• 4. The composition of claim 3 , wherein the polymerase comprises Taq DNA Polymerase having a label attached to an amino acid of the Taq DNA polymerase that is less than 60 Å from a nucleotide that would be incorporated by said polymerase. (google.com)
  • The authors, using a known Rev binding site in the human immunodeficiency virus (HIV) Rev response element (RRE), searched for a second oligonucleotide by tethering random heptanucleotides to an octanucleotide that bound to the Rev site, and screening with RNase H to determine the strongest site for secondary binding. (google.com)
  • 1994) 116: 437-442, teach the use of a random oligonucleotide library to select superior oligonucleotides that bind to non-contiguous regions of a target RNA to be tethered together in a TOP. (google.com)
  • We recommend the routine addition of 1 mM DTT to all buffers used for handling, labeling and storage of the SNAP-tag. (neb.com)
  • We report the direct dynamic nuclear polarization (DNP) of 13 C nuclei of a uniformly [ 13 C, 15 N]-labeled, paramagnetic full-length hammerhead ribozyme (HHRz) complex with Mn 2+ where the enhanced polarization is fully provided by the endogenously bound metal ion and no exogenous polarizing agent is added. (springer.com)