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Enzyme-Linked Immunosorbent AssayReagent Kits, DiagnosticAntibodies, BacterialImmunoglobulin GSensitivity and SpecificityAntibodies, ViralImmunoglobulin MSerologic TestsCross ReactionsAntigens, BacterialAntibody SpecificityAntibodies, MonoclonalEvaluation Studies as TopicImmunoenzyme TechniquesImmunoglobulin AProto-Oncogene Proteins c-kitAntigens, ViralAntibodies, ProtozoanCattle DiseasesAntibodies, HelminthEpitopesSeroepidemiologic StudiesComplement Fixation TestsAutoantibodiesCattleAntigens, ProtozoanRecombinant ProteinsImmunoassayAntibodiesHemagglutination TestsBlotting, WesternNeutralization TestsRabbitsFecesAntigens, HelminthImmunosorbent TechniquesAgglutination TestsFluorescent Antibody TechniqueBiological MarkersFalse Positive ReactionsImmunoblottingAntibodies, FungalPolymerase Chain ReactionSwine DiseasesCells, CulturedMice, Inbred BALB CMolecular Sequence DataFluorescent Antibody Technique, IndirectReproducibility of ResultsAntigens, FungalHemagglutination Inhibition TestsImmunologic TestsImmunodiffusionHorse DiseasesBrucellaGoatsDog DiseasesRadioimmunoassayLatex Fixation TestsClinical Laboratory TechniquesPredictive Value of TestsBrucellosisCytokinesInterleukin-6Tumor Necrosis Factor-alphaImmune SeraSwineRotavirusLipopolysaccharidesCase-Control StudiesInterleukin-8SerotypingBacterial ToxinsReverse Transcriptase Polymerase Chain ReactionRubella virusPolysaccharides, BacterialSheep DiseasesToxoplasmosisToxoplasmaBacterial VaccinesParatuberculosisArthritis, RheumatoidEnterotoxinsRNA, MessengerTime FactorsCoronaviridae InfectionsGoat DiseasesAmino Acid SequenceNeosporaHorsesAntibody AffinityImmunohistochemistryBacterial Outer Membrane ProteinsRheumatoid FactorCysticercusBacterial ProteinsCoccidiosisAntibody FormationLyme DiseaseSynovial Fluid
AntigenImmunoassayAntibodyELISAsDual-luciferase reporterIntra-assay PrecisionReagentAntibodiesReactivityVitroDiagnosticDetectionReagentsSandwichSpeciesAntigensCommercially availableSensitivityPrecisionSubstrateImmunoassaysIndirectRESULTSDenseLatex agglutinationQuantitative measurementHumanResearchersSamplesHumansReactionMethodAutomationFindingsQuestionnaireProteinsWesternSerumLevelsContentTimeFlowPlasmaTest KitsInfectionMouseDevelopmentPrimary
Antigen7
  • E nzyme-linked immunosorbent assays (ELISA) have been one of the primary methods of detecting the presence of a substance, usually an antigen, in an aqueous sample for more than 40 years. (ddw-online.com)
  • See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more. (lsbio.com)
  • the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. (gentaur.com)
  • Anti-DFS70 autoantibody determination using enzyme-linked immunosorbent assay (ELISA) testing of IgG autoantibodies to human DFS70 antigen was performed by a DFS70 ELISA kit from Medical & Biological Laboratories Co., LTD (7808, Japan) as per manufacturer's instructions. (cdc.gov)
  • Hepatitis B surface antigen assays : operational characteristics (phase 1) : report 2. (who.int)
  • Direct antigen detection kits (ie, rapid antigen tests [RATs], strep screens) have been proposed to allow immediate diagnosis and prompt administration of antibiotics. (medscape.com)
  • Techniques for measuring protein S antigen include enzyme-linked immunosorbent assays (ELISAs) and radioimmunoassays. (medscape.com)
Immunoassay9
  • The immunoassay landscape has, however, dramatically changed in recent years with the development of reduced assay steps protocols, no wash ELISAs and new automated systems designed to run ELISA. (ddw-online.com)
  • The test principle applied in this kit is Sandwich enzyme immunoassay. (avivasysbio.com)
  • Testosterone Saliva ELISA kit is for the quantitative determination of Testosterone by enzyme immunoassay in human saliva. (rapidtest.com)
  • The principle of the following enzyme immunoassay test follows a two-step competitive binding scenario. (rapidtest.com)
  • Learn more about SMC ® technology, immunoassay kits, and how you can design your own custom assay to meet your critical research needs. (sigmaaldrich.com)
  • Providing maximum immunoassay performance while following a workflow similar to traditional ELISA technology, SMC ® technology combines a unique assay elution step and robust digital counting to achieve improved signal-to-noise ratios over traditional immunoassay technologies. (sigmaaldrich.com)
  • Also Enzyme-linked immunoassay, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330. (gentaur.com)
  • An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. (gentaur.com)
  • The Rapid C. Brucella Ab Test Kit is a chromatographic immunoassay for the qualitative detection of Brucella canis antibodies in canine whole blood, plasma or serum. (genomeboy.com)
Antibody4
  • One of such kits, called ELISA (Enzyme-linked immunosorbent assay) uses an antibody that specifically recognizes WMV. (wikipedia.org)
  • The microtiter plate provided in this kit has been pre-coated with an antibody specific to Androgen Receptor (AR). (avivasysbio.com)
  • After TMB substrate solution is added, only those wells that contain Androgen Receptor (AR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. (avivasysbio.com)
  • Watch how to prepare and dispense the detection antibody for SMC ® assays. (sigmaaldrich.com)
ELISAs2
Dual-luciferase reporter3
  • The binding sites of miR-128-3p and TGFBR2 were predicted by Targetscan online software and confirmed by dual-luciferase reporter assay and RNA immunoprecipitation (RIP) assay. (degruyter.com)
  • Then we used miR-720 mimic, miR-720 inhibitor and dual luciferase reporter gene assays to prove miR-720 regulate insulin secretion by targeting Rab35 in MIN6 cells. (researchsquare.com)
  • Dual-luciferase reporter assay and RNA Immunoprecipitation (RIP) assay were manipulated to analyze the relationships of circ_0037658, miR-665, and ADAMTS5. (iasp-pain.org)
Intra-assay Precision1
  • Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Androgen Receptor (AR) were tested 20 times on one plate, respectively. (avivasysbio.com)
Reagent1
  • Reagent kits, Diagnostic. (who.int)
Antibodies6
  • Enzyme-Linked Immunosorbent Assay (ELISA) uses antibodies and color change to detect and quantify proteins or other small molecules. (genscript.com)
  • This HCV Ab ELISA test is an enzyme-linked immunosorbent assay for in vitro qualitative identification of IgG antibodies to hepatitis C virus in human serum/plasma. (rapidtest.com)
  • E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. (gentaur.com)
  • The plasma of all the samples were subjected to kaolin clotting time and the serum of forty-three (43) of the samples were run on an enzyme-linked immunosorbent assay (ELISA) for the semi quantitative determination of IgG and IgM anticardiolipin antibodies. (ispub.com)
  • This is an enzyme-linked immunosorbent assay (ELISA) for the semiquatitative determination of IgG and IgM anticardiolipin antibodies in human serum or plasma. (ispub.com)
  • Laboratory testing with a rapid conducted at that time showed that 6.1% of the residents assay suggested that a dengue virus (DENV) was the caus- in nearby regions of Uganda had specifi c antibodies to ative agent. (folkhalsomyndigheten.se)
Reactivity1
  • KARPAS-299, a human cell line known to express PD-L1 shows no reactivity with FastScan ™ Total PD-L1 ELISA Kit (Mouse Preferred) #41590. (cellsignal.com)
Vitro2
  • Scientific Methods: In vitro growth inhibition assays were completed with AHCC alone and in combination with PLD in panel of human cancer cell lines and findings confirmed in vivo in an ovarian cancer xenograft mouse model. (researchgate.net)
  • Major Findings: The in vitro growth inhibition assays demonstrated additive activity when AHCC is co-administered with PLD. (researchgate.net)
Diagnostic3
  • Diagnostic Automation also provides other Kits. (rapidtest.com)
  • The first three proposals, on protein-based diagnosis, diagnostic materials and assays for less reactogenic vaccines, have been approved for one year. (who.int)
  • In discussion, concern was expressed about the lack of broad access to diagnostic materials and the unavailability of kits for diagnosis. (who.int)
Detection11
  • ELISA Detection Kits contain all necessary reagents and buffers for performing 96-well plate ELISA. (genscript.com)
  • GenScript cAMP ELISA Detection Kit is a competition enzyme-linked immunosorbent assay which can be used for quantitative detection of cAMP (Adenosine 3', 5'-cyclic monophosphate) in samples such as serum, plasma, saliva, cell culture supernatant, and urine. (genscript.com)
  • GenScript Human IL-8 ELISA Detection Kit is a solid phase sandwich ELISA for quantitative measurement of human IL-8 in serum, plasma and cell culture supernatant. (genscript.com)
  • GenScript His Tag Protein Detection Kit is developed for the detection of His-tagged protein expression and its relative quantification using His-tagged protein standards of known molecular weight (M.W.). This kit is based on competitive ELISA, suitable for most systems, such as bacterial, yeast, mammalian cell lysates and cell culture supernatant. (genscript.com)
  • GenScript GST tag ELISA Detection Kit is designed to quantitate glutathione-S-transferase (GST) or GST-fusion protein in samples such as cell lysate using a proprietary sandwich ELISA procedure in just 60 minutes. (genscript.com)
  • Enzyme-linked immunosorbent assays (ELISA) have been around as one of the primary methods of analyte detection for more than four decades. (ddw-online.com)
  • P erhaps most significantly are the big increases in assay sensitivity and the widening of the dynamic range accomplished by using alternatives to the traditional colorimetric detection, or by the use of new platform technologies. (ddw-online.com)
  • This assay has high sensitivity and excellent specificity for detection of Androgen Receptor (AR). (avivasysbio.com)
  • SMC ® assays are run on the state-of-the-art SMCxPRO ® detection instrument. (sigmaaldrich.com)
  • Discover with confidence using MILLIPLEX ® multiplex immunoassays for Luminex ® while ensuring robust assay performance and an always expanding portfolio to meet your biomarker detection needs. (sigmaaldrich.com)
  • Detection of PD-L1 using FastScan ™ Total PD-L1 ELISA Kit (Mouse Preferred) #41590 on a number of cell lines are shown in the upper figure. (cellsignal.com)
Reagents3
  • Other automated instrumentation is designed to support the creation/coating of ELISA assay plates or contains cartridge-based delivery of assay reagents to reduce set-up time. (ddw-online.com)
  • These are supported by a wide range of ELISA reagents, assay and kits providing relative profiling or absolute quantitation of proteins across a wide range of study areas. (ddw-online.com)
  • 1977. Mutagenicity of some munition waste water chemicals and chlorine test kit reagents. (cdc.gov)
Sandwich4
  • GenScript Lentivirus Titer p24 ELISA Kit allows researchers to quickly determine the physical titer of any HIV-1-based lentivirus from cell culture supernatant using sandwich ELISA method. (genscript.com)
  • GenScript AAV2 Titer Capsid ELISA Kit is a Sandwich ELISA Kit that is designed for quantitatively measuring the total capsid titer of adeno-associated virus serotype 2 (AAV2) in testing samples during viral vector production. (genscript.com)
  • GenScript BSA ELISA Kit is a Sandwich ELISA kit that is designed for quantitative measurement of bovine serum albumin (BSA) in testing samples. (genscript.com)
  • The ELISA kit is based on standard sandwich enzyme-linked immunosorbent assay technology. (abcam.com)
Species2
  • Depending on the epitopes used human ELISA kits can be cross reactive to many other species. (gentaur.com)
  • A commercial latex agglutination (LAT) assay was used to re-examine the samples with doubtful and uncertain ELISA results (151 samples from 42 species). (biomedcentral.com)
Antigens1
  • GenScript offers a variety of ELISA kits to detect and quantify proteins and small molecule antigens in samples such as serum, plasma, saliva, cell culture supernatant, and urine. (genscript.com)
Commercially available4
  • FA extracted from serum lipids were methylated and analyzed on GC-MS. Commercially available ELISA kits were used to determine the serum inflammatory markers. (karger.com)
  • A set of 13 serum samples (3 from SCLC and 10 from NSCLC patients) was screened by enzyme-linked immunosorbent assay (ELISA), using a commercially available kit. (astm.org)
  • ELISA), and serologically positive samples were confirmed using commercially available Real Time RT-PCR Kits. (who.int)
  • Title : False-positive results with a commercially-available West Nile virus IgM ELISA kit Corporate Authors(s) : Centers for Disease Control and Prevention (U.S.);United States. (cdc.gov)
Sensitivity2
  • The digital counting of molecules thus improves the assay sensitivity and extends the assay dynamic range beyond what can be achieved with traditional methods. (sigmaaldrich.com)
  • Several studies of RAT kits report results of 95% specificity but only 70-90% sensitivity. (medscape.com)
Precision1
  • Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Androgen Receptor (AR) were tested on 3 different plates, 8 replicates in each plate. (avivasysbio.com)
Substrate1
  • The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. (avivasysbio.com)
Immunoassays1
  • ELISA tests are enzyme linked immunoassays to detect human, mouse or other proteins in serum, plasma, urine or biological fluids. (gentaur.com)
Indirect1
  • Serum samples previously tested positive in HEp-2 indirect immunofluorescence assay (IFA) with nuclear dense fine speckled pattern (AC-2) were selected for anti-DFS70 autoantibody ELISA analyses. (cdc.gov)
RESULTS2
  • Results for the two soluble factors most significantly and independently associated with survival were validated in an independent set of samples ( n = 51) using a separate assay system. (iospress.com)
  • The results were validated by using the commercial PSA enzyme-linked immunosorbent assay kit and showed high consistency. (cdc.gov)
Dense1
  • Nested PCR assays targeting the dense granule protein 7 (GRA7) gene and B1 gene were also used to detect Toxoplasma in DNA samples extracted from 10 liver or blood specimens from 8 animals. (biomedcentral.com)
Latex agglutination1
Quantitative measurement1
Human1
  • It is considered to be a prebiotic that cannot be hydrolyzed by digestive enzymes in the human small intestine but can be fermented by probiotics[ 4 ]. (ijpsonline.com)
Researchers1
Samples1
  • These ELISA test kits are the ideal solution for an accurate measurement of multiple samples in a very broad quantitation range. (romerlabs.com)
Humans1
  • DNMT3A is a key enzyme for de novo epigenetic methylation in humans. (frontiersin.org)
Reaction1
Method3
  • The salivary hormone assays are advocated for their noninvasive, easy sample collection method. (rapidtest.com)
  • Most Blood Bank ELISA kits follow a similar method. (rapidtest.com)
  • Method of measurement: Kiazist biochemical kit. (who.int)
Automation1
  • These include many vendors now addressing the automation of ELISA assays. (ddw-online.com)
Findings1
  • This review article uses as its starting point some of the findings of HTStec's ELISA Assay Trends 2012 survey and report, published in June 2012 (1). (ddw-online.com)
Questionnaire1
  • The demographic, questionnaire and phlebotomy examination files may be linked to the laboratory data file using the unique survey participant identifier SEQN. (cdc.gov)
Proteins1
  • CCN proteins function as core regulatory factors in the inflammatory microenvironment and are closely linked with HCC progression [ 6 ]. (biomedcentral.com)
Western1
  • Western blot assay was used to measure the protein levels of Bcl-2-related X protein (Bax), cleaved-caspase-3, MMP13, Aggrecan, and ADAMTS5. (iasp-pain.org)
Serum1
Levels4
  • Over the years many changes to the basic format have resulted in assay improvements, but some of the most recent look set to take the traditional ELISA to new levels. (ddw-online.com)
  • Enzyme-linked immunosorbent assays were used to investigate maternal plasma iNOS levels. (medscimonit.com)
  • Moreover, our recent transcriptomic analysis reveals a decline in mRNA levels of Cyp17a1 , encoding a steroidogenic enzyme, in 4- to 30-day cultures of 6 d pp mouse testes ( 10 ). (elifesciences.org)
  • VEGF levels were quantified using enzyme-linked immunosorbent assay. (molvis.org)
Content1
Time3
  • When ordering five or more kits , please contact us for processing time and pricing. (cellsignal.com)
  • Functional assays measure only free (physiologically active) protein S. Functional assays are usually based on either the prothrombin time (PT) or the activated partial thromboplastin time (aPTT). (medscape.com)
  • For PT-based functional protein S assays, the activated protein C cofactor activity of protein S is measured based on the degree of prolongation of a PT-based clotting time. (medscape.com)
Flow1
  • Cell proliferation ability and apoptosis were detected using Cell Counting Kit-8 (CCK-8), 5-ethynyl-2'-deoxyuridine (EDU), and flow cytometry assays. (iasp-pain.org)
Plasma2
  • When an assay on a range of mixture of normal and patient's plasma is done, the presence of Lupus anticoagulant deficiency is indicated by the different patterns of response obtained. (ispub.com)
  • With aPTT-based functional protein S assays, platelet-poor plasma is incubated at 37°C with the following: protein S-deficient plasma, phospholipid, a contact activator (eg, Kaolin), and either an excess of activated protein C or an activator of protein C .Typically, the incubation period is 1-4 minutes, after which calcium is added to initiate clotting. (medscape.com)
Test Kits2
  • For more information about ELISA Kits or Rapid Tests, IFA Kits, CLIA Test Kits, or Serology tests, please see our website home page, or contact our Customer Service Representative at 818-591-3030. (rapidtest.com)
  • AgraQuant ® M1 test kits are accurate and reliable enzyme-linked immunosorbent assays (ELISA) in a quantitative format. (romerlabs.com)
Infection1
  • and improving the understanding of the link between and Zika virus infection and other neurological illnesses. (cdc.gov)
Mouse1
  • The relationship between lysate protein concentration from untreated and IFN-γ treated NIH/3T3 cells and the absorbance at 450 nm as detected by FastScan ™ Total PD-L1 ELISA Kit (Mouse Preferred) #41590 is shown in the upper figure. (cellsignal.com)
Development2
  • More and more evidences ( 6 - 8 ) show that miRNAs are involved in the regulation of glucose metabolism, insulin synthesis and secretion, which are the core links in the development of T2D. (researchsquare.com)
  • Explore more about our Custom Assay Development & Innovation (CADI) services and request more info. (sigmaaldrich.com)
Primary1
  • The main (primary) application area for survey respondents' ELISA assays was immunology (17% selecting). (ddw-online.com)