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  • vitro
  • Human malignant lymphoma lines were used for in vitro experiments, and folpolyglutamate synthetase (FPGS), which induces polyglutamylation, was knocked down with short-hairpin RNA, and a stable cell line with a low rate of polyglutamylation was established. (biomedcentral.com)
  • In vitro, the relief effect of LV after MTX administration was significantly enhanced after FPGS knockdown in al cell lines, whereas enhancement of FPGS expression by NaBu treatment significantly reduced this relief effect. (biomedcentral.com)
  • Mouse leukemia virus, 'spontaneous' release by mouse embryo cells after longterm in vitro cultivation. (jax.org)
  • Effect of changing horse and fetal calf serum supplements on neo- plastic conversion and chromosomal characteristics of mouse embryo cells in vitro. (jax.org)
  • These analogs are able to radiosensitize a wide variety of tumor cell types in vitro and several have proven clinical efficacy as well. (springer.com)
  • tumors
  • Considering effective therapy, relatively slowly growing tumors possessing a large fraction of "nonproliferating" cells are of substantial concern. (springer.com)
  • Atkin , N. B. , 1970, Cytogenetic studies on human tumors and premalignant lesions: The emergence of aneuploid Cell. (springer.com)
  • The early model systems that were developed included Sarcoma 37 (S37), Sarcoma 180 (S180), Walker 256, and Ehrlich's ascites tumor, all carcinogen-induced tumors in mice. (aacrjournals.org)
  • proteins
  • Gangliosides perform important functions through carbohydrate-specific interactions with proteins, for example, as receptors in cell-cell recognition, which can be exploited by viruses and other pathogens, and also by regulating signaling proteins, such as the epidermal growth factor receptor (EGFR) and the vascular endothelial growth factor receptor (VEGFR), through lateral interaction in the membrane. (frontiersin.org)
  • These proteins were upregulated in the L1210-A cells versus L1210-S, which was verified by Western blot analysis. (metabolomicscentre.nl)
  • mammalian cells
  • These lipids have highly diverse structures, not the least with respect to their carbohydrate chains, with N -acetylneuraminic acid (NeuAc) and N -glycolylneuraminic acid (NeuGc) being the two most common sialic-acid residues in mammalian cells. (frontiersin.org)
  • HeLa
  • Originally, we showed that HeLa and Hep3B carcinoma cells undergo different proliferation responses in hypoxia. (biomedsearch.com)
  • Terasima, T., and Tolmach, L. J. Variations in several responses of HeLa cells to X-irradiation during divison cycle. (springer.com)
  • versus
  • other published examples include vincristine AUC versus severity of neurotoxicity, etoposide systemic exposure versus leukopenia, red cell concentration of mercaptopurine metabolites versus neutropenia in children with ALL, and ARA-CTP retention in leukemic blasts versus clinical response in acute non-lymphocytic leukemia. (curehunter.com)
  • human
  • Different cells and tissues show differential expression of GSLs, and changes in structures of GSL glycan moieties occur during development of numerous types of human cancer. (frontiersin.org)
  • Lee, L.-S., and Cheng, Y. Human deoxythymidine kinase II: substrate specificity and kinetic behavior of the cytoplasmic and mitochondrial isozymes derived from blast cells of acute myelocytic leukemia. (springer.com)
  • Incorporation of 5-bromo-and 5-iododeoxyuridine into the deoxyribonucleic acid of human cells and its effect on radiation sensitivity. (springer.com)
  • BALB
  • Induction of plasma cell tumours in balb/c mice with 2,6,10,14- -tetramethylpentadecane (pristane). (jax.org)
  • polymerase
  • Additional sites which may facilitate the inhibition of cell growth were IMP dehydrogenase, thymidine kinase, TMP kinase and TDP kinase, m-RNA, r-RNA and t-RNA polymerase activities as well as topoisomerase I1 activity. (docme.ru)
  • Keywords: Boron, anti-neoplastic, leukemia, topoisomerase 11, RNA polymerase, DNA polymerases, acute toxicity INTRODUCTION There is rapidly growing interest in the biological activity of boron compounds in animals, including humans. (docme.ru)
  • sarcoma
  • NCI conducted tests of the constituents for antitumor activity in a mouse tumor model (the Sarcoma 37 test), and found that all were highly active in that test system (384). (quackwatch.org)
  • Transplantable osteogenic sarcoma in inbred akr mice. (jax.org)
  • The effects in mice of cortisol combined with thymus antiserum on sarcoma 180 and antibody responses. (jax.org)
  • precursors
  • Using stromal/hematopoietic cell cocultures, we investigated the effects of stroma-derived signals on apoptosis sensitivity in hematopoietic precursors. (biomedsearch.com)
  • The later stages of the present studies were concerned with biotransformation of the various synthetic precursors in both TRP4a whole cell systems and crude enzyme preparations (cell free extract, or CFE). (ubc.ca)
  • proliferation
  • proliferation in the castrate mouse seminal vesicle in response to testosterone propionate. (springer.com)
  • The mechanisms by which cells undergo proliferation arrest or cell death in response to hypoxia are still not completely understood. (biomedsearch.com)
  • Glycogen synthase kinase 3beta (GSK3beta) regulates numerous signaling pathways that control a wide range of cellular processes, including cell proliferation, differentiation, apoptosis and metabolism. (biomedsearch.com)
  • treatment
  • Treatment of RINm5F with resistin induced cell damage. (biomedsearch.com)
  • Direct monitoring of cell death (i.e., apoptosis and necrosis) during or shortly after treatment is desirable in all cancer therapies to determine the outcome. (biomedsearch.com)
  • We hypothesized that the extent of polyglutamylation in tumor cells determines treatment resistance. (biomedcentral.com)
  • Cell viability after MTX treatment with LV rescue was evaluated using sodium butyrate (NaBu), a histone-deacetylase inhibitor that induces polyglutamylation by elevating FPGS expression. (biomedcentral.com)
  • metabolites
  • The initial phases of the research involved isolation and identification of diterpene metabolites from TRP4a cell cultures. (ubc.ca)
  • Cultures
  • This thesis is concerned with the use of plant cell cultures in combination with organic synthesis to provide efficient routes for the synthesis of novel diterpene analogs of triptolide (Tl) and tripdiolide (Td), the two most active principles of the Chinese medicinal plant Tripterygium wilfordii. (ubc.ca)
  • Incubation of isotriptophenolide in TRP4a cell cultures produced two novel epoxy dienones, (7,8)β-epoxy-19-hydroxy-12-oxo-18(4->3)afce0-abieta-3,9(ll),13- trien-18-oic acid lactone, and (7,8)α-epoxy-19-hydroxy-12-oxo-18(4->3)aZ?eo-abieta- 3,9(1 l),13-trien-18-oic acid lactone, along with additional hydroxylated compounds. (ubc.ca)
  • Older cell cultures (21 day) tend to give higher yields of these epoxides. (ubc.ca)
  • Biotransformation of isotriptophenolide with CFE preparations from TRP4a cell cultures gave comparable results as the whole cell system except that the biotransformation proceeded much faster. (ubc.ca)
  • Incubation of triptophenolide in whole cell cultures and CFEs showed only a low level of biotransformation in comparison with isotriptophenolide. (ubc.ca)
  • Biotransformation of demethyl isoneotriptophenolide in whole cell cultures showed rapid consumption of the precursor. (ubc.ca)
  • lymphocytes
  • Dexamethasone (DEX) induces apoptosis in lymphocytes, while protecting some cancer cells from apoptosis, by a poorly understood mechanism. (biomedsearch.com)
  • cancer cells
  • A steadily increasing number of subsequent studies described aberrant expression and function of GSLs and related enzymes in cancer cells and tissues. (frontiersin.org)
  • In laboratory studies, isoflavones slowed down the growth of several different types of cancer cells, including breast and prostate cancers. (mskcc.org)
  • Lu J, Choi E, Tamanoi F, Zink JI (2008) Light-activated nanoimpeller-controlled drug release in cancer cells. (springer.com)
  • synthesis
  • The reduction in enzyme activities led to sufficient reduction of d(NTP) levels to suppress DNA synthesis and cell growth. (docme.ru)