Extracellular Matrix PIsoformsCollagensEpithelial cell adhesionProteoglycansFibronectinMembranesGlycoproteinStructuralIntegrinBasement membraneHumansOrgansAffinityAssaysHigh molecularChainsCongenitalBindPeptideCriticallyGenePeripheralGamma-chainArraysSubstrateBiologicalDomainFamilyFormMouseSignificantlyCommonUniqueNerveImportantFunctionNoteDomainsSMARTFunctionsShape
Extracellular Matrix P1
- Affinity for these extracellular matrix proteins explains the striking ability of NP41 to highlight degenerated nerve "ghosts" months posttransection that are invisible to the unaided eye but retain hollow laminin-rich tubular structures. (dualsystems.com)
Isoforms5
- To analyze alpha-integrin mediated adhesion of human corneal epithelial cells to placental and EHS laminin isoforms. (nih.gov)
- Using the human corneal epithelial cell line HCE-T, adhesion to laminin isoforms and fibronectin was determined using a colorimetric adhesion assay. (nih.gov)
- alpha-integrin sub-unit modulation of corneal epithelial cell interaction with laminin isoforms was analyzed using immunofluorescence microscopy and adhesion assays incorporating functional blocking antibodies. (nih.gov)
- These studies demonstrate that human corneal epithelial cells are capable of rapid adhesion to, and enhanced spreading on, laminin isoforms not characteristically resident in the adult corneal basement membrane. (nih.gov)
- We further found that the pericytic laminin isoforms differentially regulate mouse β-cells. (nature.com)
Collagens2
- There are a number of families of extracellular matrix (ECM) proteins including collagens, proteoglycans and laminins (LM). (urosario.edu.co)
- 10 −7 ) and minor enrichment of laminin-γ1 and collagens I and VI. (dualsystems.com)
Epithelial cell adhesion1
- Abnormal laminin-332, which is essential for epithelial cell adhesion to the basement membrane, leads to a condition called junctional epidermolysis bullosa, characterized by generalized blisters, exuberant granulation tissue of skin and mucosa, and pitted teeth. (wikipedia.org)
Proteoglycans2
- Other than water, the mesoglea is composed of several substances including fibrous proteins like collagen and heparan sulphate proteoglycans. (pearltrees.com)
- BMs are specialized structures that underlie epithelia and surround organs and mainly comprise collagen IV, laminins, and heparan sulfate proteoglycans (HSPG). (nature.com)
Fibronectin2
- Laminins form independent networks and are associated with type IV collagen networks via entactin, fibronectin, and perlecan. (wikipedia.org)
- For example, growth cones are repelled by netrin when grown on laminin-111 but are attracted to netrin when grown on fibronectin. (wikipedia.org)
Membranes6
- The proteins also bind to cell membranes through integrins and other plasma membrane molecules, such as the dystroglycan glycoprotein complex and Lutheran blood group glycoprotein. (wikipedia.org)
- These proteins have several short arms that bind easily to other laminins and a single long arm that binds easily to other cell structures and membranes. (gotquestions.org)
- He is known for his work on laminins - huge proteins that are a key component of all basement membranes. (imperial.ac.uk)
- Laminins [ ( PUBMED:2404817 ) ] are the major noncollagenous components of basement membranes that mediate cell adhesion, growth migration, and differentiation. (embl.de)
- Laminin is a large molecular weight glycoprotein present only in basement membranes in almost every animal tissue. (embl.de)
- Laminin IV domain is also found in the perlecan protein, an integral component of basement membranes, which serves also as an attachment substrate for cells, but it is not found in short laminin chains (alpha4 or beta3). (embl.de)
Glycoprotein2
- Laminin-421 and -211 were identified by proximity-based labeling using singlet oxygen and by an adapted version of TRICEPS-based ligand-receptor capture to identify glycoprotein receptors via ligand cross-linking. (dualsystems.com)
- Glycoprotein receptor capture also identified laminin-α4 and -γ1. (dualsystems.com)
Structural3
- This review critically focuses on opportunities to employ protein-graphene oxide structures either as nanocomposites or as biocomplexes and highlights the effects of carbonaceous nanostructures on protein conformation and structural stability for applications in tissue engineering and regenerative medicine. (mdpi.com)
- Laminins are integral to the structural scaffolding of almost every tissue of an organism-secreted and incorporated into cell-associated extracellular matrices. (wikipedia.org)
- 3] They are composed of a family of related proteins sharing common structural and sequence features. (pearltrees.com)
Integrin3
- Neurons of the peripheral nervous system express integrin receptors that attach to laminins and promote neuroregeneration after injury. (wikipedia.org)
- Immunofluorescence microscopy reveals that alpha-integrin protein localization patterns are not significantly different in HCE-T interacting with EHS or placental laminin. (nih.gov)
- However, in short-term assays alpha3 integrin plays a major role, and alpha2 integrin a minor role, in mediating HCE-T adhesion to laminin. (nih.gov)
Basement membrane2
- They are major constituents of the basement membrane, namely the basal lamina (the protein network foundation for most cells and organs). (wikipedia.org)
- They are a major component of the basal lamina (one of the layers of the basement membrane), a protein network foundation for most cells and organs. (pearltrees.com)
Humans3
- Laminins are heterotrimeric proteins with a high molecular mass (~400 to ~900 kDa) and possess three different chains (α, β and γ) encoded by five, four, and three paralogous genes in humans, respectively. (wikipedia.org)
- In humans, fifteen laminin trimers have been identified. (wikipedia.org)
- Thrombospondin 1, abbreviated as THBS1, is a protein that in humans is encoded by the THBS1 gene. (pearltrees.com)
Organs2
- Due to their large surface area and ability to interact with proteins and peptides, graphene oxides offer valuable physiochemical and biological features for biomedical applications and have been successfully employed for optimizing scaffold architectures for a wide range of organs, from the skin to cardiac tissue. (mdpi.com)
- This makes laminin a critical means of holding tissues and organs together. (gotquestions.org)
Affinity1
- The three short arms have an affinity for binding to other laminin molecules, conducing sheet formation. (wikipedia.org)
Assays2
- In short-term adhesion assays, the preferred substrate for HCE-T attachment is placental laminin. (nih.gov)
- Binding assays with phage expressing NP41 confirmed binding to purified laminin-421, laminin-211, and laminin-α4. (dualsystems.com)
High molecular1
- An Illustration of the Laminin-111 complex depicting the domain organization Laminins are high-molecular weight (~400 to ~900 kDa) proteins of the extracellular matrix. (pearltrees.com)
Chains5
- The laminin molecules are named according to their chain composition, e.g. laminin-511 contains α5, β1, and γ1 chains. (wikipedia.org)
- The laminins are combinations of different alpha-, beta-, and gamma-chains. (wikipedia.org)
- Laminin-211 is composed of an α2, β1 and γ1 chains. (wikipedia.org)
- Compared to ECs, pancreatic pericytes produce significantly higher levels of α2 and α4 laminin chains, which constitute the peri-islet and vascular BM. (nature.com)
- The different laminin chains share a 600-residue domain I/II which oligomerises into a rod-like coiled-coil structure forming the long arm of laminins. (embl.de)
Congenital1
- Dysfunctional structure of one particular laminin, laminin-211, is the cause of one form of congenital muscular dystrophy. (wikipedia.org)
Bind3
- The trimeric proteins intersect, composing a cruciform structure that is able to bind to other molecules of the extracellular matrix and cell membrane. (wikipedia.org)
- The most important property of laminins is their ability to easily bind to each other and to other proteins. (gotquestions.org)
- The laminins can self-assemble, bind to other matrix macromolecules, and have unique and shared cell interactions mediated by integrins, dystroglycan, and other receptors. (embl.de)
Peptide1
- For example, the peptide sequence [GTFALRGDNGDNGQ], which is located on the alpha-chain of laminin, promotes the adhesion of endothelial cells. (wikipedia.org)
Critically1
- Through these interactions, laminins critically contribute to cell attachment and differentiation, cell shape and movement, maintenance of tissue phenotype, and promotion of tissue survival. (wikipedia.org)
Gene1
- Whereas α2 laminins promoted islet cell clustering, they did not affect gene expression. (nature.com)
Peripheral1
- citation needed] Laminins are enriched at the lesion site after peripheral nerve injury and are secreted by Schwann cells. (wikipedia.org)
Gamma-chain1
Arrays1
Substrate1
- Laminin-111 is a major substrate along which nerve axons will grow, both in vivo and in vitro. (wikipedia.org)
Biological2
- Some of these biological functions of laminin have been associated with specific amino-acid sequences or fragments of laminin. (wikipedia.org)
- Or, could one claim laminin looks like a pitchfork and use that correlation as a biological proof of our inherited sin nature? (gotquestions.org)
Domain7
- The structure of the laminin-G domain has been predicted to resemble that of pentraxin. (wikipedia.org)
- It is known as a 'LE' or 'laminin-type EGF-like' domain. (embl.de)
- Taxonomic distribution of proteins containing EGF_Lam domain. (embl.de)
- The complete taxonomic breakdown of all proteins with EGF_Lam domain is also avaliable . (embl.de)
- Click on the protein counts, or double click on taxonomic names to display all proteins containing EGF_Lam domain in the selected taxonomic class. (embl.de)
- Percentage points are related to the number of proteins with EGF_Lam domain which could be assigned to a KEGG orthologous group, and not all proteins containing EGF_Lam domain. (embl.de)
- Taxonomic distribution of proteins containing LamB domain. (embl.de)
Family2
- Laminins are a family of glycoproteins of the extracellular matrix of all animals. (wikipedia.org)
- Laminin is the name used for a family of proteins that serve many useful functions in biology. (gotquestions.org)
Form2
- defective laminins can cause muscles to form improperly, leading to a form of muscular dystrophy, lethal skin blistering disease (junctional epidermolysis bullosa), and/or defects of the kidney filter (nephrotic syndrome). (wikipedia.org)
- Flattened out, laminins have a shape generally in the form of a lower case t , which some have compared to the shape of a cross. (gotquestions.org)
Mouse1
Significantly1
- In contrast, culturing on Laminin-421 induced the expression of β-cell genes, including Ins1 , MafA , and Glut2 , and significantly improved glucose-stimulated insulin secretion. (nature.com)
Common1
- In addition, many laminins had common names before either laminin nomenclature was in place. (wikipedia.org)
Unique1
- From a practical standpoint, the shape of laminin is neither unique nor extraordinarily similar to a cross. (gotquestions.org)
Nerve1
- Laminins colocalized with NP41 within nerve sheath, particularly perineurium, where laminin-421 is predominant. (dualsystems.com)
Important1
- The laminins are an important and biologically active part of the basal lamina, influencing cell differentiation, migration, and adhesion. (pearltrees.com)
Function1
- Such conclusions are completely unrelated to the shape, function, or purpose of laminin. (gotquestions.org)
Note1
- Please note that proteins can be included in multiple pathways, ie. (embl.de)
Domains2
SMART1
- This information is based on mapping of SMART genomic protein database to KEGG orthologous groups. (embl.de)
Functions1
- It has been described as the protein equivalent of glue, though it functions differently than actual chemical glue will. (gotquestions.org)
Shape2
- Is there any significance to laminin being in the shape of a cross? (gotquestions.org)
- The fact that laminin, a binding protein, has a shape vaguely similar to a cross is an interesting quirk of biology. (gotquestions.org)