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LamininReceptors, LamininBasement MembraneIntegrin alpha6beta1DystroglycansIntegrin alpha3beta1IntegrinsCell AdhesionIntegrin alpha6Extracellular MatrixCollagen Type IVLutheran Blood-Group SystemIntegrin alpha3Integrin alpha6beta4Integrin beta4Cell Adhesion MoleculesAntigens, CD29FibronectinsExtracellular Matrix ProteinsMuscular Dystrophy, AnimalHeparan Sulfate ProteoglycansEpidermolysis BullosaCollagenProtein IsoformsCell MovementAgrinSchwann CellsProtein BindingBruch MembraneCells, CulturedMuscular DystrophiesMolecular Sequence DataDesmosomesAmino Acid SequenceImmunohistochemistryMice, 129 StrainMembrane GlycoproteinsFluorescent Antibody Technique, IndirectMicroscopy, ElectronCell LineProtein Structure, TertiaryMorphogenesisTumor Cells, CulturedCell DifferentiationKeratinocytesGene Expression Regulation, DevelopmentalIn Situ HybridizationCytoskeletal ProteinsMice, KnockoutNeuromuscular JunctionEpithelial CellsRecombinant ProteinsEmbryo, NonmammalianReverse Transcriptase Polymerase Chain ReactionRNA, MessengerElectrophoresis, Polyacrylamide GelMuscle, SkeletalBinding SitesMicroscopy, FluorescenceRetinaFluorescent Antibody TechniqueMice, Inbred C57BLBlotting, Western
Cell surface rExtracellular matrixIntegrinHeparan sulfateIntegrinsAlpha4FibronectinRegulateSubunitChainsBindBinding proteinMolecular weightCellularPotentiallyKEGGGeneCellsSmallFunctionExtracellularRPSACollagenHematopoiesisMultifunctionalPathwaysNeurite outgrowthAdhesionStructuresMigrationStemCellRoleExpressionHighlyEssentialFound
Cell surface r1
  • Many of the effects of laminin are mediated through interactions with cell surface receptors. (wikipedia.org)
Extracellular matrix2
  • Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. (wikipedia.org)
  • KEGG pathways enrichment and PPI network indicate four major pathways, including extracellular matrix -receptor interaction, focal adhesion, phosphatidylinositol 3-kinase (PI3K)-Protein kinase B (Akt), and Ras signaling pathways were involved in the degeneration of cartilage. (nature.com)
Integrin6
  • These receptors include members of the integrin family, as well as non-integrin laminin-binding proteins. (wikipedia.org)
  • The RPSA gene encodes a multifunctional protein, which is both a ribosomal protein and a high-affinity, non-integrin laminin receptor. (wikipedia.org)
  • Laminins are alphabetagamma heterotrimeric extracellular proteins that regulate cellular functions by adhesion to integrin and nonintegrin receptors. (ox.ac.uk)
  • Adhesion of CD34(+)CD38(-) cells to laminin-10/11 was maximal without integrin activation, whereas adhesion to other proteins was dependent on protein kinase C activation by 12-tetradecanoyl phorbol-13-acetate (TPA). (ox.ac.uk)
  • Integrin alpha6 and beta1 chains were involved in binding of both cell fractions to laminin-10/11 and laminin-8. (ox.ac.uk)
  • In functional assays, both laminin-8 and laminin-10/11 facilitated stromal-derived factor-1alpha (SDF-1alpha)-stimulated transmigration of CD34(+) cells, by an integrin alpha6 receptor-mediated mechanism. (ox.ac.uk)
Heparan sulfate2
Integrins2
  • The laminins can self-assemble, bind to other matrix macromolecules, and have unique and shared cell interactions mediated by integrins, dystroglycan, and other receptors. (embl.de)
  • Moreover, integrins, laminins, NF-κB and other regulative molecules were found as crucial proteins. (nature.com)
Alpha42
  • Laminin IV domain is also found in the perlecan protein, an integral component of basement membranes, which serves also as an attachment substrate for cells, but it is not found in short laminin chains (alpha4 or beta3). (embl.de)
  • Laminins containing alpha4 and alpha5 chains are expressed in bone marrow, but their interactions with hematopoietic progenitors are unknown. (ox.ac.uk)
Fibronectin3
  • We studied human bone marrow cell adhesion to laminin-10/11 (alpha5beta1gamma1/alpha5beta2gamma1), laminin-8 (alpha4beta1gamma1), laminin-1 (alpha1beta1gamma1), and fibronectin. (ox.ac.uk)
  • About 35% to 40% of CD34(+) and CD34(+)CD38(-) stem and progenitor cells adhered to laminin-10/11, and 45% to 50% adhered to fibronectin, whereas they adhered less to laminin-8 and laminin-1. (ox.ac.uk)
  • Whereas endothelial cells plated on fibronectin or fibrinogen activate NF-κB in response to flow, cells on collagen or laminin do not. (rupress.org)
Regulate2
  • We further found that the pericytic laminin isoforms differentially regulate mouse β-cells. (nature.com)
  • dGLYAT was found to regulate Gadd45 -mediated JNK pathway activation and cell invasion. (sdbonline.org)
Subunit1
  • ii) Serial deletions of RPSA have shown that the segment of residues 236-262, included in the C-domain, is involved in the interaction between RPSA and the 40S subunit of ribosome. (wikipedia.org)
Chains2
  • The different laminin chains share a 600-residue domain I/II which oligomerises into a rod-like coiled-coil structure forming the long arm of laminins. (embl.de)
  • Compared to ECs, pancreatic pericytes produce significantly higher levels of α2 and α4 laminin chains, which constitute the peri-islet and vascular BM. (nature.com)
Bind2
  • Both recombinant N-domain and C-domain of RPSA bind laminin in vitro, and they bind with similar dissociation constants (300 nM). (wikipedia.org)
  • Cytokines bind to their receptors, activating signal transduction pathways such as adenylate cyclase/cAMP, phospholipase C/inositol trisphosphate, and Ca 2+ and tyrosine kinases. (biomedcentral.com)
Binding protein1
Molecular weight1
  • Laminin is a large molecular weight glycoprotein present only in basement membranes in almost every animal tissue. (embl.de)
Cellular2
  • The folding intermediate is predominant at 37 °C. Several interactions of RPSA that had originally been discovered by methods of cellular biology, have subsequently been confirmed by using recombinant derivatives and in vitro experiments. (wikipedia.org)
  • RPSA is a potential cellular receptor for several pathogenic Flaviviruses, including the dengue virus (DENV), and Alphaviruses, including the Sindbis virus (SINV). (wikipedia.org)
Potentially1
  • Moreover, the C-domain of RPSA and heparin compete for binding to laminin, which shows that the highly acidic C-domain of RPSA mimicks heparin (and potentially heparan sulfates) for the binding to laminin. (wikipedia.org)
KEGG1
  • Proteomic results were analyzed with gene ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), and protein-protein interactions (PPI). (nature.com)
Gene1
  • Whereas α2 laminins promoted islet cell clustering, they did not affect gene expression. (nature.com)
Cells5
  • However, while many studies demonstrated the importance of the ECM, its interactions with β-cells are still not fully understood. (nature.com)
  • The interactions with the various BM components differentially affect β-cells, although the underlying mechanism is largely unknown. (nature.com)
  • Laminin isoform-specific promotion of adhesion and migration of human bone marrow progenitor cells. (ox.ac.uk)
  • Laminin-10/11 was highly adhesive to lineage-committed myelomonocytic and erythroid progenitor cells and most lymphoid and myeloid cell lines studied, whereas laminin-8 was less adhesive. (ox.ac.uk)
  • In conclusion, we demonstrate laminin isoform-specific adhesive interactions with human bone marrow stem, progenitor, and more differentiated cells. (ox.ac.uk)
Small1
Function2
  • RPSA is a ribosomal protein which has acquired the function of laminin receptor during evolution. (wikipedia.org)
  • This study demonstrated that the Receptor for Activated C-Kinase 1 (RACK1) is important in SC function. (sdbonline.org)
Extracellular4
  • Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. (wikipedia.org)
  • Neurite outgrowth is a key event in the differentiation of neuronal cells, and is regulated by extracellular environment factors such as nerve growth factor (NGF) and cell adhesion molecules such as laminins. (grantome.com)
  • Perl interacts with many extracellular molecules and cell surface receptors, and is implicated in many biological functions in tissue homeostasis and diseases. (grantome.com)
  • We developed an on-slide decellularization approach to generate acellular extracellular matrix (ECM) myoscaffolds that can be repopulated with various cell types to interrogate cell-ECM interactions. (nature.com)
RPSA8
  • RPSA is a ribosomal protein which has acquired the function of laminin receptor during evolution. (wikipedia.org)
  • The folding intermediate is predominant at 37 °C. Several interactions of RPSA that had originally been discovered by methods of cellular biology, have subsequently been confirmed by using recombinant derivatives and in vitro experiments. (wikipedia.org)
  • RPSA was initially identified as a laminin binding protein. (wikipedia.org)
  • Both recombinant N-domain and C-domain of RPSA bind laminin in vitro, and they bind with similar dissociation constants (300 nM). (wikipedia.org)
  • Both RPSA and laminin belong to the heparin/heparan sulfate interactome. (wikipedia.org)
  • Moreover, the C-domain of RPSA and heparin compete for binding to laminin, which shows that the highly acidic C-domain of RPSA mimicks heparin (and potentially heparan sulfates) for the binding to laminin. (wikipedia.org)
  • RPSA is a potential cellular receptor for several pathogenic Flaviviruses, including the dengue virus (DENV), and Alphaviruses, including the Sindbis virus (SINV). (wikipedia.org)
  • RPSA is also a receptor for small molecules. (wikipedia.org)
Collagen3
  • Basement membranes consist of laminin, collagen IV, perlecan, nidogen/entactin, and other molecules, which interact with one another to form the supramolecular structure. (grantome.com)
  • Proteomics and structural analysis revealed that excessive collagen deposition alone is not pathological, and can be compensatory, as revealed by overexpression of sarcospan and its associated ECM receptors in dystrophic muscle. (nature.com)
  • We further observe a regulatory role of the molecule in pathways associated with PDAC ECM remodeling and tumor-stromal crosstalk, such as INS/IGF-1, RAS/MAPK, laminin interactions and collagen biosynthesis. (biomedcentral.com)
Hematopoiesis1
  • The cell-adhesive laminins affected migration of hematopoietic progenitors, suggesting a physiologic role for laminins during hematopoiesis. (lu.se)
Multifunctional1
  • Laminins are multifunctional trimeric glycoproteins. (grantome.com)
Pathways1
  • Together, our study presents a comprehensive understanding of miR-29a regulation of PSCs, and identifies essential pathways associated with PSC-mediated PDAC pathogenesis. (biomedcentral.com)
Neurite outgrowth1
  • However, the role of GM1 in laminin-1-induced neurite outgrowth is not clear. (grantome.com)
Adhesion1
  • We studied human bone marrow cell adhesion to laminin-10/11 (alpha5beta1gamma1/alpha5beta2gamma1), laminin-8 (alpha4beta1gamma1), laminin-1 (alpha1beta1gamma1), and fibronectin. (lu.se)
Structures2
  • The cell structures that mediate those interactions between the cells and their ECM are one of the most amazing examples of functional complexity in the cell. (uncommondescent.com)
  • Laminin isoforms show tissue- and developmental stage-specific expression and play important roles in the structures and functions of basement membranes. (grantome.com)
Migration1
  • All SPMC interactions with fibrotic myoscaffolds from dystrophic muscle were severely blunted including reduced motility rate and migration. (nature.com)
Stem2
  • About 35% to 40% of CD34(+) and CD34(+)CD38(-) stem and progenitor cells adhered to laminin-10/11, and 45% to 50% adhered to fibronectin, whereas they adhered less to laminin-8 and laminin-1. (lu.se)
  • In conclusion, we demonstrate laminin isoform-specific adhesive interactions with human bone marrow stem, progenitor, and more differentiated cells. (lu.se)
Cell7
  • It also acts as a cell surface receptor, in particular for laminin, and is involved in several pathogenic processes. (wikipedia.org)
  • Many of the effects of laminin are mediated through interactions with cell surface receptors. (wikipedia.org)
  • It has been observed that the level of the laminin receptor transcript is higher in colon carcinoma tissue and lung cancer cell line than their normal counterparts. (nih.gov)
  • Laminin-10/11 was highly adhesive to lineage-committed myelomonocytic and erythroid progenitor cells and most lymphoid and myeloid cell lines studied, whereas laminin-8 was less adhesive. (lu.se)
  • All cell migrations happen in the ECM, and are controlled and implemented by the complex interactions between the cells and the ECM. (uncommondescent.com)
  • Those interactions, surprisingly, are not only biochemical (as we usually imagine cell interactions to be), but mainly mechanical. (uncommondescent.com)
  • Expression is found on epithelial cells and tumor cells and has been shown to be involved in cell-matrix and cell-cell interactions. (thermofisher.com)
Role1
  • however, the role of laminins in BBB development remains unclear. (nih.gov)
Expression1
  • Laminin-211 appears to mediate these effects at least in part by dystroglycan receptor interactions, as preventing dystroglycan expression in neural cells led to a similar set of BBB abnormalities and gliovascular disturbances, which additionally included perturbed vascular endothelial glucose transporter-1 localization. (nih.gov)
Highly1
Essential1
  • The interactions of laminins with cells are essential for laminin-mediated biological activities in tissue development and adult tissue functions. (grantome.com)
Found1
  • In collaboration with Dr. Eri Arikawa-Hirasawa's group, we found that laminin-1 bound to GM1 through a carbohydrate moiety and dependent on the conformation of GM1, it induced focal formation of large clusters of GM1, and it enhanced relocation of NGF receptor TrkA into the lipid rafts of dorsal root ganglion (DRG) and PC12 cells. (grantome.com)