Knockout mice obese. Medical search. Frequent questions

reported that both visceral and subcutaneous adipocytes from obese human beings and mice portrayed all MHCII elements necessary for antigen display and increased degrees of Compact disc80 and Compact disc86, and could work as APCs therefore. (exposed-skin-care.net)
Indeed, the principal adipocytes isolated from obese mice could induce antigen-specific Compact disc4+ T cell activation (58). (exposed-skin-care.net)
further defined that mostly huge adipocytes from obese adipose tissues exhibited an increased expression degree of MHCII substances and acted as APCs to activate Compact disc4+ T cells to secrete IFN- (103). (exposed-skin-care.net)
Taken together, one important mechanism for obese adipose CD4+ T cell activation may be mediated through MHCII expressed on ATMs and adipocytes. (exposed-skin-care.net)
In non-obese subjects, adipocytes release higher levels of adiponectin that improves insulin sensitivity and enhances M2 macrophage polarization [ 15 ]. (biomedcentral.com)
Obese adipocytes overexpress MHC class II molecules and costimulators to act as antigen-presenting cells (APCs) and promote the activation of CD4 + T cells. (frontiersin.org)
From these results, two new functions of sulforaphane have been uncovered: (1) ameliorating obesity by browning of adipocytes to augment energy consumption and (2) improving 'obese' gut bacterial flora caused by high-fat diet and metabolic endotoxemia. (medicalxpress.com)
We will create mice that overexpress or lack ChREBP selectively in adipocytes. (grantome.com)
Because reduced ChREBP expression in adipose tissue of obese humans correlates highly with insulin resistance, understanding the mechanisms that regulate ChREBP in adipocytes could lead to novel therapeutic approaches to prevent and treat type 2 diabetes. (grantome.com)
In adipocyte Senp2-de﫿ciency mice, accumulation of the SUMOylated Setdb1 suppressed the expression of Pparg and Cebpa genes as well as lipid metabolism-related target genes, which would decrease the ability of lipid storage in adipocytes. (deepdyve.com)
2003). Selective Cellular uptake of fatty acids and following storage in the form of disruption of Pparγ2 or adipocyte-speci﫿c Pparγ knockout leads TGs in adipocytes are key steps in lipid storage. (deepdyve.com)
Sorl1 gene knockout in mice accelerated the breakdown of triacylglycerides in adipocytes and protected the animals from diet-induced obesity. (medscape.com)

MRAP2/MC4 double knockout transgenic mice were found morbidly obese. (wikipedia.org)
To this end we used a HILPDA tissue-specific knockout mice model in macrophages and hepatocytes generated by using LysM-Cre and Alb-Cre transgenic mice, respectively. (wur.nl)
Here, we explored the role of NPC1L1 in colorectal tumorigenesis in vivo by using transgenic mice. (biomedcentral.com)
Furthermore, we demonstrate that GFAP-MAOB transgenic mice, in which MAOB is over-expressed under the GFAP promoter for astrocyte-specific over-expression, display exacerbated MPTP-induced tonic inhibition and parkinsonian motor symptoms compared to wild-type mice, indicating the importance of astrocytic MAOB for parkinsonian motor symptoms. (en-journal.org)

The rodent single gene obesities represent complex admixtures of the key mechanisms that are involved in the development of an obese phenotype, namely an increase in energy intake, relative decrease in energy expenditure, or preferential partitioning of ingested energy to fat storage. (bmj.com)
The investigation of adipose macrophage phenotype in obese myeloid IGF1R knockout (MIKO) mice revealed a reduction in transcripts associated with M2-like macrophage activation. (umn.edu)
An obese phenotype is also observed in BDNF-conditional knockout mice, where BDNF is deleted after birth and the knockout is restricted to the brain ( 216 ). (hyperthermicwellness.com)
Senp2 knockout mice exhibited a lipodystrophy-like phenotype. (deepdyve.com)

In diet-induced obese mice, HILPDA deficiency in macrophages markedly reduced lipid accumulation in macrophages yet it did not alter any measured inflammatory or metabolic parameters. (wur.nl)
Another statement showed the preadipocyte- and endothelial cell-derived stromal-derived element-1 (CXCL12), mediated early infiltration of CD4+ T lymphocytes in obesity, which preceded the increase of macrophages in adipose cells of mice on HFD (101). (exposed-skin-care.net)
The density of M2 macrophages is lower compared to M1 macrophages in obese patients [ 16 ] as a result of imbalance in CCR2/CCL2 and CCR5 axis. (biomedcentral.com)
Ablation of IGF1 receptors from myeloid cells reduced phagocytosis, increased macrophages in adipose tissue, elevated adiposity, lowered energy expenditure, and led to insulin resistance in mice fed a high-fat diet. (umn.edu)
Surprisingly, cold challenge did not trigger an overt M2-like state and failed to induce tyrosine hydroxylase expression in adipose tissue macrophages of control or MIKO mice. (umn.edu)
The WAT of P311 knockout (KO) mice has fewer resident macrophages and decreased cellular dynamics, including decreased autophagy and apoptosis. (hhs.gov)

Adiponectin plasma levels and adipose-tissue gene expression were significantly lower in obese subjects and inversely correlated with obesity-associated variables, including high-sensitive C-reactive protein (hs-CRP) and interleukin-6 (IL-6). (diabetesjournals.org)
However, when the knockout mice become obese with age, they develop fasting hyperglycemia and glucose intolerance, while neither fasting hyperglycemia nor glucose intolerance is evident in the aged knockout mice without obesity, suggesting that both the genetic defect in glucose-induced insulin secretion and the acquired insulin resistance due to environmental factors are necessary to develop diabetes in Kir6.2 knockout mice. (diabetesjournals.org)
On the other hand, since Nrf2-knockout mice fed with high-fat food supplemented with sulforaphane did not show reduced body weight increase or fat burning through adipose tissue browning, the importance of Nrf2 is now clearly shown as the target molecule of sulforaphane for ameliorating obesity. (medicalxpress.com)
Naoto Nagata et al, Glucoraphanin Ameliorates Obesity and Insulin Resistance Through Adipose Tissue Browning and Reduction of Metabolic Endotoxemia in Mice, Diabetes (2017). (medicalxpress.com)
In various mouse and rat models of obesity and type 1 and 2 diabetes mellitus, eCBs generated in various renal cells activate CB 1 receptors and contribute to the development of oxidative stress, inflammation, and renal fibrosis. (degruyter.com)
By using knockout mice specifically in brown adipose tissue, this project aims at dissecting the role of Suv420h proteins in the etiology of obesity. (europa.eu)
40% fat) for 5 weeks, b1 KO mice were more susceptible to obesity than WT controls and failed to develop diet-induced thermogenesis as assessed by BAT Ucp1 mRNA levels and oxygen consumption. (deiodinase.org)

Obese, hypertensive, type 2 diabetic rats SHR/NDmcr-cp were given, for 12 weeks, either a normal, middle-carbohydrate/middle-fat diet (MC/MF group) or a high-carbohydrate/low-fat diet (HC/LF group). (hindawi.com)
TREM-1 expression is significantly increased along with the M1 markers in liver biopsy of obese diabetic (17/17) and obese non-diabetic patients (9/16). (biomedcentral.com)
The possibility that leptin has a regulatory effect on Foxm1 expression is usually further supported by non diabetic C57BL/6 leptin (ob/ob) mouse model since these animals developed up regulation of islets Foxm1 (17). (irjs.info)
Recently, there is a report that NNMT expression is elevated in WAT and liver of obese and diabetic mice. (checkpointsignaling.com)

Similarly, in diet-induced NASH mice, HILPDA hepatocyte deficiency modestly yet significantly reduced liver triglyceride accumulation and plasma ALT levels. (wur.nl)
A metabolomic analysis of omega-3 fatty acid-mediated attenuation of western diet-induced nonalcoholic steatohepatitis in LDLR-/- mice. (colorado.edu)
Docosahexaenoic acid attenuates Western diet-induced hepatic fibrosis in Ldlr-/- mice by targeting the TGFβ-Smad3 pathway. (colorado.edu)
Docosahexaenoic acid attenuates hepatic inflammation, oxidative stress, and fibrosis without decreasing hepatosteatosis in a Ldlr(-/-) mouse model of western diet-induced nonalcoholic steatohepatitis. (colorado.edu)
Down-regulation of p63 attenuates liver steatosis in p53 knockout mice and in diet-induced obese mice, whereas the activation of p63 induces lipid accumulation. (sabiolab.com)
β(1) Adrenergic receptor is key to cold-and diet-induced thermogenesis in mice. (deiodinase.org)

In obese humans, adipocyte-secreted CCL20 may contribute to the deposition of Compact disc4+ helper and Compact disc8+ cytotoxic T lymphocytes within adipose tissues, possibly via connections with CCR6 which was upregulated on T cells in obese adipose tissues (100). (exposed-skin-care.net)
Although less fat stor- adqcKO pose lipid storage in adipocyte-speci﫿c Senp2 knockout mice fed age was shown in Senp2 adipose tissues, the increased with high-fat diets (HFD). (deepdyve.com)

Gene expression studies performed on PPARα null mice have shed light into a variety of genes regulated by PPARα. (wur.nl)
The research contained in this thesis first sought to improve our current knowledge on the transcriptional regulation by peroxisome proliferator-activated receptors (PPAR)-α activation on human liver in vivo using a novel humanized-liver mouse model. (wur.nl)
The role of PPARα in gene regulation in mouse liver is well characterized. (wur.nl)
Model systems to study PPARα in human liver vary from hepatoma cell lines, human primary hepatocytes, human precision cut liver slices, and mice expressing human PPARα. (wur.nl)
A novel model to study in vivo PPARα activation in human liver is a chimeric mouse carrying human liver cells. (wur.nl)
Quantitative PCR indicated that induction of PPARα targets by fenofibrate was less pronounced in the human hepatocytes than in the residual mouse hepatocytes. (wur.nl)
Our results support the major role of PPARα in regulating hepatic lipid metabolism, highlight the more modest effect of PPARα activation on gene regulation in human liver compared to mouse liver, and indicate that PPARα may have a suppressive effect on DNA synthesis in human liver. (wur.nl)
Recently, impaired insulin secretion has been reported in heterozygous PPAR-deficient mice, which is related to increased islet triacylglycerol content (16). (irjs.info)

In this research, we performed transcriptomics analysis on the effect of fenofibrate in mice with hepatocyte-humanized livers and compared the results with other relevant transcriptomics datasets. (wur.nl)
In contrast, pathways connected to DNA synthesis were downregulated by fenofibrate in chimeric mice with hepatocyte humanized livers yet upregulated by fenofibrate in normal mouse livers. (wur.nl)
Expression of TAp63, IKKβ and XBP1s is also increased in livers of obese patients with NAFLD. (sabiolab.com)
Lately, FBXO2 was reported to become up-regulated in the livers of obese mice, as well as the insulin receptor was defined as a substrate of FBXO2 [37]. (researchassistantresume.com)

In contrast, certain immunodeficient mouse models infected with mouse-adapted DENV strains show signs of severe disease similar to the 'vascular-leak' syndrome seen in severe dengue in humans. (mdpi.com)

In conclusion, our data suggest a transcriptional mechanism leading to decreased adiponectin plasma levels in obese women and demonstrate that low levels of adiponectin are associated with higher levels of hs-CRP and IL-6, two inflammatory mediators and markers of increased cardiovascular risk. (diabetesjournals.org)

NPC1L1 −/− mice had significant lower intestinal inflammation scores and expressed inflammatory markers p-c-Jun, p-ERK and Caspase-1 p20 lower than wild-type. (biomedcentral.com)
NPC1L1 knockout also reduced lymphadenectasis what may be caused by inflammation. (biomedcentral.com)
NPC1L1 knockout decreasing plasma lipid, especially cholesterol, to reduce inflammation and decreasing β-catenin, p-c-Jun and p-ERK may be involved in the mechanism. (biomedcentral.com)

However, knocking down the elevated ChREBP expression in liver of obese mice improves insulin sensitivity and metabolic syndrome. (grantome.com)

The results of our study showed over-expression of TREM-1, M1 markers and down-regulation of TREM-2 and M2 markers in the omentum, subcutaneous and liver biopsies of obese patients (diabetics and non-diabetics) compared to non-obese patients. (biomedcentral.com)
Mice with liver-specific p53 deletion develop steatosis and show increased levels of p63. (sabiolab.com)

One article published in Nature in 2006, described a microbiome sequence comparison of obese vs. lean mice , finding the ratios of different phyla between the two mice strains. (phagehunter.org)

adqcKO Mechanistically, adipocyte Senp2 de﫿ciency caused the downregula- Senp2 mice exhibit an ectopic lipid accumulation and tion of Pparg and Cebpa as well as their downstream target genes insulin resistance related to lipid storage. (deepdyve.com)

Fenofibrate increased the size of the mouse but not human hepatocytes and tended to reduce steatosis in the human hepatocytes. (wur.nl)
The researchers found that the mice fed with sulforaphane exhibited weight gain rate 15 percent lower than the mice fed without sulforaphane, 20 percent visceral fat reduction, and reduction of augmentation of their hepatic steatosis and blood glucose level . (medicalxpress.com)

Wild-type mice and NPC1L1 −/− (NPC1L1 knockout) mice were treated with azoxymethane (AOM)-dextran sodium sulfate (DSS) to induce colitis-associated colorectal tumorigenesis. (biomedcentral.com)
NPC1L1 was highly expressed in the small intestine of wild-type mice but its expression was undetectable in colorectal mucous membranes or tumors in either group. (biomedcentral.com)
The introduced gut flora induced metabolic dysfunction in these wild-type mice identical to that of the TLR5 knockouts. (phagehunter.org)

Notably, researchers speculated that neither lean mice nor genetically engineered mice (mice known as "beta(3)-AR knock-out mice") appeared to react to the lipolytic effects of AOD 9604 [vi]. (asianage.com)
Diverse roles of K(ATP) channels learned from Kir6.2 genetically engineered mice. (diabetesjournals.org)
To determine the physiological roles of K(ATP) channels directly, we have generated two kinds of genetically engineered mice: mice expressing a dominant-negative form of Kir6.2 specifically in the pancreatic beta-cells (Kir6.2G132S Tg mice) and mice lacking Kir6.2 (Kir6.2 knockout mice). (diabetesjournals.org)

Thus, Kir6.2G132S Tg mice and Kir6.2 knockout mice provide a model of type 2 diabetes and clarify the various roles of K(ATP) channels in endocrine pancreatic function. (diabetesjournals.org)
When the IRS genes are deliberately taken out of action in so-called "knockout" mice, type 2 diabetes results. (biologywriter.com)
This would explain why obese people so frequently develop type 2 diabetes. (biologywriter.com)
1 As obese children are more likely to become obese adults, 2 we may expect to see profound public health consequences as a result of the emergence in later life of associated co-morbidities such as non-insulin dependent diabetes mellitus, ischaemic heart disease, and stroke. (bmj.com)
Summary One third of the US population is clinically obese, a condition which increases the risk for chronic diseases like type 2 diabetes mellitus (DM), heart disease and cancers. (hhs.gov)
mice and leptin receptor insufficiency in mice resulted in hyperinsulinemia even prior to the development of weight problems and diabetes which hyperinsulinemia was ameliorated by administration of recombinant leptin towards the mice. (irjs.info)

In mice, overexpression of human SORLA in murine adipose tissue blocked hydrolysis of triacylglycerides and caused excessive adiposity. (medscape.com)

Interestingly, despite the severe defect in glucose-induced insulin secretion, Kir6.2 knockout mice show only a very mild impairment in glucose tolerance. (diabetesjournals.org)

Unlike previous studies which saw different ratios of bacterial populations between obese and lean mice (ie. (phagehunter.org)

Our results provide the first evidence that NPC1L1 knockout protects against colitis-associated tumorigenesis. (biomedcentral.com)

These processes to severe lipodystrophy and induces insulin resistance in mice (He et al. (deepdyve.com)

indicated that ATMs colocalized with T cells in lymphoid clusters within adipose tissue and may act as APCs, which express high levels of MHCII and also costimulatory molecules and process and present antigens to induce CD4+ T-cell proliferation and activation in adipose tissue of obese mice (29, 68, 105). (exposed-skin-care.net)
showed that adipose tissue from obese mice induced proliferation of splenic CD8+ T cells, indicating a CD8+ T cell-activating environment in obese adipose tissue (31). (exposed-skin-care.net)

A conditional Smg6 mutant mouse model reveals circadian clock regulation through the nonsense-mediated mRNA decay pathway. (achim-kramer-lab.de)

These mice are generated by transplanting human hepatocytes into albumin enhancer-driven urokinase-type plasminogen activator transgenic/severe combined immunodeficiency (uPA/SCID) mice, leading to replacement of the host hepatocytes. (wur.nl)

appropriately, FBXO2-knockout mice develop age-related hearing reduction [36]. (researchassistantresume.com)

Right: CT images revealed that HF+SFN mice had less visceral fat than HF mice upon examination of visceral fat and subcutaneous fat. (medicalxpress.com)

Primary brain tumor initiating cell lines from IDH-wild type GBM stably expressing H2B-Dendra2 were implanted orthotopically in the brains of SCID mice. (nature.com)

The ratio of malignant/tumor in NPC1L1 −/− mice was significantly lower than in wild-type 20 weeks after AOM-DSS treatment. (biomedcentral.com)

Knowing that a mouse with triple b-receptor knockout (KO) is cold intolerant and obese, we evaluated the independent role played by the b1 isoform in energy homeostasis. (deiodinase.org)
Secondly, mice with targeted disruption of the b1 gene (KO of b1 adrenergic receptor (b1 KO)) developed hypothermia during cold exposure and exhibited decreased iBAT thermal response to NE or DB infusion. (deiodinase.org)
The authors describe the metabolic dysfunction of Toll-like Receptor 5 knockout mice. (phagehunter.org)

Similar to CD4+ T cells, CD8+ T cells exhibit effector memory or effector phenotypes expressing elevated levels of IFN- in obese adipose tissue (31, 44). (exposed-skin-care.net)

Induction of Dio2 After Status Epilepticus Modifies Hippocampal Gene Expression in Mice. (deiodinase.org)
NNMT is the most strongly reciprocally regulated gene when evaluating gene expression in white adipose tissue (WAT) from adipose specificLenalidomide Glut4-knockout or adipose-distinct Glut4-over expressing mice with their respective controls. (checkpointsignaling.com)

ChREBP knockout mice have mild diet-related insulin resistance. (grantome.com)

However, expression of macrophage/inflammatory markers and fibrosis were not different between HILPDA knockout and floxed mice. (wur.nl)
The objective of this study was to evaluate the relationship between TREM-1 and macrophage polarization, and association of TREM-1 and M1 macrophage polarization with insulin resistance (IR) in obese population compared to non-obese population. (biomedcentral.com)

In the current study, the researchers of Kanazawa University in collaboration with the researchers of Kagome Co., Ltd. compared the body weight of mice fed with high-fat food supplemented with sulforaphane and others with high-fat food but without sulforaphane. (medicalxpress.com)

Recent studies have revealed numerous potential regulators of adult islet function, many of which are not expressed in mouse or immature human β-like cells. (einsteinmed.edu)
Importantly, these are unique to human beta cell/islet maturation: they are not expressed in adult mouse islet cells or β-like cells from renewable sources. (einsteinmed.edu)

It was determined that the alleged capacity of AOD 9604 to raise the synthesis of beta-3 adrenergic receptors (also known as ADRB3 in obese mice) may have been responsible for this finding. (asianage.com)

Indeed, CD8+ T cells from mouse adipose tissue respond to cytokines and become activated and proliferate under stimulation of IL-12 and IL-18, which are mainly produced by APCs and are elevated in obese adipose tissue (44). (exposed-skin-care.net)
AFP-L3 accumulated in bile duct-like structures in HepG2 cells, and this phenomenon was diminished by FUT8 knockout, suggesting that HepG2 cells have cargo proteins for AFP-L3. (bvsalud.org)
Expectedly, the knockout of the VIP36 gene in HepG2 cells suppressed the secretion of AFP-L3 and other fucosylated proteins, such as fucosylated alpha-1 antitrypsin, into bile duct-like structures. (bvsalud.org)

The imbalance in obese patients due to pro-inflammatory cytokines influences the M1/M2 polarization. (biomedcentral.com)

NPC1L1 −/− mice significantly had fewer tumors than wild-type. (biomedcentral.com)

Characterization and reversal of synaptic defects in the amygdala in a mouse model of fragile X syndrome. (colorado.edu)

Their experiments in lab mice and in human blood and tissue samples further showed that this gene not only is associated with the disease, as predicted computationally, but is also likely to play a major causal role. (stanford.edu)
HNMT is expressed in the central nervous system, where it may play a critical regulatory role because its deficiency is related to aggressive behaviour and abnormal sleep-wake cycles in mice [ 4 ]. (hindawi.com)
Firmicutes vs % Bacteroidetes ), this study found that instead specific species were present or absent in the TLR5 knockouts, indicating a role for TLR5 in maintaining specific characteristics in population of gut microbes. (phagehunter.org)

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