy are particularly vulnerable to Lassa virus infections, for which there are no vaccines or approved treatments. (vumc.org)
  • Moreover, in addition to our understanding of the glycosylation of dystroglycan, much insight has also been gained into the functional roles of dystroglycan, not only in the bran and muscle, but also in other cells types, and the implication that the defective processing of this protein has with respect to role in Lassa fever virus infections. (enmc.org)
Marburg1
  • computer simulations of proteolysis of marburg and ebola-zaire filovirus coded proteins to generate nonapeptides with motifs of known hla class i haplotypes and detection of antigenic domains in the viral glycoproteins. (liverpool.ac.uk)
Envelope2
  • Mechanistically, neuraminidase inhibition assay and hemagglutination inhibition assay suggested that N30 did not directly target the two envelope glycoproteins required for viral adsorption or release. (biomedcentral.com)
  • In addition, we examined the inhibitory rate of the compound against two IAV envelope glycoproteins, hemagglutinin and neuraminidase, and investigated its effect on expression and the enzyme activity of IMPDH type II. (biomedcentral.com)
Junin1
  • Reverse genetics generation of chimeric infectious Junin/Lassa virus is dependent on interaction of homologous glycoprotein stable signal peptide and G2 cytoplasmic domains. (cdc.gov)
Hemorrhagic fever3
  • Lassa fever, also known as Lassa hemorrhagic fever, is a type of viral hemorrhagic fever caused by the Lassa virus. (wikipedia.org)
  • Crimean-Congo hemorrhagic fever virus glycoprotein proteolytic processing by subtilase SKI-1. (wikigenes.org)
  • These include Lassa virus, Rift Valley fever virus, Crimean-Congo hemorrhagic fever virus and many hantaviruses. (ox.ac.uk)
Mastomys1
Antigen1
  • By utilizing the favorable immune cell targeting of the Ebola glycoprotein, better foreign antigen presentation can be achieved. (nih.gov)
Fever is a severe1
  • Lassa fever is a severe multisystem disease that often has haemorrhagic manifestations. (bvsalud.org)
Infects1
  • Although less well-known than Ebola, Lassa infects thousands of people in Africa annually. (vumc.org)
Infectious1
  • This paper discusses Lassa fever, an infectious disease with regard to its causality organism, transmission, epidemiology, and factors causing the illness, clinical manifestation and diagnosis and the role of the nurse in controlling the illness. (bestnursingpapers.com)
Infection1
  • These data support further development of ChAdOx1-Lassa-GPC and testing in non-human primate models of infection. (ox.ac.uk)
Matrix protein2
  • Spiropoulou, J. finding that, when expressed individually, both the glycoprotein GP and matrix protein Z form virus-like particles. (esbiomech2012.org)
  • We show that GP determines the apical release of Lassa computer virus from epithelial cells, presumably by recruiting the matrix protein Z to the site of computer virus assembly, which is usually in turn essential for nucleocapsid incorporation into virions. (esbiomech2012.org)
Lineages2
Particles1
  • For example, we are combining tomographic subvolume averaging techniques and high-resolution single particle electron cryomicroscopy with fitting of X-ray structures of viral glycoproteins into density maps of whole virus particles. (ox.ac.uk)
Subunits3
  • One-half bind the GP2 fusion subunit, one-fourth recognize the GP1 receptor-binding subunit and the remaining fourth are specific for the assembled glycoprotein complex, requiring both GP1 and GP2 subunits for recognition. (bvsalud.org)
  • The problem was that glycoproteins are made up of smaller subunits. (gvn.org)
  • Another challenge was to recreate part of the viral lifecycle in the lab-a stage when Lassa's glycoprotein gets clipped into two subunits. (gvn.org)
Survivors1
  • Lassa virus (LV), for example, is a common cause of disease in Africa, and survivors frequently suffer from significant neurological damage. (helixbiogeninstitute.org)
Antibody2
  • At last, she solved the structure of the Lassa virus glycoprotein, bound to a neutralizing antibody from a human survivor. (gvn.org)
  • In the following study, we show that ChAdOx1-Lassa-GPC is immunogenic, inducing robust T-cell and antibody responses in mice. (ox.ac.uk)
Illness1
  • Lassa fever is an acute viral haemorrhagic illness. (jenner.ac.uk)
Stable1
  • The technique depends on having a stable protein, yet all the Lassa virus glycoprotein wanted to do was fall apart. (gvn.org)
Reservoir1
Severe1
  • The overall case fatality of Lassa fever is around 1-2% often due to multiorgan failure, but severe disease is found in around 20% of cases with difficulty breathing and blood loss (haemorrhaging) common symptoms. (jenner.ac.uk)
Surface2
  • To overcome these obstacles, Hastie created mutant versions of important parts of the molecule and engineered a version of the Lassa virus surface glycoprotein that didn't fall apart. (gvn.org)
  • Ergonul & Whitehouse (2007) affirms that Lassa virus has receptors on its cell surface called the alpha-dystroglycan (alpha-DG) used for gaining access into the host cell. (bestnursingpapers.com)