Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) was employed to characterize the polygalloyl polyflavan-3-ols (PGPF) in grape seed extracts. (nih.gov)
In recent years matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has emerged as a tool for microbial identification and diagnosis. (matis.is)
Evaluation of the Andromas matrix-assisted laser desorption ionization-time of flight mass spectrometry system for identification of aerobically growing Gram-positive bacilli. (pasteur.fr)
Matrix-associated laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) is a rapid and simple microbial identification method. (pasteur.fr)
Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS) is widely used in clinical laboratories for routine identification of bacteria and yeasts. (knaw.nl)
Dive into the research topics of 'A New Filter Based Cultivation Approach for Improving Aspergillus Identification using Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS)'. Together they form a unique fingerprint. (knaw.nl)
A New Filter Based Cultivation Approach for Improving Aspergillus Identification using Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS) . Mycopathologia , 187 (1), 39-52. (knaw.nl)
Dive into the research topics of 'Efficient identification of Malassezia yeasts by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS)'. Together they form a unique fingerprint. (uab.cat)
Efficient identification of Malassezia yeasts by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) . British Journal of Dermatology , 170 , 332-341. (uab.cat)
Ma-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has been reported to be a rapid and reliable diagnostic tool to identify clinically important yeasts, but so far no data have been reported on identification of Malassezia isolates with this technique. (uab.cat)
Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has been developed for the rapid identification of microorganisms and proved to be a robust tool for the recognition of bacterial species. (uab.cat)
Although the matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) systems are relatively less studied, they have been reported to be a promising method for the identification of anaerobes. (elsevierpure.com)
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) was used for species determination. (lu.se)
Cell proteins were analysed with 2-dimensional electrophoresis and differentially expressed proteins were identified by matrix-assisted laser desorption/ ionization time-of-flight mass spectrometry. (who.int)
Two-dimensional differential gel electrophoresis (2D-DIGE) and matrix-assisted laser desorption/ionization-time of flight-mass spectrometry (MALDI-TOF-MS) based proteomics was used to study differential protein abundance in various B cell types during B cell maturation (cell lines are listed in Supplementary Table 1 ). (lu.se)
Surface-assisted laser desorption2
Surface-assisted laser desorption/ionization mass spectrometry (SALDI-MS) is ideally suited for the high-throughput analysis of small molecules in bodily fluids (e.g. saliva, urine, and blood plasma). (bsz-bw.de)
In this study, we propose a simple and rapid technique for characterization of free fatty acids and triacylglycerides (TAG) based on palladium nanoparticular (Pd-NP) surface-assisted laser desorption/ionization (SALDI) mass spectrometry (MS). The implemented Pd-NP material allowed detection of free fatty acids and TAGs exclusively as [M + K]+ ions in positive ion mode. (tib.eu)
MALDI10
Matrix-assisted laser desorption electrospray ionization (MALDESI) was first introduced in 2006 as a novel ambient ionization technique which combines the benefits of electrospray ionization (ESI) and matrix-assisted laser desorption/ionization (MALDI). (wikipedia.org)
The original MALDESI design was implemented using common organic matrices, similar to those used in MALDI, along with a UV laser. (wikipedia.org)
This thesis encompasses work done to aid in the incorporation of matrix-assisted laser desorption/ionization - mass spectrometry imaging (MALDI-MSI) into the forensic workflow. (ojp.gov)
Here we report the first application of a matrix-assisted laser desorption/ionization (MALDI) triplequadrupole mass spectrometer for targeted proteomics. (canada.ca)
The technique was then applied to validate an important virulence biomarker of the fungal pathogen Candida albicans, which was not accurately quantified using global proteomics experiment employing two-dimensional liquid chromatography/electrospray ionization tandem mass spectrometry (2D-LC/ESI)-MS/MS. Using LC/MALDI-MRM analysis of five tryptic peptides, the protein PHR1 was found to be upregulated in the hyphal (pathogenic) form of C. albicans by a factor of 7.7W0.8. (canada.ca)
Matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry, and its associated analytic software, was recently approved for clinical microbiology diagnostic use by the United States Food and Drug Administration. (temple.edu)
A method based on the analyte-to-matrix ion abundance ratio in the matrix-assisted laser desorption ionization (MALDI) spectrum was used for the quantification of phosphatidylcholine (PC) in mouse brain tissue. (kribb.re.kr)
Herein, we report that breast cancer (BC) patients can be distinguished from cancer-free (NC) controls by serum immunoglobulin G (IgG) crystallizable fragment (Fc) region N-glycosylation profiling using matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS). Recently, there has been much progress in the field of tumor immunology. (elsevierpure.com)
Metabolite characterization was conducted with matrix assisted laser desorption ionization (MALDI) and liquid chromatography mass spectrometry (LC-MS). The metabolomics analysis revealed a significant increase in 44 metabolites in BALF, including 1,2-Dipalmitoyl-sn-glycero-3-phosphoglycerol (DPPG) and cholesterol in the rats exposed to 112 microg at the 7- and 28-day time points. (cdc.gov)
We used 3 different approaches: biochemical testing, matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry, and 16S ribosomal RNA (rRNA) gene sequencing. (medscape.com)
Ions1
The matrix absorbs the laser energy, thus inducing a soft disintegration of the sample-matrix mixture into free (gas phase) matrix and analyte molecules and molecular ions. (rush.edu)
Mass10
The experimental settings involved in the communication between the sampling stage, mass spectrometer and laser desorption, such as stage height, ESI-Spot distance and laser repetition rate, have undergone progressive optimization by several design of experiments (DOE). (wikipedia.org)
Matrix-Assisted Laser Desorption Ionization-Mass Spectrometry Signal E" by N Rodthongkum, YB Chen et al. (umass.edu)
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (rush.edu)
This graph shows the total number of publications written about "Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization" by people in this website by year, and whether "Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization" was a major or minor topic of these publications. (rush.edu)
Below are the most recent publications written about "Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization" by people in Profiles. (rush.edu)
Modern flight-capable mass spectrometers, in combination with various sample processing, separation, and ionization techniques enable sensitive detection of chemical biosignatures. (frontiersin.org)
To this end database search engines were rapidly developed after the introduction of ionization techniques for biological mass spectrometry [1-5]. (lu.se)
Analysis of carbohydrates and glycoconjugates by matrix-assisted laser desorption/ionization mass spectrometry: An update for 2017-2018. (bvsalud.org)
Absorbs1
The term 'matrix' refers to any molecule that is present in large excess and absorbs the energy of the laser, thus facilitating desorption of analyte molecules. (wikipedia.org)
Molecules1
Analyte molecules are embedded in an excess matrix of small organic molecules that show a high resonant absorption at the laser wavelength used. (rush.edu)
Ultraviolet2
An infrared (IR) or ultraviolet (UV) laser can be utilized in MALDESI to resonantly excite an endogenous or exogenous matrix. (wikipedia.org)
Each sample is irradiated by 240 brief pulses from an ultraviolet nitrogen laser (337 nm), desorbed from the plate into the gas phase and ionized. (matis.is)
Negative ionization2
In a study to improve the detection of tissue-specific lipids, the electrospray parameters have been tailored for positive and negative ionization polarities. (wikipedia.org)
Under negative ionization conditions, unusual trimetric structures were generated for free fatty acids, while TAGs underwent irreproducible degradation reactions. (tib.eu)
Detection1
The ESI-like ionization mechanism has been experimentally demonstrated and studied in depth, showing equivalent softness as ESI and enabling the detection of intact protein complexes. (wikipedia.org)
Matrix2
The current MALDESI source employs endogenous water or a thin layer of exogenously deposited ice as the energy-absorbing matrix where O-H symmetric and asymmetric stretching bonds are resonantly excited by a mid-IR laser. (wikipedia.org)
Nursing Central , nursing.unboundmedicine.com/nursingcentral/view/Tabers-Dictionary/737987/all/matrix_assisted_laser_desorption_ionization. (unboundmedicine.com)
Source1
A water-cooled Peltier thermoelectric plate is used to control the sample stage temperature (−10 °C to 80 °C). The source has single- or multi-shot capabilities with adjustable laser fluence, repetition rate, and delay between the laser trigger and MS ion accumulation. (wikipedia.org)
Mode1
A mid-IR laser that excites the O-H stretching mode of water is used to desorb the neutral materials from the biological samples. (wikipedia.org)
Time of Flight Ma19
To decrease the rate misidentifications, an accurate identification method, such as matrix-assisted laser desorption ionization time of flight mass spectrometry or molecular methods, should be used. (hindawi.com)
S. lugdunensis , identified by superior methods, such as the recently described matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry-(MALDI TOF MS-) based identification [ 7 ], could be used to redefine the species of S. lugdunensis and newly investigate their prevalence and pathogenicity. (hindawi.com)
We aimed to establish a matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) spectrum database based on the analysis of the legs of six tick vectors: Amblyomma variegatum, Rhipicephalus sanguineus, Hyalomma marginatum rufipes, Ixodes ricinus, Dermacentor marginatus, and Dermacentor reticulatus. (nih.gov)
Matrix-assisted laser desorption ionization time-of-flight mass spectrometry, which has revolutionized the routine identification of microorganisms in clinical microbiology laboratories, was recently successfully applied to the identification of arthropod vectors. (hal.science)
In this study, we summarize the state-of-the-art of matrix-assisted laser desorption ionization time-of-flight mass spectrometry applied to the identification of arthropod vectors (ticks, mosquitoes, phlebotomine sand-flies, fleas, triatomines, lice and Culicoides), their trophic preferences and their ability to discriminate between infection statuses. (hal.science)
본 연구에서는 matrix-assisted laser desorption ionization-time of flight mass spectrometry(MALDI-TOF MS)를 이용한 carbapenemase 검출법에 대한 평가를 시행하였고 기존의 carbapenemase 검출법들과 비교 평가하였다. (dsmc.or.kr)
In this study, the performance of matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) was evaluated for the detection of carbapenemase and compared with the results of a modified Hodge test (MHT), a three-dimensional extract (3-D) bioassay, imipenem hydrolysis, and a molecular test. (dsmc.or.kr)
The relative binding strength of a series of terpyridine metal complexes of the type [M(II)L(2)](+) was investigated by using variable laser intensities in matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS). (tue.nl)
To evaluate the application of weak cation exchange (WCX) magnetic bead-based Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS) in detecting differentially expressed proteins in the urine of renal clear cell carcinoma (RCCC) and its value in the early diagnosis of RCCC. (cams.cn)
Protein fingerprinting using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) provides a rapid, reliable and economical alternative method of characterisation of fungal isolates. (supsi.ch)
In order to establish rapid and accurate typing method for Bacillus coagulans strains which is important for controlling in some canned foods and tea-based beverages manufacturing because of the high-heat resistance of the spores and high tolerance of the vegetative cells to catechins and chemicals, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and repetitive-PCR (rep-PCR) were evaluated. (elsevierpure.com)
Matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS) is a promising and fast method for identifying fungi and bacteria directly from positive blood cultures. (ku.dk)
Microflex LT (Bruker Daltonics) and VITEK MS (bioMérieux) are bacterial identification systems that are based on matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). For VITEK MS, two identification softwares, VITEK MS IVD (IVD) and SARAMIS (SARAMIS), are available. (elsevierpure.com)
Differentially expressed separated proteins were subsequently identified by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF/TOF-MS). Then, transthyretin (TTR), one of the differentially expressed proteins, was validated through real-time PCR, western blot and immunohistochemistry. (nih.gov)
Identification of bacterial species was performed with matrix-assisted laser desorption/ionization-time of flight mass spectrometry and pneumococci were serotyped using multiplex polymerase chain reactions. (springer.com)
In this work we identified these protein subunits with the modern proteomic tool of one-dimensional SDS-PAGE and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS). The three separated pMMO subunits of 45, 27, and 23 kDa were observed on the SDS-PAGE and identified as the expression gene products of the pmoB, pmoC, and pmoA, respectively. (ncl.edu.tw)
On June 24, the hospital laboratory reported an atypical gram-negative isolate from a blood specimen, which was sent that day to a commercial reference laboratory for further identification using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF). (cdc.gov)
Cell proteins were analysed with 2-dimensional electrophoresis and differentially expressed proteins were identified by matrix-assisted laser desorption/ ionization time-of-flight mass spectrometry. (who.int)
In October 2022, Zimbabwe made the Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS) technology available for the clinical microbiology laboratory's quick and precise identification of bacteria, mycobacteria, and fungal infections. (who.int)
Matrix-assisted laser29
Matrix-assisted laser desorption electrospray ionization (MALDESI) was first introduced in 2006 as a novel ambient ionization technique which combines the benefits of electrospray ionization (ESI) and matrix-assisted laser desorption/ionization (MALDI). (wikipedia.org)
Matrix-assisted laser desorption/ionization (MALDI) mass spectrometry (MS) can be used for such determination and its key advantage is the tolerance to salts, detergents and contaminants. (jove.com)
The following is a summary of the " Performance of lipid fingerprint by routine matrix-assisted laser desorption/ionization time of flight for the diagnosis of Mycobacterium tuberculosis complex species ," published in the March 2023 issue of Clinical Microbiology and Infections Disease by Gonzalo , et al. (physiciansweekly.com)
Heat-inactivated mycobacteria cultures were then loaded onto the matrix-assisted laser desorption/ionization target. (physiciansweekly.com)
Matrix-assisted laser desorption/ionization-time of flight had a sensitivity of 94.4 (95% CI, 86.4-98.5) and a specificity of 94.4 (95% CI, 72.7-99.9). (physiciansweekly.com)
This report presents an analytical approach to tissue characterization based on matrix-assisted laser desorption ionization time-of-flight (MALDI TOF) mass spectrometry imaging (MSI) which is introduced in an attempt to develop a reference database for predictive classification of brain tumors. (nih.gov)
Goodwin, R.J. , MacIntyre, L. , Watson, D.G. , Scullion, S.P. and Pitt, A.R. (2010) A solvent-free matrix application method for matrix-assisted laser desorption/ionization imaging of small molecules. (gla.ac.uk)
Matrix application continues to be a critical step in sample preparation for matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI). (gla.ac.uk)
This study examined whether matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS) could detect multiply charged dyes of pen inks directly from paper. (ojp.gov)
In the last decade, matrix-assisted laser desorption ionization TOF mass spectrometry (MALDI-TOF MS) was introduced as a modern microbiological diagnostic tool and has recently been used routinely as a powerful assay for rapid and highly accurate microbial identification in microbiology laboratories. (medscape.com)
Available applications and workflow of matrix-assisted laser desorption ionization TOF mass spectrometry using different clinical samples. (medscape.com)
Matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry, and its associated analytic software, was recently approved for clinical microbiology diagnostic use by the United States Food and Drug Administration. (temple.edu)
Meier, MAR, Lohmeijer, BGG & Schubert, US 2003, ' Relative binding strength of terpyridine model complexes under matrix-assisted laser desorption/ionization mass spectrometry conditions ', Journal of Mass Spectrometry , vol. 38, no. 5, pp. 510-516. (tue.nl)
Comparison of the newer techniques with the more established gas chromatography/mass spectrometry and attenuated total reflection Fourier transform infrared spectroscopic imaging shows good agreement between the methods, with each method detecting repeatable differences between the donors, with the exception of matrix-assisted laser desorption ionization, for which quantitative analysis has not yet been established. (northumbria.ac.uk)
Here, we present a matrix-assisted laser desorption/ionization imaging study which reports regio-specific changes in CL, in a controlled cortical impact model of TBI in rats. (cdc.gov)
Matrix-assisted laser desorption/ionization imaging revealed that TBI caused early decreases in CL in the contusional cortex, ipsilateral hippocampus, and thalamus with the most highly unsaturated CL species being most susceptible to loss. (cdc.gov)
Matrix-assisted Laser Desorption Ionization-Time of Flight Mass Spectroscopy (MALDI-TOF MS) has been used routinely over the past decade in clinical microbiology laboratories to rapidly characterize diverse microorganisms of medical importance both at the genus and species levels. (frontiersin.org)
Recently, we combined atmospheric pressure matrix assisted laser desorption (AP-MALDI) with a commercial ion trap mass spectrometer (ITMS) [1]. (nih.gov)
1. High-resolution matrix-assisted laser desorption/ionization imaging of tryptic peptides from tissue. (nih.gov)
2. Protein identification by accurate mass matrix-assisted laser desorption/ionization imaging of tryptic peptides. (nih.gov)
3. Matrix-assisted laser desorption/ionization imaging protocol for in situ characterization of tryptic peptide identity and distribution in formalin-fixed tissue. (nih.gov)
7. Chemically-assisted fragmentation combined with multi-dimensional liquid chromatography and matrix-assisted laser desorption/ionization post source decay, matrix-assisted laser desorption/ionization tandem time-of flight or matrix-assisted laser desorption/ionization tandem mass spectrometry for improved sequencing of tryptic peptides. (nih.gov)
9. Peptides quantification by liquid chromatography with matrix-assisted laser desorption/ionization and selected reaction monitoring detection. (nih.gov)
10. Dansyl-peptides matrix-assisted laser desorption/ionization mass spectrometric (MALDI-MS) and tandem mass spectrometric (MS/MS) features improve the liquid chromatography/MALDI-MS/MS analysis of the proteome. (nih.gov)
11. Comparison of vacuum matrix-assisted laser desorption/ionization (MALDI) and atmospheric pressure MALDI (AP-MALDI) tandem mass spectrometry of 2-dimensional separated and trypsin-digested glomerular proteins for database search derived identification. (nih.gov)
12. Capillary liquid chromatography/atmospheric-pressure matrix-assisted laser desorption/ionisation ion trap mass spectrometry: a comparison with liquid chromatography/matrix-assisted laser desorption/ionisation time-of-flight and liquid chromatography/electrospray ionisation quadrupole time-of-flight for the identification of tryptic peptides. (nih.gov)
16. De novo sequencing of tryptic peptides sulfonated by 4-sulfophenyl isothiocyanate for unambiguous protein identification using post-source decay matrix-assisted laser desorption/ionization mass spectrometry. (nih.gov)
This Matrix Assisted Laser Desorption Ionization - Time of Flight (MALDI-TOF) mass spectrometer was obtained in 2015. (edu.au)
Electrospray2
The electrospray post-ionization employed in IR-MALDESI helps alleviate issues associated with low ionization yield. (wikipedia.org)
In a study to improve the detection of tissue-specific lipids, the electrospray parameters have been tailored for positive and negative ionization polarities. (wikipedia.org)
Proteins1
Brzhozovskiy A, Kononikhin A, Bugrova AE, Kovalev GI, Schmit PO, Kruppa G, Nikolaev EN, Borchers CH . The Parallel Reaction Monitoring-Parallel Accumulation-Serial Fragmentation (prm-PASEF) Approach for Multiplexed Absolute Quantitation of Proteins in Human Plasma. (mcgill.ca)
Imaging1
Over the past ten years, transformative developments in the laser technology, data acquisition, motor-control software (RastirX), and imaging processing software (MSiReader) have promoted IR-MALDESI as a powerful tool for the direct analysis and MSI of diverse biological, forensic, and pharmaceutical samples. (wikipedia.org)
Molecular1
This study demonstrates that LDI FT-ICR MS is an effective ionization technique for use in the study of shale oils at the molecular level. (usgs.gov)
Compounds1
Previous work showed that LDI is a sensitive ionization technique for assessing aromatic nitrogen compounds, and oils generated from Green River Formation oil shales are well-documented as being rich in nitrogen. (usgs.gov)
Spectra1
Their method was quick (10 minutes), based on the identification of distinct lipid signatures from the seven reference strains, and yielded interpretable profiles for all but 4 isolates due to poor ionization, for a total of n = 86 with interpretable spectra. (physiciansweekly.com)
Liquid1
Ionisation modes available include ESI, APCI (liquid and direct probe), APPI (liquid and direct probe), online nanospray source (Captivespray nanoBooster source) and a home-built offline nanospray source. (edu.au)