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  • siRNA
  • siRNA-mediated knockdown study showed that the effects of SFN on LPS-induced reactive oxygen species, reactive nitrogen species, and pro-inflammatory cytokine production and cell death are partly Nrf2 dependent. (frontiersin.org)
  • A small interfering RNA (siRNA) directed against the loop region of the miR369-3 precursor (si-pre369) ( Fig. 1B and fig. S2) prevented translation (knocked down) up-regulation under serum-starved conditions. (sciencemag.org)
  • When knockdown was followed by rescue with synthetic miR369-3 resistant to the siRNA ( Fig. 1C ), translation efficiency was increased fivefold. (sciencemag.org)
  • mRNAs
  • Computational analysis showed the presence of miR-93 consensus sequences in the 3′UTR region of both VEGF and IL-8 mRNAs, predicting possible interaction with miR-93 and suggesting a potential regulatory role of this microRNA. (biomedcentral.com)
  • MicroRNAs that are perfectly matched with their target mRNAs (often in 3ʹ-UTR) can degrade their targets, but those which are matched with their targets imperfectly inhibit their translation into the protein. (openaccesspub.org)
  • MicroRNAs are short RNAs that bind to the 3' UTR of target mRNAs to repress their translation and accelerate their decay. (biomedcentral.com)
  • sequences
  • MicroRNA sequences: wild-type miR369-3, seed mutant miRseedmtR369-3 (complementary to mtAREseed1 and mtAREseed2), and miRmt369-3 (complementary to mtARE). (sciencemag.org)
  • Bioinformatic
  • A bioinformatic screen identified five microRNAs in miRBASE with seed regions complementary to the TNFα ARE, not including miR16 ( 5 )[see supporting online material (SOM) text]. (sciencemag.org)
  • thereby
  • Second, can micro-ribonucleoproteins (microRNPs), in general, up-regulate translation under growth-arrest conditions, thereby switching between repressing and activating roles in response to the cell cycle? (sciencemag.org)
  • assay
  • In the present study, qRT-PCR assay revealed that the expression of miR-31 was upregulated in human cervical cancer cells and clinical tissues. (hindawi.com)
  • Results of wound healing and cell migration assay revealed that knockdown of miR-31 inhibited cell metastasis and migration. (hindawi.com)
  • MicroRNA profile assay successfully detected a branch of differential expression miRNAs between PTC and normal tissue. (medsci.org)
  • transition
  • HDAC1 knockdown in tumor cells also impairs the G 2 /M transition and inhibits cell growth, as evidenced by a reduction of mitotic cells and an increased percentage of apoptotic cells ( 13 ). (asm.org)
  • The aim of this study was to validate the potential role of miR-31 and BRCA1-associated protein-1 (BAP1) on regulating epithelial-mesenchymal transition (EMT) in cervical cancer. (hindawi.com)
  • tissue
  • Endothelial cell tumors are the most common soft tissue tumors in infants, yet little is known about the significance of miR in regulating their growth. (pubmedcentralcanada.ca)
  • Microarray is a powerful technique of assessment of expression of genes in a tissue at any time at genomewide scale and has been applied in understanding role of adipose tissue in insulin resistance [ 28 - 31 ]. (hindawi.com)
  • The expression of miRNA is significantly differential between tumor and normal tissue, in addition to among various tumor tissues [ 8 - 9 ], suggesting the critical role of miRNAs in tumor genesis [ 10 ]. (medsci.org)
  • pathway
  • Furthermore, XTbx6 activates the expression of Xwnt8 and FGF8, which are two mediators of posterior development, suggesting a mechanism by which XTbx6 modulates posterior patterning via Wnt and FGF signalling pathway activation. (labome.org)
  • Taken together, our study suggests that GLP-1 promotes the novel insulin-mimetic adipocytokine visfatin expression via the PKA pathway and might influence glucose metabolism. (labome.org)
  • cell cycle
  • Loss of HDAC1 and -2 induces expression of these cyclin-dependent kinase (CDK) inhibitors, leading to a cell cycle block in G 1 . (asm.org)
  • embryonic
  • In 1993, the first miRNA, lin-4, was discovered as a 22-nucleotide non-coding RNA that regulated the timing of post-embryonic development by repressing lin-14 protein expression in Caenorhabditis elegans [ 1 ]. (mdpi.com)
  • We recently reported the cloning and expression of bovine NOBOX during early embryonic development and our gene knockdown studies indicate that NOBOX is a maternal effect gene essential for early embryonic development. (biomedcentral.com)
  • Expression analysis of miR-196a in bovine oocytes and during early embryonic development indicated that it is expressed both in oocytes and embryos and tends to increase at the four-cell and eight-cell stages. (biomedcentral.com)
  • Aberrant
  • BIRC5 (also known as survivin) is the most important member because of its widely aberrant expression in most types of cancers according to the National Cancer Institute (NCI) cancer drug-screening program [ 18 ]. (jcancer.org)
  • maternal
  • Moreover, the placenta functions as an endocrine organ producing a number of pregnancy-associated hormones and growth factors to regulate fetal growth and maternal physiology [ 4 , 5 ]. (mdpi.com)
  • Oocyte-derived maternal RNAs drive early embryogenesis when the newly formed embryo is transcriptionally inactive. (biomedcentral.com)
  • short
  • Additional bioinformatics analysis of short RNA sequencing data from ENCODE cell lines showed that miR-1285 is expressed in different cancer cell lines and differentiated cells. (openaccesspub.org)