Knock out the gene. Medical search. Frequent questions

Mutations in the human p53 gene, for example, can be induced by exposure to benzo(a)pyrene (BaP) and the mutated copy of the p53 gene can be inserted into mouse genomes. (wikipedia.org)
In addition, HR donors can include up to 6-8 kb of open reading frame for gene knock-ins or tagging, and, when small mutations are included in either 5' or 3' homology arms, can make specific, targeted gene edits. (reportergene.com)
Mutations in the tumor suppressor genes encoding TSC1 (Hamartin) and TSC2 (Tuberin) cause a multisystemic tumor syndrome termed tuberous sclerosis complex (TSC). (springer.com)
At the same time, two silent mutations were also introduced into the gene to allow genotyping by restriction enzyme digestion. (alzforum.org)
Between 40 to 50 percent of cases of retinitis pigmentosa have no explanation, meaning they carry mutations in genes yet to be identified. (eurekalert.org)
If we find cases with mutations in this specific gene, or on any retinal microexons, it could lead to potential new therapeutic strategies to manage the condition," says Ludovica Ciampi , PhD student at the CRG and first author of the study. (eurekalert.org)
To maximize the potential disease relevance, genes with a genome-wide significant excess burden of loss-of-function mutations in NPDs such as intellectual disability/developmental delay, autism spectrum disorder, and schizophrenia are the focus. (nih.gov)
Mutations in the MAN2B1 gene cause alpha-mannosidosis. (medlineplus.gov)
Mutations in the MAN2B1 gene interfere with the ability of the alpha-mannosidase enzyme to perform its role in breaking down mannose-containing oligosaccharides. (medlineplus.gov)
which means both copies of the gene in each cell have mutations. (medlineplus.gov)
Congenital Hypophosphatasia Congenital hypophosphatasia is absence or low levels of serum alkaline phosphatase due to mutations in the gene encoding tissue-nonspecific alkaline phosphatase (TNSALP). (msdmanuals.com)
Candidate genes for cblA, cblB, designated MMAA and MMAB, and mutations of these genes have been elucidated. (medscape.com)
If this is the case, gene-correction presents a solution for patients with known monogenetic mutations. (lu.se)

More recent developments in knock-in technique have allowed for pigs to have a gene for green fluorescent protein inserted with a CRISPR/Cas9 system, which allows for much more accurate and successful gene insertions. (wikipedia.org)
The speed of CRISPR/Cas9-mediated gene knock-in also allows for biallelic modifications to some genes to be generated and the phenotype in mice observed in a single generation, an unprecedented timeframe. (wikipedia.org)
We used this system for a genome-wide CRISPR knock-out screen, which yielded expected hits (DNMT1, UHRF1, MGA), as well as novel candidates. (pasteur.fr)
Here, we describe CRISPR-Cas9-mediated homology-independent organoid transgenesis (CRISPR-HOT), which enables efficient generation of knock-in human organoids representing different tissues. (nih.gov)
CRISPR-HOT avoids extensive cloning and outperforms homology directed repair (HDR) in achieving precise integration of exogenous DNA sequences into desired loci, without the necessity to inactivate TP53 in untransformed cells, which was previously used to increase HDR-mediated knock-in. (nih.gov)
Target-gene-KO/knock-in (KI) efficiency of CRISPR/Cas9 has not been extensively investigated in marmosets. (elsevierpure.com)
In this study, optimum conditions for target gene modification efficacies of CRISPR/mRNA and CRISPR/nuclease in marmoset embryos were examined. (elsevierpure.com)
Although further improvement of KI strategies is required, these results indicated that CRISPR/Cas9 may be utilized to produce KO/KI marmosets via gene editing. (elsevierpure.com)
TG substitution was introduced into the murine Trem2 gene using CRISPR/Cas9 genome editing. (alzforum.org)
Mar. 22, 2021 Using an improved version of the gene editing tool CRISPR/Cas9, researchers knocked out up to twelve genes in plants in a single blow. (sciencedaily.com)
CRISPR/Cas9 mediated knock-out of VPREB1 gene induces a cytotoxic effect in myeloma cells. (nih.gov)
As part of the trial, researchers used CRISPR-Cas9 to edit specific genes in stem cells-the building blocks of blood cells-taken from each patient. (medicalxpress.com)

Gene knock-in has allowed, for the first time, hypothesis-driven studies on gene modifications and resultant phenotypes. (wikipedia.org)
First, combinations of knock-in genes lead to growing complexity in the interactions that inserted genes and their products have with other sections of the genome and can therefore lead to more side effects and difficult-to-explain phenotypes. (wikipedia.org)
With this we could allude to an intrinsic bias of essentiality across cellular compartments, uncover two opposing roles for tumour suppressor genes and link autosomal-recessive disorders with growth-retardation phenotypes to early embryogenesis. (nature.com)
While it is anticipated that assays should aim to characterize 100-250 genes, some phenotypes and assay formats may be more amenable to moderate scale screening against a subset (50-100) of the gene set. (nih.gov)
Cytochrome P450s were the most overexpressed detoxification genes across all resistant phenotypes. (cdc.gov)

Even though gene knock-outs can result from DSBs caused by Cas9 alone, SBI recommends the use of HR targeting vectors (also called HR donor vectors) for more efficient and precise mutation. (reportergene.com)

Therefore, simultaneous targeting of multiple genes may be necessary. (ejbiotechnology.info)

Lung tumors observed in the knock-in mice offer support for the hypothesis of BaP's carcinogenicity. (wikipedia.org)
Knock-in of sections of the human immunoglobulin gene into mice has already been shown to allow them to produce humanized antibodies that are therapeutically useful. (wikipedia.org)
The biggest disadvantage of using gene knock-in for human disease model generation is that mouse physiology is not identical to that of humans and human orthologs of proteins expressed in mice will often not wholly reflect the role of a gene in human pathology. (wikipedia.org)
The present study explores the possible involvement of a purinergic mechanism in mechanosensory transduction in the bladder using P2X 3 receptor knock-out (P2X 3 − /− ) and wild-type control (P2X 3 +/+ ) mice. (jneurosci.org)
P2X 3 -positive staining was completely absent in the subepithelial plexus of the P2X 3 − /− mice, whereas staining for calcitonin gene-related peptide and vanilloid receptor 1 receptors remained. (jneurosci.org)
Trem2 T66M mice show a gene-dose-dependent accumulation of immature (not fully glycosylated) TREM2 protein in brain. (alzforum.org)
Mice in which the FOG2 gene is knocked out are born with tricuspid atresia. (medscape.com)
Mice with each PON gene knocked out were generated at UCLA and have been key for elucidating their roles in organophosphorus (OP) metabolism, cardiovascular disease, innate immunity, obesity, and cancer. (cdc.gov)

This is the second time within a year that the 18th pick overall out of Arkansas in the 2022 draft has been knocked unconscious on a pass play. (yahoo.com)
He sustained a concussion as a rookie in Philadelphia when knocked out during his lone touchdown grab on Dec. 4, 2022, in a blowout loss to the Eagles. (yahoo.com)
Mar. 1, 2022 Researchers have discovered orf137, the gene underlying male sterility in a strain of tomato plant, CMS tomato. (sciencedaily.com)

Editing a gene using an HR Targeting Vector. (reportergene.com)
For gene editing, this DSB should be within an intron. (reportergene.com)
Gene editing technologies have enabled production of target-gene knock-out (KO) NHP models. (elsevierpure.com)
Other gene therapy studies for SCD have used lentiviruses-a type of virus often modified and used for gene editing which remain in the cell long-term. (medicalxpress.com)
Researchers at the University of Calgary used gene editing technology to develop a shorter, highly branched canola variety with more pods and easier to harvest. (isaaa.org)

Because of this complexity, retinal cells depend on many unique genes for their development, any which of can have a disease-causing mutation and result in vision loss. (eurekalert.org)
In total, 10 unique genes were edited in the donor pig. (dailymail.co.uk)

Interestingly, cuticular-related protein genes (chinase and chitin) were predominantly downregulated, which was also confirmed in the functional enrichment analysis. (cdc.gov)
Interestingly, many genes are induced at the mRNA- and protein level within a few minutes after being touched. (lu.se)

The parents of an individual with an autosomal recessive condition each carry one copy of the mutated gene, but they typically do not show signs and symptoms of the condition. (medlineplus.gov)

Using our recently established haploid human pluripotent stem cells (hPSCs), we generated a genome-wide loss-of-function library targeting 18,166 protein-coding genes to define the essential genes in hPSCs. (nature.com)

Targeting one single gene can lead to the development of escape mutants or may be insufficient to silence redundant pathways. (ejbiotechnology.info)
We show that knock-down of each single gene from multiple target vectors is achieved at an efficiency comparable to that obtained after transduction using single target viral vectors. (ejbiotechnology.info)
One way is to observe how a cell (or a whole organism) behaves when that single gene is absent, or at least not working. (snexplores.org)
The research found that, in vertebrates, Srrm3 works by regulating alternative splicing, a process that allows cells to make more than one type of protein from a single gene and is particularly prominent in neuronal cells. (eurekalert.org)
SSPsyGene is designed to provide a collaborative and efficient framework for identifying biological function beyond current small-scale and often ad hoc single gene efforts in order to generate a standardized, experimentally derived, functional catalog of NPD risk genes. (nih.gov)

The use of loxP flanking sites that become excised upon expression of Cre recombinase with gene vectors is an example of this. (wikipedia.org)

They dynamically regulate the expression of genes, transposable elements, and higher-order chromatin structures. (pasteur.fr)
Our results show that all 6 candidates regulate the expression of germline genes. (pasteur.fr)
The complex is thought to regulate Ig gene rearrangements in the early steps of B-cell differentiation. (nih.gov)
Mouse knock-out strains demonstrated that T2D-associated candidates regulate glucose homeostasis and body composition in vivo. (lu.se)

Yeast-form-specific genes identified in Histoplasma capsulatum function in calcium/calmodulin signaling pathways and sulphur metabolism. (cdc.gov)
This RNA-Seq analysis presents a detailed picture of the candidate detoxification genes and other pathways that are potentially associated with pyrethroid and organophosphate resistance in Ae. (cdc.gov)

In molecular cloning and biology, a gene knock-in (abbreviation: KI) refers to a genetic engineering method that involves the one-for-one substitution of DNA sequence information in a genetic locus or the insertion of sequence information not found within the locus. (wikipedia.org)
Knock-in technology is different from knockout technology in that knockout technology aims to either delete part of the DNA sequence or insert irrelevant DNA sequence information to disrupt the expression of a specific genetic locus. (wikipedia.org)
Gene knock-in technology, on the other hand, alters the genetic locus of interest via a one-for-one substitution of DNA sequence information or by the addition of sequence information that is not found on said genetic locus. (wikipedia.org)
Although specific genetic causes of the malformation remain to be determined in humans, the FOG2 gene may be involved in the process. (medscape.com)
For example, one of the top results in any genetic analysis for heart attack is LDL cholesterol and several genes related to LDL cholesterol. (medscape.com)
We will use genetic tools to knock out genes and study the response of these mutants to touch stimulation in comparison to WT with regards to gene expression, phosphorylation cascades and alteration in growth and development. (lu.se)

Particularly for moderate throughput assays, strategies for selecting a subset of genes from the list should be discussed in the context of the chosen assay(s) or readout(s). (nih.gov)

In this study, recombinant T. cruzi knocking out or overexpressing the TcNTPDase-1 gene were built, and the role of TcNTPDase-1 in the in vitro interaction with VERO cells was investigated. (fiocruz.br)

While gene knock-in technology has proven to be a powerful technique for the generation of models of human disease and insight into proteins in vivo, numerous limitations still exist. (wikipedia.org)
Reporter genes often produce proteins that glow in green or blue. (snexplores.org)
hPSC-enriched essential genes mainly encode transcription factors and proteins related to cell-cycle and DNA-repair, revealing that a quarter of the nuclear factors are essential for normal growth. (nature.com)

or regulatory control sequences, to study effects of temporal changes to GENE EXPRESSION. (harvard.edu)
However, precise integration of exogenous DNA sequences into human organoids is lacking robust knock-in approaches. (nih.gov)

Dec. 5, 2023 Researchers have discovered that electric eels can alter the genes of tiny fish larvae with their electric shock. (sciencedaily.com)

This gene encodes the iota polypeptide chain that is associated with the Ig-mu chain to form a molecular complex which is expressed on the surface of pre-B cells. (nih.gov)

In agreement with GSS activity localization in vivo , we identified six genes encoding arylsulfatase-like enzymes with a predicted C-terminal transmembrane domain, of which five showed GSS activity upon heterologous expression in insect cells. (nature.com)

species of trees other than Eucalyptus have raised the Pheromone MF 1 and MF a 1 gene fragments were poly- strong possibility that this fungus might have broader geo- merase chain reaction-amplified with fluorescently labeled graphic distribution (7-9). (cdc.gov)

To knock out the gene, scientists first find or create a version of the gene that doesn't work. (snexplores.org)

Researchers at The University of Queensland (UQ) have developed a technology for targeted and stable integration of a gene of interest in a manner that affords control of copy number and - when tested on a range of target genes in S. cerevisiae - increased protein expression compared to standard yeast systems. (uniquest.com.au)
Researchers can insert new genes into these or knock out existing genes with great precision. (newscientist.com)
Researchers at the Centre for Genomic Regulation (CRG) in Barcelona reveal that Srrm3 is a master regulator gene crucial for the development of photoreceptors, cells in the back of the retina which capture and process light, sending signals to the brain that enable vision. (eurekalert.org)

The gene ontology (GO) terms associated with monooxygenase, iron binding, and passive transmembrane transporter activities were significantly enriched in four out of six resistant vs. susceptible comparisons while serine protease activity was elevated in all insecticide-resistant groups relative to the susceptible strain. (cdc.gov)

It should be possible to modify stem cells in humans to restore targeted gene function in certain tissues, for example possibly correcting the mutant gamma-chain gene of the IL-2 receptor in hematopoietic stem cells to restore lymphocyte development in people with X-linked severe combined immunodeficiency. (wikipedia.org)
Pigs have a gene that produces a molecule not found in humans that triggers an immediate and aggressive immune response in humans, called hyperacute rejection. (dailymail.co.uk)

Kallikrein gene 'knock-down' by small interfering RNA transfection induces a profibriotic phenotype in rat mesangial cells. (le.ac.uk)

RNA interference (RNAi), mediated by short double-stranded RNAs, is a powerful mechanism for posttranscriptional gene silencing. (ejbiotechnology.info)
The aim of the current investigation was to delineate the role of kallikrein in the regulation of fibrosis, by 'knocking down' its expression using specific small interfering RNAs (siRNA). (le.ac.uk)

The protein encoded by this gene belongs to the immunoglobulin superfamily and is expressed selectively at the early stages of B cell development, namely, in proB and early preB cells. (nih.gov)

New research published in the New England Journal of Medicine indicates that stem cell gene therapy may offer a promising, curative treatment for the painful, inherited blood disorder sickle cell disease (SCD). (medicalxpress.com)
The University of Chicago Medicine Comer Children's Hospital was one of three sites to enroll patients in the clinical trial, which tested a stem cell gene therapy to treat sickle cell disease. (medicalxpress.com)
Theories of drug development, including identification of genes for diagnostics, protein and drug interactions, models for 'knock-out' and 'knock-down' of genes, gene therapy, cancer and stem cell biology, as well as clinical assessment. (lu.se)

It is the opposite of gene knockout. (wikipedia.org)
Gene knock-in originated as a slight modification of the original knockout technique developed by Martin Evans, Oliver Smithies, and Mario Capecchi. (wikipedia.org)
A gene knock-in therefore can be seen as a gain-of-function mutation and a gene knockout a loss-of-function mutation, but a gene knock-in may also involve the substitution of a functional gene locus for a mutant phenotype that results in some loss of function. (wikipedia.org)
Scientists can use that cellular toolbox to slip their knockout gene into the DNA. (snexplores.org)
Results show that epimastigote forms of hemi-knockout parasites showed about 50% lower level of TcNTPDase-1 gene expression when compared to the wild type, while the T. cruzi overexpressing this gene reach 20 times higher gene expression. (fiocruz.br)
In trypomastigote forms, the same decreasing in TcNTPDase-1 gene expression was observed to the hemi-knockout parasites. (fiocruz.br)

Conversely, suppressing PA degradation by inhibiting LPP activity using any of three different inhibitors significantly stimulated pollen tube growth, and similar effect was achieved by suppressing the expression of tobacco pollen LPP4 using antisense knock-down. (frontiersin.org)
We developed antisense oligonucleotides that suppressed AR gene expression in the periphery but not the CNS after subcutaneous administration. (nih.gov)

gene expression and secretion. (lu.se)

At UChicago Medicine, we've built infrastructure to support new approaches to sickle cell disease treatment and to bring additional gene therapies for other diseases. (medicalxpress.com)
Two other gene therapies for the disease are awaiting FDA approval this year. (medicalxpress.com)
The data from this trial supports bringing on similar gene therapies for sickle cell disease and for other bone marrow-derived diseases. (medicalxpress.com)
Arrowhead's therapies use the RNA interference mechanism to quickly and durably knock down certain target genes. (nasdaq.com)

Taken together, the partial silencing and overexpression of the TcNTPDase-1 gene generated viable parasites with low and high infectivity rates, respectively, corroborating that the enzyme encoded for this gene plays an important role to the T. cruzi infectivity. (fiocruz.br)
Overexpression of a gene by increasing its copy number is generally desirable, but that copy number (and therefore yield) is often traded for other important factors such as growth efficiency and/or unstable modifications. (uniquest.com.au)

One additional gene in the pig was knocked out to prevent excessive growth of the pig heart tissue. (dailymail.co.uk)

The ability to inhibit two or more genes simultaneously from one single expression vector further widens the application spectrum of RNAi, both in functional studies and therapeutic strategies. (ejbiotechnology.info)

Each human cell contains some 20,500 different genes. (snexplores.org)
They insert this unworking gene into the DNA of a healthy cell. (snexplores.org)
Called a reporter gene , that tag can alert scientists when the new gene has been successfully accepted by the cell. (snexplores.org)
When scientists insert their dud gene into a cell, they make sure it has perfect copies of the DNA surrounding the gene that they want to replace. (snexplores.org)
Fig. 2: Analysis of cell-essential genes. (nature.com)
To overcome the high cost of processing single-cell RNA sequencing, multiplexing methods have emerged that accelerate knowledge gain in gene and drug discovery. (uniquest.com.au)
A large proportion of these microexons affect the function of around 70 genes important for the development of a photoreceptor's outer segment, the part of the cell that absorbs light. (eurekalert.org)
Mutational Analysis of the VPREB1 Gene of Pre-BCR Complex in a Cohort of Sporadic Pediatric Patients With B-Cell Acute Lymphoblastic Leukemia. (nih.gov)
The findings from a new clinical trial, published August 31, add to the body of evidence supporting gene therapy as a treatment for sickle cell disease, which primarily impacts people of color. (medicalxpress.com)
There's been a great deal of effort towards offering patients different types of transplants with decreased toxicities, and now gene therapy rounds out the set of available treatments, so every patient with sickle cell disease can get some sort of curative therapy if needed. (medicalxpress.com)
As the scientific community continues to refine and expand the applications of gene therapy, the potential for curative treatments for diseases like sickle cell disease is becoming more of a transformative reality. (medicalxpress.com)
In the larger context of therapeutic development, LaBelle stressed the importance of the study's contribution to the growing body of evidence supporting the viability of gene therapy as a treatment for sickle cell disease. (medicalxpress.com)
The fungal cell cyle is considered with respect to the mechanism of sequential gene expression in Candida albicans, since little is known about the cell cycle in pathogenic molds. (cdc.gov)
Genes controlling hyphal development include ones that are upregulated during cell elongation and adherence to epithelia. (cdc.gov)
To track differentiating cell populations, reporter cell lines generated by homologous recombination (knock-in) or via BAC transgenes have been widely used in mouse cells (5-8 ). (lu.se)
Regarding animal models in ASD, many studies focus on gene expression, cortical neuronal migration and cell maturation, and neural network deficits. (bvsalud.org)

Result: The HR event leads to insertion of the region of the HR Donor Vector between the two homology arms-your selection cassette is integrated into the gene, disrupting the open reading frame. (reportergene.com)
If one of the homology arms of the HR donor contains the gene edit, it will be incorporated into the gene through the HR repair process. (reportergene.com)
In Mr Faucette's surgery, three genes were 'knocked out' in the donor pig. (dailymail.co.uk)

Use the PrecisionX™ Gene Knock-out HR Targeting Vector (MCS1-EF1α-RFP-T2A-Puro-pA-MCS2) to knock-out any gene or edit the genome. (reportergene.com)
Knocking-out a gene using an HR Targeting Vector. (reportergene.com)
A targeting vector was designed to insert a cre/ERT2-SV40 polyA cassette followed by an FRT5-flanked pSV40-NeoR-pA cassette into the translational start site of the FBJ osteosarcoma oncogene gene (Fos). (jax.org)

They used molecular scissors to knock out receptors that perceive strigolactone. (isaaa.org)

These results could inform development of novel molecular tools for detection of resistance-associated gene expression in this important arbovirus vector and guide the design and implementation of resistance management strategies. (cdc.gov)

The difference between knock-in technology and traditional transgenic techniques is that a knock-in involves a gene inserted into a specific locus, and is thus a "targeted" insertion. (wikipedia.org)
The goal of this initiative is to fill a very specific knowledge gap area, which is that many protein-coding genes are now associated with NPD risk, but their basic neurobiology remains poorly understood. (nih.gov)
Some detoxification genes (CYP6Z7, CYP28A5, CYP9J2, CYP6Z6, CYP6BB2, CYP6M9, and two CYP9F2 orthologs) were commonly overexpressed in mosquitoes that survived exposure to all three insecticides (independent of geographical origin) while others including CYP6BY1 (malathion), GSTD1 (alpha-cypermethrin), CYP4H29 and GSTE6 (lambda-cyhalothrin) were uniquely overexpressed in mosquitoes that survived exposure to specific insecticides. (cdc.gov)

In summary, our results support distinct functions of Tsc1 and Tsc2 in cellular signaling as the two genes affect ciliary length control and HH signaling via different mechanisms. (springer.com)
The resulting phenotypic data will be integrated across modalities, levels of organization, and genes to create a harmonized, integrated knowledge base needed to substantively advance our understanding of basic gene function and, ultimately, make robust inferences into potential shared and unique disease mechanisms. (nih.gov)

Subsequent offspring of the chimeric mouse will then have the gene knock-in. (wikipedia.org)

Con respecto a los modelos animales en TEA, muchos estudios se centran en la expresión génica, la migración y maduración neuronal cortical y los déficits de la red neuronal, pero muy pocos son los estudios que relacionen directamente la comunicación oral animal y la expresión génica en áreas corticales del lenguaje. (bvsalud.org)

Traditionally, knock-in techniques have relied on homologous recombination to drive targeted gene replacement, although other methods using a transposon-mediated system to insert the target gene have been developed. (wikipedia.org)

Journal Article] Visualization of ecdysteroid activity using a reporter gene in the crustacean, Daphnia. (nii.ac.jp)

This gene provides instructions for making the enzyme alpha-mannosidase. (medlineplus.gov)

Overall, we have constructed an atlas of essential and growth-restricting genes in hPSCs, revealing key aspects of cellular essentiality and providing a reference for future studies on human pluripotency. (nature.com)

Scientists can do that by disabling - or knocking out - a desired gene. (snexplores.org)
Often, however, scientists don't know which gene produces which protein. (snexplores.org)
To find out, scientists can start by knocking out the gene they are interested in. (snexplores.org)
The DNA surrounding the gene the scientists want to replace can act like a disguise and helps sneak the dud gene in. (snexplores.org)
But scientists have not managed to isolate such cells from farm animals, and must rely instead on injecting genes randomly into early embryos. (newscientist.com)

A common use of knock-in technology is for the creation of disease models. (wikipedia.org)

After you've identified clones with your gene-of-interest knocked-out or edited, you can remove the selection cassette using the Cre-LoxP system (learn more about Cre-LoxP excision here). (reportergene.com)

It is a technique by which scientific investigators may study the function of the regulatory machinery (e.g. promoters) that governs the expression of the natural gene being replaced. (wikipedia.org)
Here, we applied Illumina RNA-Seq to study the gene expression patterns associated with resistance to three widely used insecticides (malathion, alphacypermethrin, and lambda-cyhalothrin) in Ae. (cdc.gov)
The object of our study aimed to review and to correlate genes involved in ASD and those related to ultrasonic communication in animal model studies of language-based social behavior at the PubMed database. (bvsalud.org)

These often will be genes with some serious mistake, or mutation . (snexplores.org)

In this way, we were able to knock-down several members of the human Rho-family GTPases in T cells. (ejbiotechnology.info)
The gene-edited pig used in this procedure was provided by Revivicor, a subsidiary of United Therapeutics, one of several biotech companies in the running to develop suitable pig organs for potential human transplant. (dailymail.co.uk)
Six human genes, which are responsible for the immune system accepting the organ, were inserted into the genome. (dailymail.co.uk)
Characterising Mutational Spectra of Carcinogens in the Tumour Suppressor Gene TP53 Using Human TP53 Knock-in (Hupki) Mouse Embryo Fibroblasts. (who.int)
A knock-out mouse model similar to the mut0 human form of methylmalonic acidemia has been developed. (medscape.com)

Our screen also led to the identification of growth-restricting genes whose loss of function provides a growth advantage to hPSCs, highlighting the role of the P53-mTOR pathway in this context. (nature.com)

The Srrm3 gene has neither been associated with the development of photoreceptor cells nor with the pathogenesis of retinal diseases before. (eurekalert.org)

The SSPsyGene Consortium aims to produce a systematic annotation of basic neurobiological function of genes associated with risk for neurodevelopmental and psychiatric disorders (NPDs). (nih.gov)

Anatomy15

Organisms12

Diseases4

Chemicals and Drugs46

Analytical, Diagnostic and Therapeutic Techniques and Equipment24

Phenomena and Processes43

Disciplines and Occupations1

Information Science3

Mutations13

CRISPR12

Phenotypes5

Cas91

Multiple genes1

Mice8

20223

Editing5

Unique genes2

Interestingly2

Autosomal-recessive1

Genome-wide1

Single gene5

Expression of Cre recombinase1

Regulate4

Pathways2

Genetic6

Assays1

Recombinant1

Proteins3

Sequences2

20231

Encodes1

Enzymes1

Fragments1

Version of the gene1

Researchers3

Ontology1

Humans2

Induces1

RNAs2

Immunoglobulin1

Stem cell3

Knockout6

Antisense2

Secretion1

Therapies4

Overexpression2

Additional gene1

Inhibit1

Cell16

Donor3

Targeting3

Receptors1

Molecular1

Specific3

Mechanisms2

Chimeric1

Neuronal1

Target gene1

Reporter gene1

Enzyme1

Cellular1

Scientists5

Disease models1

Clones1

Study3

Mutation1

Human5

Identification1

Diseases1

Disorders1