ProteinsFibronectinSignal transductionNeural cell adhMoleculesIntegrinInhibitionActin filamentLeukocytePlatelet activationProteinCytokinesMembraneHigh-affinityVascularLigandNeuronsPlateletsFibroblastsGPCROutgrowthTransmembraneProliferationExpressionCellEpidermalMetastasisRegulationInteractGrowthCalciumSurfacesCellsInterstitialOccursSymptomsVitroPrimarily
Proteins11
- The immunofluorescence following the increase of vinculin and talin, two cytoskeletal proteins, at the intracellular face of the plasma membrane with Val14rhoA microinjection showed the association of focal adhesions with the end of the new stress fibres. (wikipedia.org)
- We describe a novel synergistic effect between substrate-bound hemolymph proteins and laminin. (nature.com)
- Substrate-bound factor(s) from central nervous system-conditioned media also had a synergistic effect with laminin, suggesting a possible cooperation between humoral proteins and nervous system extracellular matrix. (nature.com)
- Extracellular matrix is a fibrillar network composed of structural (collagens) and adhesion (fibronectin) proteins crucial for tissue architecture and biological pathway Histol Histopathol 27:1515-1528, 2012 . (springer.com)
- This fibrillar network is composed of many structural proteins, such as collagens, and adhesion proteins, including fibronectin and proteoglycans (perlecan, decorin) [ 13 , 14 ]. (springer.com)
- A nanoscale modular design strategy was employed to synthesize six engineered, recombinant proteins intended to mimic aspects of the extracellular matrix proteins fibronectin, laminin, and elastin as well as the cell-cell adhesive protein neural cell adhesion molecule. (frontiersin.org)
- G protein-coupled receptors (GPCRs) constitute a large family of integral transmembrane receptor proteins that play a central role in signal transduction in eukaryotes. (biomedcentral.com)
- Several ECM proteins such as laminin, tenascin and thrombospondin contain domains with homology to epidermal growth factor (EGF) and exhibit growth promoting activity. (embl.de)
- Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. (cancer-genetics.org)
- Using mass spectrometry, quantitative proteomic analysis and Gene Ontology terms filters, we observed that EVs released by EGFRvIII-transformed cells were enriched for extracellular exosome and focal adhesion related proteins. (cancer-genetics.org)
- The presence of these proteins accounts for the transparency of the lens. (lookformedical.com)
Fibronectin3
- ECM components (i.e., fibronectin or collagens) directly interact with membrane receptors (i.e., integrins), particularly, by influencing cell behavior. (springer.com)
- This growth factor also stimulates production of collagens, proteoglycans, elastin, fibronectin, tenascin and thrombospondin, diminishes production of extracellularly active neutral endoproteinases belonging to the matrix metalloproteinase (MMP) and serine proteinase families, and stimulates production of endogenous MMP inhibitors (tissue inhibitor of metallo-proteinase [TIMP]) and serpins (plasminogen activator inhibitor-1). (biomedcentral.com)
- Previous studies have shown that small beads coated with FN7-10, a four-domain cell adhesion fragment of fibronectin, bind to cell surfaces and translocate rearward. (biologists.com)
Signal transduction3
- Important biological events associated with plasma membranes, such as signal transduction, cell adhesion, and protein trafficking, are mediated through the membrane microdomains. (mdpi.com)
- Cytokines bind to their receptors, activating signal transduction pathways such as adenylate cyclase/cAMP, phospholipase C/inositol trisphosphate, and Ca 2+ and tyrosine kinases. (biomedcentral.com)
- It first binds with high affinity to specific cell-surface receptors and then induces their dimerization, which is essential for activating the tyrosine kinase in the receptor cytoplasmic domain, initiating a signal transduction that results in DNA synthesis and cell proliferation. (embl.de)
Neural cell adh1
- In early culture, PSCs are polygonal in shape with abundant lipid droplets in the cytoplasm ( Figure 1B ) and express stellate cell selective markers such as desmin, glial fibrillary acidic protein (GFAP), nestin, neural cell adhesion molecule, nerve growth factor, and synemin (34, 167). (pancreapedia.org)
Molecules5
- Indeed, granule cells from the rostral region of perinatal Gpr56 −/− cerebella show loss of adhesion to extracellular matrix molecules of the pial basement membrane. (jneurosci.org)
- These studies establish a novel role for GPR56 in the adhesion of developing neurons to basal lamina molecules and suggest that this adhesion is critical for maintenance of the pia and proper cerebellar morphogenesis. (jneurosci.org)
- Consistent with this hypothesis, developing granule cells from the rostral region of Gpr56 −/− cerebella at birth exhibit a specific defect in cell adhesion to extracellular matrix (ECM) molecules of the pial basement membrane (BM) but no defects in migration, proliferation, or process outgrowth. (jneurosci.org)
- These factors include neurotrophins, such as Nerve Growth Factor (NGF), and adhesion molecules, such as laminin. (mun.ca)
- These findings support the notion that the newly uncovered properties of ALP to interfere with cytokine signalling and upregulation of adhesion molecules in endothelial cells are likely to contribute to the therapeutic potential of ALP in immune-complex-induced tissue injury. (biomedcentral.com)
Integrin10
- A key adhesion molecule expressed by these cells is the α v β 3 integrin. (rupress.org)
- Here, we show that although this receptor is present on most vascular and blood cells, the key regulatory function in tumor and wound angiogenesis is performed by β 3 integrin on bone marrow-derived cells (BMDCs) recruited to sites of neovascularization. (rupress.org)
- We demonstrate that α v β 3 integrin enhances BMDC recruitment and retention at angiogenic sites by mediating cellular adhesion and transmigration of BMDCs through the endothelial monolayer but not their release from the bone niche. (rupress.org)
- Adult Dorsal Root Ganglion (DRG) neurons plated on surfaces coated with a thin film of laminin exhibited increased neurite outgrowth, this growth was correlated with increased expression of laminin binding integrin subunits. (mun.ca)
- Laminin-induced neurite growth was integrin-dependent as it was attenuated by treatment with either a cyclic Arg-Gly-Asp (RGD) peptide or a β1 integrin-blocking antibody (β1i). (mun.ca)
- The addition of NGF to cells plated on laminin resulted in a significant and synergistic increase in the integrin-dependent outgrowth. (mun.ca)
- For lymphocytes and monocytes, the integrin α 2 β 1 is also capable of mediating primary adhesion under some flow conditions via its ligand vascular cell adhesion molecule (VCAM). (avsconferences.org)
- Secondary or firm adhesion is mediated by members of the integrin family on the cell surface, often after activation, in concert with their ligands on the solid. (avsconferences.org)
- While collagen serves as the main structural feature of the ECM and is an important ligand for integrin-based adhesions, HA acts as a ligand for the transmembrane glycoprotein CD44. (bvsalud.org)
- It is involved in cellular processes including cell adhesion and may regulate integrin trafficking and/or function. (cancer-genetics.org)
Inhibition2
- The inhibition of Rho by C3 transferase ribosylation resulted in an inhibition of focal adhesion and stress fibre assembly, but had no effect on membrane ruffling. (wikipedia.org)
- These inhibitory effects may be associated with its dual‑receptor inhibition on P2Y12 and TP receptors. (spandidos-publications.com)
Actin filament1
- The comparison with the normal cells showed that Rac1 stimulates actin filament production at the membrane, pinocytosis, and membrane ruffling. (wikipedia.org)
Leukocyte4
- Thus, intravital fluorescence microscopic imaging of leukocyte rolling and firm adhesion to postcapillary venules were performed in the knee joints of DBA1/J mice after intravenous injection of anti-CII mAbs. (biomedcentral.com)
- Leukocyte rolling and firm adhesion to the synovial endothelium in an early response to the anti-CII antibody transfer were significantly decreased in ALP-pretreated mice. (biomedcentral.com)
- A statistical analysis of intravital microscopic images recorded from the knee joints of untreated control mice during an early time span of 24 hours after the transfer of anti-CII mAbs [ 10 ] revealed an antibody-induced increase in leukocyte adhesion to the vessel walls. (biomedcentral.com)
- Preventive administration of ALP as a single dose of 100 μg per mouse led to a significant suppression of leukocyte rolling on, and of firm adhesion to, the synovial venular endothelium. (biomedcentral.com)
Platelet activation1
- Atherosclerosis starts with dysfunctional changes in the endothelium induced by disturbed shear stress which can lead to endothelial and platelet activation, adhesion of monocytes on the activated endothelium, and differentiation into proinflammatory macrophages, which increase the uptake of oxidized LDL (oxLDL) and turn into foam cells, exacerbating the inflammatory signalling. (hindawi.com)
Protein3
- In parallel with this experiment, Hall showed that the presence of Rac, another Ras-related GTP-binding protein, is implicated in the regulation of the actin organisation in presence of extracellular growth factors. (wikipedia.org)
- Indeed, Rho-dependent response is stimulated by the action of growth factors on Rac protein. (wikipedia.org)
- Mutations in GPR56 , an orphan G-protein-coupled receptor (GPCR), cause bilateral frontoparietal polymicrogyria (BFPP), a disorder characterized by mental retardation, seizures, motor developmental delay, and ataxia. (jneurosci.org)
Cytokines1
- Other profibrotic, collagen synthesis stimulating cytokines include endothelin, interleukin (IL)-1 and mast cell tryptase. (biomedcentral.com)
Membrane2
- Normal neuromuscular junction showing a presynaptic terminal with a motor nerve ending in an enlargement (bouton terminale): Synaptic cleft and postsynaptic membrane with multiple folds and embedded with several acetylcholine receptors. (medscape.com)
- Laminin is the most abundant glycoprotein present in basement membrane. (medicosplexus.com)
High-affinity2
- In this study, we demonstrate that the cross-linking of cell surface high-affinity IgE-R on mast cells derived from mouse bone marrow cultured for 3 wk in the presence of WEHI-3-conditioned media acts as a highly sensitive physiologic stimulus for this attachment and that receptor activation is also induced by calcium ionophore A23187. (aai.org)
- Hence, the high-affinity IgE-R on the mast cell functions not only in exocytosis but also facilitates the process of mast cell adherence to laminin. (aai.org)
Vascular1
- Upon vascular injury, platelets are exposed to the subendothelium, and several agonists, including adenosine diphosphate (ADP) and thrombin, are generated at the injury site, which can stimulate platelet adhesion, activation and aggregation. (spandidos-publications.com)
Ligand3
- Primary adhesion or tethering of the flowing cells requires very special properties of the cell surface receptor and its ligand on the solid structure because of the very short contact times available for bond formation and the forces on those bonds due to fluid drag. (avsconferences.org)
- Data from a radiolabeled ligand‑binding assay indicated that LGP exhibited apparent competing effects on thromboxane receptor (TP) and P2Y12 receptors. (spandidos-publications.com)
- Nuclear receptor coactivators associate in a ligand-dependent manner with estrogen receptors (ER) and other nuclear receptors, and they enhance ligand-dependent transcriptional activation. (bioone.org)
Neurons5
- Enhanced regeneration of dissociated bag cell neurons was observed in the presence of central nervous system (CNS) sheath cells or arterial cells 12 , or material remaining after such cells were killed 13 . (nature.com)
- In performing these experiments it was observed that not all DRG neurons were responding to laminin and NGF with increased neurite growth. (mun.ca)
- As neurons in the adult rat DRG can be classified into at least three separate subpopulations based on morphologic and phenotypic differences, a cell selection approach was utilized to show that laminin-induced neurite growth occurs in the absence of added trophic factors only in heavy chain neurofilament positive and calcitonin gene related peptide positive DRG neurons (NGF-responsive population). (mun.ca)
- In contrast, laminin alone is not sufficient to stimulate significant neurite growth from lectin Griffonia simplicifolia 1B4 positive neurons (IB4+ve), although it is still required to elicit a growth response from these cells in the presence of Glial Cell Line Derived Neurotrophic Factor (GDNF) (e.g., neurite growth occurred only when cells were plated on laminin in the presence of GDNF). (mun.ca)
- Further analysis indicated that Src was potentially a key point of collaboration between NGF and laminin induced neurite growth in NGF-responsive adult DRG neurons. (mun.ca)
Platelets1
- Platelets utilize a receptor complex GPIb-IX-V for this tethering process. (avsconferences.org)
Fibroblasts1
- Alan Hall showed the specificity of Rho in the stimulation of focal adhesions and stress fibres formation in fibroblasts in the presence of extracellular factors in 1992. (wikipedia.org)
GPCR2
- Phylogenetic analyses reveal that Ciona GPCRs have homologous representatives from the five major GRAFS ( Glutamate, Rhodopsin, Adhesion, Frizzled and Secretin ) families concomitant with other vertebrate GPCR repertoires. (biomedcentral.com)
- The Rhodopsin family accounts for ~68% of the Ciona GPCR repertoire wherein the LGR-like subfamily exhibits a lineage specific gene expansion of a group of receptors that possess a novel domain organisation hitherto unobserved in metazoan genomes. (biomedcentral.com)
Outgrowth1
- This combination increased outgrowth and branching relative to either laminin or hemolymph alone. (nature.com)
Transmembrane2
- A common structural feature of GPCRs is the presence of a highly conserved architecture of seven stretches of transmembrane spanning residues linked by alternate extracellular and intracellular loops. (biomedcentral.com)
- Integrins are transmembrane receptors that mediate cell adhesion by forming links between the extracellular matrix and cytoskeleton. (biologists.com)
Proliferation3
- One of the key processes involved in EC development is uncontrolled proliferation stimulated by local factors such as angiotensin. (spandidos-publications.com)
- Our study revealed that Ang II influences EC cells in terms of cancer-related processes, and is responsible for increased proliferation, reduction in apoptosis, increased mobility and modulation of adhesion potential. (spandidos-publications.com)
- One of the postulated key processes in the development of endometrial tumours is uncontrolled proliferation stimulated by physiological local factors ( 3 ). (spandidos-publications.com)
Expression1
- Hypoxia stimulates hypoxia inducible factor (HIF)-dependent expression of CXCL12 and KITL promoting mobilization from the bone marrow and recruitment to primary tumor and metastatic sites of CXCR4 + CD11b + bone marrow-derived cells and KITbCD11b + cells assisting vasculogenesis and metastasis respectively (Kuonen et al. (biomedcentral.com)
Cell4
- cancer cell growth and spreading, it has also been proposed that GPR56 regulates cell adhesion, but this has remained untested in the nervous system. (jneurosci.org)
- Methods for dissecting the specific molecular pathways involved for each step in the adhesion process for several cell types are given and the current state of our knowledge and potential applications are discussed. (avsconferences.org)
- Cell adhesion was increased on the hydrophobic CH 3 and the anionic COOH terminated surface. (avsconferences.org)
- The presence of the EG 3 and the PC moieties led to very low cell adhesion. (avsconferences.org)
Epidermal1
- Glioblastoma multiforme (GBM) is a highly aggressive and heterogeneous form of primary brain tumors, driven by a complex repertoire of oncogenic alterations, including the constitutively active epidermal growth factor receptor (EGFRvIII). (cancer-genetics.org)
Metastasis1
- Certainly, the presence of bone metastasis usually leads to worse outcome and shorter median survival [ 11 , 15 , 16 ]. (hindawi.com)
Interact1
- However, how these factors interact to stimulate optimal levels of neurite growth is largely unknown. (mun.ca)
Growth2
- Notably, the addition of hemolymph to laminin substrates accelerated growth cone migration rate over ten-fold. (nature.com)
- Src appeared to be located upstream of the PI3-K/Akt signalling pathway and was responsible for increased neurite growth induced by the addition of laminin and NGF together. (mun.ca)
Calcium1
- Adherence was inhibited by antibodies to laminin and laminin receptor and was calcium ion and temperature dependent. (aai.org)
Surfaces1
- Adhesion was studied on surfaces preadsorbed with fibrinogen or albumin, under well defined flow conditions using a radial flow chamber and automated videomicroscopy. (avsconferences.org)
Cells5
- We have reported that mast cells adhere to laminin after activation with PMA. (aai.org)
- however, culture of mast cells with TGF-beta 1 was determined to enhance IgE-mediated adherence to laminin. (aai.org)
- Interference RNA-mediated knockdown of GPR56 recapitulates the loss of adhesion seen in knock-outs, and reexpression of GPR56 rescues the adhesion defect in knock-out granule cells. (jneurosci.org)
- In Drosophila with depleted RACK1 in all muscle cells or, specifically, in SC lineage resulted in a delayed recovery of skeletal muscle after physical damage as well as the low presence of active SC in the wound area. (sdbonline.org)
- Sertoli cells have receptors for FSH. (medicosplexus.com)
Interstitial1
- The presence of RACK1 in interstitial SC was also detected. (sdbonline.org)
Occurs1
- The autoimmune attack occurs when autoantibodies form against the nicotinic acetylcholine postsynaptic receptors at the neuromuscular junction of skeletal muscles (see the image below). (medscape.com)
Symptoms1
- According to the American Society of Clinical Oncology (ANCO) guidelines on breast cancer follow-up and management, symptoms of breast cancer recurrence include presence of new breast lumps, pain in the bone, chest or abdomen, dyspnea and constant headaches ( 5 ). (iiarjournals.org)
Vitro1
- No change in the GM1/GD1a ratio after axotomy was observed in retinal axons ( in vitro and in vivo ), despite the presence of Neu3 sialidase. (jneurosci.org)
Primarily2
- The diversity among GPCRs primarily stem from the presence of characteristic N-terminal extracellular domains and C-terminal intracellular domains and to a relatively lesser extent from the connecting loops which share limited sequence similarity. (biomedcentral.com)
- Leukocytes utilize primarily members of the selectin family of receptors and their carbohydrate ligands for this first step - often resulting in a rolling interaction. (avsconferences.org)