• Increased mRNA expression of a laminin-binding protein in human colon carcinoma: complete sequence of a full-length cDNA encoding the protein. (joplink.net)
  • The names 'acquired junctional epidermolysis bullosa', 'anti-laminin-332 mucous membrane pemphigoid (MMP)' and 'mixed auto-immune subepidermal blistering dermatosis' are proposed for dogs with clinical signs reminiscent of EBA, MMP or BP respectively. (morulaivf.com)
  • Laminin proteins are made of three different subunits called alpha, beta, and gamma. (medlineplus.gov)
  • Each laminin is a heterotrimer assembled from alpha, beta and gamma chain subunits, secreted and incorporated into cell-associated extracellular matrices. (embl.de)
  • LS-F33142 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Human LAMC2 / Laminin Gamma 2 in samples of Plasma, Serum and Tissue Homogenates. (lsbio.com)
  • It is based upon a Sandwich assay principle and can be used to detect levels of LAMC2 / Laminin Gamma 2 as low as 0.094 nanograms per millilter. (lsbio.com)
  • laminin subunit gamma 3 [Sour. (gsea-msigdb.org)
  • Laminin-332 (laminin-5) is a basement membrane heterotrimeric protein composed of alpha-3, beta-3 and gamma-2 laminin chains. (morulaivf.com)
  • Laminin -332 (Ln-332) is a heterotrimeric glycoprotein (alpha3beta3gamma2) unique to epithelial cells with crucial roles in signaling, adhesion, and migration. (bvsalud.org)
  • Laminin is a large molecular weight glycoprotein present only in basement membranes in almost every animal tissue. (embl.de)
  • Using the human corneal epithelial cell line HCE-T, adhesion to laminin isoforms and fibronectin was determined using a colorimetric adhesion assay. (nih.gov)
  • Polyclonal and monoclonal antibodies raised against chicken gizzard 5′-nucleotidase were tested in adhesion assays of embryonic chicken fibroblasts (CEF) for their ability to interfere with the adhesion process of these cells on either laminin or fibronectin substrata. (morulaivf.com)
  • The initial attachment process of CEF on fibronectin and laminin substrata was not influenced by preincubating these cells with antibodies against chicken gizzard 5′-nucleotidase. (morulaivf.com)
  • A decreased ratio of laminin-332 beta3 to gamma2 subunit mRNA is associated with poor prognosis in colon cancer. (bvsalud.org)
  • Laminin IV domain is also found in the perlecan protein, an integral component of basement membranes, which serves also as an attachment substrate for cells, but it is not found in short laminin chains (alpha4 or beta3). (embl.de)
  • Laminins [ ( PUBMED:2404817 ) ] are the major noncollagenous components of basement membranes that mediate cell adhesion, growth migration, and differentiation. (embl.de)
  • These studies demonstrate that human corneal epithelial cells are capable of rapid adhesion to, and enhanced spreading on, laminin isoforms not characteristically resident in the adult corneal basement membrane. (nih.gov)
  • Laminin and type IV collagen are the two principal components of the basement membrane. (biogenex.com)
  • Because type IV collagen and laminin are proteins found exclusively in the basement membrane, the antibodies to these two proteins selectively and specifically recognize basement membrane components. (biogenex.com)
  • Laminin consists of a 220 kD subunit, which is disulfide-linked into larger complexes or even into the structural scaffolding of the basement membrane itself. (biogenex.com)
  • In surgical pathology, laminin can be used as a marker to demonstrate morphologic change of basement membrane, which is helpful for interpreting the invasion of malignant tumors. (biogenex.com)
  • In five dogs, IgG variably recognized the basement membrane of laminin-332-deficient human skin (three dogs negative, two dogs positive). (morulaivf.com)
  • These observations establish laminin-332 as a novel basement membrane antigen in dogs with autoimmune blistering diseases with variable clinical phenotypes. (morulaivf.com)
  • To analyze alpha-integrin mediated adhesion of human corneal epithelial cells to placental and EHS laminin isoforms. (nih.gov)
  • alpha-integrin sub-unit modulation of corneal epithelial cell interaction with laminin isoforms was analyzed using immunofluorescence microscopy and adhesion assays incorporating functional blocking antibodies. (nih.gov)
  • Immunofluorescence microscopy reveals that alpha-integrin protein localization patterns are not significantly different in HCE-T interacting with EHS or placental laminin. (nih.gov)
  • However, in short-term assays alpha3 integrin plays a major role, and alpha2 integrin a minor role, in mediating HCE-T adhesion to laminin. (nih.gov)
  • The Integrin alpha 6 subunit, also known as CD49f and VLA-6 alpha subunit, forms a heterodimer with Integrin beta 1 (CD29) or beta 4 (CD104) subunits. (rndsystems.com)
  • Integrin alpha 6 beta 1 is a receptor for laminin. (rndsystems.com)
  • Integrin alpha 6 beta 4 is also a receptor for laminin and is a structural component of hemidesmosomes in stratified epithelia. (rndsystems.com)
  • Synthetic peptides from the carboxy-terminal globular domain of the A chain of laminin: their ability to promote cell adhesion and neurite outgrowth, and interact with heparin and the beta 1 integrin subunit. (rupress.org)
  • Antibodies against the beta 1 integrin subunit inhibited HT-1080 cell adhesion and neurite outgrowth on surfaces adsorbed with peptides GD-3 and GD-4. (rupress.org)
  • Furthermore, these results suggest that the sequences comprising peptides GD-3 and GD-4 use an integrin as a receptor, of which the beta 1 integrin subunit is a component for these various cell types. (rupress.org)
  • In short-term adhesion assays, the preferred substrate for HCE-T attachment is placental laminin. (nih.gov)
  • Twenty-four hours after plating on laminin substrate, TG1N and TG16 cells adopted a bipolar and elongated shape (Supplementary Fig. 1 B) and displayed high motility (mean velocities of 26.3 ± 0.6 µm/h and 25.7 ± 1.1 µm/h, respectively) without a predefined direction (Supplementary Fig. S1 C, Supplementary Movies S1 and S2 ), consistently with random motility pattern with high velocity previously reported for other GSC lines 30 . (nature.com)
  • Functional analyses indicated that some of the genes induced by dragonfly larvae caused an increase in laminins necessary for cell adhesion in the extracellular matrix. (biomedcentral.com)
  • residues 2,110-3,060) of the A chain of murine-derived laminin has been shown to promote heparin binding, cell adhesion, and neurite outgrowth. (rupress.org)
  • In addition, solutions of these peptides or antibodies generated against these peptides inhibited laminin-mediated HT-1080 cell adhesion. (rupress.org)
  • Therefore, laminin appears to have multiple, independent sequences in the G domain that serve a similar cell adhesion promoting function for different cell types. (rupress.org)
  • Laminins are found in an intricate lattice of proteins and other molecules that forms in the spaces between cells (the extracellular matrix). (medlineplus.gov)
  • The laminins attach (bind) to other proteins in the extracellular matrix and in the membrane of muscle cells, which helps maintain the stability of muscle fibers. (medlineplus.gov)
  • Some patients possess IgA and IgG antibodies toward laminin 332 in what Zone et al described as linear IgA/IgG bullous dermatosis. (medscape.com)
  • Polyclonal and monoclonal antibodies against chicken gizzard 5 '-nucleotidase inhibit the spreading process of chicken embryonic fibroblasts on laminin substratum. (morulaivf.com)
  • Laminin-332 polypeptides are targeted by auto-antibodies in human patients with mucous membrane (cicatricial) pemphigoid or, more rarely, subepidermal vesicular diseases that resemble epidermolysis bullosa acquisita (EBA) or bullous pemphigoid (BP). (morulaivf.com)
  • In all nine dogs, IgG auto-antibodies detected purified human laminin-332 by immunoblotting. (morulaivf.com)
  • Altered localization or expression levels of Ln-332, particularly its gamma2 subunit, are of prognostic value in a variety of cancers . (bvsalud.org)
  • Laminin is a heterotrimer made from α, β and γ chain subunits. (sigmaaldrich.com)
  • The N-terminal short arms consist of rod-like elements (domain III and V) formed by tandem arrays of laminin-type EGF modules and several globular domains: domains IV and domain VI (laminin N-terminal). (embl.de)
  • Laminin subunit alpha-1 is a protein that in humans is encoded by the LAMA1 gene. (wikipedia.org)
  • Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Laminin Alpha 1 (LAMa1) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. (signetlabs.com)
  • Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Mouse Laminin Alpha 1 (LAMa1) in samples from Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids with no significant corss-reactivity with analogues from other species. (signetlabs.com)
  • In RNA obtained from alveolar type II cells isolated from mice treated with Ad-Null or Ad-Cre 60 days earlier, all three products were significantly reduced confirming a knockdown of and and suggesting that no N-terminal laminin fragment is usually produced (Fig. 1D). (mundial-brasil2014.net)
  • Statistical analyses of human microarray data from the publicly available expression project in Oncology (expO) dataset , including examination of the distributions of Ln-332 subunit mRNA levels, identified a significant decrease in the Ln-332 beta3gamma2 mRNA ratio between normal (n = 10) and early-stage colon cancer (n = 29) specimens. (bvsalud.org)
  • This study was conducted to define the potential sequence(s) originating from the G domain of laminin with any of these functional activities. (rupress.org)
  • Deletion of this exon will result in loss of the two main splice isoforms encoded by the gene (the 3a and 3b laminin subunits) (Ryan et al. (mundial-brasil2014.net)
  • Immunological investigations consisted of direct immunofluorescence (IF), indirect IF with intact and salt-split canine gingival, and salt-split normal or laminin-332-deficient human skin, immunoblotting with purified human laminin-332 and immunoblotting with recombinant NC1 domain of human collagen VII. (morulaivf.com)
  • Description: A sandwich ELISA kit for detection of Laminin Alpha 1 from Mouse in samples from blood, serum, plasma, cell culture fluid and other biological fluids. (signetlabs.com)
  • The laminins can self-assemble, bind to other matrix macromolecules, and have unique and shared cell interactions mediated by integrins, dystroglycan, and other receptors. (embl.de)
  • GSL I has been reported to bind several glycoproteins including laminin. (vectorlabs.com)
  • In vitro assays demonstrated a competition of laminin and this monoclonal antibody for the binding site on purified 5′-nucleotidase. (morulaivf.com)
  • Characterization of high affinity binding between laminin and the acute-phase protein, serum amyloid A". J. Biol. (wikipedia.org)
  • ELAV - like1 , methyltransferase like 7A , dolichyl-phosphate mannosyltransferase , laminin subunit beta-1 , gremlin 1 , BCL6 corepressor-like 1 , and three genes of unknown identity. (biomedcentral.com)
  • The different laminin chains share a 600-residue domain I/II which oligomerises into a rod-like coiled-coil structure forming the long arm of laminins. (embl.de)
  • Mouse laminin is composed of three polypeptide chains (A: 400 kD, B1: 230 kD and B2: 220 kD), connected by disulphide bridges, and is glycosylated. (sigmaaldrich.com)
  • Most LAMA2 gene mutations that cause the severe, early-onset form of LAMA2 -related muscular dystrophy result in the absence of functional laminin alpha-2 subunit. (medlineplus.gov)
  • Mutations that cause the milder, later-onset form usually result in a reduction (deficiency) of functional laminin alpha-2 subunit. (medlineplus.gov)
  • Deficiency or absence of the laminin alpha-2 subunit results in a corresponding lack of laminin 2 and laminin 4, reducing the strength and stability of muscle tissue and leading to the signs and symptoms of LAMA2 -related muscular dystrophy. (medlineplus.gov)
  • There are two types of subunits, termed A and B, with slightly different molecular weights. (vectorlabs.com)
  • Laminin, alpha 1 has been shown to interact with FBLN2. (wikipedia.org)
  • The LAMA2 gene provides instructions for the alpha-2 subunit. (medlineplus.gov)
  • Association of variation in the LAMA3 gene, encoding the alpha-chain of laminin 5, with atopic dermatitis in a German case-control cohort. (cdc.gov)
  • The chimeric offspring were mated to C57BL/6 mice and ESC-derived progeny were sequentially bred to produce mice homozygous for the floxed 3 laminin subunit allele. (mundial-brasil2014.net)
  • Lung-specific knockdown of 3 laminin mice were treated with Ad-Cre or Ad-Null and 30 days, 60 days or 6 months later the lungs were harvested, homogenized and the abundance of the 3 laminin subunit was detected by immunoblotting. (mundial-brasil2014.net)
  • Laminin supports growth and differentiation of many cell types including epithelial, endothelial, neural, muscle and liver cells. (sigmaaldrich.com)
  • actin related protein 2/3 complex subunit 2. (gsea-msigdb.org)
  • Laminin is purified as laminin-nidogen complex from mouse Engelbreth-Holm-Swarm (EHS) sarcoma. (sigmaaldrich.com)
  • Spreading-arrested and rounded CEF do not develop prominent intracellular stress-fibers like control cells, instead they seem to concentrate their available actin in areas of presumptive initial contact with the laminin substratum. (morulaivf.com)
  • Western blot analysis was used to partially characterize commercially available preparations of laminin isolated from the mouse EHS sarcoma and from human placenta. (nih.gov)
  • These subunits combine to form tetrameric structures, resulting in five isolectins. (vectorlabs.com)
  • It is known as a 'LE' or 'laminin-type EGF-like' domain. (embl.de)
  • However, the subsequent spreading process of these cells was found to be inhibited for at least 2 h on a laminin substratum. (morulaivf.com)
  • Required for the assembly and/or stability of the 40S ribosomal subunit. (joplink.net)
  • Leukocyte adhesion deficiency type I (LAD I) is a failure to express CD18, which composes the common ß 2 subunit of LFA1 family (ß2 integrins). (medscape.com)