Labelled nucleic acid probe blotting southern. Medical search. Frequent questions

The latter is done with antibodies or hybridization probes that bind only to some molecules of the blot and have an enzyme joined to them. (wikipedia.org)
DIG-labeled hybridization probes are highly sensitive and suitable for low-(single)-copy gene detection of rare mRNA in Southern and northern blots. (thomassci.com)
DNA-DNA hybridization probes were made from virulence factors amplified from O157:H7 EDL933 genomic DNA. (cdc.gov)

In many instances, this is done after a gel electrophoresis, transferring the molecules from the gel onto the blotting membrane, and other times adding the samples directly onto the membrane. (wikipedia.org)
Southern blotting combines transfer of electrophoresis-separated DNA fragments to a filter membrane and subsequent fragment detection by probe hybridization. (wikipedia.org)
Proteins are first separated by size using electrophoresis before being transferred to an appropriate blotting matrix (usually polyvinylidene fluoride or nitrocellulose) and subsequent detection with antibodies. (wikipedia.org)
The proteins are separated by gel electrophoresis and are subsequently transferred to nitrocellulose membranes similar to other types of blotting. (wikipedia.org)
Proteins are separated by gel electrophoresis before being transferred to a blotting matrix whereupon posttranslational modifications are detected by specific substrates (cholera toxin, concanavalin, phosphomolybdate, etc.) or antibodies. (wikipedia.org)
Northern blotting first separates samples by size via gel electrophoresis before they are transferred to a blotting matrix and detected with labeled RNA probes. (wikipedia.org)
A dot blot is a special case of any of the above blots where the analyte is added directly to the blotting matrix (and appears as a "dot") as opposed to separating the sample by electrophoresis prior to blotting. (wikipedia.org)
Dot- and slot-blot hybridization apply extracted dna from a sample directly onto a specific area (dot or slot) of filter paper, with the gel electrophoresis and transfer steps of southern hybridization being bypassed. (tainhatvip.net)
It represents a simplification of the western blot method, with the exception that the proteins to be detected are not first separated by electrophoresis. (tainhatvip.net)
It represents a simplification of the western blot method, with the exception that the proteins to be detected are not first separated by electrophoresis.Instead, the sample is applied directly on a membrane in a single spot, and the blotting procedure is performed. (web.app)
Often it is informative to quantify the abundance of a certain RNA or DNA in the extracted nucleic acid mixture by dot blot or slot blot hybridization without prior digestion and electrophoresis. (web.app)
The DNA is cleaved using a restriction endonuclease, and the pieces are separated by gel electrophoresis and are denatured and transferred (blotted) to a nitrocellulose membrane for analysis. (pharmacy180.com)
The App DNA Libraries MCQ e-Book PDF Download: dna technology applications, stem cells, gel electrophoresis and southern blotting, restriction enzymes test prep for pre employment screening tests. (mcqslearn.com)
Draw a neat labelled diagram of a typical agarose gel electrophoresis. (mcqexams.com)

DIG-labeled DNA probes have also been used for labeling of DNA during in situ hybridization. (thomassci.com)
Gene probes are used in various blotting and in situ hybridization (ISH) techniques for the detection of nucleic acid sequences in food industry, environmental, medical, and veterinary applications to improve the specificity of the analyses. (enzolifesciences.com)
Probes labeled by nick translation can be used in many different hybridization techniques including: chromogenic in situ hybridization (CISH), fluorescent in situ hybridization (FISH), screening gene banks by colony or plaque hybridization, DNA or RNA transfer hybridization, and re-association kinetic studies. (enzolifesciences.com)
In situ hybridization, slot blot, and southern blot hybridization analyses are all very effective in detecting the common hpv types (hpvs 6/11 or 16). (tainhatvip.net)
The biotin DNA probe labeled with this kit can be used for conventional Northern, Southern, colony or plaque hybridization, dot or slot blot, in situ hybridization, etc. (accegen.com)
Among the HPV types detected with the PATHO-GENE ® HPV screening probe are those commonly associated with benign lesions presenting as condylomas and mild dysplasia, as well as types commonly associated with cervical intraepithelial neoplasia (CIN) and carcinoma in situ (CIS). (enzolifesciences.com)
HPV probe can be optimized for polymer detection system using diluent buffer, In situ hybridization buffer for HPV probes (Ready-to-Use) (Prod. (enzolifesciences.com)
This review is about "molecular cytogenetics" including 1) the historical perspective of its development from cytogenetics, 2) technical aspects, 3) available probe sets, and 4) variants and applications of the basic fluorescence in situ hybridization (FISH) approach. (frontiersin.org)

The northern blot is for the detection of specific RNA sequences in complex samples. (wikipedia.org)
The reverse northern blot differs from both northern and Southern blot in that DNA is first immobilized on a blotting matrix and specific sequences are detected with labeled RNA probes. (wikipedia.org)
6). Detection of specific nucleic acid sequences by hybridization a. mini-prep of plasmid DNA b. (ntu.edu.tw)
The dot-blot hybridization is a nucleic acid hybridization technique where complementary single-stranded sequences of the probe (either rna or dna) hybridizes with single-stranded sequences of the test samples (either rna or dna) under suitable. (tainhatvip.net)
Hybridization analysis can then be carried out to determine the relative abundance of target sequences in the blotted dna preparations. (tainhatvip.net)
The technique is based on specific hybridization of small labeled DNA fragments, the probes, to complementary sequences in a target DNA molecule. (biomedcentral.com)
Southern blotting is a technique that can be used to detect specific sequences present in DNA. (pharmacy180.com)
Applications of the PCR technique include: 1) efficient comparison of a normal gene with a mutant form of the gene, 2) detection of low-abundance nucleic acid sequences, 3) forensic analysis of DNA samples, and 4) prenatal diagnosis and carrier detection (for example, of cystic fibrosis). (pharmacy180.com)

This product is designed as a safer chemical.Â The DIG System was established as a sensitive and cost-effective alternative to using radioactivity for the labeling and detection of nucleic acids. (thomassci.com)

A southwestern blot is based on Southern blot and is used to identify and characterize DNA-binding proteins by their ability to bind to specific oligonucleotide probes. (wikipedia.org)
Molecular probes can be broadly categorized into DNA probes and RNA probes, cDNA probes, and synthetic oligonucleotide probes. (enzolifesciences.com)
1990), the bbsh assay is attractive because it derives its sensitivity from the combined specificities of the detection oligonucleotide probe and the nucleic acid affinity support (ranki et al. (tainhatvip.net)

Similar to a western blot, the far-western blot uses protein-protein interactions to detect the presence of a specific protein immobilized on a blotting matrix. (wikipedia.org)
Antibodies are then used to detect the presence of the protein-protein complex, making the Far-Western blot a specific case of the Western blot. (wikipedia.org)
High-performance thin-layer chromatography is first used to separate the lipids by physical and chemical characteristics, then transferred to a blotting matrix before the oligosaccharides are detected by a specific binding protein (i.e. antibodies or lectins). (wikipedia.org)
Southern blot for DNA northern blot for RNA reverse northern blot for RNA western blot for proteins far-western blot for protein-protein interactions eastern blot for post-translational modification far-eastern blot for glycolipids dot blot Immunoscreening Towbin, Staehelin T, Gordon J, et al. (wikipedia.org)
The amino acid is added to a growing protein during the process of translation. (wikibooks.org)
secretase" which cleaves between amino acids 16 and 17 of the protein. (justia.com)
amyloid precursor protein (.beta.APP), which is expressed in differently spliced forms of 695, 751, and 770 amino acids. (justia.com)
Each dot or slot blot would contain known amounts of target protein or cell lysate. (web.app)
Category PROTEIN BIO- DOT (' SLOT BLOT ') PROTEIN BIO-DOT ('SLOT BLOT') Adapted from existing protocols by Vinh Pham. (web.app)
The IB1cDNA encodes a 714 amino acid protein with a proline-rich region and a putative basic helix-loop-helix domain (bHLH). (justia.com)
The current MCAT regularly has questions which require previous knowledge of the structures, features (including changes in charge with pH), 3- and 1-letter abbreviations of the 20 common protein-generating amino acids, etc. (mcat-prep.com)
A protein consists of one or more chains of amino acids (called polypeptides) whose sequence is encoded in a gene. (genome.gov)
Each time an amino acid is added to a growing polypeptide during protein synthesis, a tRNA anticodon pairs with its complementary codon on the mRNA molecule, ensuring that the appropriate amino acid is inserted into the polypeptide. (genome.gov)
Two compounds, Compound 1 (1,4-dihydrophenanthrolin-4-one-3-carboxylic acid) and Compound 5 [8-(N-butyl-N-ethylcarbamoyl)-1,4-dihydrophenathrolin-4-one-3-carboxylic acid], with comparable potencies in vivo, were chosen to investigate the effect of the inhibition of the hydroxylation of newly synthesized uterine collagen on the turnover of this protein in vivo. (embl.de)
Using a fluorescent sulfhydryl probe as well as a sulfhydryl inhibitor, one observes a strong cell density response, supporting the hypothesis that cellular redox potential could alter protein stability. (embl.de)
A small conserved open reading frame in the plastid genome, ycf9 , encodes a putative membrane protein of 62 amino acids. (silverchair.com)

Pair-wise comparison of three experimental conditions was obtained either with two labeled cDNA samples co-hybridized to the same array, or by employing Shewanella genomic DNA as a standard reference. (biomedcentral.com)
An alternative approach to the identification of the murine homologue was to use cross-species DNA hybridization, employing human CD2 cDNA as a probe. (ox.ac.uk)
The murine cDNA sequence encoded a predicted mature polypeptide of 322 amino acids that showed 54% identity with the predicted human sequence. (ox.ac.uk)
A rabbit antiserum raised against purified human CD2, precipitated from surface-labeled mouse thymocytes a glycoprotein of Mr 55,000-66,000 which decreased to Mr 35,000 on digestion with endo-beta-acetylglucosaminidase F. These sizes are consistent with those predicted for the murine CD2 antigen from the cDNA sequence. (ox.ac.uk)

A blot, in molecular biology and genetics, is a method of transferring proteins, DNA or RNA onto a carrier (for example, a nitrocellulose, polyvinylidene fluoride or nylon membrane). (wikipedia.org)
After the blotting, the transferred proteins, DNA or RNA are then visualized by colorant staining (for example, silver staining of proteins), autoradiographic visualization of radiolabelled molecules (performed before the blot), or specific labelling of some proteins or nucleic acids. (wikipedia.org)
A western blot is used for the detection of specific proteins in complex samples. (wikipedia.org)
The eastern blot is used for the detection of specific posttranslational modifications of proteins. (wikipedia.org)
A dot blot (or slot blot) is a technique in molecular biology used to detect proteins. (tainhatvip.net)
The 96-well Bio-Dot ® and 48-well Bio-Dot SF (slot format) microfiltration units provide easy, reproducible methods for binding proteins or nucleic acids in solution onto membranes. (web.app)
An amino acid is the fundamental molecule that serves as the building block for proteins. (genome.gov)
HIV infection can be diagnosed based on detection of antibodies that are directed against the proteins encoded by the 3 major genes, the detection of the p24 antigen, the viral nucleic acid, and, finally, by means of culturing the virus. (medscape.com)

For convenient and efficient generation of DIG-labeled DNA probes in polymerase chain reaction (PCR). (thomassci.com)
Culture is the most common diagnostic test for gonorrhea, followed by the deoxyribonucleic acid (DNA) probe and then the polymerase chain reaction (PCR) assay and ligand chain reaction (LCR). (medscape.com)

It usually only takes a few minutes, slot blot hybridization. (tainhatvip.net)
The credits and jackpot values displayed are strictly simulations with no cash or prize value, slot blot hybridization. (tainhatvip.net)
Related products: deionized formamide For example, Thanks again for the great customer service, slot blot hybridization. (tainhatvip.net)
Winning on video slots is all about luck, and every player should be aware of that, slot blot hybridization. (tainhatvip.net)
Slot blot hybridization performed on dna of tissue specimens from 16 patients revealed that biopsy tissue from 3 (19 percent) contained ebv dna. (tainhatvip.net)

If a complementary sequence is searched for in a mixture of blotted mRNA s in a completely analogous way, it is called Northern blotting . (wikilectures.eu)
The DNA probe hybridizes with a specific mRNA, if present. (musc.edu)

Fusing of two complementary strands of nucleic acids in vitro into double helices is called reassociation . (wikilectures.eu)
In practice, double and single stranded DNAs, mRNAs, and other RNAs synthesized in vitro are all used as probes. (enzolifesciences.com)
The short-term fate of the endocytosed pDNA in vivo and in vitro was investigated by Southern blot. (silverchair.com)

First, ssDNA with a known sequence, complementary to the nucleic acid sought in the mixture, is attached to e.g. a membrane filter or a carbohydrate gel in the column. (wikilectures.eu)
In the procedure, the nucleic acid mixture is blotted to a membrane where the hybridization is carried out. (web.app)

Electrophoretic fractions (DNA fragments) are blotted onto a nitrocellulose filter and denatured. (wikilectures.eu)

During the flow of the investigated mixture of nucleic acids through a filter or column modified in this way, the sought complementary ssDNA or ssRNA associates with the DNA fixed to the carrier. (wikilectures.eu)
This so-called probe associates only with a DNA restriction fragment containing a complementary sequence, which will be shown by the attached photographic film ( autoradiography ) after rinsing the filter. (wikilectures.eu)
A probe is a small piece of RNA or single-stranded DNA (usually labeled with a radioisotope, such as 32P, or another recognizable compound, such as biotin or a fluorescent dye) that has a nucleotide sequence complementary to the DNA molecule of interest (target DNA). (pharmacy180.com)

This robust enzyme mix with proofreading activity will polymerize probes 40 bp to 5 kb long using 10 pg plasmid DNA and 10 ng genomic DNA as template. (thomassci.com)
Amplification products are assayed in genomic Southern blots. (thomassci.com)
The gene encoding the Ov-phy-1 open reading frame contained 11 introns, similar in structure to the gene encoding human prolyl 4-hydroxylase isoform I. Genomic Southern blot, EST and genomic PCR studies demonstrated that the O. volvulus genome contained between three and eight genes closely related to Ov-phy-1. (embl.de)
From one of the projects involving the preparation of recombinant mice, we selected 45 genomic DNA extracted from ES clones that then underwent Southern blot screening. (biomedcentral.com)

Dot/Slot (Filtration) Blotting: Western Blot Dot blotting is an ideal technique for quickly assessing the levels of a target antigen across many samples at once. (web.app)

In particular, the use of IB1 nucleic acid, IB1 polypeptides and anti-IB1 antibodies in the diagnosis, and prophylactic and therapeutic treatment of conditions such as diabetes and neurological diseases such as dementia and/or parkinsonism is disclosed. (justia.com)
In particular, the present invention relates to the uses of IB1 nucleic acid, IB1 polypeptides and anti-IB1antibodies in the diagnosis, and prophylactic and therapeutic treatment of conditions such as diabetes and neurological diseases such as dementia and/or parkinsonism. (justia.com)

This kit can be used to label various DNA templates, including DNA fragments, linearized plasmids, supercoiled DNA, etc. (accegen.com)

Southern and Northern blots and reverse-transcription PCR analyses indicate that the same rhodopsin gene is expressed in the retina and the brain but not in the pineal organ of ayu. (bioone.org)

Rapid identification of up to three Candida species in a single reaction tube by a 5' exonuclease assay using fluorescent DNA probes. (musc.edu)

Nucleic acid probes are either a single stranded DNA or RNA with a strong affinity towards a specific DNA or RNA target sequence. (enzolifesciences.com)

Immunochemists continued this joke: in western blotting , it is an analogous technique, but the antigen interacts with the antibody. (wikilectures.eu)
The DNA probe is an antigen detection test that uses a probe to detect gonorrhea DNA in specimens. (medscape.com)

It is an intermediate step in the biological synthesis of cholesterol from mevalonic acid in vertebrates. (lookformedical.com)
Each lot of the PCR DIG Probe Synthesis Kit is function tested in PCR. (thomassci.com)
Furthermore, the use of Taq or other thermostable DNA polymerases permits labeling reactions to be performed at higher temperatures via PCR, thereby reducing the incidence of enzyme-mediated point mutations during probe synthesis. (enzolifesciences.com)
PCR is an excellent method for probe synthesis, requiring very small quantities of template material. (enzolifesciences.com)
Alternatively, the primers themselves may be labeled non-isotopically during their own synthesis, negating the requirement for the inclusion of labeled nucleotide precursors as part of the reaction mix. (enzolifesciences.com)
Regardless of the type of blot, the principles of probe synthesis, hybridization, washing and detection are the same. (web.app)

The structure bound to the substrate analogue along with mutagenesis of predicted critical amino acid residues led us to suggest a reaction mechanism distinct from all known NADases. (nature.com)
After proper washing, this enzymatic activity (and so, the molecules we search in the blot) is visualized by incubation with proper reactive, rendering either a colored deposit on the blot or a chemiluminescent reaction which is registered by photographic film. (wikipedia.org)
One PCR labeling reaction (50 Î¼l) will typically yield enough probe for 20 ml hybridization solution. (thomassci.com)
Nick translation is efficient for both linear and covalently closed DNA molecules, and labeling reaction are completed in less than an hour. (enzolifesciences.com)

Dot blot Method - YouTube Dec 05, 2011 · The efficiency of labeling can be estimated by comparing the relative detection sensitivity of the labeled nucleic acid to a standardized sample in a side by side dot blot dilution series. (web.app)

Briefly, a suspension containing a mixture of 2×10 6 lymphocytes and 2×10 6 of 1301 cells was denatured at 82°C for 10 min, with and without the fluorescein-conjugated PNA telomere probe in the hybridisation solution. (ersjournals.com)
The PATHO-GENE ® HPV screening probe is a mixture of biotin-labeled HPV 6, HPV 11, HPV16, HPV 18, HPV 31, HPV 33, and HPV 51-specific probes in buffered formamide and hybridization enhancers. (enzolifesciences.com)
The ENZO PATHO-GENE ® HPV screening probe employs a mixture of the HPV-specific probes that have been specificallly formulated to detect most HPV types that have been associated with anogenital papillomas. (enzolifesciences.com)
The probe mixture is denatured and hybridized to the tissue section fixed and pretreated on the microscope slide. (enzolifesciences.com)

Specific molecular probes and primers are designed for this purpose. (enzolifesciences.com)

Many new nucleic acid-based diagnostic tools or assays have been developed that allow analysis of DNA and RNA molecules in clinical samples. (enzolifesciences.com)
In theory, any nucleic acid can be used as a probe provided it can be labeled to permit detection and quantitation of the hybrid molecules formed between the probe and sequence to be identified. (enzolifesciences.com)
Next, the enzyme DNA polymerase I removes the native nucleotides from the probe molecules in the 5′→3′ direction (exonuclease activity) while replacing them with labeled dNTP precursors by virtue of its 5′→3′ polymerase activity. (enzolifesciences.com)

The derived amino acid sequence of Ov-phy-1 encoded a peptide that was most similar to the two Caenorhabditis elegans prolyl 4-hydroxylase homologues and to the isoform II enzymes of vertebrates. (embl.de)

Once dry, dot blots and slot blots are subjected to the same immunodetection steps used for Western blotting, i.e. blocking, antibody incubation, and target detection with substrate. (web.app)

fluorophore-labeled , biotin-labeled , and digoxigenin-labeled nucleotides. (enzolifesciences.com)
AceSeq™ Biotin Random Primer DNA Labeling Kit is designed for generating biotin-labeled DNA probes through random primer labeling reactions. (accegen.com)
AceSeq™ Biotin Random Primer DNA Labeling Kit that is free of hazardous chemicals has high efficiency. (accegen.com)
Conventional labels for the DNA probe include the radioisotope labels 32P and 125I and the chemical label biotin. (musc.edu)
Specific hybridization between the HPV DNA probe and DNA in the specimen is determined by the detection of biotin. (enzolifesciences.com)

The described method of DNA restriction fragment analysis was developed by Eduard M. Southern. (wikilectures.eu)
A DNA probe is a labeled fragment of DNA that contains a nucleotide sequence specific for the gene or chromosomal region of interest. (enzolifesciences.com)
The annealed primers ultimately become part of the probe itself, because the Klenow fragment of DNA polymerase I extends the primers in the 3′ direction and, in so doing, incorporates the label. (enzolifesciences.com)
The fragment of interest is detected using a probe. (pharmacy180.com)

A Southern blot is a method routinely used in molecular biology for detection of a specific DNA sequence in DNA samples. (wikipedia.org)
However, blotting is an elegant, fast and routinely manageable method. (wikilectures.eu)
We compared the results from Southern blot, routinely used to quantify NEO copies, with the two Real-Time PCR assays. (biomedcentral.com)

DNA stands for deoxyribonucleic Acid . (wikibooks.org)
Deoxyribonucleic acid that makes up the genetic material of fungi. (lookformedical.com)

DNA/RNA probe assays are faster and sensitive so that many conventional diagnostic tests for viruses and bacteria involving culturing of the organisms are being fast replaced by molecular probe assays. (enzolifesciences.com)
While culture tests can take days, molecular probe assays can be performed within a few hours or minutes. (enzolifesciences.com)
Conventional techniques used for testing for the hybridization product include dot blot assays, Southern blot assays, and DNA:RNA hybrid-specific antibody tests. (musc.edu)
During a recent work, where a knockin SCA28 mouse was prepared, we developed two assays based on Real-Time PCR using both SYBR Green and specific minor groove binder (MGB) probes to evaluate the copies of NEO using the comparative delta-delta Ct method versus the Rpp30 reference gene. (biomedcentral.com)

Uptake of 125 I-labelled pDNA in cultures of cod aEEC was specific. (silverchair.com)
The use of DNA probes provides a specific, sensitive, rapid, and inexpensive replacement for cell culture techniques for diagnosing infections. (musc.edu)

DNA is a long chain of linear polymers containing deoxyribose sugars and their covalently bonded bases known as nucleic acids. (wikibooks.org)

This study addresses the potential for a broad and well-characterized set of control strains relative to virulence factor amplification and confirmed by Southern hybridization. (cdc.gov)
We used PCR amplification and Southern blot hybridization to detect 9 virulence factors among 40 EHEC type-strains from the STEC Center, National Food Safety and Toxicology Center, Michigan State University (East Lansing, MI, USA). (cdc.gov)

Labeling effectiveness can quickly be determined on an agarose gel. (thomassci.com)

The murine CD2 gene was assigned to chromosome 3 by Southern blot analysis of mouse-hamster somatic cell hybrids. (ox.ac.uk)

Manipulating with nucleic acid is a trend in biotechnology. (mcqexams.com)

Formamide is used primarily as a component in probe hybridization solutions to promote probe hybridization specificity. (tainhatvip.net)

However the exact concentration of protease should be empirically determined because excessive digestion can cause complete release of nucleic acids and even cell morphology damage and cell loss. (ice-hbv.org)

Unfolding and folding of the double helix is a common phenomenon in vivo (e.g. in the biosynthesis of nucleic acids). (wikilectures.eu)

After washing, the captured nucleic acid can be released (eluted) with a suitable hydrogen-bonding agent. (wikilectures.eu)

HPV probe that can be optimized for use with polymer detection system or used as optimized RTU with Enzo's Streptavidin detection methods. (enzolifesciences.com)

To identify the mutant ES cell clones to be microinjected, two Southern blots are usually performed: one to detect ES clones in which homologous recombination has occurred, and the other to verify the number of NEO cassettes. (biomedcentral.com)

It bears his name ( Southern blotting ) and is widely used. (wikilectures.eu)
This method was found to be useful, easy, sensitive and fast and could substitute for the widely used, but laborious Southern blot method. (biomedcentral.com)

Fluorescence microscopy of tissue sections from cod injected with fluorescein-labelled pDNA confirmed intracellular uptake of pDNA by the endocardial cells of the atrium and ventricle. (silverchair.com)

Southern blot was performed using standard conditions for gel run, transfer and hybridisation. (biomedcentral.com)

Diseases1

Chemicals and Drugs8

Analytical, Diagnostic and Therapeutic Techniques and Equipment6

Phenomena and Processes3

Information Science2

Health Care1

Hybridization probes3

Electrophoresis14

Situ8

Sequences8

Detection of nucleic1

Oligonucleotide3

Protein16

CDNA4

Proteins8

Polymerase chain re2

Slot blot hybridization5

MRNA2

Vitro3

Membrane2

Nitrocellulose1

Complementary3

Genomic4

Western blot1

Polypeptides2

Fragments1

Analyses1

Assay1

Single stranded1

Antigen2

Synthesis6

Reaction4

Sensitivity1

Mixture4

Molecular probes1

Molecules3

Amino acid seq1

Incubation1

Biotin5

Fragment4

Routinely3

Deoxyribonucleic2

Assays4

Specific2

Covalently1

Amplification2

Agarose gel1

Chromosome1

Biotechnology1

Formamide1

Digestion1

Vivo1

Suitable1

Polymer1

Clones1

Widely2

Tissue1

Hybridisation1