Chemicals and DrugsAnalytical, Diagnostic and Therapeutic Techniques and EquipmentInformation Science
beta-LactamasesCephaloridineMicrobial Sensitivity TestsAnti-Bacterial AgentsEncyclopedias as TopicPenicillinaseLactamsCyclacillinCarbapenems
ESBLInhibitorsExtended-spectrumEscherichiaPseudomonasCephalosporinsIsolatesSerineENTEROBACTERIACEAEEnzymesBacteriaBeta-lactamase geneCharacterizationResistanceHydrolysisStrainsEnzymeAmpCMetallo betaHydrolyzePenicillinsDetectionCarbapenemaseHaemophilusGeneChromogenicCarbapenemasesProteinsGenesProteinPlasmidInhibitionMechanismsInhibitorGeneticClass
ESBL9
  • The infections caused by the Extended Spectrum Beta Lactamase (ESBL) enzymes-producing E. coli hinder antibiotic treatment. (nih.gov)
  • In addition, hemolysis and production of Extended Spectrum Beta-Lactamases (ESBL) was assessed in the isolates. (scielo.br)
  • As adequate and rapid screening methods for antibiotic resistance are crucially needed, the present study investigated the discrimination potential of MALDI-TOF MS among extended-spectrum-beta-lactamase (ESBL) or metallo-beta-lactamases- (MBL) producing and the nonproducing strains of Escherichia coli (n=19), Klebsiella pneumoniae (n=19), and Pseudomonas aeruginosa (n=38), respectively. (medscimonit.com)
  • The extended-spectrum β-lactamase (ESBL)-producing bacteria have been isolated at increasing frequency worldwide. (scielo.br)
  • O isolamento de bactérias produtoras de beta-lactamases de espectro expandido (ESBL) está aumentando no mundo todo. (scielo.br)
  • Isolates were screened for antibiotic resistance, to include extended spectrum beta-lactamase (ESBL) production, via the Microscan Automated Microbiology System. (biomedcentral.com)
  • First of all, it concerns pathogens producing expanded spectrum β-lactamases, ESBL. (assignology.com)
  • The aim of this study was to assess the impact of multiple β-lactamase carriage on the accuracy of susceptibility tests and extended-spectrum β-lactamase (ESBL) and carbapenemase confirmation methods. (udayton.edu)
  • Extended-spectrum ß-lactamase (ESBL)-producing Escherichia coli has been increasingly recognized as the cause of upper urinary tract infection (UTI) in children . (bvsalud.org)
Inhibitors15
  • Chromosomal-mediated AmpC β-lactamases represent a new threat, since they confer resistance to 7-alpha-methoxy-cephalosporins (cephamycins) such as cefoxitin or cefotetan but are not affected by commercially available β-lactamase inhibitors, and can, in strains with loss of outer membrane porins, provide resistance to carbapenems. (wikipedia.org)
  • Metallo-β-lactamases, long-considered as of little clinical relevance, now present a serious global threat to almost all BLAs, rendering the development of approaches to MBL inhibitors important. (europa.eu)
  • Unlike the serine β-lactamases, the MBLs are structurally and mechanistically unrelated to PBPs, and are not inhibited by current mechanism-based SBL inhibitors. (europa.eu)
  • Beta-lactam and Beta-lactamase Inhibitors Market Like to. (pharmiweb.com)
  • Pune, Maharashtra, India, February 22 2021 (Wiredrelease) Allied Analytics -:Beta-lactam and beta-lactamase inhibitors are chemical compounds of natural or semi-synthetic or synthetic origin. (pharmiweb.com)
  • The beta-lactam and beta-lactamase inhibitors market is expected to exhibit significant growth during the forecast period due to increased consumption in low and middle-income countries (LMICs) and rise in prevalence of infectious diseases. (pharmiweb.com)
  • In addition, development of novel approaches for new beta-lactam and beta-lactamase inhibitors for treating bacterial infections and a large number of clinical trials, further supplement the market growth. (pharmiweb.com)
  • The cephalosporin segment accounted more than two-fifths of the total beta-lactam and beta-lactamase inhibitors market share in 2018. (pharmiweb.com)
  • However, development of antibiotic resistance, driven by misuse of beta-lactam and beta-lactamase inhibitors and the time taken for the regulatory approval is projected to impede the market growth. (pharmiweb.com)
  • Combination segment is further categorized into penicillin/beta lactamase inhibitors, cephalosporins/beta lactamase inhibitors, and carbapenems/beta lactamase inhibitors. (pharmiweb.com)
  • Asia-Pacific was the leading revenue contributor to the global beta-lactam and beta-lactamase inhibitors market in 2018, and is expected to remain dominant throughout the forecast period. (pharmiweb.com)
  • The report provides a comprehensive analysis of the key players operating in the global beta-lactam and beta-lactamase inhibitors market industry, namely, Abbott Laboratories, Allergan Plc. (pharmiweb.com)
  • U.S. generated the highest revenue in the global beta-lactam and beta-lactamase inhibitors in 2018, accounting more than one-fifth of the global market in 2018. (pharmiweb.com)
  • Tony Fink's work contributed greatly to our understanding of the mechanisms and active site function of beta-lactamases and this knowledge now informs the search for new beta-lactams and beta-lactamase inhibitors. (uea.ac.uk)
  • Efforts to develop metallo-β-lactamase (MBL) inhibitors require suitable screening platforms to allow the rapid determination of β-lactamase activity and efficient inhibition. (ox.ac.uk)
Extended-spectrum8
  • P. mirabilis) due to the production of TEM- or SHV-type ESBLs (extended spectrum beta lactamases). (wikipedia.org)
  • In the mid-1980s, this new group of enzymes, the extended-spectrum β-lactamases (ESBLs), was detected (first detected in 1979). (wikipedia.org)
  • ESBLs are beta-lactamases that hydrolyze extended-spectrum cephalosporins with an oxyimino side chain. (wikipedia.org)
  • The mechanisms include extended-spectrum β-lactamases (ESBLs), AmpC β-lactamases, and carbapenemases of molecular classes A and B. (asm.org)
  • In Enterobacterales , the prime antimicrobial resistance mechanism against β-lactam antibiotics is mainly the production of β-lactamases, particularly extended-spectrum β-lactamases (ESBLs). (frontiersin.org)
  • Thus, penicillin G was undermined by swift accumulation of staphylococcal penicillinase, ampicillin by TEM-1 enzyme and modern oxymino cephalosporins by "extended-spectrum" beta-lactamases. (uea.ac.uk)
  • Does the presence of multiple β-lactamases in Gram-negative bacilli impact the results of antimicrobial susceptibility tests and extended-spectrum β-lactamase and carbapenemase confirmation methods? (udayton.edu)
  • Cases of Pediatric Pyelonephritis: A Single-Center Retrospective Study from an Extended-Spectrum ß-Lactamase-Producing Escherichia coli Endemic Area in Japan. (bvsalud.org)
Escherichia4
  • A pCb plasmid encoding a beta-lactamase from Haemophilus ducreyi was transferred to Escherichia coli, purified, and characterized. (lu.se)
  • New Delhi metallo-β-lactamase in Klebsiella pnemoniae and Escherichia coli, Canada. (flutrackers.com)
  • Multidrug-resistant Klebsiella pneumoniae and Escherichia coli isolates harboring New Delhi metallo-β-lactamase (NDM-1) were isolated from a patient who had returned to Canada from India. (flutrackers.com)
  • Acinetobacter calcoaceticus, Enterobacter aerogenes, Enterobacter cloacea, Escherichia coli, Haemophilus influenza (including ampicillin-resistant and beta-lactamase producing strains). (ibnhayyanpharma.com)
Pseudomonas1
  • A new cephalosporin with a highly reactive beta-lactam ring was found to give an immediate color change in the presence of beta-lactamases from many bacteria, including staphylococci, Bacillus species, Enterobacteriaceae, and Pseudomonas. (nih.gov)
Cephalosporins5
  • Beta-lactamases (β-lactamases) are enzymes (EC 3.5.2.6) produced by bacteria that provide multi-resistance to beta-lactam antibiotics such as penicillins, cephalosporins, cephamycins, monobactams and carbapenems (ertapenem), although carbapenems are relatively resistant to beta-lactamase. (wikipedia.org)
  • ESBLs are typically inhibitor-susceptible β-lactamases that hydrolyze penicillins, cephalosporins, and aztreonam and are encoded by mobile genes. (asm.org)
  • Amino acid sequencing of some beta-lactamases has shown that substitution of only a few amino acids in the bla gene leads to high-level resistance against specific cephalosporins. (lu.se)
  • Serine- and metallo-β-lactamases present a threat to the clinical use of nearly all β-lactam antibiotics, including penicillins, cephalosporins, and carbapenems. (ox.ac.uk)
  • Resistance develops much less frequently with fourth-generation cephalosporins because they are relatively stable to AmpC beta-lactamase but not (so far) to the less frequently encountered ESBLs (see Medical Care). (medscape.com)
Isolates5
  • During January--June 2010, three Enterobacteriaceae isolates carrying a newly described resistance mechanism, the New Delhi metallo-beta-lactamase (NDM-1) ( 1 ), were identified from three U.S. states at the CDC antimicrobial susceptibility laboratory. (cdc.gov)
  • Metallo-- lactamase (MBL) producing carbapenem resistant clinical isolates are an increasingly difficult problem in health care settings because of the treatment difficulties and rapid spread of these infections. (researchbib.com)
  • The present study aims in detecting the MBL production among carbapenem resistant clinical isolates.54 non repetitive clinical isolates of carbapenem resistant gram negative bacteria were studied to detect the Metallo-- lactamase production. (researchbib.com)
  • This study determined the prevalence of MDR A. baumannii isolates from intensive care units in a large tertiary-care hospital in Ismailia, Egypt, and the occurrence of different beta-lactamases in these isolates. (who.int)
  • Among the isolates, 6 isolates were carbapenemase producers, 2 were AmpC beta-lactamase producers and no isolates were metallo-beta- lactamase producers. (who.int)
Serine3
  • The structure of a Streptomyces serine β-lactamase (SBLs) is given by 1BSG. (wikipedia.org)
  • Serine β-lactamases are grouped by sequence similarity into types A, C, and D. The other type of beta-lactamase is of the metallo type ("type B"). Metallo-beta-lactamases (MBLs) need metal ion(s) (1 or 2 Zn2+ ions) on their active site for their catalytic activities. (wikipedia.org)
  • Confirmation that the OXA-2 beta-lactamase is a serine enzyme has come from the DNA sequence and the interaction with mechanism based inactivators. (bl.uk)
ENTEROBACTERIACEAE1
  • This is the first report of NDM-1 in the United States, and the first report of metallo-beta-lactamase carriage among Enterobacteriaceae in the United States. (cdc.gov)
Enzymes5
  • The gene for NDM-1 is one member of a large gene family that encodes beta-lactamase enzymes called carbapenemases. (wikipedia.org)
  • The carbapenems were developed to overcome antibiotic resistance mediated by bacterial beta-lactamase enzymes. (wikipedia.org)
  • A more detailed analysis has revealed from comparison of both primary and predicted secondary structure, that the OXA-2 beta-lactamase may be more closely related to class A enzymes. (bl.uk)
  • This domain is found in the RNase Z enzymes, which are closely related structurally to the Lactamase B family members. (embl.de)
  • P. aeruginosa also produces numerous antibiotic-inactivating enzymes such as β-lactamases, which target and break down β-lactam antibiotics. (uoguelph.ca)
Bacteria4
  • Beta-lactamases produced by gram-negative bacteria are usually secreted, especially when antibiotics are present in the environment. (wikipedia.org)
  • Although in the bacteria only beta-lactamases produce this color change, it was found that serum and tissues from experimental animals also rapidly produced the colored breakdown product, which was then excreted in the urine. (nih.gov)
  • Cefinase™ discs are intended for use in rapid testing of isolated colonies of Neisseria gonorrhoeae, Staphylococcus species, Haemophilus influenzae, enterococci and anaerobic bacteria for the production of β-lactamase. (thomassci.com)
  • They are more active against lactamases produced by bacteria. (howmed.net)
Beta-lactamase gene3
  • A specific DNA probe was designed to come entirely from within the coding sequence of the OXA-2 beta-lactamase gene. (bl.uk)
  • Another probe which comprised the whole of the OXA-2 beta-lactamase gene as well as segments of DNA on either side of the gene, was found to hybridise with a number of resistance plasmids. (bl.uk)
  • The sequence of the beta-lactamase gene was also determined. (lu.se)
Characterization1
  • Characterization of a new metallo-β-lactamase gene, bla NDM-1 , and a novel erythromycin esterase gene carried on a unique genetic structure in Klebsiella pneumoniae sequence type 14 from India. (cdc.gov)
Resistance8
  • Beta-lactamase provides antibiotic resistance by breaking the antibiotics' structure. (wikipedia.org)
  • However, BLA efficacy is declining due to resistance mechanisms including the widespread occurrence of β-lactamases, which catalyse β-lactam hydrolysis. (europa.eu)
  • Metallo-β-lactamases catalyze the hydrolysis of a broad range of β-lactam antibiotics and are a concern for the spread of drug resistance. (rcsb.org)
  • It confirmed that the enzyme belonged to a class A beta-lactamase which had 99% identity to the ampicillin resistance transposon Tn3 of pBR322. (lu.se)
  • Distribution of β -lactamases A and B in some groups of Yersinia enterocolitica and their role in resistance. (microbiologyresearch.org)
  • Beta-lactamases are the greatest single source of resistance to beta-lactam antibiotics. (uea.ac.uk)
  • For over 60 years, clinicians have seen a pattern whereby useful new beta-lactam analogues are introduced but then select for new beta-lactamases that cause resistance. (uea.ac.uk)
  • These results show that PA2915 and PA0832 possess low level β-lactamase activity but do not provide any significant resistance against β-lactam antibiotics in P. aeruginosa. (uoguelph.ca)
Hydrolysis2
  • Through hydrolysis, the enzyme lactamase breaks the β-lactam ring open, deactivating the molecule's antibacterial properties. (wikipedia.org)
  • The 3-D structure of Bacillus cereus (569/H/9) beta-lactamase (EC3.5.2.6), which catalyses the hydrolysis of nearly all beta-lactams, hasbeen solved at 2.5 A resolution by the multiple isomorphous replacementmethod, with density modification and phase combination, from crystals ofthe native protein and of a specially designed mutant (T97C). (embl.de)
Strains3
  • The reaction is confined to organisms producing beta-lactamases, but it is sufficiently sensitive to indicate the presence of this enzyme is small amounts in strains previously considered not to produce it. (nih.gov)
  • The β -lactamase isoelectric focusing patterns of 37 strains of Yersinia enterocolitica from various serological and biochemical groups were examined. (microbiologyresearch.org)
  • All strains now produce beta-lactamase. (msdmanuals.com)
Enzyme4
  • However, the blaNDM-1 gene produces NDM-1, which is a carbapenemase beta-lactamase - an enzyme that hydrolyzes and inactivates these carbapenem antibiotics. (wikipedia.org)
  • Characterisation of the physical properties of the OXA-2 enzyme by analytical ultracentrifugation have not only confirmed the dimeric nature of this beta-lactamase but have also shown that this enzyme forms aggregates at high protein concentrations. (bl.uk)
  • Probing of the enzyme structure with trypsin, strongly points to the OXA-2 beta-lactamase having a domain structure. (bl.uk)
  • Nevertheless, it should be recognized that the first β-lactamase enzyme produced by a Gram negative pathogen was discovered back in 1960 in Greece (Munoz-Price, 2019). (assignology.com)
AmpC1
  • AmpC mais aucun isolat ne produisait de métallo-bêta-lactamases. (who.int)
Metallo beta4
  • The structure of the New Delhi metallo-beta-lactamase 1 is given by 6C89. (wikipedia.org)
  • The 3-D structure of a zinc metallo-beta-lactamase from Bacillus cereusreveals a new type of protein fold. (embl.de)
  • A sequence comparison of the relevant metallo-beta-lactamases,based on this protein structure, highlights a few well-conserved aminoacid residues. (embl.de)
  • The binding sites of Sodium atom in the Metallo-Beta-Lactamase (pdb code 2bmi ). (atomistry.com)
Hydrolyze1
  • The beta-lactamase could hydrolyze both penicillin antibiotics including ampicillin, benzylpenicillin, and carbenicillin as well as cephalosporin antibiotics including nitrocefin, cephalothin, cephaloridine, and cefoperazone. (lu.se)
Penicillins1
  • Penicillinase is a specific type of β-lactamase, showing specificity for penicillins, again by hydrolysing the β-lactam ring. (wikipedia.org)
Detection3
  • Use of Intralactam for the early detection of staphylococcal beta-lactamase. (bmj.com)
  • The use of analytical isoelectric focusing for detection and identification of β -lactamases. (microbiologyresearch.org)
  • This rapid test is used for detection of Beta-lactamase in milk based on the colloidal gold immunochromatography technology. (bioeasy.com)
Carbapenemase1
Haemophilus2
  • Is β-Lactamase Testing Acceptably Accurate for Predicting Haemophilus " by Arianna B. Morton, Chairut Vareechon et al. (jefferson.edu)
  • Is β-Lactamase Testing Acceptably Accurate for Predicting Haemophilus Influenzae Susceptibility to Β-lactams? (jefferson.edu)
Gene3
  • The DNA sequence of the gene coding for the OXA-2 beta-lactamase has been completed. (bl.uk)
  • Whole-genome sequencing (WGS) was used for β-lactamase gene identification. (udayton.edu)
  • We used a cell-based human FXR β-lactamase ( Bla ) reporter gene assay to profile the Tox21 10K compound collection of environmental chemicals and drugs. (nature.com)
Chromogenic1
  • In addition, the target proteins were biochemically characterized in vitro by detecting β-lactamase activity using the standard chromogenic β-lactam substrate, nitrocefin. (uoguelph.ca)
Carbapenemases1
  • A total of 50 MDR-GNB, of which 29 carried multiple β-lactamases, underwent broth microdilution (BMD) and disk diffusion (DD) testing as well as confirmation tests for ESBLs and carbapenemases. (udayton.edu)
Proteins6
  • Apart from the beta-lactamases a number of other proteins contain this domain ( PUBMED:7588620 ). (embl.de)
  • There are 264030 Lactamase_B domains in 263820 proteins in SMART's nrdb database. (embl.de)
  • Taxonomic distribution of proteins containing Lactamase_B domain. (embl.de)
  • The complete taxonomic breakdown of all proteins with Lactamase_B domain is also avaliable . (embl.de)
  • Click on the protein counts, or double click on taxonomic names to display all proteins containing Lactamase_B domain in the selected taxonomic class. (embl.de)
  • Through proteomic analysis of the P. aeruginosa laboratory strain PAO1, a number of uncharacterized proteins were identified that are predicted to be novel β-lactamases. (uoguelph.ca)
Genes1
  • The beta-lactamase genes bla TEM-1 (40%) and bla CMY-2 (2.5%) were also identified. (biomedcentral.com)
Protein1
  • Livermore, DM 2009, ' beta-Lactamases- the Threat Renews ', Current Protein and Peptide Science , vol. 10, no. 5, pp. 397-400. (uea.ac.uk)
Plasmid1
  • Analytical isoelectric focusing of R factor-determined β -lactamases: correlation with plasmid compatibility. (microbiologyresearch.org)
Inhibition1
  • Chromophore-linked substrate (CLS405): probing metallo-β-lactamase activity and inhibition. (ox.ac.uk)
Mechanisms1
Inhibitor2
  • Cephalosporin Antibiotic + B-lactamase inhibitor. (ibnhayyanpharma.com)
  • Sulbactam in this combination is a potent, highly specific inhibitor of a wide variety of beta-lactamases produced by common gram-negative and gram-positive aerobes and anaerobes. (ibnhayyanpharma.com)
Genetic1
Class2
  • It appeared initially in a limited number of bacterial species (E. cloacae, C. freundii, S. marcescens, and P. aeruginosa) that could mutate to hyperproduce their chromosomal class C β-lactamase. (wikipedia.org)
  • no good homologies were observed with any class of beta-lactamase. (bl.uk)