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LaccaseSubstrate SpecificityIsoenzymesMolecular Sequence DataKineticsPolyporaceaeAmino Acid SequenceOxidoreductasesToxicodendronPleurotusBasidiomycotaCatalysisTrametesAscorbate OxidaseModels, MolecularPolyporalesBinding SitesHydrogen-Ion ConcentrationLigninSequence Homology, Amino AcidCatalytic DomainRecombinant ProteinsEscherichia coliCloning, MolecularBase SequenceCrystallography, X-RayHydrolysisEnzyme StabilitySequence AlignmentOxidation-ReductionProtein BindingTamarindusProtein ConformationMutagenesis, Site-DirectedProtein Structure, TertiarySulfonic AcidsElectrophoresis, Polyacrylamide GelBacterial ProteinsBenzyl AlcoholsMutationStructure-Activity RelationshipGlycoside HydrolasesCatechol OxidaseMolecular WeightCoumarinsCytochrome P-450 Enzyme SystemCopperLiverCulture MediaTemperatureShiitake MushroomsMicrosomes, LiverGene Expression Regulation, EnzymologicEscherichia coli ProteinsPhylogenySaccharomyces cerevisiaeFungal ProteinsAdenosine TriphosphateBenzothiazolesAmino AcidsDNA, ComplementarySordarialesEnzyme InhibitorsDNA PrimersChromatography, High Pressure LiquidEnzyme ActivationPhosphorylationMolecular StructureAcetatesModels, BiologicalAlcohol OxidoreductasesGlutathione TransferaseProtein Structure, SecondaryBiological TransportOligopeptidesStereoisomerismCattle
SpecificityEnzymeStrainsDegradationActivityActive siteActivitiesRange of substratesAromaticFound
Specificity3
Enzyme3
  • Sequential submerged cultivation with different induction media as inducers of ligninolytic enzyme production by Pleurotus ostreatus strains was assessed by measuring laccase activities. (ncsu.edu)
  • The laccase enzyme of different P. ostreatus strains in the different media exhibited large differences, and the wild strain YAASM 0568 exhibited enhanced production of laccase compared to other cultivated strains. (ncsu.edu)
  • This separation of polar and non-polar regions facilitates protein-protein interactions between the enzyme and a large range of substrates. (wikipedia.org)
Strains4
  • An unconventional material, alkali lignin, was used for the first time as an inducer for different strains to enhance laccase activity. (ncsu.edu)
  • The P. ostreatus strains secreted similar but relatively high levels of laccase activity when the induction media contained alkali lignin with or without glucose. (ncsu.edu)
  • The laccase activities of wild strain YAASM 0568 were nearly 3.4-, 3.3-, and 5.4-fold higher than that for cultivated strains CCMSSC 00322, CCMSSC 00406, and CCMSSC 00336, respectively, when the induction media contained alkali lignin, inorganic salt, and vitamin B1. (ncsu.edu)
  • Ability to degrade the herbicides containing dioxin and decolor type reactive dyes by laccase, laccase-like strains, and microbe strains were chosen to apply in the orientation in defense activities. (net.vn)
Degradation1
  • Therefore, the subject of study and research with the name "Research on the ability of decolorization of reactive dyes and degradation of herbicides/dioxin by microbes producing laccase" has been conducted. (net.vn)
Activity3
  • To increase our understanding and help engineer improved activity, we focused on the reactive, Michaelis complex M between MGL, covalently bound PLP, and substrate Met. (bvsalud.org)
  • 2012). Moreover, laccase activity has also been demonstrated in Basidiomycetes , Ascomycetes , and Deuteromycetes (Bourbonnais et al . (ncsu.edu)
  • Being not only high-activity laccase but also capable of strong activity, just together with liberal oxygen as well as the help of fastening substance or fastening systems (mediator). (net.vn)
Active site1
  • The Met ligand also stabilizes the correct orientation of the Schiff base, more strongly than in simulations without Met, and in agreement with structures in the Protein Data Bank, where the Schiff base orientation correlates with the presence or absence of a co-bound anion or substrate analogue in the active site. (bvsalud.org)
Activities1
  • C. antarcticus produced the highest laccase and Mn-peroxldase activities (33.0 and 1.6 U/g dry solid). (scielo.org.ar)
Range of substrates2
  • This separation of polar and non-polar regions facilitates protein-protein interactions between the enzyme and a large range of substrates. (wikipedia.org)
  • The wide range of substrates that can be attacked by laccases has led to a search for new sources of the enzyme (11,20,21). (scielo.br)
Aromatic2
  • The S2 subsite of zingibain contains the amino acid chain Trp67-Met68-Asn69-Thr133-Ala157, which makes the site too compact to accommodate larger hydrophobic aromatic substrate residues favored by other enzymes in the papain family. (wikipedia.org)
  • Laccases are oxidases that transfer electrons to dioxygen, yielding water with the concomitant oxidation of a wide range of reducing substrates, including phenolic compounds and aromatic amines. (scielo.br)
Found2
  • Recently, zingibain was found to exist as two isozymes, GP-I and GP-II, which were isolated by chromatography, with molecular weights of approximately 22,500 Da. (wikipedia.org)
  • Laccases have been found in many fungi, higher plants and insects (9,29) and are believed to be involved in several microbial and cellular events, such as fungal virulence (22), conidial pigmentation (31), lignification (6) and plant defense (18). (scielo.br)