Lactococcus lactis. Medical search. Frequent questions

L. lactis subsp. (wikipedia.org)
Lactococcus lactis subsp. (online-medical-dictionary.org)
We report the first description, confirmed by bacteriologic and molecular (polymerase chain reaction and pulsed-field gel electrophoresis) analysis, of an infection in animals caused by Lactococcus lactis subsp. (cdc.gov)
Regulon of UxuR in Lactococcus lactis subsp. (lbl.gov)
Lactococcus lactis has two subspecies with few phenotype and genotype differences, Lactococcus lactis subsp. (locke-movie.com)
lactis and subsp. (locke-movie.com)
lactis is preferred for making soft cheese while subsp. (locke-movie.com)
AbiV, a novel antiphage abortive infection mechanism on the chromosome of Lactococcus lactis subsp. (omicsdi.org)
ISS1 resulted in independent insertions in a 350-bp region of the chromosome of Lactococcus lactis subsp. (omicsdi.org)
Στην παρούσα μελέτη, το στέλεχος L. lactis subsp. (aua.gr)
In our study, L. lactis subsp. (aua.gr)

lactis and cremoris), acidified faster than its unpressurised equivalent. (researchgate.net)
cremoris MG1363 was subjected to various stressful conditions in order to accomplish the phenotypic characterization of the adapted L. lactis cells. (aua.gr)

in the genome of L. lactis MG1363. (wikipedia.org)
Conjugal transfer of Tn6098 was demonstrated from the plant-derived donor strain L. lactis KF147 to the recipient L. lactis NZ4501, a derivative of the dairy model strain L. lactis MG1363. (ru.nl)
One copy of this sequence is also present in the L. lactis MG1363 chromosome and represents the sole integration site. (ru.nl)
Phenotypic characterization of all strains showed that the transconjugant has not only acquired the ability to grow well in soy milk, a substrate rich in alpha-galactosides, but also has retained the flavor-forming capabilities of the recipient strain L. lactis MG1363. (ru.nl)
Reverse transcription-PCR analysis confirmed that there were higher levels of transcription of a downstream open reading frame (ORF) in the phage-resistant integrants than in the phage-sensitive strain L. lactis MG1363. (omicsdi.org)
This gene was also found to confer phage resistance to L. lactis MG1363 when it was cloned into an expression vector. (omicsdi.org)
Low-temperature adaptation and cryoprotection were studied in the lactic acid bacterium Lactococcus lactis MG1363. (rug.nl)
Using two-dimensional gel electrophoresis a group of 7 kDa cold-induced proteins (CSPs) was identified that corresponds to a previously described family of csp genes of L. lactis MG1363. (rug.nl)
Fermentation-induced variation in heat and oxidative stress phenotypes of Lactococcus lactis MG1363 reveals transcriptome signatures for robustness. (uva.nl)

L. lactis is one of the best characterized low GC Gram positive bacteria with detailed knowledge on genetics, metabolism and biodiversity. (wikipedia.org)
Bacteriophages specific to L. lactis cause significant economic losses each year by preventing the bacteria from fully metabolizing the milk substrate. (wikipedia.org)
Lactose fermentation In one study that sought to prove that some fermentation produced by L. lactis can hinder motility in pathogenic bacteria, the motilities of Pseudomonas, Vibrio, and Leptospira strains were severely disrupted by lactose utilization on the part of L. lactis. (wikipedia.org)
Nisin is a natural antimicrobial peptide used as food preservative produced by Lactococcus lactis , that inhibits the outgrowth of spores, the growth of a variety of Gram-positive and Gram-negative bacteria. (usp.br)
An oral rinse solution containing a strain of the bacteria Lactococcus lactis (L. lactis) genetically modified to express human Trefoil Factor 1 (hTFF1) with potential cytoprotective activity. (medicalterminologydb.com)
The results showed that adding B. subtilis and L. lactis , particularly in MIX 300 , reduced the anaerobic heterotrophic bacterial microbiota and increased lactic acid bacteria (LAB) in fish. (ij-aquaticbiology.com)
On top of a microscope is a climate chamber, with inside a glass slide with a tiny growth chamber for the Lactococcus lactis bacteria. (rug.nl)
The lactic acid bacteria counts of coalho cheese from producers A and B were 106 and the highest counts (109 UFC/g) were found in cheese samples from producer C. Forty-nine lactic acid bacteria from three rural properties were selected and predominant genera was Enterococcus, Lactococcus, Streptococcus and Leuconostoc. (bvsalud.org)
Of 20 bacteria isolated, 19 showed inhibition halos on the three pathogenic bacteria with diameter of 2 to 15 mm, and the largest halos were formed by Lactococcus lactis ssp lactis on S. enterica and S. aureus. (bvsalud.org)

Lactococcus lactis is a gram-positive bacterium used extensively in the production of buttermilk and cheese, but has also become famous as the first genetically modified organism to be used alive for the treatment of human disease. (wikipedia.org)
lactis is added to milk, the bacterium uses enzymes to produce energy molecules (ATP), from lactose. (wikipedia.org)
Among the bacterial systems available, the Gram-positive lactic bacterium, Lactococcus lactis , traditionally used in food fermentations, is nowadays widely used for large-scale production and functional characterization of bacterial and eukaryotic membrane proteins. (springer.com)
Total LLO yield (measured as total protein content) was 4.43-5.9 mg per litre culture and the haemolytic activity was still detectable after 8 months of storage at 4°C. Conclusion: The LLO production method described in this work provides an approach to efficient LLO production in the Gram-positive Lactococcus bacterium to yield a significant source of the protein for research and diagnostic applications. (ucc.ie)
These observations may indicate similar self-protection mechanisms of the bacterium under different stressful conditions that need to be further investigated in order to shed new light on the rational use of L. lactis in the food industry. (aua.gr)
IMMUSE Lactococcus lactis strain Plasma (LC Plasma), is a unique strain of lactic acid bacterium, a postbiotic discovered and patented by parent company, Kirin. (nutraingredients-usa.com)

The autolysis and proteolysis are important features in the strains of L. lactis used in the manufacture of cheese. (scirp.org)
The objective of this work was to assess the autolytic and proteolytic activities of 21 strains of L. lactis iso lated from diverse sources. (scirp.org)
C. Nájera-Domínguez and N. Gutiérrez-Méndez, "Autolytic and Proteolytic Properties of Strains of Lactococcus lactis Isolated from Different Vegetables, Raw-Milk Cheeses and Commercial Starter Cultures," Food and Nutrition Sciences , Vol. 4 No. 11A, 2013, pp. 21-26. (scirp.org)
J. Ramírez-Nunez, R. Romero-Medrano, G. V. NevárezMoorillon and N. Gutiérrez-Méndez, "Effect of pH and Salt Gradient on the Autolysis of Lactococcus lactis Strains," Brazilian Journal of Microbiology, Vol. 42, No. 4, 2011, pp. 1495-1499. (scirp.org)
Special strains of L.lactis support the immune system specifically. (avocadoninja.co.uk)
Plasmacytoid dendritic cells (pDCs) are a rare immune cell type found exclusively in L.lactis strains. (avocadoninja.co.uk)
Most dairy lactococcus strains are unable to use alpha-galactosides as a growth substrate, yet many of these strains are known to have beneficial industrial traits. (ru.nl)
Diversity in robustness of Lactococcus lactis strains during heat stress, oxidative stress, and spray drying stress. (uva.nl)

Kirin Holdings Co, Ltd. developed Lactococcus lactis strain Plasma (heat-killed) as a clinically researched probiotic. (avocadoninja.co.uk)
The heated strain of Lactococcus lactis is both paraprobiotic and immunobiotic. (avocadoninja.co.uk)
Human studies have demonstrated that Lactococcus lactis strain Plasma (heat-killed) supports healthy working days and immunity during periods of high-intensity exercise. (avocadoninja.co.uk)
The integration of Tn6098 into the genome of the recipient strain was confirmed by Illumina sequencing of the transconjugant L. lactis NIZO3921. (ru.nl)
The cryptic plasmids from this strain were coelectroporated, along with the shuttle vector pSA3, into the plasmid-free host L. lactis LM0230. (omicsdi.org)
Lacticin Z showed homology to lacticin Q from L. lactis QU 5, aureocin A53 from Staphylococcus aureus A53, and mutacin BHT-B from Streptococcus rattus strain BHT. (elsevierpure.com)
Strain-Dependent Transcriptome Signatures for Robustness in Lactococcus lactis . (uva.nl)

Lactococcus) indicates the genus. (avocadoninja.co.uk)

LeBlanc DJ, Crow VL, Lee LN, Garon CF. Influence of the lactose plasmid on the metabolism of galactose by Streptococcus lactis. (harvard.edu)
A 34-amino acid polypeptide antibiotic produced by Streptococcus lactis. (lookformedical.com)
Streptococcus lactis diacetilactis (ex Matuszewski et al. (locke-movie.com)

A designed omp31 gene fused to the usp45 signal peptide and M6 cell wall anchor was sub cloned in the pNZ7021 expression vector, and a recombinant L. lactis displaying Omp31 was constructed. (ac.ir)
sIgA, serum IgA, IgM, and total IgG antibodies significantly increased in the mice immunized with live recombinant L. lactis expressing Omp31 and also serum IgM, and total IgG antibodies significantly increased in mice immunized with killed recombinant L. lactis expressing Omp31. (ac.ir)

Bonds between fibronectin and fibronectin-binding proteins on Staphylococcus aureus and Lactococcus lactis. (duke.edu)
queijo, Lactococcus, Staphylococcus aureus. (bvsalud.org)

lactis biovar diacetyllactis. (ichacha.net)

The aim of this chapter is to describe the different possibilities for the functional characterization of peripheral or intrinsic membrane proteins expressed in Lactococcus lactis . (springer.com)
Molecular characterization of a new abortive infection system (AbiU) from Lactococcus lactis LL51-1. (omicsdi.org)
This study reports on the identification and characterization of a novel abortive infection system, AbiU, from Lactococcus lactis. (omicsdi.org)

A non-pathogenic species of LACTOCOCCUS found in DAIRY PRODUCTS and responsible for the souring of MILK and the production of LACTIC ACID . (online-medical-dictionary.org)
The second part (e.g. lactis) indicates the species. (avocadoninja.co.uk)

Many different expression systems of L. lactis have been developed and used for heterologous protein expression. (wikipedia.org)
Pontes DS, de Azevedo MS, Chatel JM, Langella P, Azevedo V, Miyoshi A (2011) Lactococcus lactis as a live vector: heterologous protein production and DNA delivery systems. (springer.com)
Lactococcus lactisMG1363 expresses a protein, YfiA(Ll), which associates with ribosomes in the stationary phase of growth and is responsible for dimerization of ribosomes. (rug.nl)

Para tanto as células de L. lactis foram desenvolvidas em agitador rotacional (30°C/36 h/100 rpm) e a atividade de nisina, os parâmetros de crescimento e os componentes do meio de cultivo foram analisados. (usp.br)
As contagens de bactérias láticas do queijo de coalho dos produtores A e B foram na ordem de 106 e as maiores contagens (109 UFC/g) foram observadas no queijo do produtor C. Foram selecionadas 49 bactérias láticas típicas das três propriedades e os gêneros predominantes foram: Enterococcus, Lactococcus, Streptococcus e Leuconostoc. (bvsalud.org)
Dos 20 isolados, 19 apresentaram halos de inibição sobre as três bactérias patogênicas, com zonas de inibição de 2 a15 mm, e os maiores halos foram formados por Lactococcus lactis ssp lactis sobre S. enterica e S. aureus. (bvsalud.org)

Lactococcus lactis W-37 is highly resistant to phage infection. (omicsdi.org)
In addition to pSA3, erythromycin- and phage-resistant isolates carried pSRQ900, an 11-kb plasmid from L. lactis W-37. (omicsdi.org)
Samples were then analyzed for the presence of two Lactococcus lactis phage groups (936 and c2) and quantification was done by qPCR. (cdc.gov)

Lactose fermentation by L. lactis produces acetate that reduces the intracellular pH of Salmonella, which in turn slows the rotation of their flagella. (wikipedia.org)

Consistent with the results from comparative genomics (see references above), this resulted in L. lactis losing or downregulating genes that are dispensable in milk and the upregulation of peptide transport. (wikipedia.org)
These experiments were repeated using Lactococcus lactis constructs expressing fnbA and fnbB genes from S. aureus. (duke.edu)

Lactococcus lactis has been demonstrated to be a promising candidate for the delivery of functional proteins because of its noninvasive and nonpathogenic characteristics. (wikipedia.org)
Kunji ERS, Slotboom DJ, Poolman B (2003) Lactococcus lactis as host for overproduction of functional membrane proteins. (springer.com)
Monné M, Chan KW, Slotboom DJ, Kunji ERS (2005) Functional expression of eukaryotic membrane proteins in Lactococcus lactis. (springer.com)

Lactococcus lactis is of crucial importance for manufacturing dairy products, such as buttermilk and cheeses. (wikipedia.org)

A 15-bp direct repeat sequence (TTATACCATAATTAC) is present on either side of Tn6098 in the chromosome of L. lactis KF147. (ru.nl)

lactis infection in waterfowl: first confirmation in animals. (cdc.gov)
AbiQ, an abortive infection mechanism from Lactococcus lactis. (omicsdi.org)

2019. Pengaruh Pemberian Probiotik Lactococcus lactis dan Bifidobacterium terhadap Produksi Ayam Petelur yang Diinfeksi Escherichia coli. (unila.ac.id)

It is suggested that the L. lactis supernatant mainly affects Salmonella motility through disruption of flagellar rotation rather than through irreversible damage to morphology and physiology. (wikipedia.org)
Lactococcus lactis In terms of cell morphology, lactococci have spherical or ovoid-shaped cells and occur singly or in chains (Figure 1). (locke-movie.com)

Kinetic studies on α-acetolactic acid extra and intracellular oxidative décarboxylation to diacetyl by Lactococcus lactis ssp. (dairy-journal.org)

Based on its history in food fermentation, L. lactis has generally recognized as safe (GRAS) status, with few case reports of it being an opportunistic pathogen. (wikipedia.org)
The use of SFSp as fermentation medium for l-lactic acid production generated maximum biomass concentration of 14.53 g/L. The utilisation of SFSp extracted from pre-harvest sago frond as substrate promote efficiency of l-lactic acid production associated to exceptional growth performance of L. lactis IO-1. (unimas.my)

This study aims to evaluate growth conditions for L. lactis as well as the effect in nisin production when utilizing milk whey and skimmed milk. (usp.br)
Lactococcus lactis ATCC 11454 was developed in a rotatory shaker (30°C/36 h/100 rpm) in diluted skimmed milk and nisin expression, growth parameters and media components were also studied. (usp.br)
Milk whey, abyproduct from dairy industries, was utilized in two different ways (i) without filtration, autoclaved at 121°C for 30 min and (ii) filtrated (1.20 µm and 0.22 µm membrane filter), L. lactis was developed in a rotary shaker (30°C/36 h/100 rpm) and these cultures were transferred five times using 5 mL aliquots of broth culture for each new volume of the respective media. (usp.br)
The results showed that culture media composed by milk whey without filtration was better for L. lactis in its adaptation than milk whey without filtration. (usp.br)

It is concluded that the dimerization and inactivation of ribosomes in L.lactis and E.coli differ in several cellular and molecular aspects. (rug.nl)
These results indicated that recombinants L. lactis induce both humoral and cellular immune responses in mice, and also vaccines based on L. lactis-derived live carriers are promising interventions against Brucella melitensis infections. (ac.ir)
Fourier transformed infrared spectroscopy (FT-IR) was applied in order to evaluate the changes in the cellular composition of L. lactis adapted cells. (aua.gr)

L. lactis does not produce spores (nonsporulating) and are not motile (nonmotile). (wikipedia.org)

Here we show that a combination therapy of low-dose anti-CD3 with a clinical-grade self-containing L. lactis , appropriate for human application, secreting human proinsulin and interleukin-10, cured 66% of mice with new-onset diabetes, which is comparable to therapy results with plasmid-driven L. lactis . (diabetesgeneeskunde.nl)

Results: The constructed vector (pNZPnisA:CYTO-LLO) was expressed in L. lactis NZ9000 and was best induced at mid-log phase with 0.2% v/v nisin for 4 h statically at 30°C. Purification of the His-tagged LLO was accomplished by Ni-NTA affinity chromatography and functionality was confirmed through haemolytic assays. (ucc.ie)
Accordingly, Lactococcus lactis has been used as an antigen-delivering vector to develop a vaccine-induced mucosal response for having a safer vaccination against brucellosis. (ac.ir)

Here, we present the high-resolution crystal structures of the wild-type and the P1G defective mutant of Fpg from Lactococcus lactis bound to 14mer DNA duplexes containing either a tetrahydrofuran (THF) or 1,3-propanediol (Pr) AP site analogues. (rcsb.org)

Heat-killed Lactococcus lactis JCM 5805 is sometimes called LC-Plasma and other times LC-Plasma in clinical studies. (avocadoninja.co.uk)

By contrast, ectopic expression of FnBPA or FnBPB on the surface of L. lactis conferred fibronectin binding characteristics similar to those of S. aureus. (duke.edu)

E. H. E. Ayad, A. Verheul, J. T. M. Wouters and G. Smit, "Antimicrobial-Producing Wild Lactococci Isolated from Artisanal and Non-Dairy Origins," International Dairy Journal, Vol. 12, No. 2-3, 2002, pp. 145-150. (scirp.org)

The use of L. lactis in dairy factories is not without issues. (wikipedia.org)

We show that full-length YfiA(Ll) is necessary and sufficient for ribosome dimerization in L.lactis but also functions heterologously in vitro with E.coli ribosomes. (rug.nl)
In addition, two-dimensional maps of dimeric ribosomes from L.lactis obtained by single particle electron microscopy show a marked structural difference in monomer association in comparison to the ribosome dimers in E.coli. (rug.nl)
Expression of LLO in L. lactis has a number of benefits over E. coli which may facilitate both in vivo and in vitro applications of this system. (ucc.ie)

L. lactis is mainly isolated from either the dairy environment, or plant material. (wikipedia.org)

This work aimed to investigate the effect of dietary Lactococcus lactis and Bacillus subtilis on the immune responses, intestinal microbiota, and resistance to pathogens of Oscar, Astronotus ocellatus . (ij-aquaticbiology.com)
The assessment of the immune changes induced by the L. lactis -based therapy revealed elevated frequencies of CD4 + Foxp3 + T cells in the pancreas-draining lymph nodes, pancreas, and peripheral blood of all treated mice, independent of metabolic outcome. (diabetesgeneeskunde.nl)

Transcriptome Analysis of a Spray Drying-Resistant Subpopulation Reveals a Zinc-Dependent Mechanism for Robustness in L. lactis SK11. (uva.nl)

The introduction of β-cell autoantigens via the gut through Lactococcus lactis ( L. lactis ) has been demonstrated to be a promising approach for diabetes reversal in NOD mice. (diabetesgeneeskunde.nl)

This study aims to determine the use of probiotics Lactococcus lactis and Lactobacillus casei. (unila.ac.id)

Deletion of the yfiA gene has no effect on the growth rate but diminishes the survival of L.lactis under energy-starving conditions. (rug.nl)
This revealed that L. lactis cells overproducing CspD at 30 °C show a 2-10-fold increased survival after freezing compared to control cells. (rug.nl)

de Ruyter PG, Kuipers OP, de Vos WM (1996) Controlled gene expression systems for Lactococcus lactis with the food-grade inducer nisin. (springer.com)

L. lactis cells are cocci that group in pairs and short chains, and, depending on growth conditions, appear ovoid with a typical length of 0.5 - 1.5 µm. (wikipedia.org)
Environmental stresses comprised of acid (pH 5.5), osmotic (presence of 2% w/v NaCl), heat (45oC), and cold (20oC) stress, as well as responses of L. lactis cells during starvation (media without carbohydrate source). (aua.gr)
Principal component analysis of the second derivative transformed spectra revealed different groups of L. lactis cells corresponding to each stressful condition applied in the study for all characteristic spectral regions. (aua.gr)

Where is Lactococcus lactis found in the wild? (locke-movie.com)
Aside from its high use in industrial application, Lactococcus lactis can also be found in the wild on plants and within the digestive tract of cows. (locke-movie.com)

Mierau I, Kleerebezem M (2005) 10 years of the nisin-controlled gene expression system (NICE) in Lactococcus lactis. (springer.com)
In the present work, Lactococcus lactis NZ9000 was used as an expression host to hyper-produce LLO under inducible conditions using the NICE (NIsin Controlled Expression) system. (ucc.ie)

Use of non-growing Lactococcus lactis cell suspensions for production of volatile metabolites with direct relevance for flavour formation during dairy fermentations. (uva.nl)

The pH and the NaCl concentration in the media affected significantly the autolysis of L. lactis . (scirp.org)

Lactococcus lactis - Taxon details on National Center for Biotechnology Information (NCBI). (wikimedia.org)

N. Gutiérrez-Méndez, B. Vallejo-Cordoba, A. F. González-Córdova, G. V. Nevárez-Moorillon and B. RiveraChavira, "Evaluation of Aroma Generation of Lactococcus lactis with an Electronic Nose and Sensory Analysis," Journal of Dairy Science, Vol. 91, No. 1, 2008, pp. 49-57. (scirp.org)

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