• YAP siRNA and ranibizumab, a VEGF monoclonal antibody, were injected intravitreally in CNV mice. (molvis.org)
  • The following product was used in this experiment: CD31 (PECAM-1) Monoclonal Antibody (WM-59 (WM59)), Functional Grade, eBioscience™ from Thermo Fisher Scientific, catalog # 16-0319-82, RRID AB_468934. (thermofisher.com)
  • Description: The WM59 monoclonal antibody reacts with human CD31, also known as platelet-endothelial cell adhesion molecule-1 (PECAM-1) and gpIIa. (thermofisher.com)
  • Immunofluorescent double staining was used to detect YAP cellular localization with CD31 (an endothelial cell marker) antibody. (molvis.org)
  • Induced EndoMT cells exhibited up-regulation of mesenchymal markers, including collagen type I and α-smooth muscle actin, and a reduction in endothelial cell and junctional proteins, including von Willebrand factor, CD31, occludin, and vascular endothelial-cadherin. (surrey.ac.uk)
  • CD31 (platelet endothelial cell adhesion molecule-1, PECAM-1) is an inhibitory coreceptor involved in regulation of T cell and B cell signaling by a dual immunoreceptor tyrosine-based inhibitory motif (ITIM) that upon associated kinases-mediated phosphorylation provide docking sites for protein-tyrosine phosphatases. (thermofisher.com)
  • Histologic assessment of patients with systemic sclerosis-associated PAH and the hypoxia/SU5416 mouse model identified the presence von Willebrand factor/α-smooth muscle actin-positive endothelial cells in up to 5% of pulmonary vessels. (surrey.ac.uk)
  • Serum collected from tasquinimod or vehicle control treated MM- bearing mice two weeks after tumor cell inoculation, was subjected to a Mouse Angiogenesis Proteome Profiler Antibody Array (R&D). A significant decrease in serum levels of pro-angiogenic factors including VEGF, FGF2, tissue factor, and endoglin was detected in tasquinimod-treated mice. (aacrjournals.org)
  • Using mouse genetic models, the authors show that Schwann cells regulate vascularization of the sciatic nerve and are required for a decrease in vascular density postnatally. (elifesciences.org)
  • Mating of Tie2-Cre males to females carrying a floxed AR gene produced Vascular Endothelial Androgen Receptor Knockout (VEARKO) mice and littermate controls. (biomedcentral.com)
  • CD31 is expressed ubiquitously within the vascular compartment and is located mainly at junctions between adjacent cells. (thermofisher.com)
  • Using knockin mice expressing functionally stunted β 3 integrin, we show that bone marrow transplantation rescues impaired angiogenesis in these mice by normalizing BMDC recruitment. (rupress.org)
  • Furthermore, M2 polarization and angiogenesis were induced upon the administration of exosomes in mouse Matrigel plug and hindlimb ischemia (HLI) models. (biomedcentral.com)
  • CD31 is a multifunctional molecule with diverse roles in modulation of integrin-mediated cell adhesion, transendothelial migration, angiogenesis, apoptosis, negative regulation of immunoreceptor signaling, autoimmunity, macrophage phagocytosis, IgE-mediated anaphylaxis and thrombosis. (thermofisher.com)
  • LTα1β2 induced expansion of FRCs and CD31+Pdpn+ lymphoid endothelial cells, corresponding to the phenotypic characteristic of stromal cells in NPs with eLTs. (authorea.com)
  • Tumor-bearing mice were randomly assigned to treatment or control groups. (aacrjournals.org)
  • We next utilized S100A9 knockout (KO) mice to address the question of whether the anti-tumor effect of tasquinimod is mediated through inhibition of S100A9. (aacrjournals.org)
  • Intraperitoneal injection of si-PRNP with fucoidan reduced proliferation and tumor volume in Balb/c nude mice. (iiarjournals.org)
  • Thus, in tumor-bearing mice, 60% tumor reduction was observed, while in metastatic mice, the number of nodules decreased by 25% compared to the control group. (researchgate.net)
  • Moreover, I. obliquus extract-treated mice demonstrated the increase in tumor agglomeration and inhibition of vascularization. (researchgate.net)
  • Furthermore, I. obliquus prevented temperature drop in mice after tumor implantation. (researchgate.net)
  • B7H6-particular BiTE therapy improved the success of lymphoma bearing mice and reduced tumor burden SF3a60 of melanoma and ovarian tumor bearing mice. (colinsbraincancer.com)
  • Mouse T cell lymphoma range RMA, melanoma cell range B16F10, and ovarian tumor cell line Identification8 have already been referred to previously (17C19). (colinsbraincancer.com)
  • Mouse T cell lymphoma range RMA/B7H6, melanoma cell range B16F10/B7H6, ovarian tumor cell line Identification8/B7H6 were produced by retrovirus transduction of their parental range RMA, B16F10, or Identification8 cells, respectively, using dualtropic retroviral vectors including the human being gene relating to protocols previously referred to (17). (colinsbraincancer.com)
  • Material and Strategies Mice C57BL/6 mice had been purchased through the National Cancers Institute (Frederick, MD). Mice had been used in test at age 6C12 weeks outdated. (colinsbraincancer.com)
  • For the in vivo studies, Plasmodium berghei ANKA (PbA)-infected CBA and C57BL/6 mice have been used as a CM susceptible model. (biomedcentral.com)
  • Muse cells do not express CD34 (markers for hematopoietic stem cells, adipose stem cells, VSELs) and CD117 (hematopoietic stem cells markers), Snai1 and Slug (skin-derived precursors markers), CD271 and Sox10 (neural crest-derived stem cells markers), NG2 and CD146 (perivascular cells) or CD31 and von Willebrand factor (endothelial progenitor markers). (wikipedia.org)
  • In this model, mouse MM cells, injected intravenously, home to the BM and grow as MM that closely resembles the human disease. (aacrjournals.org)
  • Xenograft models of human MM established in immunodeficient SCID-beige mice were utilized to exclude the involvement of B-, T- and NK cells in the pro-survival effect of tasquinimod. (aacrjournals.org)
  • En face co-immunostaining of the mouse aortic arch revealed a low level of PDCD4 in endothelial cells undergoing pulsatile shear stress. (plos.org)
  • F-G) GLI3 was detected by immunostaining in jejunum (H) but not in the CV taste cells of Skn-1a knockout mice. (health-ground.com)
  • Systemic transplantation of an enriched CXCR2 population of mouse adipose-derived stem cells (mADSC) into DEB-affected mice demonstrated effective recruitment of cells to the blistering skin under the influence of blister-derived ligands and deposition of therapeutic type VII collagen. (biomedcentral.com)
  • PTPH1 dephosphorylates TCRzeta in vitro , inhibiting the downstream inflammatory signaling pathway, however no immunological phenotype has been detected in primary T cells derived from PTPH1-KO mice. (biomedcentral.com)
  • After a constitutive activation of the Akt pathway was initiated, early follicles (50-150 um) were mechanically isolated from 8-day-old mice and co-cultured with these activated ovarian endothelial cells (AOEC) ( n = 32), gel ( n = 24) or within matrigel ( n = 27) in serum free media for 14 days. (biomedcentral.com)
  • A Cre-inducible fluorescent reporter line was used to empirically establish the utility of a mouse line expressing Cre Recombinase driven by the Tie2-Promoter, for targeting VE cells. (biomedcentral.com)
  • We demonstrate the successful generation and empirical validation of a cell-specific knockout of AR from VE cells, and conclude that AR expression in VE cells is not essential for spermatogenesis or male fertility. (biomedcentral.com)
  • Batf knockout inhibited the differentiation of T helper (Th)17 cells and the conversion of CD4 + Foxp3 + cells to CD4 + IL-17 + cells. (biomedcentral.com)
  • CRISPR/Cas9-mediated TPM1 knockout in human induced pluripotent stem cells (iPSCs) enhanced hematopoietic progenitor development, increasing total megakaryocyte and erythroid cell yields. (biomedcentral.com)
  • Stromal cells, with an expansion of CD31-Pdpn+ fibroblastic reticular cell (FRC) type, were the major source of CXCL13 in NPs without eLTs. (authorea.com)
  • Commensurate with the known truth that HuR improved the great quantity of adhesion substances VLA-4 on Th17 cells, knockout of HuR impaired splenic Th17 cell migration towards the central anxious program and abolished the condition. (globaltechbiz.com)
  • In vivo R12-31 agonist activity was demonstrated by its ability to induce the formation of intestinal villous microfold cells in mice. (cnrs.fr)
  • 0.0001) reduced growth of H929 and RPMI-8226 tumors in these mice. (aacrjournals.org)
  • Cytokine release, inflammatory pain and gene expression were investigated in challenged PTPH1-WT and KO mice. (biomedcentral.com)
  • In another study by Brown and Ruoslahti in 2004, phage display strategy was employed in a mouse model of breast cancer metastasis, which revealed that a gene identical to AEG-1 was expressed at high levels in relation to metastasis of breast cancer, and the identified gene was named metadherin after its proposed role in promoting homing of breast cancer cells to the lungs [ 2 ]. (biomedcentral.com)
  • IL-17A gene knockout, and CXCL13 and LTβR blockage diminished nasal eLT formation in the murine model. (authorea.com)
  • METHODS: The suppressive effect of kaempferol administration on skin inflammation was examined using MC903-induced atopic dermatitis-like skin inflammation mouse model. (bvsalud.org)
  • Main methods: Anticancer activity of the I. obliquus extract was examined in mouse models of Lewis lung carcinoma growth and spontaneous metastasis after 3 weeks of continuous extract intake at the dose of 6 mg/kg/day, which corresponded to that ingested daily with Chaga infusion in Japan. (researchgate.net)
  • Mouse ovarian ECs were isolated using magnetic cell sorting or by FACS and cultured in serum free media. (biomedcentral.com)
  • We demonstrate that co-culture with ovarian ECs enables culture of primary and early secondary mouse ovarian follicles. (biomedcentral.com)
  • In murine models of melanoma and lymphoma, tumours were smaller in VAP-1 knock-out mice compared to wild-type littermates [ 9 ] as a consequence of reduced myeloid suppressor cell recruitment. (biomedcentral.com)
  • The present study shows that carrageenan induces a trend of slightly increased spontaneous pain sensitivity in PTPH1-KO mice compared to WT (wild-type) littermates, but no differences in cytokine release, induced pain perception and cellular infiltration have been detected between the two genotypes in this mouse model. (biomedcentral.com)
  • We exposed healthy mouse lung tissue to radiation before the induction of metastasis and observed a strong enhancement of cancer cell growth. (nature.com)
  • In the study, a mouse CNV model was generated by laser photocoagulation. (molvis.org)
  • Nuclear positioning, germinal vesicle breakdown, spindle migration, spindle rotation, chromosome segregation, and polar body extrusion are the most critical cellular processes during oocyte meiosis I and II, and a growing number of studies primarily using the mouse oocyte model revealed that actin filaments were critical for these processes, especially for spindle migration. (bioone.org)
  • In this study, we observed increased 11β-HSD1 expression in osteoclasts within an osteoporotic mice model (ovariectomized mice). (ijbs.com)
  • The mouse model manifests the neurological symptoms within 6-14 days after infection and then dies [ 10 ]. (biomedcentral.com)
  • Comprehensive proteomic screening of chemokines in the blister fluids of DEB-affected mice was conducted to define the inflammatory and immune activities, thus providing potential to examine local biological mechanisms and define the protein signature within lesional skin as a potential marker of disease activity. (biomedcentral.com)
  • Also, we showed that intracutaneous transplantation of genetically normal ASC from bone marrow into DEB-affected mouse skin restores the DEJ via direct donation of the therapeutic type VII collagen [ 8 ]. (biomedcentral.com)
  • Then, 11β-HSD1 global knock-out or knock-in mice were employed to demonstrate its function in manipulating bone metabolism, showing significant bone volume decrease in 11β-HSD1 knock-in mice. (ijbs.com)
  • Meanwhile, we found 11β-HSD1 facilitated mature osteoclasts formation inhibited bone formation coupled H type vessel (CD31 hi Emcn hi ) growth through reduction of PDFG-BB secretion. (ijbs.com)
  • In addition, TMZ effectively inhibited EndMT in ISO-treated rat hearts and TGF-β-treated HUVECs, as manifested by increased CD31 expression, decreased α-SMA expression, and suppressed cell migration. (dovepress.com)
  • Proliferating cell nuclear antigen (PCNA) expression in CNV mice without or with YAP siRNA intravitreal injection and the colocalization of PCNA and CD31 were measured with western blotting and immunofluorescent double staining, respectively. (molvis.org)
  • We found that both antibodies recognized mouse RANK with high affinity, while RANK-02 and R12-31 recognized human RANK with high and lower affinities, respectively. (cnrs.fr)
  • We challenged PTPH1-KO mice with two potent immunomodulatory molecules, carrageenan and LPS, in order to determine PTPH1 possible role in inflammatory response in vivo . (biomedcentral.com)
  • CIA and K/BxN serum transfer were used to generate inflammatory arthritis models in wild-type (WT) and Batf −/− mice. (biomedcentral.com)
  • The anti-mouse Compact disc3 hybridoma 145.2C11, K562 was from American Type Tradition Collection (Manassas, VA). The B3Z T cell hybridoma was from Dr. Nilabh Shastri (College or university of California at Berkley). (colinsbraincancer.com)
  • She serves on NIH study sections and as reviewer for journals in her area of expertise, manages an active laboratory, supervises students in pulmonary biology and epithelial cell research and has received awards recognizing both teaching and original research accomplishments. (rochester.edu)
  • N-terminal Ig-like domain of CD31 is responsible for its homophilic binding, which plays an important role in cell-cell interactions. (thermofisher.com)
  • We found, in mice, that the sciatic nerve invasion by blood vessels begins around embryonic day 16 and continues until birth. (elifesciences.org)
  • Interestingly, I. obliquus intake decreased body weight in middle-aged mice and increased body temperature in response to light-dark switching in mature adult mice. (researchgate.net)
  • Registered users receive a variety of benefits including the ability to customize email alerts, create favorite journals list, and save searches. (bioone.org)
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  • Primordial and primary follicles receive their nutrition and oxygen supply via passive diffusion from stromal blood vessels. (biomedcentral.com)
  • Administration of tasquinimod did not improve survival of MM-bearing S100A9 KO mice. (aacrjournals.org)