• Drug discovery has made use of animal knock-out models that highlight the impact of a protein's absence, particularly in the development of disease, and medicinal chemists have leveraged computational chemistry to generate high affinity compounds against disease-causing proteins. (explained.today)
  • a) Illustration of microtubules in a cell, labeled with fluorophore. (microscopyu.com)
  • Provided here is an in-depth discussion of STORM, and more generally SMLM, with attention to multichannel, three-dimensional, and live cell imaging approaches, and a special focus on the critical steps of fluorophore selection, labeling strategies, and imaging buffer formulation. (microscopyu.com)
  • The FUD and mFUD (control) peptides and their 10 kDa, 20 kDa, and 40 kDa PEG conjugates were labeled with the sulfo-Cy5 fluorophore. (springeropen.com)
  • Isothermal titration calorimetry (ITC) and confocal fluorescence microscopy experiments verified FUD and PEG-FUD fibronectin binding activity preservation following sulfo-Cy5 labeling. (springeropen.com)
  • 4-thio-rU modified RNA oligos have been used as photoaffinity probes in the role of substrate analogs for characterizing the enzyme:substrate complex of tRNA:pseudouridine-5S synthase. (fishersci.com)
  • In the case of nonspecific affinity labels (see Section 9.2) many amino acid residues on the enzyme molecule, and on other protein molecules in the sample, can be covalently modified by the affinity label. (basicmedicalkey.com)
  • Despite the structural modification of the labeled ASOs, in vitro target interaction and in vivo pharmacokinetic behaviors were similar to that of the unlabeled ASOs. (springeropen.com)
  • All three variants of this PEG-FUD displayed preserved binding affinity (K d ) and in vitro FN matrix assembly inhibitory potency of the native FUD peptide [ 11 ]. (springeropen.com)
  • Solution-based approaches involve the use of drug analogs that chemically modify target proteins in solution , tagging them for identification. (explained.today)
  • A novel approach to tritium-labeled antisense oligonucleotides (ASO) was established by conjugating N -succinimidyl propionate, as well as maleimide-derivatives, to the 3′-end of ASOs targeting metastasis-associated lung adenocarcinoma transcript 1 (Malat1) containing amino- or sulfhydryl-linkers. (springeropen.com)
  • We have used 8-azidoadenosine 5'-triphosphate (8-N3 ATP), a photoaffinity analog of ATP, to investigate the ATP-binding sites of intact 18 S dynein. (umassmed.edu)
  • Antibodies can label immature or inactive proteins hence their usefulness to quantify functionally relevant pools of signaling molecules is also limited. (nature.com)
  • The cysteine engineered antibodies may be conjugated with chemotherapeutic drugs, toxins, affinity ligands such as biotin, and detection labels such as fluorophores. (justia.com)
  • Having been an intergral part of Richie Hawtin's Minus label in the early 2000's, he's well known for being a live act and producer-with affinity for analog gear and a sound that crosses between Detroit & Chicago with a new wave influence. (tower.com)
  • CV Sessions' are a combination of the time of the coronavirus and the equipment Sula used in these times, which is analog gear connected and modulated with CV cables (control voltage). (clear-spot.nl)
  • Often, carbon-14 is used to label small molecule drug candidates, since it can be introduced in a metabolically stable position. (springeropen.com)
  • They found specific, extremely high binding affinity of BPA for ERR-γ. (nih.gov)
  • B) Synthesis of spin-labeled nucleic acids as hybridization probes for genome detection by EPR. (uc.edu)
  • We designed and synthesized Monovalent, conformationally Unambiguously Spin-labeled LIgands (MUeSLI). (uni-konstanz.de)
  • They also ran competitive binding assays with BPA analogs and other industrial chemicals, including phenol derivatives, to identify which structural characteristics of the chemicals are critical for binding ERR-γ and maintaining its constitutive activity. (nih.gov)
  • One such approach is to map this site by developing potent and high affinity probes that bind to the receptor covalently, followed by ligand-protein structure characterization. (northeastern.edu)
  • We have designed, synthesized and biochemically evaluated novel electrophilic and photo affinity covalent probes of the CB1 allosteric site. (northeastern.edu)
  • BPA analogs varied in their ability to bind the receptor, and phenol derivatives were newly discovered to be potential candidates for ERR-γ-mediated endocrine disruption. (nih.gov)
  • platform available from BioNano Genomics is able to generate genomic maps through nicking long DNA with Nt.BspQI which recognizes a unique seven base sequence GCTCTTC.5 The nick sites are then filled with a modified dUTP analog with an attached fluorescent probe thereby generating a unique barcode pattern that corresponds to a specific location within the genome of the organism. (academicediting.org)
  • Using tritium-labeled BPA, the researchers conducted the first saturation binding assay to precisely characterize how strongly BPA binds ERR-γ. (nih.gov)
  • Stable isotope labeling allows researchers to study metabolic pathways in vivo in a safe manner. (bocsci.com)
  • JWH122 is a synthetic cannabinoid with high affinity for both CB1 and CB2 receptors. (bocsci.com)
  • Acyclovir is a synthetic nucleoside analogue active against herpesviruses. (nih.gov)
  • Mechanism of Antiviral Action: Acyclovir is a synthetic purine nucleoside analogue with in vitro and in vivo inhibitory activity against herpes simplex virus types 1 (HSV-1), 2 (HSV-2), and varicella-zoster virus (VZV). (nih.gov)
  • Among these ligands, GAT100 emerged to be the most potent, first-in-class analog which labels the receptor covalently. (northeastern.edu)
  • The inhibitory activity of acyclovir is highly selective due to its affinity for the enzyme thymidine kinase (TK) encoded by HSV and VZV. (nih.gov)
  • However, in contrast to what was expected, the activity is significantly decreased compared to its non-fluorinated analog. (uni-konstanz.de)