• Development of a lab-on-a-chip method for rapid assay of Xylella fastidiosa subsp. (nature.com)
  • In this work, the two standard methods are compared with an innovative on-chip detection strategy for X. fastidiosa assay from leaves samples, based on an electrochemical transduction method. (nature.com)
  • Thus, the proposed technology has the potential to provide a useful assay method for large-scale monitoring programs. (nature.com)
  • 3. Detection assay for HPV16 and HPV18 by loop‑mediated isothermal amplification with lateral flow dipstick tests. (nih.gov)
  • 5. Rapid and sensitive detection of shrimp infectious myonecrosis virus using a reverse transcription loop-mediated isothermal amplification and visual colorogenic nanogold hybridization probe assay. (nih.gov)
  • 6. Reverse transcription loop-mediated isothermal amplification (RT-LAMP) combined with colorimetric gold nanoparticle (AuNP) probe assay for visual detection of Penaeus vannamei nodavirus (PvNV). (nih.gov)
  • 7. Isothermal Method of a Recombinase Polymerase Amplification Assay for the Detection of Most Common High-Risk Human Papillomavirus Type 16 and Type 18 DNA. (nih.gov)
  • The LAMP assay was more resilient than traditional qPCR to humic acid in sample, amplifying with up to 100mg per L of humic acid per reaction compared to 1mg per L for qPCR. (nih.gov)
  • Previously, we established the loop-mediated isothermal amplification (LAMP) method for a PCV13 serotype-specific assay. (wayne.edu)
  • In the current study, we developed a rapid, simple, and cost-effective assay to detect serotypes in the 23-valent pneumococcal polysaccharide vaccine (PPSV23) using the LAMP method. (wayne.edu)
  • The feasibility of LAMP assays in clinical application in patients with invasive pneumococcal diseases was validated by defining the detection limit of the LAMP assay with bacterial genomic DNA-spiked blood specimens. (wayne.edu)
  • The specificity of each LAMP assay was determined using 44 serotypes of pneumococcal strains. (wayne.edu)
  • In conclusion, a rapid and simple LAMP-based PPSV23-targeted serotype detection assay was developed for use in many countries. (wayne.edu)
  • This study is the first report of a LAMP-based assay for identification of PPSV23 serotypes. (wayne.edu)
  • CDC researchers have developed a simple loop-mediated isothermal amplification (LAMP)-based assay to detect this pathogen. (nih.gov)
  • This method only requires a simple heat block and is an easy-to-read colorimetric assay. (nih.gov)
  • The duplex tetra-primer ARMS-PCR assay described here can be applied to differentiate between Schistosoma species and their hybrid forms that infect humans and animals, thereby providing a method to investigate the epidemiology of these species in endemic areas. (biomedcentral.com)
  • ABSTRACT We evaluated the utility of 2 methods for detection of methicillin-resistant Staphylococcus aureus (MRSA) directly from signal-positive blood culture bottles: loop-mediated isothermal amplification (LAMP) assay, and direct cefoxitin disk diffusion (DCDD) test using a 30 μg cefoxitin disk. (who.int)
  • Conventional hospitals in Egypt to implement sound 1 mL aliquot was used directly for the methods for the identification of S. au- infection prevention and control pro- LAMP assay and DCDD test. (who.int)
  • Focusing on Mexico's most-fished species, Alexis hopes to test a method that is based on DNA and proteins called loop-mediated isothermal amplification (LAMP). (saveourseas.com)
  • The FDA recently authorized a different NAAT test method called loop-mediated isothermal amplification (LAMP). (nih.gov)
  • To ensure that the test will be as sensitive as possible, a specific fragment of the genetic material is amplified (copied) using the polymerase chain reaction method, known as PCR. (rivm.nl)
  • means a COVID-19 screening or diagnostic test carried out by an accredited laboratory, including a test performed using the method of polymerase chain reaction (PCR) or reverse transcription loop-mediated isothermal amplification (RT-LAMP). (canada.ca)
  • At present, most NAAT tests use an amplification method called polymerase chain reaction (PCR). (nih.gov)
  • The aim of this study was to utilize the quantitative real-time polymerase chain reaction (qPCR) method for direct quantitative detection of S. aureus in Turkish white cheese samples and identification of the isolated colonies using phenotypic and molecular methods. (iyte.edu.tr)
  • Current method of diagnosis involves observation, segregated cultivation and testing, and molecular biology techniques such as Polymerase Chain Reaction (PCR). (psu.ac.th)
  • Loop-mediated isothermal amplification (LAMP), a novel nucleic acid amplification method, was developed for the clinical detection of four species of human malaria parasites: Plasmodium falciparum, P. vivax, P. malariae, and P. ovale. (scienceopen.com)
  • 9. Α 2-stage, nested-like nucleic acid amplification method (IsoPCR) for the highly sensitive detection of HPV16 and HPV18 DNA. (nih.gov)
  • Did you know that this isothermal amplification method can be performed in as little as 5-10 minutes with limited resources? (neb.com)
  • Two surveys were conducted in Haiti during which we aimed to evaluate the performance of the simple and rapid procedure for ultra-rapid extraction-loop-mediated isothermal amplification (PURE-LAMP) method with dried blood spots as an alternative diagnostic method for malaria in the context of low to very low rates of transmission. (biomedcentral.com)
  • This study confirmed the high performance of the PURE-LAMP method to detect Plasmodium infection with dried blood spots and recommends its use in targeted mass screening and treatment activities in low endemic areas of malaria. (biomedcentral.com)
  • PCR methods to detect malaria infection were described more than two decades ago, and are generally more sensitive for detecting parasite DNA than thick BS is for detecting parasites. (medscape.com)
  • Molecular diagnostic methods can complement existing tools to improve the diagnosis of malaria. (scienceopen.com)
  • In this study, we attempted to improve this method for malaria diagnosis by using a simple and portable device capable of performing both the amplification and detection (by fluorescence) of LAMP in one platform. (scienceopen.com)
  • This RealAmp method has great potential as a field usable molecular tool for diagnosis of malaria. (scienceopen.com)
  • Armed with his training and expertise in entomology, biochemistry, molecular biology, and molecular genetics, Bill quickly demonstrated his talents by innovating methods used to detect and assess insecticide resistance in mosquitoes and other insects that spread diseases like malaria , dengue , and Zika . (cdc.gov)
  • For example, CDC is using Advanced Molecular Detection (AMD) methods to develop novel tools to detect and manage insecticide resistance in mosquitoes that spread diseases such as malaria, dengue, and Zika. (cdc.gov)
  • This method does not require DNA purification, utilizes simple instrumentation (water bath or a heat block), and can be completed in less than 1 h. [ 33 , 34 ] Several studies that compared the LAMP method to microscopy, PCR and RDT, reported high sensitivity and specificity compared with microscopy or PCR in nonpregnant populations. (medscape.com)
  • Clinical samples of P. falciparum were used to determine the sensitivity and specificity of this system compared to microscopy and a nested PCR method. (scienceopen.com)
  • The sensitivity and specificity of RealAmp in detecting P. falciparum was 96.7% and 91.7% respectively, compared to microscopy and 98.9% and 100% respectively, compared to a standard nested PCR method. (scienceopen.com)
  • We evaluated the sensitivity and specificity of LAMP in comparison with the results of microscopic examination and nested PCR. (scienceopen.com)
  • Of 60 blood cultures positive for Gram-positive cocci in clusters, LAMP (via detection of the FemA and MecA genes) showed 100% sensitivity and specificity for identification of MRSA/MSSA. (who.int)
  • By combination with reverse transcription, LAMP can amplify RNA sequences with high efficiency. (idtdna.com)
  • Over the years, he continued to advance the field with development of novel assays using loop mediated isothermal amplification (LAMP/RT-LAMP), a technique to amplify genetic material, with an eye towards assessing whether this method could be used for practical field surveillance and analysis of insecticide resistance in low resource, "real world" settings. (cdc.gov)
  • Their blood samples were tested by microscopy, rapid diagnostic tests (RDT), PURE-LAMP and nested PCR to detect Plasmodium infection. (biomedcentral.com)
  • Whether applied to peripheral blood or placental blood samples, PCR methods yield positivity rates 20% or more above those of BS microscopy ( Table 3 & Table 4 ). (medscape.com)
  • RT-LAMP can achieve high specificity due to the target sequences. (idtdna.com)
  • The reactivity, specificity, and sensitivity of LAMP assays were determined and compared to those of conventional PCR. (wayne.edu)
  • 1. High sensitivity, loop-mediated isothermal amplification combined with colorimetric gold-nanoparticle probes for visual detection of high risk human papillomavirus genotypes 16 and 18. (nih.gov)
  • 2. WarmStart colorimetric LAMP for the specific and rapid detection of HPV16 and HPV18 DNA. (nih.gov)
  • 4. [Colorimetric detection of HPV6 and HPV16 by loop mediated isothermal amplification]. (nih.gov)
  • Saliva samples with quick RNA extraction by heating and colorimetric LAMP are promising options for countries with economic and infrastructure limitations. (bvsalud.org)
  • It's not close to replacing RT-qPCR, which is still the gold standard in this field, but LAMP is evolving into a good alternative for rapid testing. (idtdna.com)
  • [ 29 , 30 ] qPCR and similar methods have become popular for early detection of parasites in vaccine trials and experimental human infections. (medscape.com)
  • Both PCR and qPCR methods have been applied to detect submicroscopic PM, and have been used as research tools in the context of clinical trials and epidemiological studies. (medscape.com)
  • Three separate field-able methods of biomass concentration with eight aquifer samples were also tested, comparing direct LAMP with traditional DNA extraction and quantitative PCR (qPCR). (nih.gov)
  • In the naturally contaminated cheese samples, the bacterial numbers calculated by the qPCR method were higher than the plate counts which can be related to detection of live and dead cells together. (iyte.edu.tr)
  • 4 . Background: The following questions are designed to better understand the testing methods that are used in the laboratory for molecular tests. (surveymonkey.com)
  • Since the 1980s, the use of molecular markers and associated analytical methods has been steadily increasing, not only within the research environment but also in fields such as human and environmental diagnostics. (biomedcentral.com)
  • This second edition consists of fourteen chapters detailing essential information on the basic principles of disease causation, major diseases of cultured fish and crustaceans, particularly shrimps/prawns, harmful algae, methods of prevention and control, molecular/serological/histological diagnostic techniques and epidemiology. (seafdec.org.ph)
  • The recently developed loop-mediated isothermal amplification (LAMP) method is relatively simple and can be improved for better use in endemic countries. (scienceopen.com)
  • Though new modern diagnostic methods have developed, GSBS is the most common diagnostic method in practice. (ijlr.org)
  • Lyo-ready Bst DNA polymerase provides maximum flexibility to optimize your LAMP reaction and work with various types of pathogens, including human adenovirus, measles, SARS-CoV-2, and other pathogens. (thermofisher.com)
  • The exceptional speed of lyo-ready DNA polymerase provides target detection in as few as ten minutes in a LAMP reaction. (thermofisher.com)
  • Lyo-ready Bst DNA polymerase demonstrates fast viral RNA amplification with detection in as few as ten minutes in an RT-LAMP reaction, using SSIV reverse transcriptase for the initial amplification step. (thermofisher.com)
  • Amplification speed was determined in RT-LAMP reaction using SARS-CoV-2 synthetic RNA and Measles viral RNA, their respective primer sets, and SYTO 9 green fluorescent nucleic acid stain for real-time detection. (thermofisher.com)
  • Lyo-ready Bst DNA Polymerase achieves sensitivity down to 50 copies in LAMP reaction. (thermofisher.com)
  • Lyo-ready Bst DNA Polymerase amplifies as low as 50 copies of DNA target in LAMP reaction. (thermofisher.com)
  • LAMP is simple and easy to perform once the appropriate primers are prepared, requiring only four primers, a DNA polymerase and a regular laboratory water bath or heat block for reaction," the authors wrote. (idtdna.com)
  • ulase-negative staphylococci (CoNS) need for a thermocycler, the speed of Approval to perform the study was and methicillin-susceptible strains has the reaction and visual read-out make obtained from the ethics committee in significant prognostic, therapeutic and LAMP a promising platform for the the Faculty of Medicine, Suez Canal economic value. (who.int)
  • Thus born A. hydrophila test kit, which uses Loop-mediated isothermal amplification-lateral-flow dipstick (LAMP-LFD) technique. (psu.ac.th)
  • 17. Genomagnetic LAMP-based electrochemical test for determination of high-risk HPV16 and HPV18 in clinical samples. (nih.gov)
  • LAMP DX utilizes loop mediated isothermal amplification (LAMP) which achieve highly accurate testing results from nose/throat swabs or saliva. (clearme.ca)
  • Little is known about how asymptomatic testing as a method to control transmission of COVID-19 can be implemented, and the prevalence of asymptomatic infection within university populations. (biomedcentral.com)
  • LAMP showed higher diagnostic accuracy although DCDD was more cost-effective and did not require additional reagents or supplies. (who.int)
  • Since the time of its introduction, the LAMP method has been usually used for the detection of infections in which just the amplification of the pathogen genome is required. (wcrj.net)
  • A workshop titled "Application of Genotyping Methods to Assess Pathogen Risks from Cryptosporidium in Drinking Water Catchments" was held at the International Water Association biennial conference, Marrakech, Morocco, 23 September 2004. (nih.gov)
  • Detection of four Plasmodium species by genus- and species-specific loop-mediated isothermal amplification for clinical diagnosis. (scienceopen.com)
  • Benchmarking bacterial genome-wide association study methods using simulated genomes and phenotypes. (cdc.gov)
  • For direct bacterial DNA extraction from cheese samples, four different methods were compared. (iyte.edu.tr)
  • Unlike previous studies, this work dives into the technical specifics of individual research papers and methodologies, ranging from basic to advanced control methods like computer vision and deep learning, while also assessing the energy consumption associated with each approach. (researchgate.net)
  • Amplification speed was determined in LAMP reactions using adenovirus AdV41 synthetic DNA and Mycoplasma pneumoniae genomic DNA, their respective primer sets and SYTO 9 green fluorescent nucleic acid stain for real-time detection. (thermofisher.com)
  • Regardless of the test or sampling method, at least two sites per suspicious lesion should be submitted to increase the sensitivity by 25% over a single specimen. (medscape.com)
  • Published genus-specific primers were used to test the utility of this method. (scienceopen.com)
  • The category of tests known as NAAT (Nucleic Acid Amplification Test) includes the PCR test, the TMA test and the LAMP test. (rivm.nl)
  • For a TMA (Transcription Mediated Amplification) test, a sample is taken using the same method as the PCR test, and then processed in a laboratory. (rivm.nl)
  • A TMA test uses a different measuring method than the PCR, expressed in Relative Light Units (RLU). (rivm.nl)
  • The LAMP (loop-mediated isothermal amplification) test is a form of testing in which small fragments of genetic material are copied or replicated, similar to the PCR test. (rivm.nl)
  • However, the method used for that purpose in the LAMP test is completely different from the PCR test. (rivm.nl)
  • The major advantage of the LAMP test is that the fragments of genetic material are replicated at only one temperature. (rivm.nl)
  • The LAMP test requires different equipment and chemical substances than are needed for PCR tests. (rivm.nl)
  • En parallèle, une identification microbiologique normalisée et un test de sensibilité à l'oxacilline par PCR visant l'amplification du gène MecA ont été réalisés. (who.int)
  • the use of an isothermal technology such as loop mediated isothermal amplification (LAMP) and naked eye test-result visualization by in-tube colorimetry or turbidity. (bvsalud.org)
  • Since the LAMP method is a sharp, fast and robust technique, it is suitable for use in routine diagnostics. (wcrj.net)
  • Modern outdoor lighting systems usually use LED lamps. (nvlightingga.com)
  • In fact, at Night Vision Outdoor Lighting, we use LED lamps exclusively. (nvlightingga.com)
  • The daisy chain is one of the most common methods of wiring an outdoor lighting system. (nvlightingga.com)
  • For most purposes, the daisy chain is the least effective method of wiring an outdoor lighting system. (nvlightingga.com)
  • Designing a lighting system with the potential for repositioning of the lamps is crucial because landscaping is prone to change over time. (nvlightingga.com)
  • Direct loop mediated isothermal amplification on filters for quantification of Dehalobacter in groundwater. (nih.gov)
  • This study expands previous work with direct loop mediated isothermal amplification (LAMP) for the detection and quantification of Dehalobacter spp. (nih.gov)
  • The detection range of S. aureus quantification was improved down to 13.2 CFU/g with the combined DNA extraction method of using trypsin and the food DNA extraction kit in artificially contaminated cheese samples. (iyte.edu.tr)
  • Similar methods rely on the turbidity of the sample, which increases with the amount of genetic material, to measure viral content. (idtdna.com)
  • Please note that every choice of a single error metric implies some kind of weighting between different quality aspects: Robustness (on how many datasets a SLAM method works) and accuracy (how well it works on those datasets where it does not fail). (eth3d.net)
  • As it is displaced, the end of the product forms a self-hybridizing loop structure, due to inclusion of a reverse complementary sequence in the inner primer sequence. (neb.com)
  • The Talis One's setup is similar to the Accula, except the machine uses reverse-transcription LAMP (RT-LAMP), which operates at a constant temperature of 65 degrees C, which means the equipment overall is much simpler. (idtdna.com)
  • These methods have a sensitivity similar to or better than conventional PCR. (medscape.com)
  • Additionally, directly boiled parasite preparations were compared with a conventional DNA isolation method. (scienceopen.com)
  • This tool can provide an alternative to conventional PCR based diagnostic methods for field use in clinical and operational programs. (scienceopen.com)
  • The realized lab-on-chip includes also a microfluidic module and its performances are competitive with conventional diagnostic methods in terms of reliability, but with further advantages of portability, low-costs and ease of use. (nature.com)
  • One modification that can significantly reduce voltage drop in a daisy chain is to run one more wire from the final fixture back to the transformer, creating a closed loop. (nvlightingga.com)
  • The main thing to watch out for in a loop setup is making sure that the polarity of the wires matches all the way through the loop and back to the transformer. (nvlightingga.com)
  • The GFX4-IR is managed by a microprocessor that independently controls the four control loops and also runs functions specifically designed to manage single-phase and 3-phase loads, with low and high-temperature coefficient, medium and short wave infrared lamps, and transformer primary circuits. (gobigchief.com)
  • Phase angle control with a current limit for short wave IR lamps, transformer primaries, with an assignment of soft start-up and soft stop down options with limitation of max. (gobigchief.com)
  • LAMP is fast, robust and less expensive than PCR and, subsequently, is applicable in less well-equipped laboratories although the current cost per sample is still higher than ideal. (biomedcentral.com)
  • The direct filter amplification (DFA) method eliminated an elution step and provided a detection limit of 102Dehalobacter cells per 100mL. (nih.gov)
  • LAMP is used for the detection of various pathogens, including bacteria, viruses, and protozoans that may cause food contamination and plant disease. (thermofisher.com)
  • LAMP is emerging as a new technology which permits faster, cheaper diagnostic testing for a range of diseases. (idtdna.com)
  • This has been particularly concerning as initial methods to control the virus had relied on isolation of people who were displaying symptoms. (biomedcentral.com)
  • One benefit touted by the manufacturers of LED lamps is that LED lamps can function with a range of voltages. (nvlightingga.com)
  • For a given error threshold on the x axis, the plots show for how many datasets a method achieves a lower error. (eth3d.net)
  • Four European Salmonella Typhimurium datasets collected to develop WGS-based source attribution methods. (cdc.gov)
  • Born out of 3 innovations - environmentally-friendly Curcuminoid extraction method, polymerize Curcuminoid extract with Hydroxyprobyl-beta-cyclodextrin and Polyvinylpyrolysed K30 to increase water solubility, and to transform Curcumoid extract into foam pellet, all of which enhance bioavailability of Curcuminoid when incorporated in food and medicinal products. (psu.ac.th)
  • The LAMP method is an Isothermal technique that amplifies the DNA segment of interest. (wcrj.net)
  • Loop-mediated isothermal amplification, also known as LAMP, is a simple and cost-effective nucleic acid amplification technique that does not require expensive instruments and helps ensure rapid results and easy outcome interpretation. (thermofisher.com)
  • It's a single-tube technique for amplifying DNA and, when used for disease detection, is cheaper than standard methods, which makes it suitable when large-scale testing is required. (idtdna.com)
  • Toxoplasma nucleic acid in CSF was detected using a loop-mediated isothermal amplification technique (LAMP) with previously validated methods ( 3 ). (cdc.gov)
  • When designing a LAMP experiment, four to six primers are required, specific to identified regions of target DNA or RNA sequence. (neb.com)
  • This LAMP dumbbell structure contains multiple sites for initiation of synthesis from the three prime ends of the open loops and annealing sites for both the inner and loop primers. (neb.com)
  • X. fastidiosa detection is usually performed by ELISA and PCR methods. (nature.com)
  • Detection of G119S ace-1R mutation in field-collected Anopheles gambiae mosquitoes using allele-specific loop-mediated isothermal amplification (AS-LAMP) method 14:47. (irac-online.org)
  • Today, Bill's methods continue to be used around the world to identify the ways mosquitoes become resistant to insecticides. (cdc.gov)
  • RT-LAMP is an emerging technology which uses auto-cycling strand displacement DNA synthesis as its basis. (idtdna.com)
  • NAAT tests are dependent upon a method used to multiply the relatively few copies of viral nucleic acid that might be present in a specimen into a very large number of copies - making it much easier detect the virus. (nih.gov)
  • The RealAmp method was found to be simple and allowed real-time detection of DNA amplification. (scienceopen.com)
  • 14. Rapid and sensitive detection of shrimp yellow head virus using loop-mediated isothermal amplification and a colorogenic nanogold hybridization probe. (nih.gov)
  • Alexis is keen to close the knowledge gap and assess the best method to identify shark products sold as fillets in Mexico. (saveourseas.com)
  • The primary goal of the trial and workshop was to assess the utility of current Cryptosporidium genotyping methods for determining the public health significance of oocysts isolated from feces in potable-water-supply watersheds. (nih.gov)
  • In addition, this method consistently detected P. falciparum from directly boiled blood samples. (scienceopen.com)
  • LAMP with crudely lysed Dehalobacter had a negligible effect on threshold time and sensitivity compared to lysed samples. (nih.gov)
  • Today, the NP and mid-nasal samples are the primary sampling methods used for antigen testing, but the development of antigen tests for saliva are underway. (nih.gov)
  • The single-strand conformational polymorphism method was considered potentially the most valuable for discriminating to the subtype level and where a large number of samples were to be analyzed. (nih.gov)
  • All of these methods require advanced skills, a lab, and specific tools. (psu.ac.th)
  • Unlike other technologies, RT-LAMP recognizes the target sequence using six independent sequences at the start and by four independent sequences towards the latter stages," reports Life Sciences News Medical . (idtdna.com)