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  • intracellular
  • In contrast, the role of intracellular trafficking mechanisms in controlling BK channel function, especially in live cells, has been less studied. (frontiersin.org)
  • Subunit
  • Modulation of BK channel gating is well characterized, regulated by accessory subunit interactions, intracellular signaling pathways, and membrane potential. (frontiersin.org)
  • Utilizing recent improvements in the signal-to-noise ratio of voltage-sensitive dye imaging in mouse brain slices, we demonstrate that intracortical axon collaterals and en passant presynaptic terminals of layer 5 pyramidal cells exhibit a high density of Kv1 subunit-containing ion channels, which generate a slowly inactivating K + current critically important for spike repolarization in these compartments. (jneurosci.org)
  • phosphorylation
  • The uncovered novel effects of m1 receptor activation include ligand-independent EGF receptor activation, Pyk2 tyrosine kinase stimulation, Kv1.2 channel regulation via physical association with the small GTPase RhoA, and tyrosine phosphorylation-dependent association between the channel and receptor tyrosine phosphatase which in turn terminates channel suppression by m1 receptor. (searlescholars.net)
  • We explored whether a specific interaction between PSD95 and K V 1 channels enables protein kinase A phosphorylation of K V 1 channels in cVSMCs to promote vasodilation. (ahajournals.org)
  • These findings provide initial evidence that protein kinase A phosphorylation of K V 1 channels is enabled by a dynamic association with PSD95 in cerebral arteries and suggest that a disruption of such association may compromise cerebral vasodilation and blood flow. (ahajournals.org)
  • blocker
  • Patch-clamp recordings confirmed that K V 1-C peptide attenuates K V 1 channel blocker (5-(4-phenylalkoxypsoralen))-sensitive current in cVSMCs. (ahajournals.org)
  • Accordingly, treating the stimulated tissue with a potassium channel blocker, 4-aminopyridine, led to the appearance of a shoulder peak in the compound action potential curve. (purdue.edu)
  • tyrosine
  • The unexpected finding of tyrosine kinase-mediated Kv1.2 potassium channel suppression by m1 receptor in neuroblastoma-glioma cells as well as expression systems received wide attention, pointing to connections between signaling pathways previously thought to be separate. (searlescholars.net)
  • complexes
  • Recently, we reported the expression of a scaffolding protein, postsynaptic density protein-95 (PSD95), in rat CA. 16 Previously, PSD95 was studied primarily in neurons, where it provides an assembly platform at the plasma membrane for macromolecular signaling complexes including ion channels. (ahajournals.org)
  • ionic channels
  • The computation performed by a single cortical neuron depends upon the distribution and properties of ionic channels in its axon collaterals, since these determine the shape of action potentials in local presynaptic terminals, the rate and amplitude of calcium influx, and subsequently the probability of neurotransmitter release. (jneurosci.org)
  • modulation
  • In addition to the truly original and valuable leads that these studies have provided to the signaling field, the specific findings on m1 modulation of Kv1.2 channels will have major impact on our understanding of central nervous system functions. (searlescholars.net)
  • tissues
  • This novel mouse line provides a versatile fluorescent platform for studying endogenous BK channels in living and fixed tissues. (frontiersin.org)
  • presynaptic
  • These results indicate that action potential-induced synaptic transmission may operate through a mix of analog-digital transmission owing to the properties of Kv1 channels in axon collaterals and presynaptic boutons. (jneurosci.org)
  • aberrant
  • Here we show that regulated and aberrant glycosylation modulate cardiac ion channel activity and electrical signaling through a cell-specific mechanism. (pnas.org)
  • isoform
  • Glycans attached to ion channels alter channel activity through isoform-specific mechanisms. (pnas.org)
  • A recent work showed that variable sialic acid levels attached to a single Na v isoform, Na v 1.5, were responsible for differences in channel gating observed among neonatal and adult atrial and ventricular cardiomyocytes ( 15 ). (pnas.org)
  • produces
  • A point mutation of Lys23 to alanine in maurotoxin produces a 1000-fold reduction in the IC50 of block by the toxin suggesting the importance of this charged residue for the interaction with the channel. (smartox-biotech.com)
  • mechanism
  • Though seemingly "solved" long ago, in recent years this issue has resurfaced in discussions of the voltage-gating mechanism of K + channels, and the likely configuration of charged arginine side chain of the voltage sensor with respect to the membrane lipids ( Mackinnon, 2005 ). (rupress.org)