Laminin gamma 2 antibody antibodies to laminin. Medical search. Frequent questions

Polyclonal and monoclonal antibodies against chicken gizzard 5 '-nucleotidase inhibit the spreading process of chicken embryonic fibroblasts on laminin substratum. (morulaivf.com)
Polyclonal and monoclonal antibodies raised against chicken gizzard 5′-nucleotidase were tested in adhesion assays of embryonic chicken fibroblasts (CEF) for their ability to interfere with the adhesion process of these cells on either laminin or fibronectin substrata. (morulaivf.com)
This effect was obtained with a polyclonal antibody as well as with one from 12 monoclonal antibodies raised against the native enzyme purified from chicken gizzard. (morulaivf.com)
In vitro assays demonstrated a competition of laminin and this monoclonal antibody for the binding site on purified 5′-nucleotidase. (morulaivf.com)
A hottest embodiment makes use of a novel human monoclonal antibody that binds to a conserved space of the gp41 transmembrane subunit of the virus. (kits-elisa.com)
Ramucirumab is an anti-VEGFR2 monoclonal antibody used for the therapy of a number of cancers. (ncbcs.org)
The following product was used in this experiment: CD3 Monoclonal Antibody (17A2), Super Bright™ 600, eBioscience™ from Thermo Fisher Scientific, catalog # 63-0032-82, RRID AB_2802423. (thermofisher.com)
C57BL/6 mouse splenocytes were stained with CD45R (B220) Monoclonal Antibody, FITC (Product # 11-0452-82) and 0.25 µg of Rat IgG2b kappa Isotype Control, Super Bright 600 (Product # 63-4031-82) (left) or 0.25 µg of CD3 Monoclonal Antibody, Super Bright 600 (right). (thermofisher.com)
Description: The 17A2 monoclonal antibody reacts with the mouse CD3 complex. (thermofisher.com)
AAN02 is a mouse monoclonal antibody against actin protein. (cytoskeleton.com)
We have used monoclonal antibodies (mAbs) as probes to study the dependence of the structure of different regions of the alphaM subunit on association with the beta2 subunit in the alphaM/beta2 heterodimer. (embl.de)

Immunological investigations consisted of direct immunofluorescence (IF), indirect IF with intact and salt-split canine gingival, and salt-split normal or laminin-332-deficient human skin, immunoblotting with purified human laminin-332 and immunoblotting with recombinant NC1 domain of human collagen VII. (morulaivf.com)
Desk 1 Antibodies useful for immunofluorescence. (cancercurehere.com)
Immunofluorescence images of mouse Swiss 3T3 cells stained with anti-actin antibody. (cytoskeleton.com)
Immunofluorescence staining using 1:500 dilution of anti-actin antibody in PBS is shown (green). (cytoskeleton.com)

CD3 subunits gamma, delta and epsilon are required for proper assembly, trafficking and surface expression of the TCR complex. (thermofisher.com)

Laminin-332 (laminin-5) is a basement membrane heterotrimeric protein composed of alpha-3, beta-3 and gamma-2 laminin chains. (morulaivf.com)
In five dogs, IgG variably recognized the basement membrane of laminin-332-deficient human skin (three dogs negative, two dogs positive). (morulaivf.com)
These observations establish laminin-332 as a novel basement membrane antigen in dogs with autoimmune blistering diseases with variable clinical phenotypes. (morulaivf.com)

The names 'acquired junctional epidermolysis bullosa', 'anti-laminin-332 mucous membrane pemphigoid (MMP)' and 'mixed auto-immune subepidermal blistering dermatosis' are proposed for dogs with clinical signs reminiscent of EBA, MMP or BP respectively. (morulaivf.com)
[ 4 ] Antilaminin 332 antibodies are typically seen in malignancy-associated mucous membrane pemphigoid (MMP). (medscape.com)

The initial attachment process of CEF on fibronectin and laminin substrata was not influenced by preincubating these cells with antibodies against chicken gizzard 5′-nucleotidase. (morulaivf.com)

The best-characterized antigen is a 97-kd protein extracted from human epidermis that binds IgA antibodies from sera of patients with linear IgA dermatosis. (medscape.com)

this antigen is recognized by circulating antibodies in some patients with linear IgA dermatosis, but it has not been further characterized. (medscape.com)

Furthermore, the expression of the genes known to govern insulin expression including Pdx1 and Ngn3 was markedly induced by laminin 411, which suggests that Pdx 1 and Ngn 3 signaling pathways are involved in laminin 411 induced-insulin expression machinery. (biomedcentral.com)
Our results demonstrate that laminin 411 acts as a potent differentiation inducer of IPCs from UC-MSCs via the Pdx1 and Ngn3 signaling pathways. (biomedcentral.com)
This Part 2 post is concerned with anti-cancer drug and other interventions that simultaneously address multiple growth pathways. (anti-agingfirewalls.com)

Immunoblot assays using human epidermal extracts and BP180 NC16a domain recombinant protein confirmed the presence of IgG antibodies against BP180 and recombinant BP180 NC16a domain protein. (morulaivf.com)

Spreading-arrested and rounded CEF do not develop prominent intracellular stress-fibers like control cells, instead they seem to concentrate their available actin in areas of presumptive initial contact with the laminin substratum. (morulaivf.com)
This antibody is recommended for detection of actin in a broad spectrum of species such as human, mouse, rat, and bovine. (cytoskeleton.com)
The immunogen used for antibody production was purified actin protein from rabbit skeletal muscle. (cytoskeleton.com)
The antibody has been shown to recognize α-skeletal, α-cardiac, α-smooth muscle, β-cytoplasmic, γ-smooth muscle and γ- cytoplasmic actin isotypes (Fig. 1) and has broad species cross-reactivity. (cytoskeleton.com)
Western blot of purified actin and cell extracts probed with anti-actin antibody. (cytoskeleton.com)
The blot was probed with a 1:1000 dilution of anti-actin antibody. (cytoskeleton.com)

Laminin-332 polypeptides are targeted by auto-antibodies in human patients with mucous membrane (cicatricial) pemphigoid or, more rarely, subepidermal vesicular diseases that resemble epidermolysis bullosa acquisita (EBA) or bullous pemphigoid (BP). (morulaivf.com)
Some patients possess IgA and IgG antibodies toward laminin 332 in what Zone et al described as linear IgA/IgG bullous dermatosis. (medscape.com)

mPEG-MAL (Thiol PEGylation of Proteins) - A Computationally Designed Inhibitor of an Epstein-Barr Viral Bcl-2 Protein Induces Apoptosis in Infected Cells, Cell, Volume 157, Issue 7, 19 June 2014, Pages 1644-1656, Text . (creativepegworks.com)
Netrins are a laminin-related family of matrix-binding secreted proteins containing an N-terminal laminin gamma-chain-related globular domain (domain VI), three laminin EGF-like repeats, and a C-terminal heparin-binding domain. (rndsystems.com)

Because antibodies that bind the 97- and 120-kd antigens do not recognize the 180-kd BPAg2, the former may express unique epitopes distinct from those of the parent protein. (medscape.com)
Laminin 411 markedly induced the expression of the genes Foxa2 and Sox17 , markers for pancreatic precursor cells, efficiently induced IPC differentiation from MSCs, and up-regulated insulin expression at both mRNA and protein levels. (biomedcentral.com)
Whereas, distinct presenilin 1 ( PSEN1 ) mutations lead to either (2) reduced γ-secretase activity, (3) altered protein stability or (4) reduced PSEN1 maturation, all culminating in reduced γ-secretase carboxypeptidase-like activity. (nature.com)
fAD-causing mutations in the genes coding for amyloid precursor protein ( APP ), presenilin 1 ( PSEN1 ) and presenilin 2 ( PSEN2 ) affect the production of Aβ in the central nervous system (CNS), implicating altered APP cleavage and processing in the AD disease mechanism [ 1 ]. (nature.com)
However, the complement C5a receptor 2 may act as a decoy receptor for C5a, as it has no reported G protein signalling capacity. (guidetomalariapharmacology.org)

In cases of linear IgA dermatosis induced by vancomycin, new lesions stop forming within approximately 2 weeks of withdrawal. (medscape.com)
Although an antibiotic, tetracycline has proven effective in linear IgA dermatosis, either alone or in conjunction with niacinamide (2 g/d). (medscape.com)

4,6-diamidino-2-phenylindole (DAPI) was useful for nuclei staining. (cancercurehere.com)

However, the subsequent spreading process of these cells was found to be inhibited for at least 2 h on a laminin substratum. (morulaivf.com)
The antibody is produced by regular cell traces developed using human B lymphocyte cells collected from a affected individual possessing extreme titers of anti-HIV antibodies. (kits-elisa.com)
Removal of floxed prevent sequences by Cre IKK-gamma (phospho-Ser376) antibody recombinase qualified prospects to long term labeling of transduced cells with YFP. (cancercurehere.com)
reporter mice had been injected with AAV8-CMV-Cre at postnatal day time 2 (P2) and cells had been analyzed at P10 and P56, respectively. (cancercurehere.com)
Open up in another window Shape 2 Quantification from the percentage of AAV8-CMV-Cre/YFP-labeled cells within CK19-expressing cholangiocytes and HNF4-expressing hepatocytes. (cancercurehere.com)
While most hepatocytes were labeled at this time point, 24.4% 7.5% of CK19+ cells were also labeled (Figure 1D and Figure 2). (cancercurehere.com)
Swiss 3T3 cells were grown to 25% confluency on poly-lysine and laminin coverslips. (cytoskeleton.com)
Cells were treated with a buffer control or 1 µg/ml of the Rho Activator (CN03) for 2 h at 37°C/5% CO 2 .to induce stress fibers. (cytoskeleton.com)

Antibody deposition leads to complement activation and neutrophil chemotaxis, which eventuates in loss of adhesion at the dermal-epidermal junction and in blister formation. (medscape.com)

Applications Reported: This 17A2 antibody has been reported for use in flow cytometric analysis. (thermofisher.com)
Applications Tested: This 17A2 antibody has been tested by flow cytometric analysis of mouse splenocytes. (thermofisher.com)

In all nine dogs, IgG auto-antibodies detected purified human laminin-332 by immunoblotting. (morulaivf.com)
These vaccines have been extensively studied, are currently in human trial phase 1/2, and seem to be the best treatment choice for pneumococcal diseases, especially for developing countries. (mdpi.com)
however, little is known about a role of laminin 411 in the regulation of IPC differentiation from human MSCs. (biomedcentral.com)

However, in numerous diseases it is well recognised that activation of the immune system causes self-damage 2 . (pocketdentistry.com)
In periodontitis, a breakdown of the periodontal tissues takes place during an immune response initially directed against oral microorganisms ( Fig 11-2 ). (pocketdentistry.com)

More importantly, administration of laminin 411-induced IPCs rapidly and significantly down-regulated fasting blood glucose levels, significantly reduced the HbA1c concentration and markedly improved the symptoms and survival of T1DM rats. (biomedcentral.com)

The present invention is extra directed to a package deal and a means for detecting the presence and determining focus of an antibody that inhibits HIV-1 fusion-associated epitope, a peptide on gp41 with the amino acid sequence represented by GCSGKLIC. (kits-elisa.com)
UC-MSCs were then cultured in the IPC induction and differentiation medium in the presence of laminin 411. (biomedcentral.com)

A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. (thermofisher.com)
J Cell Biol (1999) 145 (2): 305-315. (silverchair.com)
Consistent with this idea, there were significantly fewer free ends of microtubules in the cell bodies of neurons that had been injected with the katanin antibody compared with controls. (silverchair.com)

Prednisone stabilizes lysosomal membranes and suppresses lymphocyte and antibody production. (medscape.com)
bacterial or viral similarity with self-antigens (molecular mimicry) leading to production of cross-reactive antibodies. (pocketdentistry.com)

Edelman's Nobel Prize-winning research concerned discovery of the structure of antibody molecules. (academicinfluence.com)

They analyzed the role of MMP-2, MMP-8 and MMP-13 in radicular cysts, dentigerous cysts and keratocystic odontogenic tumors, and of MMP-1, MMP-7 and MMP-27 in keratocystic odontogenic tumors. (bvsalud.org)
MMP-1, MMP-2, MMP-3, MMP-8, MMP-9 and MMP-13 have been identified in odontogenic cysts, which suggests that MMPs play an important role in the growth of these tumors 9-10 . (bvsalud.org)

The transcription factors Oct4, Sox2, and Klf4 were found to trigger endogenous expression of pluripotent genes [2] and were identified as PTFs due to their ability to access closed chromatin [3] . (cytoskeleton.com)

Next, areas had been treated with primary antibodies (Desk 1) for over night at 4 C and supplementary antibodies for 2 h at space temp. (cancercurehere.com)
The primary antibody was detected with a 1:500 dilution of goat anti-mouse alexfluor-488 conjugated antibody. (cytoskeleton.com)

The conserved space positive by the neutralizing antibody is a peptide of roughly 12 amino acids in dimension and ideally 8-10 amino acids. (kits-elisa.com)

The N-terminal region has been predicted to fold into a beta-propeller domain composed of seven beta-sheets each about 60 amino acid residues long, with the I-domain inserted between beta-sheets 2 and 3. (embl.de)
Netrin-G1a and Netrin-G2a contain an N-terminal laminin globular domain (domain VI) followed by 3 laminin EGF-like repeats, and a C-domain with a hydrophobic signal for glycosylphosphatidylinositol (GPI) lipid linkage. (rndsystems.com)

In cases in which type VII collagen is the molecule against which the antibody response is directed, patients are less likely to be responsive to treatment. (medscape.com)

DM is briefly classified into two subtypes, type-1 (T1DM) and type-2 (T2DM). (biomedcentral.com)

Study of these mutations offers a powerful way of interrogating key underlying disease mechanisms for all forms of AD [ 2 ]. (nature.com)

When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions. (thermofisher.com)

Based on their thick layer of capsular polysaccharide (CPS), S. pneumoniae have been classified into about 97 different specific types according to the Danish classification system [ 2 , 3 ]. (mdpi.com)
mPEG-SH (Surface PEGylation of Gold Nanorods) - Conjugation of antibodies to gold nanorods through Fc portion: synthesis and molecular specific imaging, Bioconjug Chem. (creativepegworks.com)

Strategies: On this unmatched 1:1 case-control examine, blood samples from 46 real-time RT-PCR-confirmed SARS-CoV-2 hospitalized circumstances and 45 wholesome donors (adverse controls) have been studied. (kits-elisa.com)

HNF4 and CK19 expressions had been special mutually, and nearly all hepatocytes were called expected, as the control vector AAV8-CMV-RFP didn't result in YFP manifestation (Shape 1B,C). Remarkably, we detected Cre/YFP-marked cholangiocytes at P10 with 11 also.6% 7.8% (mean SD) labeling efficiency (Figure 1C and Figure 2). (cancercurehere.com)
Interestingly, there was a statistically significant increase in the percentage of labeled cholangiocytes from P10 to P56 (Figure 2). (cancercurehere.com)
Figure 2. (cytoskeleton.com)

Microinjection of an antibody that inactivates katanin results in a dramatic accumulation of microtubules at the centrosome, indicating that katanin is indeed required for microtubule release from the centrosome. (silverchair.com)

for the first time, reported that sclerosing pancreatitis is related to high levels of immunoglobulin G4 (IgG4) [ 2 ]. (hindawi.com)

Anatomy18

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Diseases3

Chemicals and Drugs79

Analytical, Diagnostic and Therapeutic Techniques and Equipment34

Phenomena and Processes35

Disciplines and Occupations2

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Monoclonal11

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Subunits1

Basement membrane3

Mucous membrane p2

Fibronectin1

Binds1

Antigen1

Pathways3

Assays1

Actin6

Bullous2

Proteins2

Protein5

Dermatosis2

DAPI1

Cells8

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Human3

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Cell3

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