Kinesin proteins. Medical search. Frequent questions

KIF11 (also known as kinesin-5 and Eg5) is a homotetramer which cross-links anti-parallel microtubules in the mitotic spindle to maintain spindle bipolarity. (wikipedia.org)
Kinesin-5 motors assemble into a bipolar homotetrameric structure that is capable of sliding apart bundles of anti-parallel oriented microtubules. (wikipedia.org)
In most eukaryotic cells, Kinesin-5 is thought to form cross-bridges between pairs of oppositely oriented microtubules in prophase and prometaphase and drives apart duplicated centrosomes during the formation of the mitotic spindle. (wikipedia.org)
Also, the binding of the Kinesin-5 inhibitors to an allosteric site on the motor interrupts the mechanism by which this enzyme converts the chemical energy of ATP hydrolysis into the mechanical work of moving microtubules, thus providing insight on how this enzyme works. (wikipedia.org)
There are many models that attempt to explain the self-assembly of the mitotic spindle based upon microtubules as a structural element, and a set of microtubule motors, including Kinesin-5 to move and order them. (wikipedia.org)
Control reactions were carried out in the absence of motor protein and in the absence of microtubules, both control reactions gave negligible ATPase activity (data not shown). (cytoskeleton.com)
Knockdown of Kif2a, but not other microtubule depolymerization or severing proteins, protects axonal microtubules from disassembly upon trophic deprivation. (nih.gov)
Kinesin-8s are dual-activity motor proteins that can move processively on microtubules and depolymerize microtubule plus-ends, but their mechanism of combining these distinct activities remains unclear. (nature.com)
It walks along microtubules, with the job of transporting proteins that are essential to the health of the cell. (satnavmag.com)
Molecules can be transported around a cell by so-called motor proteins that move along a network of filaments called microtubules. (elifesciences.org)
The head of the non-motor protein cannot bind to ATP, although it can bind to microtubules. (elifesciences.org)
mRNAs are transported along microtubules (MTs) by different kinesins and cytoplasmic dynein and get anchored at the actin cortex, intermediate filaments or unknown structures (1, 3). (europa.eu)
Living cells contain tiny transport systems made from a pair of proteins -- microtubules and kinesin -- that act much like railroad tracks and railroad cars. (trnmag.com)
The researchers coated tiny synthetic tracks with the motor molecule kinesin and coaxed microtubules to glide across the kinesin surface. (trnmag.com)
Cytoskeleton-associated proteins (CAPs) are involved in the organisation of microtubules and transportation of vesicles and organelles along the cytoskeletal network. (embl.de)

The discovery of small chemical inhibitors of human Kinesin-5 through a pioneering in vitro phenotypic screening on cancer cell lines has led to both the development of new anticancer therapeutic agents, and to novel tools to probe the mechanism of microtubule motor proteins. (wikipedia.org)
This toolkit of allosteric inhibitors has been used to probe the specific role of Kinesin-5 in mitotic spindle assembly as well as fine dissection of motor domain function. (wikipedia.org)
In recent years, melanin cell biology has become a broader research field and several important proteins contributing to melanogenesis and melanosome transport have been elucidated, paying the way for identifying melanin synthesis inhibitors. (frontiersin.org)

Here, we identify the kinesin superfamily protein 2A (KIF2A) as a key executor of microtubule disassembly and axonal breakdown during axonal pruning. (nih.gov)
KIF3A/B: a heterodimeric kinesin superfamily protein that works as a microtubule plus end-directed motor for membrane organelle transport. (rupress.org)

The human mitotic kinesin Eg5 represents a novel mitotic spindle target for cancer chemotherapy. (strath.ac.uk)
Crystal Structure of the Mitotic Kinesin Eg5 in Complex with Mg-ADP. (expasy.org)

Kinesin-like protein KIF11 is a molecular motor protein that is essential in mitosis. (wikipedia.org)
Kinesin-like protein KIF11 is a member of the kinesin superfamily, which are nanomotors that move along microtubule tracks in the cell. (wikipedia.org)
The conserved motor domain of human kinesin Eg5 (KIF11) was expressed in a prokaryotic system. (cytoskeleton.com)

This gene is a member of the KIF27 (kinesin 4) sub-family of the mammalian kinesin family. (nih.gov)
The kinesin- homologous protein encoded by the Chlamydomonas FLA10 gene is associated with basal bodies and centrioles. (bio.net)
Disrupted protein interactions identify gene sets involved in risk for ASD. (springer.com)
Around 60-80% of cases1 are caused by mutations of the gene that encodes optic atrophy protein 1 (OPA1), a protein that has a key role in inner mitochondrial membrane fusion and remodelling of cristae and is crucial for the dynamic organization and regulation of mitochondria2. (bvsalud.org)

Members of this protein family are known to be involved in various kinds of spindle dynamics and essential for mitosis. (wikipedia.org)
The human kinesin-5 protein has been actively studied for its role in mitosis and its potential as a therapeutic target for cancer. (wikipedia.org)
Loss of Kinesin-5 function from the onset of mitosis in most eukaryotic organisms examined, including animals, plants, and fungi, results in catastrophic failure of mitosis. (wikipedia.org)
The one exception to this effect is mitosis within the nematode, C. elegans, in which Kinesin-5 is not strictly essential for mitosis, but nonetheless has considerable impact on the overall fidelity of cell division. (wikipedia.org)
Through this work it was found that, in mammalian cells, Kinesin-5 is required for the initial assembly of the mitotic spindle during prophase and prometaphase, but is dispensable to traverse subsequent anaphase during a round of mitosis. (wikipedia.org)
We found that the relative concentration of the kinesin-14 family protein Pkl1 is decreased in meiosis I compared to mitosis, while the concentration of the kinesin-5 family protein Cut7 remains constant. (divide-eunetwork.org)
Our findings suggest that kinesin-8 family members have developed biochemically distinct activities throughout evolution and have implications for how Kif18A affects kinetochore-microtubule plus-end dynamics during mitosis in animal cells. (nih.gov)
Familia de adenosina-trifosfatasas mecánicas asociadas a los microtúbulos, que emplean la energía de la hidrólisis del ATP para mover orgánulos a lo largo de los microtúbulos en procesos como la mitosis, la meiosis y el transporte axónico. (bvsalud.org)

PEPCF expresses proteins in bacteria, insect and mammalian cells and uses a variety of chromatographic and biophysical techniques for protein purification and characterization. (embl.org)
The facility expresses and purifies proteins from E. coli , insect cells and mammalian cells. (embl.org)

Is an ortholog of human KIFAP3 (kinesin associated protein 3). (wormbase.org)
Is an ortholog of human KIF3A (kinesin family member 3A). (wormbase.org)
Is an ortholog of human KIF17 (kinesin family member 17). (wormbase.org)

It is thought that motor or adaptor proteins recognise different features on the surface of mRNPs with different affinities. (europa.eu)
It is still not known which RBPs or adaptor proteins are essential to recruit microtubule-binding motor proteins to mRNPs and to which extend this varies between different transported mRNAs. (europa.eu)
The coat on the budding vesicle comprises two layers, an inner layer of adaptor proteins (gray ovals) and an outer layer that forms a polyhedral cage. (biologists.com)

The recombinant protein contains a GST-Tag at the amino terminal end and has a combined molecular weight of 74 kDa. (cytoskeleton.com)
Molecular basis of mRNA transport by a kinesin-1-atypical tropomyosin complex. (embl.org)
Neuronal Calcium Sensor Protein, otherwise referred to as NCS, is a substantial protein family that operates as calcium-dependent molecular switches. (prospecbio.com)
When the iv mouse mutation was cloned, it was found to encode a molecular motor protein, an axonemal dynein, and was named lrd , for left-right dynein (human homolog is DNAH11 / DNAHC11 ). (medscape.com)

Currently, there are over 70 different eukaryotic kinesin-5 proteins identified by sequence similarity. (wikipedia.org)
With the establishment of genome sequence data for a substantial number of eukaryotes, it is now possible for the first time to analyze the complete kinesin repertoires of a diversity of organisms from most eukaryotic kingdoms. (ox.ac.uk)
The paralogue distribution suggests that the eukaryotic cenancestor possessed nearly all kinesin families. (ox.ac.uk)

Neuronal Calcium Sensor Proteins can regulate signal transduction while showing exceptional conservation of yeast to humans. (prospecbio.com)

These data reveal that the microtubule depolymerization activity of kinesin-8 originates from conformational changes of its motor core that are amplified by dynamic contacts between its extended loop-2 and tubulin. (nature.com)
We propose that these tubulin shape-induced alternations between pro-microtubule-depolymerization and pro-motility kinesin states, regulated by loop-2, are the key to the dual activity of kinesin-8 motors. (nature.com)
A lack of high-resolution structures capturing a kinesin-8 motor domain at each major step of its motile and microtubule depolymerization cycles has limited our understanding of the relationship between these two catalytic cycles and the structural elements of the motor domain that control them. (nature.com)
At this time, our knowledge of the catalytic cycles of kinesins is limited to motile kinesins that are incapable of microtubule depolymerization, and to microtubule-depolymerizing kinesins that are non-motile. (nature.com)

We show that guanylate kinase-associated kinesin (GAKIN), a kinesin-like motor protein, directly interacts with a PIP 3 -interacting protein, PIP 3 BP, and mediates the transport of PIP 3 -containing vesicles. (silverchair.com)
Predicted to enable protein kinase regulator activity. (wormbase.org)
J147 played these roles mainly by activating the extracellular signal-regulated protein kinase (ERK) pathway. (frontiersin.org)
Activation of cAMP protein kinase A (PKA) stimulates pigmentation through cAMP response element binding protein (CREB) dependent upregulation of MITF expression ( Rodríguez and Setaluri, 2014 ). (frontiersin.org)
The canonical TGFβ signalling pathway involves ligand-dependent assembly of a heteromeric receptor complex, receptor-kinase activation and subsequent phosphorylation and activation of SMAD proteins, which are transcriptional regulators that consequently accumulate in the nucleus. (nature.com)

On curved microtubule ends, loop-1 inserts into preceding motor domains, forming head-to-tail arrays of kinesin-8s that complement loop-2 contacts with curved tubulin and assist depolymerization. (nature.com)
An important gap in our understanding of the kinesin-8 depolymerization mechanism is the lack of a kinesin-8 structure bound to curved tubulin. (nature.com)

We investigate the functional relevance of de novo missense variants, specifically whether they are likely to disrupt protein interactions, and nominate novel genes in risk for ASD through integrated genomic, transcriptomic, and proteomic analyses. (springer.com)
Utilizing our previous interactome perturbation predictor, we identify a set of missense variants that are likely disruptive to protein-protein interactions. (springer.com)
Finally, we integrate protein interactions and cell-type-specific co-expression networks together with published association data to implicate novel genes in ASD risk in a cell-type-specific manner. (springer.com)
Extending earlier work, we show that de novo missense variants that disrupt protein interactions are enriched in individuals with ASD, often affecting hub proteins and disrupting hub interactions. (springer.com)
Consistent with other studies, genes identified by disrupted protein interactions are expressed early in development and in excitatory and inhibitory neuronal lineages. (springer.com)
The combination of such self-organizational phenomena with canonical intermolecular interactions is most likely to control the release of membrane proteins from the ER into the secretory pathway. (biologists.com)
This process depends on the general physico-chemical features of the cargo membrane protein and on the interactions of these features with the collective properties of the bilayer, instead of the one-to-one intermolecular interactions that exist between discrete signals and their receptors. (biologists.com)
There are three distinct interactions you will find with NCS1 that include Kif21a, or kinesin family member 21A. (prospecbio.com)

Named from studies in the early days of discovery, it is also known as Kinesin-5, or as BimC, Eg5 or N-2, based on the founding members of this kinesin family. (wikipedia.org)

Protein purity is determined by scanning densitometry of Coomassie Blue stained protein on a 12% polyacrylamide gel. (cytoskeleton.com)
Purity of kinesin motor domain proteins. (cytoskeleton.com)

For membrane proteins, a third mechanism, based on the interaction of their transmembrane domain (TMD) with lipid microdomains, must also be considered. (biologists.com)
In this Commentary, I review evidence in favor of the idea that partitioning of TMDs into bilayer domains that are endowed with distinct physico-chemical properties plays a pivotal role in the transport of membrane proteins within the early secretory pathway. (biologists.com)

The motility and depolymerization cycles in purely motile and purely depolymerizing kinesins are thus very different. (nature.com)
Motility is a fundamentally important property of most members of the kinesin superfamily, but a rare subset of kinesins are also able to alter microtubule dynamics. (nih.gov)
Kinesin proteins: A phylum of motors for microtubule-based motility. (bio.net)

How can a little motor protein observed in neuroscience be responsible for our happiness, and how can it help us find meaning in our short lives? (satnavmag.com)
Therefore, the function of the kinesin proteins in neuroscience mirror Sisyphus' punishment. (satnavmag.com)

Mutations affecting zipcodes, RBPs or motor-proteins required for neuronal mRNA localization were shown to lead to severe neurodegenerative diseases as ALS, FXTAS and FXS (7), underlining the need to understand the mechanisms that drive neuronal mRNA transport. (europa.eu)

Such a "holistic" approach using 486 kinesin-like sequences from 19 eukaryotes and analyzed by Bayesian techniques, identifies three new kinesin families, two new phylum-specific groups, and unites two previously identified families. (ox.ac.uk)
Finally, we present a set of hidden Markov models that can reliably place most new kinesin sequences into families, even when from an organism at a great evolutionary distance from those in the analysis. (ox.ac.uk)
RNA binding proteins recognize these sequences and assemble with their mRNA target into mRNPs. (europa.eu)

Still, it is not clear whether all the structural elements required for spindle assembly are known, or how the motors, including Kinesin-5, might be regulated in space and time. (wikipedia.org)
We identified the second kinesin-14 family protein Klp2 and microtubule dynamics as factors necessary for proper meiotic spindle assembly. (divide-eunetwork.org)

In my laboratory researchers are examining the roles played by axonal cytoskeletal proteins in nervous system development, and in the etiology of Alzheimer's and other related neurodegenerative diseases. (uml.edu)

Part of axonemal heterotrimeric kinesin-II complex. (wormbase.org)

found that the Kar3-Cik1 motor binds to and transports a protein complex that is crucial for separating chromosomes during cell division. (elifesciences.org)
Part of kinesin complex. (wormbase.org)
Secretory proteins exit the endoplasmic reticulum (ER) in coat protein complex II (COPII)-coated vesicles and then progress through the Golgi complex before delivery to their final destination. (biologists.com)

In this study, we show that the yeast kinesin-14 Kar3 generates processive movement as a heterodimer with the non-motor proteins Cik1 or Vik1. (elifesciences.org)
Functional dissection of protein complexes involved in yeast chromosome biology using a genetic interaction map. (thebiogrid.org)

Abcam: antibodies, proteins, kits. (abcam.com)

Although Kinesin-5 is required in all cells during cell division, it does not appear to play a major role in the metabolism of most non-dividing cells. (wikipedia.org)
In most cells, kinesins tend to transport other molecules away from the center and towards the cell edge. (elifesciences.org)
They allow cells to configure protein networks locally and exclude proteins from locations where their activity is harmful. (europa.eu)
This is different from the way the proteins work in cells, where kinesin glides across microtubule tracks. (trnmag.com)
in cells, so-called motor proteins - such as kinesin and the muscle protein actin - carry out this task. (empa.ch)

For the recombinant protein purifications we implement a large array of chromatographic techniques. (embl.org)

Kinesin superfamily proteins are ubiquitous to all eukaryotes and essential for several key cellular processes. (ox.ac.uk)

Structural insights into human Kif7, a kinesin involved in Hedgehog signalling. (nih.gov)
Multiplex human papillomavirus serology based on in situ-purified glutathione S-transferase fusion proteins. (cdc.gov)

Usually they glide along other proteins, similar to a train on rails, and in the process "burn" ATP (adenosine triphosphate), the chemical fuel, so to speak, of the living world. (empa.ch)

The crystal structure of Caenorhabditis elegans F53F4.3 protein Q20728 CAP-Gly domain was recently solved ( PUBMED:12221106 ). (embl.de)
The crystal structure of the Caenorhabditis elegans F53F4.3 protein CAP-Gly domain has been solved. (embl.de)

The inhibition of these above proteins could significantly suppress melanosome transport, which is an important mechanism for developing skin whitening agents. (frontiersin.org)

We maintain a large database of expression vectors at the EMBL Protein Expression and Purification Core Facility. (embl.org)

Kinesins and protein kinases: key players in the regulation of microtubule dynamics and organization. (nih.gov)
The report scope of Kinesin Spindle Protein market covers the in-depth analysis, along with an overview of market dynamics such as driver, restraints that are expected to impact the market growth. (prbulletin.com)

The encoded protein contains an N-terminal motor domain which includes nucleotide-binding and microtubule-interacting regions, a stalk domain containing a predicted coiled coil motif and a C-terminal tail domain. (nih.gov)

Quality control of protein reagents for the improvement of research data reproducibility. (embl.org)

Inhibiting the localisation of this mRNA leads to a significant reduction of CamKIIa protein at postsynaptic densities and a strong reduction of cognitive abilities in animal models (12). (europa.eu)
Also in this specific case, it is not understood how RBPs, potential adaptors and motor proteins, which are essential for the transport of CaMKIIa-mRNA are mechanistically contributing to its correct localisation. (europa.eu)

Kinesins can have different structures, but most are made up of two subunits that are joined and work together to create a walking-like movement. (elifesciences.org)

At kinetochore-microtubule plus ends, the kinesin-8 family member Kif18A is essential to align mitotic chromosomes at the spindle equator during cell division, but how it accomplishes this function is unclear. (nih.gov)
The structure of the protein can vary depending on which family member you look at. (prospecbio.com)

Motor proteins such as Kinesin transport objects within the cell along microtubule tracks. (feedburner.com)
Liquid biopsy represents a promising alternative to invasive tissue biopsies in particular for cancer diagnosis as it enables the study of cell-free (cf) nucleic acids in the blood, which includes cfDNA and various cfRNAs (e.g., protein-coding, lncRNA, microRNA, etc. (frontiersin.org)
Using the newly released and larger ASC dataset, we confirm these observations and take them in several new directions: (1) By defining a set of genes encoding these disrupted protein interactors in ASD subjects and another for their siblings, we evaluate their expression patterns in developing brain from fetal to early postnatal development and within general cell types of brain tissue. (springer.com)
The modified cell-transport proteins could eventually be used to transport materials around labs-on-a-chip. (trnmag.com)
Defining the functional relationships between proteins is critical for understanding virtually all aspects of cell biology. (thebiogrid.org)

Anatomy1

Chemicals and Drugs2

Microtubules15

Inhibitors3

Superfamily protein2

Mitotic kinesin2

KIF113

Gene4

Mitosis8

Mammalian cells2

Ortholog3

Adaptor proteins3

Molecular4

Eukaryotic3

Humans1

Microtubule depolymerization4

Kinase5

Tubulin2

Interactions8

BimC1

Purity2

Membrane2

Motility3

Neuroscience2

Neuronal1

Sequences3

Spindle assembly2

Axonal1

Axonemal1

Complex3

Yeast2

Antibodies1

Cells5

Recombinant1

Eukaryotes1

Human2

Glide1

Caenorhabditis2

Mechanism1

Purification1

Dynamics2

Motor domain1

Reagents1

MRNA2

Structures1

Family member2

Cell5