Knockout mice mice cell cycle. Medical search. Frequent questions

Type 2 diabetes mellitus was established in Rnd3 fibroblast-specific knockout and transgenic mice. (thno.org)
K14-EGFP-transgenic mice also exhibit augmented potential for OSCC induction. (biomasswars.com)
Methods Four murine OSCC cell lines, designated MOC-L1 to MOC-L4, are established from tongue tumors induced by 4-nitroquinoline 1-oxide using the K14-EGFP-transgenic mouse model. (biomasswars.com)
To reveal the role of RAGE signaling in AGE-dependent disorders, RAGE knockout cell lines and transgenic animals have been intensively studied [7-10]. (heraldopenaccess.us)

JDP2 plays a role in cell differentiation in several systems. (wikipedia.org)
Ectopic expression of JDP2 inhibits the retinoic acid-induced differentiation of F9 cells and adipocyte differentiation. (wikipedia.org)
By contrast, JDP2 induces terminal muscle cell differentiation in C2 myoblasts and reduces the tumorigenicity of rhabdomyosarcoma cells and restored their ability to differentiate into myotubes. (wikipedia.org)
Methylome mapping suggests that JDP2 plays a role in cell progenitor differentiation of megakaryocytes. (wikipedia.org)
For example, the epithelial−mesenchymal transition phenotype presents more cells with a lower degree of differentiation and greater cell dissociation in the ITF than in other tumor areas 14 , 15 . (nature.com)
The purpose of this study is to identify cell cycle regulators involved in the differentiation of osteoblasts into osteocytes and determine their physiological significance. (lu.se)
The study uses IDG-SW3 cells as a model for the differentiation from osteoblasts to osteocytes. (lu.se)
Among the major cyclin-dependent kinases (Cdks), Cdk1 is most abundantly expressed in IDG-SW3 cells, and its expression is down-regulated during differentiation into osteocytes. (lu.se)
Inhibition of CDK1 activity reduces IDG-SW3 cell proliferation and differentiation into osteocytes. (lu.se)
and cell differentiation ( SOX2 and TGFB3 ) as well as immunohistochemical assay for VEGFA, TP53, Bcl2, TGFB1, and Ki67 protein expression have been performed in 85 FFPE RCC tumor specimens. (hindawi.com)
An aberrant miRNA expression could contribute to cancer development and progression [ 6 , 7 ] and could affect their target genes that are involved in many biological processes, such as cell differentiation, proliferation, apoptosis, metabolism, and development [ 8 ]. (hindawi.com)
It is involved in p53 pathways and is implicated in cell death/survival signaling, the cell cycle, and differentiation, thereby playing a regulatory role in carcinogenesis [ 12 ]. (hindawi.com)
Knockout of Timp1 in mice caused a significant reduction in fibrotic focus formation, collagen fiber deposition, recruitment of fibroblasts, and differentiation of fibroblasts into myofibroblasts in the lungs, indicating that TIMP1 plays a critical role in the pulmonary fibrotic response to MWCNTs. (cdc.gov)
In our previous study, we show that the expression of Znf179 is upregulated during P19 cell neuronal differentiation. (tmu.edu.tw)
Inhibition of Znf179 expression by RNA interference significantly attenuated neuronal differentiation of P19 cells and a primary culture of cerebellar granule cells. (tmu.edu.tw)
Collectively, our results suggest that induction of the Znf179 gene may be associated with p35 expression and p27 protein accumulation, which leads to cell cycle arrest in the G0/G1 phase, and is critical for neuronal differentiation of P19 cells. (tmu.edu.tw)
This includes the analysis of genes in germline stem cell proliferation and differentiation. (ncl.ac.uk)

The CDKN2A/B locus contains genes encoding cell cycle inhibitors, including p16 Ink4a , which have not yet been implicated in the control of hepatic glucose homeostasis. (diabetesjournals.org)
1983 Subsequently cyclin A genes have been found in all multicellular organisms including humans (Pines and Hunter 1990 While only a single cyclin A gene is present in the genomes of and cultured fibroblasts XI-006 or other cell types blocked DNA synthesis consistent with the essential function for cyclin A in DNA replication (Girard et al. (immune-source.com)
At the molecular level, MWCNT exposure significantly increased the expression of the cell proliferation markers Ki-67 and PCNA and a panel of cell cycle-controlling genes in the lungs in a TIMP1- dependent manner. (cdc.gov)
Beyond that, we are linking metabolism to the heart and describing phenotypes that emerge in a set of known genes, when knocked out in mice, such as the leptin receptor ( Lepr ), leptin ( Lep ), and Bardet-Biedl syndrome 5 ( Bbs5 ). (springer.com)
The International Mouse Phenotyping Consortium, IMPC, has set the goal of systematically "knocking out" each protein-coding genes in the mouse and phenotyping these so-called knockout (KO) mice using highly standardized high-throughput tests (Brown and Moore 2012 ). (springer.com)
Using a microarray approach and subsequent functional annotation analysis, we identified the differentially expressed genes in Znf179 knockdown cells and found that several genes are involved in development, cellular growth, and cell cycle control. (tmu.edu.tw)
Ample evidence shows that pro-atherosclerotic disturbed flow induces sustained activation of atherogenic genes in endothelial cells to promote their oxidation, inflammation, cell cycle progression and proliferation, whereas pulsatile shear stress tends to maintain endothelial cells in a quiescent and less proliferative state with a low level of oxidation and inflammation [16] , [17] . (plos.org)
Using the Myc-switchable pIns-MycER TAM ;RIP7-Bcl-x L β-cell tumor model in an unbiased reversible kinetic expression analysis, we previously identified genes required for maintenance of Myc-driven β-cell tumors. (life-science-alliance.org)
These repopulating cells are enriched in disease-associated microglia genes and exhibit similar phenotypic and transcriptional profiles to white-matter-associated microglia. (stanford.edu)
Inactivation of tumor suppressor genes, whose products normally provide negative control of cell proliferation, contributes to malignant transformation in various cell types. (medscape.com)
Like Rb protein, many of the proteins encoded by tumor suppressor genes act at specific points in the cell cycle. (medscape.com)
TP53 activates the expression of genes involved in apoptosis, cell cycle regulation (p21), and MDM2. (medscape.com)
Another important class of tumor suppressor genes involved in cell cycle control and in the generation of human cancers is the cyclin-dependent kinase (CDK) inhibitors. (medscape.com)
The insulin-producing cells that we can make from these patients can then be used to understand the mechanisms by which these risk genes cause dysfunction of beta-cells. (lu.se)

Depletion of JDP2 induces cell death similar to apoptosis. (wikipedia.org)
RNAi-mediated attenuation of Tnfrsf1b decreased TNF-α-induced netrin-1 production and augmented epithelial cell apoptosis in culture. (aacrjournals.org)
Extending these observations, colon biopsies from E. faecalis -colonized IL-10 −/− mice exhibited crypt hyperplasia and increased staining for macrophages, TNF-α, netrin-1, NF-κB, Tnfrsf1b, and the proliferation marker proliferating cell nuclear antigen while also displaying a reduction in epithelial cell apoptosis. (aacrjournals.org)
mice with a mPER2 knockout show a significant increase in tumor development and a significant decrease in apoptosis . (jackkruse.com)
Low PDCD4 level was associated with reduced proliferation but not apoptosis or phosphorylation of endothelial nitric oxide synthase caused by pulsatile shear stress to help maintain the homeostasis of endothelial cells. (plos.org)
Usage of the murine IL-3 dependent Baf3 Pro-B cell line exhibited that overexpression of Snai2 was sufficient to confer resistance to apoptosis induced by growth factor withdrawal which was accompanied by exit from the cell cycle [21]. (bioinf.org)
At the same time, sRAGE is known to compete for RAGE, the main receptor for AGEs, and it has been shown that their interaction contributes to cell apoptosis, as discussed in this review. (heraldopenaccess.us)
The TP53 gene is also capable of stimulating apoptosis of cells containing damaged DNA. (medscape.com)

By contrast, JDP2-knockout mice exhibit in downregulation of p53 and p21 proteins. (wikipedia.org)
Accordingly, cell cycle related proteins, including cyclin D1, PCNA, and CDK4 (cyclin dependent kinase 4) were increased in the liver of ahnak knock-out mice compared wild type mice at 24, 48 and 72hrs after hepatectomy. (snu.ac.kr)
Cardiac fibroblasts (CFs) are the primary source of ECM proteins and the ultimate effector cells in ECM remodeling. (thno.org)

mice represent a useful model for exploring the activation of adult hepatic progenitor cells, since PR-SET7 deficiency leads to cell cycle arrest (Beck knockout mice and investigated the effect of PR-SET7 deficiency in liver organogenesis, hepatocyte proliferation and liver regeneration. (antiviralbiologic.com)
The p19ARF protein, which is encoded by the same locus as p16, also leads to cell cycle arrest by inhibiting the ability of MDM2 to inactivate TP53. (medscape.com)

During cell growth and proliferation, ubiquitin plays an outsized role in promoting progression through the cell cycle. (intechopen.com)
Progression through the cell cycle is driven by the oscillating activity of Cyclin Dependent Kinases (CDKs). (intechopen.com)
Mice lacking the tumor suppressors p16(Ink4a) (Cdkn2a, cyclin-dependent kinase inhibitor 2a), p19(Arf) (an alternative reading frame product of Cdkn2a,), and p27(Kip1) (Cdkn1b, cyclin-dependent kinase inhibitor 1b) result in malignant progression of epithelial cancers, sarcomas, and melanomas, respectively. (koreamed.org)
Some studies showed that blocking cell cycle progression ameliorated fibrosis. (nature.com)
However analyses of cell cycle progression using propidium iodide and anti-BrdU staining revealed that ablation of cyclin A2 increased the portion of cells in S and G2/M phases with concomitant decrease in the G1 populace (Figures 2C and 2D). (immune-source.com)
Although PDCD4 in general suppresses the development and progression of tumors, its specific biological functions differ by cell type [8] . (plos.org)
The CDK4-cyclinD complex normally phosphorylates the retinoblastoma protein (Rb protein), leading to release of the E2F transcription factor and cell cycle progression. (medscape.com)

To investigate the mechanisms of retinal iron regulation, the lab uses conditional knockout mouse models, human retinal tissue, and retinal cell tissue culture. (upenn.edu)
1)Study mechanisms of retinal iron regulation by performing histology and immunofluorescence on retinal sections from conditional knockout mice. (upenn.edu)
Conditional knockout of Gli3 causes a significant larger brain size, which can be recovered by knockdown of miR-7. (frontiersin.org)
1997 In the work explained below we decided to revisit the requirement for cyclin A function in cell proliferation using conditional cyclin A knockout mice. (immune-source.com)
RESULTS Generation and Characterization of Conditional Cyclin A2 Knockout Mice To obtain a conditional cyclin A2 allele we inserted sites into the first and seventh intron of the murine cyclin A2 gene (Physique 1A). (immune-source.com)
Analyses of Cyclin A-null Fibroblasts We next derived fibroblasts from conditional cyclin A2 knockout embryos and cultured them culture (Physique 2A) and normally re-entered the cell cycle from quiescence (Physique 2B). (immune-source.com)

Knockdown of microRNA (miRNA) miR-7 can rescue abnormal brain morphology in Gli3 knockout mice by recovering progenitor specification, neuronal production and migration through a counter-balance of the Gli3 activity. (frontiersin.org)

Mice homozygous for a knock-out allele exhibit embryonic lethality during organogenesis and subtle defects in cell cycle-dependent nuclear movement in neural progenitors. (jax.org)
Our results establish a crucial role for E4F during early embryonic cell cycles and reveal an unexpected function for E4F in mitosis. (cnrs.fr)
Unlike preexisting embryonic stem cell-based knockout mice, our mouse models are free from selectable markers or other external gene insertions, permitting more precise study of cell cycle-related diseases without confounding influences of foreign DNA. (koreamed.org)
E and F) Western blot analyses for p16 in FVB (E) and B6 (F) mouse embryonic fibroblasts. (koreamed.org)
With the continuous development of genetic lineage tracking technology, it has been revealed that cell cycle activity produces polyploid cardiomyocytes during the embryonic, juvenile, and adult stages of cardiogenesis, but newly formed mononucleated diploid cardiomyocytes also elevated sporadically during myocardial infarction. (bvsalud.org)
We research human embryonic stem (ES) cells, germline stem cells and somatic stem cells. (ncl.ac.uk)
Results PR-SET7 deficiency in embryonic hepatocytes impairs liver?organogenesis Mice carrying hepatocyte-specific deletion of in embryonic liver were generated by crossing mice (Oda mice. (antiviralbiologic.com)
Open in a separate window Figure 1 PR-SET7 is required for proper liver organogenesis during embryonic development A Representative pictures of embryos at 18.5?days postcoitum (E18.5) and hematoxylin and eosin staining of whole-mount embryo sections from mice and control littermates (and mRNA levels. (antiviralbiologic.com)

The present study showed that Polo-like kinase 1 (Plk1) was upregulated in the kidneys of CKD patients and mice subjected to unilateral ureteral obstruction (UUO) with location in proximal tubules and tubulointerstitial fibroblasts. (nature.com)
Fibroblasts To rule out the possible contribution from cyclin A1 we crossed cyclin A2f/f mice with cyclin A1-null animals (Liu et al. (immune-source.com)
MWCNT-stimulated cell proliferation was most prominent in fibroblasts but not myofibroblasts. (cdc.gov)
Along these lines, it has been shown that overexpression of mig-6 in Rat2 fibroblasts leads to activation of retinoblastoma resulting in a cell cycle arrest.24 Notably, we found elevated levels of the activator protein-1 transcription factor c-Jun in mig-6 knockout livers after PH. (jnksignaling.com)
We procure skin biopsies from patients, reprogram fibroblasts from these into stem cells, i.e., iPSC, which are then differentiated into insulin-producing beta-like cells. (lu.se)

We tested the role of whole-body molecular imaging in patients with advanced melanoma receiving the CTLA4-blocking antibody tremelimumab, allowing the analysis of changes in glucose metabolism using the PET probe 18 F-FDG and cell replication with the PET probe 3′-deoxy-3′- 18 F-fluorothymidine ( 18 F-FLT). (snmjournals.org)
Scientists working in basic, translational, and clinical cancer metabolism research are invited to join the Academy in New York on April 17th to discuss the intersection between cell signaling and metabolism. (nyas.org)
This enables Leptin to then yoke metabolism and sleep directly to the light and dark cycles of our day. (jackkruse.com)
Myc is a highly pleiotropic transcription factor that governs cell expansion by coordinating diverse cellular processes, including proliferation, dedifferentiation, biosynthetic metabolism, cell growth, and angiogenesis ( Dang, 2012 ). (life-science-alliance.org)
Insulin gene superfamily hormones regulate cell growth, metabolism, and tissue-specific functions. (phoenixbiotech.net)
During my initial postdoctoral years, I became interested in lipid metabolism in beta-cells. (lu.se)
The approach has allowed us to further map and understand metabolism in islets and beta-cells. (lu.se)
We have taken a major interest in how mitochondria are genetically regulated, given their pivotal role in cellular energy metabolism and control of insulin secretion from beta-cells. (lu.se)

A clear example of the critical role of CTLA4 on tolerance is the striking phenotype of CTLA4 knock-out mice, which develop rapid T-cell proliferation and autoimmune infiltration of multiple organs shortly after birth ( 6 , 7 ). (snmjournals.org)
Using mice, the International Mouse Phenotyping Consortium (IMPC) aims to target each protein-coding gene and phenotype multiple organ systems in single-gene knockout models by a global network of mouse clinics. (springer.com)
PHENOTYPE: Mice homozygous for a knock-out allele show increased body weight. (utsouthwestern.edu)

TNF-α-mediated bystander assays used a murine coculture system of primary colonic epithelial cells and E. faecalis -infected macrophages ( in vitro ), with an interleukin 10 (IL-10)-deficient mouse model of colon cancer that involves long-term colonization with E. faecalis ( in vivo ). (aacrjournals.org)
In vitro, the expression of Hes1 was higher in confluent cells maintained under high calcium conditions. (molvis.org)
Because dengue and Zika viruses co-circulate in the urban Aedes aegypti mosquito-human cycle, a logical question, as suggested by in vitro data, is whether dengue virus infections result in antibody-dependent enhancement of Zika virus infections. (cdc.gov)

In addition, Plk1 also prevented partial epithelial-mesenchymal transition (pEMT) of tubular epithelial cells via autophagy pathway. (nature.com)
In this study, we explored the role of Plk1 in the classical kidney fibrosis model, UUO, and cultured fibroblast and renal tubular epithelial cells by using specific Plk1 inhibitors, genetic silencing, and heterozygous global knockout mice. (nature.com)
Brumell JH, Tang P, Zaharik ML, Finlay BB: Disruption of the Salmonella -containing vacuole leads to increased replication of Salmonella enterica serovar typhimurium in the cytosol of epithelial cells. (pcna-signal.com)

cyclin A function was essential for proliferation of hematopoietic and embryonal stem cells. (immune-source.com)
In these compartments cyclin A-Cdk complexes are expressed at particularly high levels which may render stem cells dependent on cyclin A. INTRODUCTION Replication of genetic material during cell division in Metazoan organisms is thought to be driven by cyclin A. Cyclin A was the first cyclin cloned in any organism (Swenson et al. (immune-source.com)
Such continuously renewing epithelia are by definition governed by stem cells. (molvis.org)
Upon division, the slow cycling limbal epithelial stem cells give rise to daughter cells known as transient amplifying (TA) cells. (molvis.org)
Recent progress in this field includes the generation of sRAGE secreting stem cells, showing promising efficacy in the treatment of various diseases, such as cardiovascular, metabolic, and neurodegenerative disorders. (heraldopenaccess.us)
The translation of stem cells is showing their therapeutic strength for a wide range of intractable and chronic diseases in both clinical and academic studies [1]. (heraldopenaccess.us)
One example of a gene-edited cell line isthe soluble receptor for advanced glycation end product (sRAGE) secreting stem cells, and much attention is paid to the remedial role of sRAGE molecules in advanced glycation end product (AGE)-associated disorders [3-6]. (heraldopenaccess.us)
their combination with stem cells is among the best alternatives for their continuous generation in vivo . (heraldopenaccess.us)
Our most recent initiative is having established a platform for induced pluripotent stem cells (iPSC). (lu.se)

Monoclonal antibodies blocking CTLA4 induce regression of immunogenic tumors in mice ( 8 ) and are being pursued as a treatment approach for patients with cancer. (snmjournals.org)
The presence of neoplastic islands, classified as large or small according to the number of cells in the ITF, has been described as the most aggressive pattern compared to tumors with a more uniform growth pattern, as tumor invasion occurs in a more widespread manner as cellular islands or single cells 4 . (nature.com)
This is supported by studies in knockout mice showing development of soft-tissue sarcomas, ovarian stromal cell tumors and a high sensitivity to carcinogenic treatments. (cancerindex.org)
Cell adhesion molecule-1 (CADM1) is a member of the immunoglobulin superfamily that functions as a tumor suppressor of lung tumors. (cancerindex.org)
[ 2 ] Targeted disruption of TP53 in the mouse leads to the development of various tumors (see image below). (medscape.com)
Tumors that developed in mice deficient in INK4A were enhanced by the topical application of carcinogens and ultraviolet light. (medscape.com)

first available as pre-print ) van Boxtel AL (2023), 'Whole-Mount In Situ Hybridization for Detection of Migrating Zebrafish Endodermal Cells' , Methods Mol Biol. (vanamerongenlab.nl)
Our research includes the use of knockout mice and zebrafish as laboratory models. (ncl.ac.uk)

The p16(Ink4a) and p19(Arf) knockout mice were generated via transcription activator-like effector nucleases (TALENs), and p27(Kip1) knockout mice via clustered regularly interspaced short palindromic repeats/CRISPR-associated nuclease 9 (CRISPR/Cas9). (koreamed.org)
Direct evidence linking the INK4A locus to tumorigenesis was provided by the targeted disruption of exon 2 of INK4A in mice. (medscape.com)

and cell proliferation, and commit to cell cycle arrest because of AP-1 repression. (wikipedia.org)
JDP2 induces cell cycle arrest through cyclin D, p53, and cyclin A transcription, by increasing JUNB, JUND, and Fra2, and by decreasing c-JUN through the loss of p27kip1. (wikipedia.org)
The engagement of CTLA4 by costimulatory molecules results in decreased T-cell receptor signaling, interleukin 2 transcription ( 3 ), and cell cycle arrest at the G1 stage, with the final result of inducing T-cell anergy ( 4 , 5 ). (snmjournals.org)
Arrest in G2 phase of the cell cycle was confirmed by positive Fursultiamine staining with cyclin B1 antibody (Fig? (antiviralbiologic.com)
Activation of p21 or p16 therefore causes cell cycle arrest. (medscape.com)

The number of PCNA (proliferating cell nuclear antigen) positive cells was higher in ahnak knock-out mice than wild type mice at 24, 48 and 72hrs after hepatectomy. (snu.ac.kr)

germline knockout mice possess impaired physical and hair follicle developmental kinetics most readily observed within the pre-weaning period [14 15 On select genetic backgrounds these mice develop piebaldism (suggestive of defective melanocyte function) and symptoms analogous to Type II Waardenburg syndrome (characterized by hearing loss and skin/hair pigment anomalies) [16]. (bioinf.org)

Mutations in cyclin-dependent kinase inhibitors controlling the G1 phase of the cell cycle are prevalent in various cancers. (koreamed.org)
Here, we generated knockout mouse models for each of these three cyclin-dependent kinase inhibitors using engineered nucleases. (koreamed.org)
Microglial dyshomeostasis drives perineuronal net and synaptic loss in a CSF1R+/- mouse model of ALSP, which can be rescued via CSF1R inhibitors. (stanford.edu)

C and D) Representative results of PCR genotyping for FVB (C) and B6 (D) strains of WT and p19Arf KO mice. (koreamed.org)

Therefore, the liver regeneration was examined in ahnak knock-out mice after 70% partial hepatectomy. (snu.ac.kr)
It was significantly increased in ahnak knock-out mice at 48 hrs after hepatectomy. (snu.ac.kr)
Comparing with ahnak wild type mice, liver mass restoration in ahnak knock-out mice was accelerated, which was associated with increased expression levels in immediate early gene c-myc, c-fos, c-jun. (snu.ac.kr)
Using HSL knock out mice, we were able to demonstrate that the lipase controls insulin secretion. (lu.se)

Pharmacological inhibition, genetic silencing or knockout of Plk1 attenuated obstructive nephropathy due to suppressed fibroblast activation mediated by reduced autophagic flux. (nature.com)
Importantly, Wnt signaling inhibition in Fzd9-competent mice largely recapitulates the suppression of proliferation caused by Fzd9 deficiency upon Myc activation. (life-science-alliance.org)

Using a mouse model of MWCNT exposure, we found that the tissue inhibitor of metalloproteinase 1 (Timp1) gene was rapidly and highly induced in the lungs by MWCNTs in a time- and dose-dependent manner. (cdc.gov)
As microglial elimination is unlikely to be clinically feasible for extended durations, we treated adult CSF1R+/- mice at different disease stages with a microglia-modulating dose of the CSF1R inhibitor PLX5622, which prevented microglial dyshomeostasis along with synaptic- and ECM-related deficits. (stanford.edu)
The p16INK4A protein is a cell-cycle inhibitor that acts by inhibiting activated cyclin D:CDK4/6 complexes, which play a crucial role in the control of the cell cycle by phosphorylating Rb protein. (medscape.com)

In particular, ubiquitin-mediated degradation is critically important at transition points where it provides directionality and irreversibility to the cell cycle, which is essential for maintaining genome integrity. (intechopen.com)
He serves on the Editorial Boards of Annual Review of Cell and Developmental Biology and Genome Biology. (elifesciences.org)
Mitochondrial DNA (mtDNA) is a critical genome contained within the mitochondria of eukaryotic cells, with many copies present in each mitochondrion. (bvsalud.org)
studies on cell cycle regulation and genome instability. (ncl.ac.uk)

The gene-targeting construct was launched into embryonal stem (ES) cells and heterozygous cyclin A2f/+ (Af denotes XI-006 the "floxed" allele) ES were obtained through homologous recombination (Figures 1A and 1B). (immune-source.com)

The latter represents a conformationally modified form of a normal cellular PrP C , which is a normal host protein found on the surface of many cells, in particular neurons. (medscape.com)

we show that mig-6 is a negative regulator of EGFR signaling in mouse hepatocytes in vivo. (jnksignaling.com)
After a 70% PH, mig-6 knockout mice display an increase in hepatocytes re-entering the cell cycle at early time points during liver regeneration, which correlated with enhanced EGFR signaling. (jnksignaling.com)
point, suggesting that mig-6 knockout hepatocytes are in a primed state. (jnksignaling.com)
Therefore, it seems plausible that mig-6 knockout hepatocytes are able to re-enter the cell cycle faster than their wild-type counterparts. (jnksignaling.com)
Complete loss of PR-SET7 in the hepatocytes of these mice (designated is deleted in our model) and P45 is less than one (Supplementary Fig S2A), the above finding suggests that H4K20Me1 is a relatively stable modification, which is preserved in non-dividing cells, even in the absence of PR-SET7. (antiviralbiologic.com)
All of?the large cells in Area-B and the smaller cells in Area-C were HNF4-positive hepatocytes (Fig? (antiviralbiologic.com)
Open in a separate window Figure 2 Postnatal inactivation of PR-SET7 in hepatocytes leads to cell death A Macroscopic appearance of livers in 120-day-old (P120) wild-type (WT) and (KO) mice. (antiviralbiologic.com)

Furthermore, we were able to show that negative regulators of the cell cycle like retinoblastoma are inactivated in regenerating mig-6 knockout livers. (jnksignaling.com)

Recent research in the lab indicates that inflammation promotes cellular iron overload in a vicious cycle leading to cell death. (upenn.edu)
On the other hand, it has been reported that compared to RAGE-knockout animals, wild type animals are more susceptible to these AGE-associated diseases, including acute inflammation and cancers caused by exogenous stimuli [14]. (heraldopenaccess.us)
Our results demonstrate that in these mice, hepatocyte death initially leads to the activation of ductal progenitors and inflammation, followed by spontaneous development of hepatocellular carcinoma comprised mainly of cells featuring cancer stem cell properties. (antiviralbiologic.com)
Recent findings show that encountered in the workplace include toluene diisocya- sensitized mice develop airway hyperreactivity and inflammation, nate (TDI), diphenyl-methane diisocyanate and hexam- reflective of human disease. (cdc.gov)

Low serum albumin level before rituximab and high CD56+CD16+ natural killer cell count after rituximab were independent risk factors of relapse within two years after rituximab treatment. (bvsalud.org)
This clinical state is characterized by elevated basal serum FSH levels in association with disordered menstrual cycles as demonstrated by oligomenorrhea, polymenorrhea, or metrorrhagia. (medscape.com)
The transfer of lymphocytes or ethylene di-isocyanate (HDI), which are used in a serum from sensitized mice can cause clinical disease in naive variety of industries including polyurethane foam mice. (cdc.gov)

The encoded protein also interacts with a complex of other cell cycle regulators to repress cell cycle-dependent gene expression in non-dividing cells. (utsouthwestern.edu)
Here, we describe how only the lack of the receptor Fzd9, previously identified as a Myc transcriptional target, impairs sustained tumor expansion and β-cell dedifferentiation in a mouse model of Myc-driven insulinoma, allows pancreatic islets to maintain their physiological structure and affects Myc-related global gene expression. (life-science-alliance.org)

Numerous E3 ubiquitin ligases, which facilitate the ubiquitination of specific substrates, have been shown to control G1/S. In this chapter, we will discuss components in the ubiquitin proteasome system that are implicated in G1/S control, how these enzymes are interconnected, gaps in our current knowledge, and the potential role of these pathways in the cancer cycle and disease proliferation. (intechopen.com)
Cell cycle signaling pathways associated with ahnak may provide new insights for improved understanding of liver regeneration. (snu.ac.kr)
In line with this, the levels of activated EGFR signaling drop at 48 hours and are comparable in knockout and wild-type mice, suggesting that the EGFR is inactivated through a mig-6-independent mechanism and, that other pathways, like the MET receptor pathway are induced and drive hepatocyte proliferation. (jnksignaling.com)

Notably, the G1/S boundary represents a major barrier to cell proliferation and is universally dysfunctional in cancer cells, allowing for the unbridled proliferation observed in malignancy. (intechopen.com)
Oral squamous cell carcinoma (OSCC) is the most common type of head and neck malignant tumor and is ranked the eighth leading cause of cancer worldwide. (nature.com)
Cell cycle dysfunction can cause severe diseases, including neurodegenerative disease and cancer. (koreamed.org)
Due to its key role in the eukaryotic cell cycles, Plk1 has been one of the most validated drug targets for cancer treatment. (nature.com)
Ahnak is a 700kDa giant protein that is initially identified in human neuroblastoma and other cancer cells. (snu.ac.kr)
Even though, there have been several papers for elucidating the biological role of ahnak involved in cell cycle and cancer cells. (snu.ac.kr)
Using a combination of chromatin biochemistry, computational modelling, atomic force microscopy (AFM), genetics, genomics and cell biology, Dr. Dalal and colleagues are investigating whether chromatin adopts alternate structural conformations in cancer cells, the functional consequences of large-scale chromosomal alterations upon the cancer epigenome, and identifying small molecules which can target these structures or processes. (elifesciences.org)
It should be clear because of these links that just a simple mismatch in circadian cycles can lead to the development of cancer and neolithic disease. (jackkruse.com)
How does cancer occur when the circadian cycles are off? (jackkruse.com)

When temperature becomes the dominant environmental trigger and not light cycles, leptin induces endothelial nitric oxide synthetase (eNOS) that shuts down the photic effects of VIP on the SCN. (jackkruse.com)

Here, we report that the central proinflammatory cytokine TNF-α acts as a diffusible mediator of the bystander effects induced by macrophages, an effect caused by a proliferation of macrophages that trigger epithelial cell production of Netrin-1, a neuronal guidance molecule. (aacrjournals.org)
Moreover, in Znf179 knockdown cells, p35, a neuronal-specific Cdk5 activator, which is known to activate Cdk5 to phosphorylate and stabilize p27, also decreased. (tmu.edu.tw)

Rnd3 is a small Rho-GTPase involved in the regulation of cell-cycle distribution, cell migration, and cell morphogenesis. (thno.org)
In addition, myocardial transcription factors, non-coding RNAs, cyclins, and cell cycle-dependent kinases are involved in the multimodal regulation of pre-existing cardiomyocyte proliferation. (bvsalud.org)
We examined regulation of lipid partioning in beta-cells and its control of insulin secretion. (lu.se)

Causal impact of a full-gene deletion will be investigated in all organ systems by comparing phenotypic characteristics of KO-mice with wild-type control mice of the same genetic C57BL/6N background (Munoz-Fuentes et al. (springer.com)

Blue cells = expressed in wild-type. (jax.org)
A schematic of the Cdkn1b locus with single guide RNA target nucleotides (blue) and the amino acid sequences (purple) of the wild-type (upper) and knockout (lower) mice are shown. (koreamed.org)
2003 Cyclin A2f/f mice were viable and phenotypically normal (data not shown) consistent with our expectation that this "floxed" cyclin A2 allele is usually functionally wild-type. (immune-source.com)
Tolmetin In line with this interpretation, HB-EGF expression is comparable between mig-6 knockout and wild-type mice at 48 hours after PH similar to EGFR activation levels. (jnksignaling.com)
B Representative hematoxylin and eosin staining of liver sections from P120 wild-type (WT) and mice. (antiviralbiologic.com)

The protein encoded by this gene is a putative serine/threonine kinase that localizes to the mitotic apparatus and complexes with cell cycle controller CDC2 kinase in early mitosis. (cancerindex.org)
1992 In addition cyclin A2 was postulated to play a role in access of cells into mitosis (Swenson et al. (immune-source.com)
Cell division is called mitosis. (jackkruse.com)
Mitosis is the phase in the cell that occurs just before cell division to generate an offspring. (jackkruse.com)
Homozygous deletion in adult mice causes premature death, intestinal epithelium atrophy, and abnormal mitosis. (utsouthwestern.edu)

Cyclin A the first cyclin ever cloned is regarded as an essential component of the cell cycle engine. (immune-source.com)
Cyclin A2f/+ ES cells were then injected into mouse blastocysts and homozygous cyclin A2f/f animals were generated using standard procedures (Geng et al. (immune-source.com)
Physique 1 Generation of Cyclin A2f/f Mice In order to verify that deletion of the "floxed" cyclin A2 sequences resulted in a functionally null allele we crossed cyclin A2f/f mice with a "deleter" Meox2-Cre strain (Tallquist and Soriano 2000 and generated cyclin A2? (immune-source.com)
2005 and generated cyclin A1-/-A2f/f mice. (immune-source.com)

Once a T cell is activated through its T-cell receptor, downstream T-cell receptor signaling through Src kinases results in tyrosine phosphorylation of CTLA4 and the uncoupling of CTLA4 from AP50, allowing its surface expression, which peaks at 48 h after activation. (snmjournals.org)
The protein is phosphorylated in a cell-cycle dependent manner, with late prophase phosphorylation remaining through metaphase. (cancerindex.org)

Mice homozygous for a gene-trap allele die shortly after implantation with defects in early embryogenesis. (utsouthwestern.edu)

These extensive high-throughput data from IMPC mice provide a promising opportunity to explore genetics causing metabolic heart disease with an important translational approach. (springer.com)
The epic battle for the cell is to have the regularly expected circadian cycles found in our environment and "yoke" those signals to its metabolic cycle and to its growth cycle. (jackkruse.com)
This was made possible by a metabolomics approach, using NMR to map metabolic pathway activity in cells. (lu.se)

In CKD patients or obstructive mice model, we observed that Plk1, a critical mitotic regulator, was upregulated in kidneys. (nature.com)

During a low redox time cells are usually recycling their components using autophagy during sleep. (jackkruse.com)

3) Use FACS to isolate single retinal cell types and determine the effects of iron transporter knockouts on their transcriptome. (upenn.edu)

A mouse line deficient in the iron transporting ferroxidases ceruloplasmin and hephaestin develops age-dependent retinal iron overload and retinal degeneration with features of AMD (Hahn et al. (upenn.edu)
Unlike both and [19 20 Our studies however additionally analyzed the loss of Snai3 in the context of a Snai2 deficient animal a germ line double knockout (DKO) which resulted in clear developmental abnormalities LY2603618 (IC-83) [20]. (bioinf.org)

C and D) Representative results of PCR genotyping for FVB (C) and C57BL/6 (D) strains of WT and p16Ink4a KO mice. (koreamed.org)

The few residual hepatocyte-like cells had a more eosinophilic appearance and enlarged nuclei with sponge-like condensation of chromatin (Fig? (antiviralbiologic.com)

In contrast to drugs, the administration of sRAGE into animal models generated for AGE-related diseases has been shown to obstruct the cellular AGE-RAGE interaction by competing with RAGE and contribute to disease suppression and precluding cell death [16]. (heraldopenaccess.us)

As a transcription factor whose expression is increased by DNA damage, p53 blocks cell division at the G1 phase of the cell cycle to allow DNA repair. (medscape.com)

We recently found low level of tumor suppressor programmed cell death 4 (PDCD4) associated with reduced atherosclerotic plaque area (unpublished). (plos.org)
Programmed cell death 4 (PDCD4) is an important tumor suppressor in the development of various human cancers [1] and inhibits translation rather than transcription. (plos.org)

Preclinical models predict that blockade of the coinhibitory molecule cytotoxic T lymphocyte-associated antigen 4 (CTLA4) on lymphocytes results in the release of a cell cycle inhibitory checkpoint, allowing lymphocyte proliferation, tumor targeting, and regression. (snmjournals.org)

Recently, gene-editing techniques, including programmable nucleases such as restriction endonuclease, zinc finger nuclease, transcription activator-like effector nuclease, clustered regularly interspaced short palindromic repeats/clustered regularly interspaced short palindromic repeats-associated 9 and base editors, have provided new tools for editing mtDNA in mammalian cells. (bvsalud.org)
In the most well characterized "canonical" pathway in mammalian cells, the Notch intracellular domain (NotchIC) translocates to the nucleus where it mostly associates with the recombination signal binding protein for the immunoglobulin kappa J region (RBPJκ). (molvis.org)

1. Artelt P, Grannemann R, Stocking C, Friel J, Bartsch J, Hauser H. The prokaryotic neomycin-resistance-encoding gene acts as a transcriptional silencer in eukaryotic cells. (koreamed.org)

We will use the knockout mice to investigate the physiological role of Znf179. (tmu.edu.tw)

We showed how insulin deficiency in Friedreich's ataxia is likely to evolve, and that a mouse model for Huntington's disease develops diabetes due to loss of beta-cells. (lu.se)

In the mammalian heart, cardiomyocytes are forced to withdraw from the cell cycle shortly after birth, limiting the ability of the heart to regenerate and repair. (bvsalud.org)

Mouse p53 protein negatively regulates the JDP2 promoter in F9 cells as part of the JDP2˗p53 autoregulatory circuit. (wikipedia.org)

Most importantly, we described the critical role of pyruvate cycling in insulin secretion and how it underlies stimulus-secretion coupling in the beta-cell. (lu.se)

Figure 1 TALEN-mediated generation of a p16Ink4a knockout mouse model. (koreamed.org)
Figure 3 CRISPR/Cas9-mediated generation of B6-p27Kip1 knockout mouse model. (koreamed.org)
In rodent model, the rapid restoration of the liver mass provides a model for studying cell cycle. (snu.ac.kr)
1997 These studies have led to the current model that this "core" components of the cell cycle machinery (cyclins A and B) symbolize XI-006 absolutely essential elements of the cell cycle engine (Hochegger et al. (immune-source.com)
Yet, the mouse shares a similar developmental trajectory to the human heart and a comparable four-chamber morphology that greatly enhances its translational value as a model organism, despite the substantial differences in size (Krishnan et al. (springer.com)

Kappe's mutation analyses on TRAP showed that it underlies the unusual gliding motility that malaria parasites use to move into host cells. (the-scientist.com)
Flow cytometric analyses revealed that the population of G0/G1 cells decreased in Znf179 knockdown cells. (tmu.edu.tw)
We have acquired several advanced mass spectrometers over the years that permitted analyses of the metabolome in cell extracts and body fluids. (lu.se)

A and B) Representative views of the transcription activator-like effector nuclease (TALEN) targeting strategy for generation of p16Ink4a KO mice in both FVB (A) and B6 (B) strains. (koreamed.org)

Anatomy29

Organisms21

Diseases5

Chemicals and Drugs88

Analytical, Diagnostic and Therapeutic Techniques and Equipment27

Psychiatry and Psychology2

Phenomena and Processes89

Disciplines and Occupations1

Information Science3

Health Care2

Transgenic4

Differentiation17

Genes14

Apoptosis8

Proteins3

Leads to cell cycle arrest2

Progression7

Conditional6

Progenitor1

Embryonic8

Fibroblasts5

Metabolism8

Phenotype3

Vitro3

Epithelial cells3

Stem cells9

Tumors6

Zebrafish2

Ink4a2

Arrest5

Proliferating cell nu1

Germline1

Inhibitors3

P19Arf1

Knock-out4

Inhibition2

Inhibitor3

Genome4

Heterozygous1

Neurons1

Hepatocytes7

Livers1

Inflammation4

Serum3

Gene expression2

Pathways3

Cancer9

Induces1

Neuronal2

Regulation3

Genetic1

Wild-type5

Mitosis5

Cyclin4

Phosphorylation2

Homozygous1

Metabolic3

Mitotic1

Autophagy1

Transporter1

Deficient2

C57BL1

Chromatin1

Cellular1

Transcription factor1

Tumor suppressor2

Models1

Mammalian cells2

Eukaryotic cells1

Physiological1

Deficiency1

Shortly1

Regulates1

Secretion1

Model5

Analyses3

Effector1