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Polymerase Chain ReactionClinical Laboratory TechniquesGelsLaboratoriesChromatography, High Pressure LiquidChromatography, AffinityChromatography, GelChromatographyChromatography, GasChromatography, Ion ExchangeChromatography, LiquidChromatography, Thin LayerSensitivity and SpecificityLaboratories, HospitalLimit of DetectionMolecular Sequence DataAnimals, LaboratoryChromatography, DEAE-CelluloseReproducibility of ResultsMolecular WeightMedical Laboratory PersonnelAmino Acid SequenceElectrophoresis, Polyacrylamide GelMass SpectrometryElectrophoresis, Gel, Two-DimensionalGas Chromatography-Mass SpectrometryKineticsChromatography, AgaroseBase SequenceElectrophoresis, Agar GelEvaluation Studies as TopicTime FactorsHydrogen-Ion ConcentrationChromatography, Reverse-PhaseClinical Laboratory Information SystemsElectrophoresis, Gel, Pulsed-FieldQuality ControlReference StandardsImmunoassayEscherichia coliCattleEnzyme-Linked Immunosorbent AssayIsoelectric FocusingDNA, BacterialSubstrate SpecificityAmino AcidsChromatography, PaperReagent Kits, DiagnosticSepharoseMethodsSpecies SpecificitySpecimen HandlingTandem Mass SpectrometryTemperatureRabbitsSpectrophotometry, UltravioletIndicators and ReagentsCalibrationCulture MediaBacterial ProteinsSpectrometry, Mass, Electrospray IonizationDNA PrimersSequence Analysis, DNALaboratory PersonnelFecesBiological AssayLiverDNAFalse Positive ReactionsChemical FractionationProtein BindingPeptide FragmentsRecombinant ProteinsLaboratory Proficiency TestingSwineChemistryElectrophoresisChemistry, ClinicalBacteriological TechniquesCell LineDrug StabilityMolecular Diagnostic TechniquesPeptidesVirologyChemical PhenomenaMutationRadioimmunoassayCloning, MolecularMagnetic Resonance SpectroscopyAlgorithmsBiosensing TechniquesAntibodies, MonoclonalMicrochemistryLaboratory Animal ScienceTrypsinBacteriaCarbohydratesHydrolysisChemistry Techniques, AnalyticalSubstance Abuse Detection
ElectrophoresisPermeation chromatographyExchange chromatographyMass SpectrometryHPLCPreparativeSilicaPurificationStationary phaseBiochemicalAffinitySeparation techniqueExtractionBiologicalQuantificationFlow cytometryQualitative analysisMembrane ProteinsAssaysMixturesChromatographic separation techniquesPhasesMethodsAnalysisPrincipleAnalyticalEnzymaticAnalysesLiquidQuantifyProteinsPolymerScientificPesticide residueInfectious diseasesResinHighDifferencesWorkChemicalInteractionApplicationsSample pretreatmentExperimentalProtein identificationEvaluationMeasurementLectureFermentationColumnClinicalSizeGenetic
Electrophoresis15
  • Simply mix with water, gently heat to dissolve the powder blend to quickly prepare reliable agarose gels for electrophoresis. (sigmaaldrich.com)
  • Agarose is non-toxic and has several properties and specifications that make it useful as a gelling agent in many applications, such as nucleic acid electrophoresis, immunodiffusion techniques, gel plates or overlays for cells in tissue culture, cell culture media, gel chromatography, affinity chromatography, and ion exchange chromatography. (sigmaaldrich.com)
  • Gel electrophoresis is a common life science laboratory technique used to extract biological molecules based on their size, such as in DNA separation and DNA detection. (sigmaaldrich.com)
  • Novotny was recognized for the development of PAGE Polyacrylamide Gel-filled Capillaries for Capillary Electrophoresis in 1993. (wikipedia.org)
  • Most notably, Milos has worked a great deal with microcolumn separation techniques of liquid chromatography, supercritical fluid chromatography, and capillary electrophoresis. (wikipedia.org)
  • Serum or Plasma Sample Preparation for Two-Dimensional Gel Electrophoresis - Anthony G. Sullivan, Stephen Russell, Henry Brzeski, Richard I. Somiari and Craig D. Shriver 7. (nhbs.com)
  • Large-Format Two-Dimensional Polyacrylamide Gel Electrophoresis - Henry Brzeski, Stephen Russell, Tony G. Sullivan, Richard Somiari & Craig Shriver 14. (nhbs.com)
  • Previous proteomic studies of intact platelets have collectively identified hundreds of proteins using a variety of fractionation strategies including 2-dimensional electrophoresis (2DE), multidimensional chromatographic separations, membrane prefractionation techniques, and adsorption to combinatorial hexapeptide ligand libraries [ 1 - 6 ]. (hindawi.com)
  • Polyacrylamide gel electrophoresis in the presence of SDS. (uc3m.es)
  • It offers extremely high flow and is recommended for both electrophoresis of large molecules and as a blotter to complete the blotting sandwich in gel transfer applications. (labfilter.com)
  • Ahlstrom manufactures a complete line of high quality chromatography electrophoresis and blotting papers for use in gel transfer applications and chromatography techniques. (labfilter.com)
  • Ahlstrom chromatography electrophoresis and blotting papers are made from high purity and 100% FDA compliant alpha cotton cellulose using ultra-pure RO water and contain no additives that could cause increased backgrounds or other interference in detection systems. (labfilter.com)
  • Two original products, Eprex (epoetin alfa) and Dynepo (epoetin delta), and two biosimilar products, Binocrit (epoetin alfa) and Retacrit (epoetin zeta), were compared using (1) high performance size exclusion chromatography, (2) ELISA, (3) SDS-PAGE, (4) capillary zone electrophoresis and (5) in-vivo potency. (springer.com)
  • Because a limited amount of EPO products was available, we were restricted to the common assays used for quality control: high performance size exclusion chromatography (HP-SEC) to assess monomer and aggregate content, ELISA to determine EPO protein content, SDS-PAGE under non-reducing conditions to detect possible fragmentation and covalent protein aggregates, capillary zone electrophoresis (CZE) for isoform profiling and a normocythemic mouse assay to test for potency. (springer.com)
  • A novel gel electrophoresis technique has reduced the time for the sample pretreatment of trace marker proteins derived from clinical samples from around 20 to 5 hours. (spectroscopyeurope.com)
Permeation chromatography30
Exchange chromatography3
  • In the first dimension, ion-exchange chromatography (IEC) separates peptides based on ionic interactions with the solid phase and an increasing salt buffer. (hindawi.com)
  • Chromatographic techniques based on charge (ion exchange chromatography). (uc3m.es)
  • In silico exercise addressing potential problems concerning the detection of heterologously expressed membrane proteins, solubilisation and evaluation of detergent properties, ion exchange chromatography and gel filtering in the presence of a detergent, and control of the protein's stability and integrity after purification. (lu.se)
Mass Spectrometry12
HPLC6
Preparative1
Silica6
  • During this procedure serum lipids are degraded in the sulfuric acid layer while cholesterol is removed in the top layer consisting of activated silica gel. (cdc.gov)
  • Without the activated silica gel layer cholesterol would eliminate water forming cholestene when coming in contact with the sulfuric acid. (cdc.gov)
  • various drawbacks have been identified in the purification techniques of ion resin adsorption ( 12 , 13 ), silica gel column chromatography ( 14 ) and supercritical extraction ( 15 ). (spandidos-publications.com)
  • HPTLC plates silica gel 60 F 254 (Merck), 20 x 10 cm are used. (camag.com)
  • In this technique, a phase-coated fused silica fiber is exposed to the headspace above the liquid or solid sample. (utm.my)
  • In TLC, the stationary phase is a thin layer of a solid adsorbent (like silica gel) on a flat surface, and the mobile phase is a liquid. (cannabissciencetech.com)
Purification7
  • Chromatography can be used in a myriad of applications from analyzing miniscule samples to production-scale use as a purification step. (coleparmer.com)
  • Lectures dealing with methods for theoretical modelling of membrane protein structure, fusion protein techniques, X-ray crystallography, heterologous expression, solubilisation and purification of membrane proteins are also included in the course. (lu.se)
  • Additional studies were undertaken to better understand the chromatographic behavior of PEGylated proteins in an effort to improve purification and characterization techniques of such proteins. (chromatographyonline.com)
  • Barnstead MicroPure Laboratory Water Purification System - Provides Type I Ultrapure Water. (apswater.com)
  • This article explores chromatographic separation techniques for the efficient purification of cannabinoids along with the benefits and drawbacks of these separation methods. (cannabissciencetech.com)
  • Column chromatography can be used as a large-scale method for purification and separation of the plant extract chemical profile. (cannabissciencetech.com)
  • It's one of the most fundamental and widely used methods in chemical and biochemical laboratories for purification purposes. (cannabissciencetech.com)
Stationary phase8
  • At its core, chromatography is the interaction between a mobile phase (which carries the mixture being separated) and a stationary phase (which performs the separation). (coleparmer.com)
  • Most chromatography methods have an inert mobile phase that carries the analyte through a long stationary phase housed inside a column. (coleparmer.com)
  • In size exclusion chromatography, the stationary phase is. (web.app)
  • The stationary phase is Polyethylene Glycol (PEG) in a Sol-Gel matrix. (utm.my)
  • In this technique, the stationary phase is a solid, and the mobile phase is a liquid. (cannabissciencetech.com)
  • In this technique, the stationary phase is packed into a column, and the mobile phase is allowed to flow through it. (cannabissciencetech.com)
  • Column chromatography is a separation technique used to separate and purify components from a mixture based on their different interactions with a stationary phase and a mobile phase. (cannabissciencetech.com)
  • The technique involves packing a column with a stationary phase material and passing a mobile phase (solvent) through it, which carries the mixture to be separated. (cannabissciencetech.com)
Biochemical1
  • DLS offers, again, free-- three free-- e-learning courses related to genetic testing, good laboratory practices for molecular genetic testing, good laboratory practice recommendations for biochemical, genetic testing, pre-analytic phase, and establishment and validation of new tests using liquid chromatography, and biochemical genetic laboratories. (cdc.gov)
Affinity1
  • Affinity chromatography - Spectrophotometric techniques: basic concepts and general principles. (uc3m.es)
Separation technique3
  • separation technique. (camag.com)
  • Chromatography, a widely utilized separation technique, offers promising solutions to overcome these challenges. (cannabissciencetech.com)
  • Chromatography is a versatile and widely used separation technique employed to separate and analyze mixtures of chemicals or substances into their individual components. (cannabissciencetech.com)
Extraction2
  • 1990. Utilization of solid-phase extraction and reversed-phase and ion- pair chromatography in the analysis of seven agrochemicals in water. (cdc.gov)
  • SPME is simple, can work with small sample sizes, solvent-free extraction technique that provides high sensitivity and excellent reproducibility. (utm.my)
Biological2
  • This technique is used extensively in the assay of ethanol and other solvents in blood and biological fluids as no sample preparation required. (utm.my)
  • A lateral flow immunoassay for the detection of anti-drug antibodies against a biological drug includes a membrane having a capture area, a sample application area, a flow path from the sample application area to the capture area, and a conjugate area located in the flow path. (justia.com)
Quantification1
  • These assays and kits can be used for various applications, such as mutation detection, gene expression, or residual DNA quantification. (excedr.com)
Flow cytometry2
  • This position will also involve developing novel fluorescent reagents for various detection platforms such as flow cytometry, microscopy, and live cell imaging systems. (biotium.com)
  • Besides equipment, Bio-Rad also provides kits, reagents, and consumables often needed by life science laboratories , such as flow cytometry antibodies, ELISA reagents, and even SARS-CoV-2 ddPCR kits . (excedr.com)
Qualitative analysis1
  • Trace evidence and fingerprint detection require an understanding of computerized quantitative and qualitative analysis, sampling, and spectroscopy. (ncsce.net)
Membrane Proteins1
Assays2
  • Their systems, instruments, and assays are fitted to the needs of life science laboratories that are focused on a range of varying applications. (excedr.com)
  • Current multiplex assays employ gel-based formats in which products are distinguished by size, fluorescent reporter dyes that vary in color, or secondary enzyme hybridization assays. (cdc.gov)
Mixtures2
  • Pre-Fractionation of Complex Protein Mixtures for 2-D PAGE Using Reversed Phase Liquid Chromatography Volker Badock and Albrecht Otto 12. (nhbs.com)
  • Chromatography is a technique for separating mixtures into their constituent parts. (coleparmer.com)
Chromatographic separation techniques1
  • Dr. Kashyap Thummar, Assistant Professor at Graduate School of Pharmacy (GSP), Gujarat Technological University (GTU), India, employs chromatographic separation techniques, especially HPTLC, to develop new and improved quantitative analytical methods for determination of drugs, impurities, adulteration and naturally occurring compounds in a variety of sample matrices. (camag.com)
Phases2
  • Regardless of how it is used, the overall effectiveness of chromatography largely depends on choosing the right technique and phases to use within that technique. (coleparmer.com)
  • Gel permeation chromatographysize exclusion chromatography as we have seen, gpcsec is a type of lc and so solid stationary and liquid mobile phases are again used. (web.app)
Methods4
  • Hands-on researchers describe in step-by-step detail 73 proven laboratory methods and bioinformatics tools essential for analysis of the proteome. (nhbs.com)
  • All laboratory-based protocols follow the successful Methods in Molecular BiologyT series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. (nhbs.com)
  • The review commenced by explaining the choice of paper, fabrication methods, and detection techniques to realize microfluidic lab-on-paper devices. (encyclopedia.pub)
  • All methods are based on the use of enzymes either purified by conventional chromatography or produced using molecular biology techniques. (megazyme.com)
Analysis7
  • Serum specimens are processed, stored, and shipped to the Division of Environmental Health Laboratory Sciences, National Center for Environmental Health, Centers for Disease Control and Prevention for analysis. (cdc.gov)
  • The course involves three hours of interactive lecture and discussion and three hours of laboratory work each week where students experience trace evidence collection, fingerprinting, ballistic analysis, blood-typing, and DNA fingerprinting. (ncsce.net)
  • Protein and peptide chromatography prior to MS analysis can partly address this issue. (hindawi.com)
  • Improvements at this stage may be especially important, since lapses here may be beyond correction at subsequent stages, which include the generation and detection of target fragments by MS, as well as the analysis of MS data by computational systems. (genengnews.com)
  • For headspace mode of analysis, it permits the detection of volatile substances in a liquid or solid sample and minimizes column contamination. (utm.my)
  • The clenbuterol urinalysis testing process involves various stages, including sample collection, transportation, and laboratory analysis. (ligadebolsa.com)
  • The gas chromatography-mass spectroscopy analysis was performed for the isolated active components exhibited antimicrobial activity. (ijpsonline.com)
Principle1
  • Today, most applications of chromatography are generally less colorful but the underlying principle of separation remains unchanged. (coleparmer.com)
Analytical2
  • These standards are characterized using a battery of analytical tests, including gpc, gpc with light scatter ing detection. (web.app)
  • For this September issue of The Application Notebook should be reaching our audience of analytical chemists, lab managers, and other industry professionals just as the busiest time of the year ramps up in the world of chromatography. (chromatographyonline.com)
Enzymatic2
  • 1995. Validation of an enzymatic biosensor with liquid chromatography for pesticide monitoring. (cdc.gov)
  • A collaborative study was conducted involving 8 laboratories (including the authors' laboratories) to validate the streamlined enzymatic method for determination of β-D-glucan in barley and oats. (megazyme.com)
Analyses1
  • The bioautography of TLC chromatogram showed single band exhibited antibacterial activity and phytocompound from this band was isolated and identified as 5,6,7,8-tetrahydro-1,2,4-benzotriazine-3-amine using gas chromatography-mass spectroscopy analyses. (ijpsonline.com)
Liquid3
Quantify1
  • For example, chromatography can be used to quantify how much pesticide residue was present in a batch of apple juice or determine how much of an active drug is present in a pill. (coleparmer.com)
Proteins3
  • A previous study from our laboratory using a MuDPIT (multidimensional protein identification technology) approach identified over 300 proteins secreted by platelets upon thrombin activation [ 1 ]. (hindawi.com)
  • Yet these techniques may inadvertently exclude or degrade proteins of interest. (genengnews.com)
  • And should a tighter focus be desired, there are techniques that can peer into niches that harbor elusive forms such as small peptides or post-translationally modified proteins. (genengnews.com)
Polymer2
  • Composed of semipermeable, porous polymer gel beads with well defined range of pore sizes. (web.app)
  • Different characterization techniques were used to determine polymer properties. (ndltd.org)
Scientific4
  • The course is organized around well-known criminal case-studies that highlight particular forensic techniques and the scientific content upon which they depend. (ncsce.net)
  • However, a lack of laboratory-based research has limited the scientific assessment of drug misuse in the Arabian Gulf region. (biomedcentral.com)
  • The course provides an up-to-date overview of the main techniques used in the field of biochemistry and presents how chemistry, physics and biology can be applied to scientific problems in the field of experimental sciences. (uc3m.es)
  • Bio-Rad aims to support scientific education, research, and discovery for laboratories across every industry. (excedr.com)
Pesticide residue1
  • The GC-MS and GC-FID techniques were employed in the determination of the pesticide residue. (scialert.net)
Infectious diseases1
  • Efficient laboratory diagnosis of infectious diseases is increasingly important to clinical management and public health. (cdc.gov)
Resin1
High2
  • Journal of High Resolution Chromatography 18(3): 194-195. (cdc.gov)
  • The Shimadzu GCMS-TQ8040 is the first Triple Quadrupole with Smart Productivity for high efficiency sample throughput, Smart Operation for quick and easy method development, and Smart Performance for low detection limits and Scan/MRM. (utm.my)
Differences1
  • By exploiting these differences, chromatography allows for the separation of compounds based on their various properties, such as size, charge, polarity, and interaction with specific chemical groups. (cannabissciencetech.com)
Work8
  • Practical laboratory classes and practices with computer media: students will carry out supervised experimental or computational work in specialized laboratories in which they will put into practice the theoretical knowledge acquired in the different subjects and learn to work safely in the laboratory. (uc3m.es)
  • What Is Chromatography & How Does It Work? (coleparmer.com)
  • The teaching consists of lectures, exercises, laboratory sessions and project work. (lu.se)
  • Compulsory participation is required in exercises, laboratory sessions, project work and associated elements. (lu.se)
  • Gel permeationsize exclusion chromatography 5 chapter 2 gpcsec overview 6 polymers 6 size matters 6 how does gpcsec work 7 who uses gpcsec, what for and why 8 calibrations 8. (web.app)
  • Summer research programs offered in conjunction with academic and research institutions may provide invaluable hands-on experience in laboratory work. (agt-info.org)
  • Work is carried out in a typical chemistry laboratory setting. (biotium.com)
  • Work may also require sitting and working at a computer or standing at a laboratory bench for long periods of time. (biotium.com)
Chemical1
Interaction1
  • Molecular modeling and docking technology, which is efficient, accurate and simple to use, can reproduce the true interaction process between drug and targets that some of the current laboratory techniques are unable to detect, and is a method for predicting the mechanism of action of a drug ( 19 ). (spandidos-publications.com)
Applications3
  • We provide a wide array of agarose products with varying gel strengths, melting temperatures, gelling temperatures, and electroendosmosis (EEO) levels to accommodate all of your research applications. (sigmaaldrich.com)
  • There are various types of chromatography techniques, each with its own principles and applications. (cannabissciencetech.com)
  • A Feature Paper should be a substantial original Article that involves several techniques or approaches, provides an outlook for future research directions and describes possible research applications. (mdpi.com)
Sample pretreatment1
  • Then, the sample pretreatment procedure used to improve the detection performance of lab-on-paper devices was introduced. (encyclopedia.pub)
Experimental4
  • Laboratory and discussion sessions stress problem solving and experimental design. (mnsu.edu)
  • SEMINARS: The seminars, given in smaller groups, will be aimed at : - Problem solving - Discussion of experimental assumptions - Commentary and discussion of some publications especially relevant to the development of some of the biochemistry and molecular biology techniques. (uc3m.es)
  • This laboratory manual provides an authoritative collection of core experimental procedures that underpin modern fission yeast research. (cshlpress.com)
  • In order to develop and validate these mathematical models, we collected experimental data in our own laboratories and also used experimental data available in the literature. (ndltd.org)
Protein identification1
  • The bioinformatics tools include those for analyzing 2D-GEL patterns, protein modeling, and protein identification. (nhbs.com)
Evaluation1
  • Evaluation of gel permeation chromatographic techniques. (web.app)
Measurement1
  • Types of radioactive emissions Units of radioactivity Detection and measurement of radioactivity. (uc3m.es)
Lecture1
  • Laboratory will reinforce lecture concepts. (mnsu.edu)
Fermentation1
  • 1993. Application of solid state fermentation techniques to dispose of chlorpyrifos and methachlor. (cdc.gov)
Column2
  • The sample was introduced into the electron ionization source either directly or via a gas chromatography column. (uochb.cz)
  • Theory of separation a column is made up of swollen gel particles and the solvent used to swell the gel in a suitable tubular container. (web.app)
Clinical1
  • Bio-Rad Laboratories, a developer and manufacturer in the life sciences industry, provides instruments, apparatus, software, consumables, and other materials necessary to assist the life science research and clinical diagnostics markets. (excedr.com)
Size1
Genetic2
  • It is anticipated that the panel will continue to evolve as the knowledge of genetic conditions expands, detection technologies advance, and new treatments emerge. (cdc.gov)
  • In addition, to the free e-learning courses, we also offer free job aids available in PDF and Word format, such as "Genetic Testing, What You Need to Know, Health Professionals," and "Genetic Testing, What You Need to Know," and the top 10 recommendations for laboratories performing molecular genetic testing. (cdc.gov)