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  • subunits
  • S. cerevisiae has six kinesins and a single dynein heavy chain (for review see Hildebrandt and Hoyt, 2000 ), but only the four nuclear kinesins-Cin8p, Kip1p, Kip3p, and Kar3p-are potential kinetochore subunits. (rupress.org)
  • mammalian
  • Simple models suggest that these differences arise because the mechanical structure of the mammalian kinetochore is more complex. (nih.gov)
  • ZW10
  • IC phosphorylation directs binding to zw10 rather than dynactin, and this interaction is needed for kinetochore dynein localization. (rupress.org)
  • centromeric chromatin
  • Based on our results we propose a new model for inner centromeric chromatin architecture in which chromatin is folded as a layered boustrophedon, with planar sinusoids containing interspersed CENP-A-rich and H3-rich subdomains oriented toward the outer kinetochore. (pnas.org)
  • Structural
  • Structural plasticity of the living kinetochore. (nih.gov)
  • Here, we have combined genetic manipulations with deconvolution and super-resolution fluorescence microscopy for a detailed structural analysis of chicken kinetochores. (pnas.org)
  • endogenous
  • Overexpression of a mutant that lacked the amino-terminal 803 amino acids of CENP-E was found to saturate limiting binding sites on kinetochores and competitively blocked endogenous CENP-E from assembling onto kinetochores. (rupress.org)
  • chromosomes attached
  • The kinetochores of isolated HeLa cell chromosomes attached to an electron microscope specimen grid, fixed in formaldehyde, and stained with alcoholic phosphotungstic acid are visible as dark, preferentially stained structures distinct from the chromatin with which they are associated. (pnas.org)