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  • library
  • Using a 2000-member peptide library, we sought to systematically investigate this observation by comparing unlabeled peptides with the two highest-plex isobaric tags (iTRAQ8 and TMT10) across CID, HCD, and ETD fragmentation using high resolution high mass accuracy Orbitrap instrumentation. (nih.gov)
  • done
  • The patented invention provides a lyophilized kit form that enables labeling to be done very rapidly, which is critical for radionuclides having a short half-life. (cnbc.com)
  • 7 - 10 In this paper, labeling was done with 2-nitrobenzenesulfenyl chloride (NBSCl), as described previously, 4 which is categorized in an in vitro labeling reagent. (pubmedcentralcanada.ca)
  • particular
  • In addition, the patent now covers the same labeling with F-19, which can be used for MRI, another major imaging modality for depicting, in particular, musculoskeletal and neurological structures. (cnbc.com)
  • interest
  • To realize comparative analysis, separation still has a pivotal role even if the complexity of the peptide mixture of interest can be greatly diminished by using reagents incorporating stable isotopes. (pubmedcentralcanada.ca)
  • mass
  • systematic separation/spotting followed by mass analysis of the NBS-labeled peptides derived from biological samples is described for the first time. (pubmedcentralcanada.ca)
  • In our differential analysis, we label tryptophan residues with 2-nitrobenzenesulfenyl (NBS) chloride 2 , 3 ( 12 C 6 as light reagent and 13 C 6 as heavy counterpart) 4 in combination with a reversed-phase high-performance liquid chromatography (HPLC), an automatic spotter, and a matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) mass spectrometer. (pubmedcentralcanada.ca)
  • lyophilized kit
  • This new patent, expanding on the 10 already issued in the U.S. covering F-18 labeling, now covers methods for producing a simple lyophilized kit that permits labeling within about 20 minutes, which is critical for F-18 that has a half-life of 110 minutes. (cnbc.com)