In this study, we examined the role of PPARα in sleep/wake regulation using PPARα knockout (KO) mice. (tokushima-u.ac.jp)
The initiative is projected to take 10 years (until 2021), and will focus on analysing homozygous mutant mice generated on an isogenic C57BL/6N background by the International Knockout Mouse Consortium. (wikipedia.org)
Around 30% of all targeted gene knockouts in mice result in embryonic or perinatal death. (wikipedia.org)
Mice are currently the laboratory animal species most closely related to humans for which the knockout technique can easily be applied. (wikipedia.org)
Aspects of the technology for generating knockout mice, and the mice themselves have been patented in many countries by private companies. (wikipedia.org)
Consequently, observing the characteristics of knockout mice gives researchers information that can be used to better understand how a similar gene may cause or contribute to disease in humans. (wikipedia.org)
Examples of research in which knockout mice have been useful include studying and modeling different kinds of cancer , obesity , heart disease , diabetes , arthritis , substance abuse , anxiety , aging and Parkinson's disease . (wikipedia.org)
Knockout mice also offer a biological and scientific context in which drugs and other therapies can be developed and tested. (wikipedia.org)
Millions of knockout mice are used in experiments each year. (wikipedia.org)
There are several thousand different strains of knockout mice. (wikipedia.org)
Breeding scheme for producing knockout mice. (wikipedia.org)
Conditional knockout (cKO) mice can be used to study specific gene functions and related human diseases. (cyagen.com)
Compared with traditional knockout (KO) mice, conditional knockout mice are obtained with a gene that deleted only in specific tissues or on an inducible basis. (cyagen.com)
Conditional knockout (cKO) mice are genetically engineered animal models in which certain gene is inactivated in a specific tissue or on an inducible basis. (cyagen.com)
What Applications are Conditional Knockout Mice Used For? (cyagen.com)
Conditional knockout (cKO) mice are crucial tools for revealing gene functions, gaining proper understanding of human disease mechanisms, and verifying compound specificity. (cyagen.com)
How Are Conditional Knockout Mice Generated? (cyagen.com)
If you are planning generating conditional knockout mice, Cyagen's experts can provide you with in-depth technical support and discuss your project plans. (cyagen.com)
Here, we report that hepatocyte-specific Fitm2 knockout (FIT2-LKO) mice fed a chow diet exhibited elevated acyl-CoA levels, ER stress, and signs of liver injury. (sdsc.edu)
In Atm-knockout (Atm-KO) mice, complete loss of Atm leads to a very mild neurological phenotype, suggesting that Atm loss is not sufficient to markedly abrogate cerebellar structure and function in this organism. (eur.nl)
Objective: The aim of this study was to determine the effect of high salt intake on the level of inflammation markers and to determine whether there is a difference between the wild type and the Tff3-/- knockout mice. (mefos.hr)
Conditional9
In many cases, strains carrying one of two types of alleles will be archived - a null allele used in the primary IMPC phenotyping pipeline and a conditional ready allele that allows tissue restricted knockouts via the Cre-Lox Recombination and FLP-FRT recombination systems. (wikipedia.org)
The North American Conditional Mouse Mutagenesis project (NorCOMM) is a large-scale research initiative focused on developing and distributing a library of mouse embryonic stem (ES) cell lines carrying single conditional knockout mutations across the mouse genome. (tigm.org)
In order to determine the physiological function of GSKIP, in particular its involvement in PKA and GSK3β signalling, a conditional knockout (KO) mouse model had been developed. (fu-berlin.de)
Conditional knockout (cKO) mouse models also allow precise control of gene expression for a functional protein target, providing an in vivo system to evaluate potential off-target effects of a drug. (cyagen.com)
Take, for example, Raly conditional knockout mouse - the liver-specific deletion of hnRNP Raly presents lower serum cholesterol and hepatic lipid content in the described mouse model. (cyagen.com)
Conditional knockout (cKO) models are often referred to as 'floxed' models due to the use of Cre/lox system in their development. (cyagen.com)
The Cre-Lox recombination system enables the generation of tissue-specific or inducible knockouts with a high level of control over the spatial and temporal expression of genes, and greatly improves the efficiency and specificity in generating conditional mouse. (cyagen.com)
2011) A conditional knockout resource for the genome-wide study of mouse gene function. (mmrrc.org)
To circumvent this issue, a novel conditional mouse knockout model was established. (bl.uk)
Popular GitHub repositories1
This package is not used by any popular GitHub repositories. (nuget.org)
International Knockout Mouse Consortium1
The members of the International Knockout Mouse Consortium are working together to mutate all protein-coding genes in the mouse using a combination of gene trapping and gene targeting in C57BL/6 mouse embryonic stem (ES) cells. (tigm.org)
Public Repositories1
During June 2021-January 2022, CDC expanded genomic surveillance data sources to incorporate sequence data from public repositories to produce weighted estimates of variant proportions at the jurisdiction level and refined analytic methods to enhance the timeliness and accuracy of national and regional variant proportion estimates. (cdc.gov)
Gene knockout4
Gene knockout in rats is much harder and has only been possible since 2003. (wikipedia.org)
Using this technique, the appearance of several S. aureus gene knockout strains were studied. (eur.nl)
We demonstrated that in an S. aureus srtA gene knockout strain, srtA-dependent cell-surface-associated proteins were detectable yet not covalently linked to the PG layer. (eur.nl)
To provide the best chance of creating a functional gene knockout, CompoZr Knockout ZFNs are targeted to the first 2/3 of the coding region for the gene of interest. (calcimimeticagent.com)
Https1
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001476. (sdsc.edu)
Strains2
The International Mouse Phenotyping Consortium (IMPC) is an international scientific endeavour to create and characterize the phenotype of 20,000 knockout mouse strains. (wikipedia.org)
Mouse strains generated by the IMPC partners are deposited at the KOMP repository and the European Mutant Mouse Archive. (wikipedia.org)
Mutants2
In 2013 the IMPC published the Bloomsbury report on mouse embryo phenotyping, outlining a standard pipeline for the screening of embryonic-lethal knockouts in homozygote mutants. (wikipedia.org)
The double knockout (DKO) apyrase pollen displayed a complete block of pollen germination, which implicated this step as the cause of the lethality of apyrase double knockout mutants. (utexas.edu)
Proteins3
Gene-expression analysis of Sin1 knockout cortical neurons demonstrated an important down-regulation of transcription factors, cytoskeletal proteins and components of signalling pathways involved in neuronal survival, aiding to uncover the mechanism by which Sin1 promotes neuronal survival. (bl.uk)
As of August 2023, they have presented comprehensive knockout characterisation data for 812 antibodies and 78 proteins using techniques such as Western blot, immunoprecipitation, and immunofluorescence. (bvsalud.org)
Focusing on antibodies for human proteins, we have scaled a standardized characterization approach using parental and knockout cell lines (Laflamme et al. (bvsalud.org)
Genes2
However, systematic studies of embryonic-lethal knockouts are important to understand how these genes influence embryo development and survival. (wikipedia.org)
H3.3 knockout followed by RNA-seq reveals large-scale transcriptional alterations in functionally important genes. (biomedcentral.com)
Login1
Please login to the repository to save this object to your list. (mefos.hr)
Physiology2
They are widely used in knockout experiments, especially those investigating genetic questions that relate to human physiology . (wikipedia.org)
The first recorded knockout mouse was created by Mario R. Capecchi , Martin Evans , and Oliver Smithies in 1989, for which they were awarded the 2007 Nobel Prize in Physiology or Medicine . (wikipedia.org)
Genetically1
A knockout mouse , or knock-out mouse , is a genetically modified mouse ( Mus musculus ) in which researchers have inactivated, or " knocked out ", an existing gene by replacing it or disrupting it with an artificial piece of DNA . (wikipedia.org)
Strain1
IMPReSS was launched in 2011 to help the IMPC achieve its goal of characterizing a knockout mouse strain for every gene and will continue to be actively developed for the ten year life-time of the project. (wikipedia.org)
Functional1
Through the cellular process of Non-Homologous End Joining, this double strand break can result in modification of the DNA sequence and therefore create a functional knockout of the targeted gene. (calcimimeticagent.com)
JavaScript1
The mapping plugin gives you a straightforward way to map plain JavaScript objects into a view model with the appropriate Knockout observables. (nuget.org)
Data5
refine.bio is a repository of uniformly processed and normalized, ready-to-use transcriptome data from publicly available sources. (refine.bio)
However, this is not possible because we are using `data-bind="text: error"` which means that Knockout.js will use the `.error` attribute to fill the content of this tag dynamically. (readthedocs.org)
Data was gathered from the Brazilian Government open data repository ( dados.gov.br ) and analyzed with the help of MySql. (thoughtworks.com)
The neutron knockout reaction and inelastic proton scattering were applied for 61V while the neutron knock-out reaction provided the data for 59V. (uni-koeln.de)
YCharOS consolidates its data into reports (one protein per report) available on Zenodo, a public repository controlled by CERN, to ensure open access. (bvsalud.org)
Form1
Physical form: CompoZr Knockout ZFNs are provided as 10 aliquots of mRNA encoding for the ZFNs along with plasmid DNA for both ZFN pairs. (calcimimeticagent.com)
Model2
Altered mGluR5-Homer scaffolds and corticostriatal connectivity in a Shank3 complete knockout model of autism. (duke.edu)
Here, we describe a complete knockout mouse model of the autism-associated Shank3 gene, with a deletion of exons 4-22 (Δe4-22). (duke.edu)
Navigation1
Navigation & Screen State Management -Consistent Async Programming w/ Promises -App Bundling and Optimization -Use any Backend Technology -Built on top of jQuery, Knockout & RequireJS. (nuget.org)
Cells2
Blastocysts containing cells, that are both wildtype and knockout cells, are injected into the uterus of a foster mother. (wikipedia.org)
RNA Sanger sequencing analysis was performed in AEG‑1‑deficient HT22 cells and wild‑type HT22 cells without knockout (KO). (spandidos-publications.com)
Provide1
Please provide Taconic Biosciences with your contact information so we can promptly respond to your Knockout Repository inquiry. (taconic.com)
Website1
The website is a single page, responsive, client side app using mainly knockout.js and require.js. (thoughtworks.com)
Double1
General description: CompoZr Knockout ZFNs create targeted double strand breaks at a gene specific target locus. (calcimimeticagent.com)
Control1
Also included in the kit are custom-designed primers, that allow for determination of the rate of mutation and for screening of individual clones that have the desired mutational event, and positive control DNA that has been modified using the CompoZr Knockout ZFN. (calcimimeticagent.com)