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  • genetically
  • This allows an analysis to be repeated many times on genetically identical cells which is desirable for repeatable scientific experiments. (wikipedia.org)
  • Further, cell lines can change genetically over multiple passages, leading to phenotypic differences among isolates and potentially different experimental results depending on when and with what strain isolate an experiment is conducted. (wikipedia.org)
  • These COS cells are genetically modified to produce the T antigen from their own genome. (wikipedia.org)
  • viable
  • Also, if your freeze killed a lot of the cells, the viable cell plating density after thawing is even lower. (bio.net)
  • But even scientists from the named institutes have raised doubts that all their lines are viable. (newscientist.com)
  • However, during this period of research, the team also discovered that if cells were properly stored in a freezer, cells would remain viable and could provide enormous numbers of cells for research purposes. (wikipedia.org)
  • indefinitely
  • An immortalized cell line is a population of cells from a multicellular organism which would normally not proliferate indefinitely but, due to mutation, have evaded normal cellular senescence and instead can keep undergoing division. (wikipedia.org)
  • An immortalized cell line should not be confused with stem cells, which can also divide indefinitely, but form a normal part of the development of a multicellular organism. (wikipedia.org)
  • But not all of the lines included on the NIH list have been shown to be able to divide indefinitely, or to be pluripotent - that is, able to develop into many different tissues. (newscientist.com)
  • cancer
  • There are several methods for generating immortalized cell lines: Isolation from a naturally occurring cancer. (wikipedia.org)
  • Artificial expression of key proteins required for immortality, for example telomerase which prevents degradation of chromosome ends during DNA replication in eukaryotes Hybridoma technology, specifically used for the generation of immortalized antibody-producing B cell lines, where an antibody-producing B cell is fused with a myeloma (B cell cancer) cell. (wikipedia.org)
  • As a bi-institutional facility the Antibody and Bioresource Core Facility distributes cell lines developed at Memorial Sloan Kettering Cancer Center and The Rockefeller University. (rockefeller.edu)
  • Clinically relevant cell models are critical for studies of molecular and cellular mechanisms of tumors, as well as for drug screening for cancer. (atcc.org)
  • Cancer Cell. (wikipedia.org)
  • Unlike the other commonly utilized, Androgen receptor positive prostate cancer cell lines LNCaP and VCaP, LAPC4 have high expression of Keratin 5, a basal marker, as well as the luminal markers Keratin 8 and Keratin 18. (wikipedia.org)
  • These bundles become the nuclei of the daughter cells, Out-of-control cell replication is the underlying cause of cancer, developing drugs that interfere with microtubule activity offers one potential therapeutic approach. (wikipedia.org)
  • investigators
  • NIH notice NOT-OD-15-103, Enhancing Reproducibility through Rigor and Transparency, includes provisions recommending demonstration of cell-line authentication from all investigators applying for NIH grants. (genengnews.com)
  • Details such as how well the cell lines divide and the molecular markers of their pluripotency will be provided - but only at the discretion of the investigators who derived the lines. (newscientist.com)
  • receptors
  • The hybridoma D0-11.10, a mouse monoclonal antibody against receptors for antigen plus I region products on T cells. (nationaljewish.org)
  • Hybridomas expressing various combinations of gamma delta T cell receptors available as research tools. (nationaljewish.org)