• Elevated expression of laminins (LN) and integrins in the synovial membrane of rheumatoid arthritis (RA) versus osteoarthritis patients has been reported but metabolic effects of attachment of synovial fibroblasts (SF) to LN are not well studied. (biomedcentral.com)
  • This gene encodes the alpha 2 chain, which constitutes one of the subunits of laminin 2 (merosin) and laminin 4 (s-merosin). (wikipedia.org)
  • To analyze alpha-integrin mediated adhesion of human corneal epithelial cells to placental and EHS laminin isoforms. (nih.gov)
  • alpha-integrin sub-unit modulation of corneal epithelial cell interaction with laminin isoforms was analyzed using immunofluorescence microscopy and adhesion assays incorporating functional blocking antibodies. (nih.gov)
  • Immunofluorescence microscopy reveals that alpha-integrin protein localization patterns are not significantly different in HCE-T interacting with EHS or placental laminin. (nih.gov)
  • However, in short-term assays alpha3 integrin plays a major role, and alpha2 integrin a minor role, in mediating HCE-T adhesion to laminin. (nih.gov)
  • Expression of the α1, β1 and γ1 chains of LN1 laminin by SF was investigated by quantitative RT-PCR and immunocytochemistry. (biomedcentral.com)
  • In short-term adhesion assays, the preferred substrate for HCE-T attachment is placental laminin. (nih.gov)
  • Binding assays with phage expressing NP41 confirmed binding to purified laminin-421, laminin-211, and laminin-α4. (dualsystems.com)
  • Laminin subunit alpha-2 is a protein that in humans is encoded by the LAMA2 gene. (wikipedia.org)
  • Western blot analysis was used to partially characterize commercially available preparations of laminin isolated from the mouse EHS sarcoma and from human placenta. (nih.gov)
  • Figure 4: NMR analysis of binding of oligosaccharide G5 to laminin-α2 LG4-5. (nature.com)
  • 10 −7 ) and minor enrichment of laminin-γ1 and collagens I and VI. (dualsystems.com)
  • The following product was used in this experiment: CD104 (Integrin beta 4) Monoclonal Antibody (439-9B), eBioscience™ from Thermo Fisher Scientific, catalog # 14-1049-82, RRID AB_1210460. (thermofisher.com)
  • Description: The monoclonal antibody 439-9B recognizes human CD104, also known as integrin beta 4. (thermofisher.com)
  • The monoclonal antibody 439-9B has been reported to have no effect on adhesion of human colon adenocarcinoma cells. (thermofisher.com)
  • Reactivity of laminin α4 chain specific monoclonal antibody 6C3 on human platelet lysate by Western blotting (reducing, R and nonreducing, NR conditions). (icosagen.com)
  • This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated. (thermofisher.com)
  • Laminins are a family of large heterotrimeric proteins that promote cell adhesion and migration via integrins and other cell-surface receptors. (icosagen.com)
  • Laminin isoforms and their integrin receptors in glioma cell migration and invasiveness: Evidence for a role of alpha5-laminin(s) and alpha3beta1 integrin. (icosagen.com)
  • This gene encodes the alpha 2 chain, which constitutes one of the subunits of laminin 2 (merosin) and laminin 4 (s-merosin). (wikipedia.org)
  • Applications Reported: This 439-9B antibody has been reported for use in flow cytometric analysis, immunoprecipitation, immunoblotting under nonreducing conditions only, immunohistology staining of frozen tissue sections, and immunohistology staining of paraffin embedded tissue sections. (thermofisher.com)
  • Applications Tested: This 439-9B antibody has been tested by flow cytometric analysis of A549 cell line. (thermofisher.com)
  • A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. (thermofisher.com)
  • Laminin subunit alpha-2 is a protein that in humans is encoded by the LAMA2 gene. (wikipedia.org)