Knockout mouse phenotyping. Medical search. Frequent questions

The International Mouse Phenotyping Consortium (IMPC) is an international scientific endeavour to create and characterize the phenotype of 20,000 knockout mouse strains. (wikipedia.org)
The initiative is projected to take 10 years (until 2021), and will focus on analysing homozygous mutant mice generated on an isogenic C57BL/6N background by the International Knockout Mouse Consortium. (wikipedia.org)
We have also integrated global recommendations of the International Mouse Phenotyping Consortium (IMPC) , which identified important parameters that will collectively summarize the consequences of removing the function of a single gene in the context of the whole animal. (jax.org)
The International Mouse Phenotyping Consortium (IMPC) is aiming to produce a complete catalogue of mammalian gene function across all genes. (sciencedaily.com)
The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data. (nih.gov)
The International Mouse Phenotyping Consortium project is systematically phenotyping knockout mice from the mutant ES cells produced by the International Mouse Knockout Consortium. (jax.org)
Using mice, the International Mouse Phenotyping Consortium (IMPC) aims to target each protein-coding gene and phenotype multiple organ systems in single-gene knockout models by a global network of mouse clinics. (springer.com)
The International Mouse Phenotyping Consortium, IMPC, has set the goal of systematically "knocking out" each protein-coding genes in the mouse and phenotyping these so-called knockout (KO) mice using highly standardized high-throughput tests (Brown and Moore 2012 ). (springer.com)
The IMPC consortium uses different targeting strategies that have different and complementary properties to produce knockout alleles. (mousephenotype.org)
Over 4700 lines generated from the International Knockout Mouse Consortium (IKMC) ES cell resources e.g. (infrafrontier.eu)
EUCOMM mice, and International Mouse Phenotyping Consortium (IMPC) mutant resources. (infrafrontier.eu)
Many genetically altered mice are already available "off the shelf" as part of the International Knockout Mouse/Mouse Phenotyping Consortium . (emoryhealthsciblog.com)
AMMRA can be missioned to world connection by working carefully with the consortiums such because the Worldwide Mouse Phenotyping Consortium and the Worldwide Mouse Pressure Useful resource . (eumorphia.org)
As part of the International Mouse Phenotyping Consortium, we undertook a hearing loss screen in a cohort of 3006 mouse knockout strains. (lih.lu)
2013). A unique but related substrain closely, C57BL/6NJ, may be the strain useful for all knockout mice generated beneath the International Knockout Mouse Consortium (Skarnes et al. (bassresearch.com)
2011) and seen as a the International Mouse Phenotyping Consortium (Koscielny et al. (bassresearch.com)

Mouse strains generated by the IMPC partners are deposited at the KOMP repository and the European Mutant Mouse Archive. (wikipedia.org)
In many cases, strains carrying one of two types of alleles will be archived - a null allele used in the primary IMPC phenotyping pipeline and a conditional ready allele that allows tissue restricted knockouts via the Cre-Lox Recombination and FLP-FRT recombination systems. (wikipedia.org)
IMPReSS was launched in 2011 to help the IMPC achieve its goal of characterizing a knockout mouse strain for every gene and will continue to be actively developed for the ten year life-time of the project. (wikipedia.org)
In 2013 the IMPC published the Bloomsbury report on mouse embryo phenotyping, outlining a standard pipeline for the screening of embryonic-lethal knockouts in homozygote mutants. (wikipedia.org)
A team led by researchers at IMPC member Institute UC Davis have used IMPC data to identify novel genes associated with oculocutaneous syndromes in gene targeted 'knockout' mice, in research that is potentially relevant to human conditions such as albinism and phakomatoses. (mousephenotype.org)
The IMPC is systematically 'knocking out' or removing every gene in the mouse genome, (around 20,000 genes) one at a time, and examining the effects that this has on the mouse's biological systems. (mousephenotype.org)
This is why the IMPC is aiming to build a complete database that systematically details the functions of all areas of the mouse genome, including neurological, metabolic, cardiovascular, respiratory and immunological systems. (sciencedaily.com)
Terry Meehan, IMPC Project Coordinator at European Bioinformatics Institute (EMBL-EBI) said: "Mouse models allow us to speed up patient diagnosis and develop new therapies. (sciencedaily.com)
The IMPC applies a panel of phenotyping screens to characterise single-gene knockout mice by comparison to wild types. (mousephenotype.org)
The analysis uses data from IMPC, along with published data on other mouse mutants, in comparison to human disease reports in OMIM, Orphanet, and DECIPHER. (mousephenotype.org)
The table below lists publications which used either products generated by the IMPC or data produced by the phenotyping efforts of the IMPC. (mousephenotype.org)
No publications found that use IMPC mice or data for Tfe3. (mousephenotype.org)
The IMPC is hunting unknown genes responsible for hearing loss by screening knockout mice. (mousephenotype.org)
In this review, we summarize the current advances of the IMPC in cardiac research and describe in detail the diagnostic requirements of high-throughput electrocardiography and transthoracic echocardiography capable of detecting cardiac arrhythmias and cardiomyopathies in mice. (springer.com)
These extensive high-throughput data from IMPC mice provide a promising opportunity to explore genetics causing metabolic heart disease with an important translational approach. (springer.com)
The IMPC has been increasingly using CRISPR/Cas9 to knockout genes for several years now. (mousephenotype.org)
Here Lauryl Nutter talks about CRISPR/Cas9, its uses for studying mouse genetics and how the technique is being used by the IMPC. (mousephenotype.org)

IMPReSS, the successor of EMPReSS, is built on the concept of a "phenotype pipeline": a sequence of individual procedures performed on a mouse at a specified age and organized to minimize interference from one procedure to the next. (wikipedia.org)
Effective high-throughput mouse phenotyping is therefore critically important to establish baseline genotype/phenotype understanding for functional analysis of the KOMP resource. (jax.org)
Phenotype comparisons summarize the similarity of mouse phenotypes with human disease phenotypes. (mousephenotype.org)
Practical application of ontologies to annotate and analyse large scale raw mouse phenotype data. (uni-trier.de)
Right here, we report the phenotype of mice bred to hold two copies of the BAC, which extra utterly recapitulate the phenotypic options of HGPS in pores and skin, adipose, skeletal, and vascular tissues. (eumorphia.org)

However, systematic studies of embryonic-lethal knockouts are important to understand how these genes influence embryo development and survival. (wikipedia.org)
The analysis of genetic variants in mice has provided profound insights into the function of human genes. (mousephenotype.org)
The research, which generated over 20 million pieces of data, has found 360 new disease models and provides 28,406 new descriptions of the genes' effects on mouse biology and disease. (sciencedaily.com)
Their initial results, now published in Nature Genetics , is based on an analysis of the first 3,328 genes (15 per cent of the mouse genome coding for proteins). (sciencedaily.com)
More than half of the 3,328 genes characterised have never been investigated in a mouse before, and for 1,092 genes, no molecular function or biological process were previously known from direct experimental evidence. (sciencedaily.com)
Over 60 genes are known to cause CH, many of which were first discovered in mice. (sc.edu)
Now based at the Baylor College of Medicine, Monica's research exploits the remarkable conservation in genes and whole chromosome regions between the mouse and human. (biologists.com)
Beyond that, we are linking metabolism to the heart and describing phenotypes that emerge in a set of known genes, when knocked out in mice, such as the leptin receptor ( Lepr ), leptin ( Lep ), and Bardet-Biedl syndrome 5 ( Bbs5 ). (springer.com)
Humans share many genes with mice. (wikipedia.org)
Access to over 1700 mouse strains carring mutations in genes that are implicated in causing more than 1300 distinct rare diseases. (infrafrontier.eu)
As t his review explains , it's possible to introduce mutations into three genes at once and get mice quickly, rather than make each one separately and then breed the mice together, which can take many months. (emoryhealthsciblog.com)
Our mutant screen in neonate mice for genes critical to protective respiratory reflexes is fully funded (#R01HL161142). (molecularneurobiology.org)
Over 100 non-syndromic hearing loss genes have been identified in mouse and human, but we remain ignorant of the full extent of the genetic landscape involved in auditory dysfunction. (lih.lu)
Six genes with known coding area distinctions between C57BL/6J and C57BL/6NJ strains display increased transcript amounts in influenza virus-infected mouse lungs, recommending potential efforts to legislation of disease susceptibility. (bassresearch.com)
El objetivo de nuestro estudio tuvo como objetivo revisar y correlacionar genes involucrados en TEA y aquellos relacionados con la comunicación ultrasónica en estudios con modelos animales de comportamiento social basado en el lenguaje en la base de datos PubMed. (bvsalud.org)

The first results from a functional genetic catalogue of the laboratory mouse has been shared with the biomedical research community, revealing new insights into a range of rare diseases and the possibility of accelerating development of new treatments and precision medicine. (sciencedaily.com)
With its similarity to human biology and ease of genetic modification, the laboratory mouse is arguably the preferred model organism for studying human genetic disease. (sciencedaily.com)
[ 9 ] Interrupted aortic arch is the first cardiovascular pattern formation anomaly to be demonstrated to have a genetic basis in both mouse and human. (medscape.com)
The IDG program has developed molecular probes (small molecules), molecular tools and assays, transgenic mice (specialized genetic lines of laboratory mice), recombinant cell lines, data, and digital resources for 100+ understudied GPCRs, ion channels and kinases. (nih.gov)
Their project will focus on conducting a detailed phenotyping of existing embryonic lethal knockout mice with known genetic defects that result in hypothalamic and/or pituitary malformations. (sc.edu)
Causal impact of a full-gene deletion will be investigated in all organ systems by comparing phenotypic characteristics of KO-mice with wild-type control mice of the same genetic C57BL/6N background (Munoz-Fuentes et al. (springer.com)
They are widely used in knockout experiments, especially those investigating genetic questions that relate to human physiology . (wikipedia.org)
With CRISPR/Cas9, it's possible to inject pieces of RNA that target the desired genetic changes, straight into a one-cell stage mouse embryo. (emoryhealthsciblog.com)
Our intersectional genetic mouse engineering paper is finally out at BMC biology! (molecularneurobiology.org)
In our lab, Jarrett will be involved in colony maintenance, starting up the breeding and handling for our upcoming SIDS genetic screen, and mouse phenotyping. (molecularneurobiology.org)
We additional present that genetic discount of the mechanistic goal of rapamycin (mTOR) considerably extends lifespan in these mice, offering a rationale for pharmacologic inhibition of the mTOR pathway within the therapy of HGPS. (eumorphia.org)
We now have been utilizing the Inbred Lengthy- and Quick-Sleep mouse strains (ILS, ISS) and a recombinant inbred panel derived from them, the LXS, to research the genetic underpinnings of acute ethanol tolerance which is taken into account to be a threat issue for alcohol use issues (AUDs). (eumorphia.org)
C57BL/6J mice have been used extensively in mapping sponsor genetic susceptibility to influenza viruses, typically like a founding component of the BXD genetic reference panel [which descends from C57BL/6J and DBA2/J mouse strains [Boon et al. (bassresearch.com)

The JAX KOMP2 pipeline design is based upon previous large-scale, high-throughput phenotyping programs carried out at The Jackson Laboratory, selecting from them the most successful and high-throughput procedures. (jax.org)
Dr. Battey and Dr. Anderson gave the ICDs an overview of the Knockout Mouse Project (KOMP) and the Knockout Mouse Production and Phenotyping (KOMP2) effort. (nih.gov)

The Genome of C57BL/6J "Eve", the Mother of the Laboratory Mouse Genome Reference Strain. (jax.org)
Mechanistically, influenza trojan an infection in C57BL/6J mice leads to earlier display of edema, elevated immune system cell infiltration, higher degrees of inflammatory cytokines, better injury, and postponed activation of regenerative procedures in contaminated lung tissues in comparison to C57BL/6NJ mice. (bassresearch.com)
This function uncovers a previously unappreciated difference in disease susceptibility between your carefully related C57BL/6NJ and C57BL/6J mice, which might be exploited in potential studies to recognize web host factors and/or particular hereditary components that regulate host-dependent inflammatory systems involved with influenza disease pathogenicity. (bassresearch.com)
2017). The usage of C57BL/6NJ mice as an influenza trojan disease model is normally rarely reported, which is not yet determined whether C57BL/6NJ and C57BL/6J differ within their susceptibility to influenza trojan disease. (bassresearch.com)
We reasoned that certain or more from the hereditary variations that differentiate C57BL/6J and C57BL/6NJ mice may regulate influenza trojan disease susceptibility, and when so, such details could be not just needed for influenza research workers to create appropriate tests with knockout mice, but additionally, yet another system by which book hereditary regulators of influenza trojan disease susceptibility may be discovered. (bassresearch.com)

The project involves going through the mouse genome systematically and knocking out a particular gene, one by one, in different mice. (sciencedaily.com)

The 'knockout-first' allele design is a flexible strategy that can be used to produce reporter knockouts, conditional knockouts, and null alleles following exposure to site-specific recombinases Cre and Flp . (mousephenotype.org)
Technicians there can generate a dazzling array of conditional knockouts. (emoryhealthsciblog.com)

Many of these programs generated rich public data sets containing a variety of multi-dimensional molecular and phenotypic data from several organisms including mouse and human. (nih.gov)
The Wellcome Trust funded the acquisition of a limited number of gene knockout mouse strains and associated phenotypic data from Deltagen Inc. and Lexicon Pharmaceuticals. (infrafrontier.eu)

The extensive library of single-gene knockout strains generated through the KOMP program will become significantly more valuable with comprehensive assessments of the functional consequences of removing one gene. (jax.org)
The Jackson Laboratory KOMP Phenotyping Center provides a powerful and efficient phenotyping pipeline to assess the physiological, behavioral and morphological effects of eliminating a single gene. (jax.org)
The 'one by one' knockout approach lends itself to rare gene discovery, as often these diseases are caused by variants of a single gene. (sciencedaily.com)

The aversion of mice to water has been exploited in the design of various behavioural tests, including the forced swim test, the water T-maze test, and the Morris water maze test [ 2 ]. (degruyter.com)
Unsupervised detection of mouse behavioural anomalies using two-stream convolutional autoencoders. (uni-trier.de)
The fragile X knockout (FX KO) mouse also shows a loss of FMRP, as well as many of the physical and behavioural characteristics of human FXS. (ox.ac.uk)

The Mouse Transgenic and Gene Targeting Core (TMF) had a solution: cryopreservation. (emoryhealthsciblog.com)
Just a follow-up to last week's announcement from the Emory Transgenic Mouse and Gene Targeting core that they are offering CRISPR/Cas9 gene editing for mice. (emoryhealthsciblog.com)

Transcriptome analysis of mouse stem cells and early embryos. (nih.gov)
The European Mutant Mouse Archive - EMMA comprises over 8700 mutant lines that are cryopreserved as frozen sperm and/or embryos. (infrafrontier.eu)
Background staining occurs in wild type mice and embryos at an incidental rate. (mousephenotype.org)

Phenotyping is currently not planned for a knockout strain of this gene. (mousephenotype.org)

Voznesenskaya, A. (2014) Macronutrient selection by seven inbred mouse strains and three taste-related knockout strains. (monell.org)

The International Mouse Phenotyping Resource of Standardised Screens (IMPReSS) coordinates and presents standardized protocols that are used by mouse research clinics to assess biological characteristics of mutant mouse strains. (wikipedia.org)
The European Mouse Phenotyping Resource for Standardized Screens (EMPReSS), the predecessor for IMPReSS, developed more than a 150 standardized protocols for the characterization of mutant mouse strains across European research institutes as part of the EUMODIC and EUMORPHIA projects. (wikipedia.org)
INFRAFRONTIER ® and European Mouse Mutant Archive - EMMA ® are registered trademarks at the European Union Intellectual Property Office (EUIPO). (infrafrontier.eu)

Mice homozygous for a null allele exhibit neonatal lethality and chondrodystrophy associated with impaired chondroitin sulfate biosynthesis. (jax.org)
Growth retardation and increased apoptosis in mice with homozygous disruption of the Akt1 gene. (nature.com)
White heterozygous mice can subsequently be crossed to produce mice that are homozygous for the knocked out gene. (wikipedia.org)

Flp converts the 'knockout-first' allele to a conditional allele (tm1c), restoring gene activity. (mousephenotype.org)

Despite their many visual differences, mice are genetically very similar to humans. (mousephenotype.org)
A knockout mouse , or knock-out mouse , is a genetically modified mouse ( Mus musculus ) in which researchers have inactivated, or " knocked out ", an existing gene by replacing it or disrupting it with an artificial piece of DNA . (wikipedia.org)
They allow to visualize and concomitantly manipulate genetically defined cells in mice with single-cell resolution. (infrafrontier.eu)
Cryopreservation is a reliable and efficient method for archiving and distributing genetically engineered mouse lines," says Karolina Piotrowska-Nitsche, PhD, director of the Core. (emoryhealthsciblog.com)

At KOMP he was involved in the high throughput production of mouse mutants for phenotyping as well as different phenotyping assays. (molecularneurobiology.org)

These knock-out techniques have been well established in mice, but proved to be difficult to accomplish in rats. (bmedia.nl)
Gene knockout in rats is much harder and has only been possible since 2003. (wikipedia.org)
For investigators that use laboratory mice or rats in their research, this posed a significant challenge. (emoryhealthsciblog.com)

Data parameters are associated with biomedical ontology terms in order to facilitate data sharing and to aid in the identification of phenotypic mouse-models of human diseases. (wikipedia.org)
Description: RNA profiling data sets generated by the Mouse ENCODE project. (nih.gov)
EuroPhenome: a repository for high-throughput mouse phenotyping data. (uni-trier.de)

The effects of these mutations cannot therefore be studied in live adult mice, except as heterozygote mutants. (wikipedia.org)
The lab recently submitted an RO1 grant with the aim of utilizing a recently developed automated closed loop neonate respiratory assessment platform to screen mouse mutants from the BCM KOMP effort. (molecularneurobiology.org)

A conditional knockout mouse line called Il23r tm2a(EUCOMM)Wtsi was generated at the Wellcome Trust Sanger Institute . (wikidoc.org)

Behavioral phenotyping of neuropeptide S receptor knockout mice. (nature.com)
On the behavioral stage, solely restricted and gentle alterations have been reported, with a big a part of analyzed behaviors being unaffected in GAMT KO as in contrast with wild-type mice. (eumorphia.org)

The mouse strains are characterized in a broad based phenotyping pipeline that is focused on revealing insights into human disease by measuring embryonic, neuromuscular, sensory, cardiovascular, metabolic, respiratory, haematological, and neurological parameters. (wikipedia.org)
This study reports the metabolic phenotyping of a global Abcc5 knockout mouse. (ox.ac.uk)

Subsequently, she was a postdoctoral fellow in the Mammalian Genetics Laboratory at the National Cancer Institute (NCI), the laboratory that created the mouse-human comparative molecular map, with Drs Neal Copeland and Nancy Jenkins. (biologists.com)
The PhenoLab was designed for mice, the most common laboratory animal. (bmedia.nl)
Mice are currently the laboratory animal species most closely related to humans for which the knockout technique can easily be applied. (wikipedia.org)
A laboratory mouse in which a gene affecting hair growth has been knocked out (left) is shown next to a normal lab mouse. (wikipedia.org)

Embryonic stem cells are isolated from a mouse blastocyst (a very young embryo ) and grown in vitro . (wikipedia.org)
To appreciate the difference, consider that the old way involves introducing DNA into mouse embryonic stem cells, and then selecting for the rare cells that take up and incorporate the DNA in the right way. (emoryhealthsciblog.com)

The Ddo -/- mice have d-aspartate immunoreactive cells in the cerebellum and adrenal glands that are not observed in the wild-type mice. (johnshopkins.edu)
Also, Ddo -/- mice do not differ from wild-type mice in either passive avoidance or spontaneous alternation tasks. (johnshopkins.edu)

2014) Salty taste deficits in CALHM1 knockout mice. (monell.org)
Ddo -/- mice display deficits in sensorimotor gating and motor coordination as well as reduced immobility in the forced swim test. (johnshopkins.edu)

By causing a specific gene to be inactive in the mouse, and observing any differences from normal behaviour or physiology, researchers can infer its probable function. (wikipedia.org)
The first recorded knockout mouse was created by Mario R. Capecchi , Martin Evans , and Oliver Smithies in 1989, for which they were awarded the 2007 Nobel Prize in Physiology or Medicine . (wikipedia.org)

Rodents are averse to bodies of water, and this aversion has been exploited in experiments designed to study stress in mice. (degruyter.com)
The Cell Phenotyping arm of this module is designed to provide technical expertise and assistance with ongoing cell phenotyping experiments. (utsouthwestern.edu)
Millions of knockout mice are used in experiments each year. (wikipedia.org)

Yet, the mouse shares a similar developmental trajectory to the human heart and a comparable four-chamber morphology that greatly enhances its translational value as a model organism, despite the substantial differences in size (Krishnan et al. (springer.com)

METHODS: A global Abcc5-/- mouse was generated by CRISPR/Cas9. (ox.ac.uk)

Broad phenotypic characterization of emerging mouse models will add important value and greatly enhance their utility to the scientific community. (jax.org)

All reagents and mouse lines described in the paper are freely available through Addgene and the MMRRC repository. (molecularneurobiology.org)

Recent studies demonstrate that similar epigenetic clocks for mice (Mus Musculus) can be slowed by gold standard anti-aging interventions such as calorie restriction and growth hormone receptor knock-outs. (sens.org)

Importantly, the alternative splicing of the LMNA gene was highly sensitive to the levels of specific Serine-Arginine rich (SR) proteins (5) , and produced several splicing isoforms that affect adipose tissue metabolism and aging in opposite manners, with Lamin A and Progerin promoting energy expenditure and aging, while Lamin C favors slower metabolism and moderately slows the aging process in mice (4) . (unil.ch)

Mice produced under the Genome Editing Mice for Medicine (GEMM) Programme funded by the Medical Research Council (UK). (infrafrontier.eu)

The advancement of therapeutic strategies for aquaphobia thus depends on the acquisition of experimental evidence identifying the neurochemical and neuroanatomical pathways underlying phobias [ 15 ], and given their aversion to water, mice are a tempting model organism for investigations into aquaphobia. (degruyter.com)
He convinced me that the mouse was the next big model organism and talked me into joining his lab. (biologists.com)

Sanger Mouse Genetics Project Brown SD, Moore MW (May 2012). (wikipedia.org)
These 360 new disease models that we've identified in mice represent the first steps of a hugely important international project. (sciencedaily.com)
Jarrett comes from the Knock Out Mouse Project (KOMP) at Baylor College of Medicine. (molecularneurobiology.org)

For the first time, human disease traits were seen in mouse models for forms of Bernard-Soulier syndrome (a blood clotting disorder), Bardet-Biedl syndrome (causing vision loss, obesity and extra fingers or toes) and Gordon Holmes syndrome (a neurodegenerative disorder with delayed puberty and lack of secondary sex characteristics). (sciencedaily.com)
To develop our research, we use tissue-specific knockout mice, as well as cellular models of adipocytes. (unil.ch)
We use modern molecular biology, cellular biology and RNA sequencing approaches, in addition of metabolically phenotyping our conditional knockout models. (unil.ch)
The internationally recognized program that she leads at Baylor has generated hundreds of new mouse models of human disease, which have enabled discoveries of gene functions in diverse areas, including cancer, reproduction, neurobiology, obesity, and blood, heart and bone development. (biologists.com)
[3] Many mouse models are named after the gene that has been inactivated. (wikipedia.org)
Other mouse models are named according to their physical characteristics or behaviours. (wikipedia.org)
EMMA has manually curated the information of several archived mouse strains reported to constitute specific disease models. (infrafrontier.eu)

Blastocysts containing cells, that are both wildtype and knockout cells, are injected into the uterus of a foster mother. (wikipedia.org)
The chimera mice are crossed with a normal wildtype mouse (grey). (wikipedia.org)

Recently, I have been studying mice fed cafeteria diets to understand the contribution of food choice to the development of human obesity. (monell.org)
Examples of research in which knockout mice have been useful include studying and modeling different kinds of cancer , obesity , heart disease , diabetes , arthritis , substance abuse , anxiety , aging and Parkinson's disease . (wikipedia.org)

The goal of the Cell Culture and Cell Phenotyping Module is to streamline cell culture and phenotyping analyses for vision researchers. (utsouthwestern.edu)
Consequently, observing the characteristics of knockout mice gives researchers information that can be used to better understand how a similar gene may cause or contribute to disease in humans. (wikipedia.org)

This is a trans-NIH initiative to creete quality mouse knockouts. (nih.gov)
The set-up of a Rat Phenotyping Center is another initiative which is enthusiastically discussed among the IOP partners. (bmedia.nl)

By looking at the mouse's resulting characteristics in a variety of standardised tests, the team then see if and how the gene knockout manifests itself as a disease, and link their findings to what is already known about the human version of the disease. (sciencedaily.com)
Human - Mouse: Disease Connection search refinements. (jax.org)
genome sequence, is frequently used to generate knockout or knockin strains, and is a well-established model of influenza disease disease. (bassresearch.com)

Prior to this, gene knockout technology was based on ES cells. (mousephenotype.org)
For this example, we will take stem cells from a white mouse. (wikipedia.org)
Our previous protocol to differentiate human induced pluripotent stem cells (iPSCs) into skin-derived precursor cells (SKPs) as a source of dermal stem cells employs mouse fibroblasts as feeder cells and is therefore unsuitable for clinical use. (bvsalud.org)
Then the ES cells have to be injected into a blastocyst, followed by mouse breeding to "go germline. (emoryhealthsciblog.com)
If you want your favorite gene to flip around and produce a fluorescent protein when you give the mice an antibiotic, but only in some cells - Ingenious can do that. (emoryhealthsciblog.com)

The Asian Mouse Mutagenesis Useful resource Affiliation (AMMRA) is a non-profit group consisting of main useful resource and analysis establishments with rodent experience from inside the Asia Pacific area. (eumorphia.org)

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Chemicals and Drugs1

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Psychiatry and Psychology1

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Information Science1

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IMPC17

Phenotype5

Genes15

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KOMP22

C57BL5

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Conditional knockouts2

Phenotypic data2

Single gene3

Behavioural3

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Assays1

Rats3

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EUCOMM1

Behavioral2

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Laboratory4

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