Loading...
  • 1.10.3.2
  • Attributing to the polyphenolic structure of lignin, enzymatic modification of its surface using laccases (benzenediol:oxygen oxidoreductases, EC 1.10.3.2) has demonstrated to be highly successful. (osti.gov)
  • Laccases (EC 1.10.3.2, p -diphenol oxidase) are copper containing polyphenol oxidases that catalyze the oxidation of phenolic compounds with a concomitant reduction of molecular oxygen to water ( Thurston, 1994 ). (frontiersin.org)
  • 8 The copper-containing laccase ( p -diphenol oxygen oxidoreductase, EC 1.10.3.2) is able to catalyze the one-electron oxidation of phenolic substrates 9 that supply electrons to the laccase molecule for the four electron reduction of oxygen to water. (rsc.org)
  • enzyme
  • Application of Asymetrical and Hoke designs for optimization of laccase production by the white-rot fungus Fomes fomentarius in solid-state fermentation," Enzyme Research , vol. 2011, Article ID 368525, 12 pages, 2011. (hindawi.com)
  • 100, 2127-2139 (2006)) gives pH 6.5 as another value for the laccase from this species, with a range for enzyme activity of 4.3 to 9. (sigmaaldrich.com)
  • The purified enzyme oxidized some of the typical laccase substrates, including 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS), syringaldazine, and 2,6-dimethoxyphenol (2,6-DMP), along with pyrogallol and K 4 [Fe(CN) 6 ]. (springer.com)
  • As observed for other laccases, the enzyme was inhibited by halide anions and was sensitive to increasing concentrations of dimethyl sulfoxide and Tween-80. (springer.com)
  • Crude Laccase-assisted Decolorization of Synthetic Dyes-Approaches for a Cost-effective Enzyme-based Remediation Methodology," Applied Biochemistry and Biotechnology , vol. 169, no. 3, pp. 911-922, 2013. (hindawi.com)
  • In this study, laccase from Cerrena unicolor as a highly active enzyme was adsorptively immobilized on the surface of graphite electrode. (lu.se)
  • HYB07 yields a broth with laccase activity which when supplemented with distilled water and used as a destaining solution results in a clear gel background in 2 h at 37 °C. Also, separation of the laccase enzyme for efficient functioning is not required. (springer.com)
  • The combination of superheated steam (SHS) with ligninolytic enzyme laccase pretreatment together with size reduction was conducted in order to enhance the enzymatic hydrolysis of oil palm biomass into glucose. (mdpi.com)
  • fungus
  • The performed investigations demonstrate that laccase from white rot fungus C unicolor can be applied for the construction of biosensors useful for determination of flavonoids and related compounds in flow-injection system. (lu.se)
  • In this study, we designed three regulation strategies for laccase gene expression in the model fungus Aspergillus nidulans . (springer.com)
  • By introducing various promoters in front of the laccase gene pslcc from the white-rot fungus Pycnoporus sanguineus , we found that the laccase gene with the original promoter had effective expression in A. nidulans . (springer.com)
  • 2018
  • Barrios-Estrada C, de Jesús Rostro-Alanis M, Muñoz-Gutiérrez BD, Iqbal HMN, Kannan S, Parra-Saldívar R (2018) Emergent contaminants: Endocrine disruptors and their laccase-assisted degradation-a review. (springer.com)
  • Kumar G. (2018) Fungal Laccase Efficiently Destains Coomassie Brilliant Blue-R-250 Stained Polyacrylamide Gels. (springer.com)
  • recombinant
  • A recombinant two-domain laccase (SgfSL) was cloned from the genome of Streptomyces griseoflavus Ac-993, expressed in Escherichia coli and purified to homogeneity. (nih.gov)
  • Antosova Z, Sychrova H (2016) Yeast hosts for the production of recombinant laccases: a review. (springer.com)
  • Yeast hosts for the production of recombinant laccases: a review. (springer.com)
  • phenolic
  • Electrodes modified with laccase yield responses for both simple compounds (caffeic acid) and compounds with three or more phenolic and non-phenolic rings, although with different sensitivity. (lu.se)
  • Pyrolysis-GCMS ( pyr -GCMS) of the solid-state lignin residue after its treatment with laccase-mediator system (LMS) confirms significant enrichment of the solid-state lignin surface with phenolic groups. (rsc.org)
  • This study highlights an important role of the laccase in combination with a mediator methyl syringate for production of high-value phenolic monomers. (rsc.org)
  • isoenzymes
  • Amino acid sequences from peptides derived from digestion of isoenzymes showed great consistency with laccases in the databases. (mdpi.com)
  • inhibitory activity
  • J. Sun, H. Wang, and T. B. Ng, "Isolation of a laccase with HIV-1 reverse transcriptase inhibitory activity from fresh fruiting bodies of the Lentinus edodes (Shiitake mushroom)," Indian Journal of Biochemistry & Biophysics , vol. 48, no. 2, pp. 88-94, 2011. (hindawi.com)
  • Biosensor
  • Fu J, Qiao H, Li D, Luo L, Chen K, Wei Q. Laccase Biosensor Based on Electrospun Copper/Carbon Composite Nanofibers for Catechol Detection. (mdpi.com)
  • catechol
  • Laccase (Lac) based electrochemical biosensors offer a facile, simple, high-efficient analysis method for the detection of catechol, which is not affected by solution color and can achieve the microminiaturization of analysis apparatus. (hindawi.com)
  • The amperometric results indicated that the Cu/CNFs/laccase(Lac)/Nafion/glass carbon electrode (GCE) possessed reliable analytical performance for the detection of catechol. (mdpi.com)
  • assay
  • The automated chromatographic activity assay allowed for a defined reaction start of all laccase-mediator-system reactions mixtures, and the LMS reaction progress was automatically monitored for 48 h. (springer.com)
  • To standardize a procedure for the enzymatic assay of laccase at Sigma-Aldrich, St. Louis, Mo. (sigmaaldrich.com)
  • compounds
  • Integrity of laccase genes in anaerobes has been questioned, since laccases oxidize a variety of compounds using molecular oxygen as the electron acceptor. (springer.com)
  • Electrodes modified with laccase yield responses for both simple compounds (caffeic acid) and compounds with three or more. (lu.se)
  • Molecules
  • 5 One of the reasons is in large size of the laccase molecules, that cannot penetrate lignin pores and oxidize its internal surfaces. (rsc.org)
  • microorganisms
  • Their N termini were sequenced, revealing the same amino acid sequence and homology with laccases from other microorganisms. (csic.es)
  • yield
  • Glucose, in conjunction with a complex nitrogen source at pH 5.0, resulted in the highest laccase yield. (hindawi.com)
  • To improve the laccase yield, the dipeptidyl-peptidase DppV, aspartic protease PepA and mannosyltransferase Mnn9 were successfully deleted in the A. nidulans host. (springer.com)
  • alkaline
  • Strain UARK-01, a new thermophilic soil bacterium with hyperthermostable alkaline laccase activity. (springer.com)
  • Two-domain laccases have unusual thermostability, resistance to inhibitors and an alkaline optimum of activity. (nih.gov)
  • strain
  • Using the previously identified transcription factor RsmA regulatory mechanism, the aflR promoter was inserted into the pslcc expression vectors, and the laccase production was 15-fold higher in the strain overexpressing of RsmA compared to the control strain. (springer.com)
  • oxygen
  • To determine the optimal culture conditions for spore laccase production, the effects of Cu 2+ concentration, oxygen content, and culture time on spore laccase production from Bacillus pumilus W3 were investigated. (environmental-expert.com)
  • delignification
  • The use of laccases has been considered as a very powerful tool for delignification and detoxification of pretreated lignocellulosic materials, boosting subsequent saccharification and fermentation processes. (mdpi.com)
  • mediator
  • A comparative study of the enzymatically active systems with two laccases and a natural mediator applied to the alkali lignin has been performed. (rsc.org)
  • molecular
  • What is the molecular weight (MW) of Product L2157, Laccase from Rhus vernicifera? (sigmaaldrich.com)
  • 109, 6421-6432 (1987) reports estimated molecular weights of 52 kDa (laccase 1), 55 kDa (laccase 2), and 70 kDa (laccase 3), as determined by gel filtration. (sigmaaldrich.com)
  • The molecular weights of the purified laccases were estimated to be 68, 64 and 51 kDa, respectively. (mdpi.com)
  • Laccases LCC3 and LCC4 were characterized by SDS-PAGE, estimating their molecular masses around 80 and 82 kDa, respectively. (csic.es)
  • interestingly
  • Interestingly, copper induced laccase of 40 U/ml in culture medium was found to significantly induce cotton callogenesis. (frontiersin.org)
  • Interestingly, all the laccase isozymes were found to have an antioxidative role and therefore capable in free radicals scavenging during callogenesis. (frontiersin.org)