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  • PMID
  • Kinesin [ PMID: 8542443 , PMID: 2142876 , PMID: 14732151 ] is a microtubule-associated force-producing protein that may play a role in organelle transport. (ebi.ac.uk)
  • The kinesin motor domain comprises five motifs, namely N1 (P-loop), N2 (Switch I), N3 (Switch II), N4 and L2 (KVD finger) [ PMID: 20587735 ]. (ebi.ac.uk)
  • They are also important for kinesin-mediated axonal transport of mitochondria to nerve terminals in Drosophila [ PMID: 12495622 ]. (ebi.ac.uk)
  • neuronal
  • The founding member of this superfamily, kinesin-1, was isolated as a heterotetrameric fast axonal organelle transport motor consisting of 2 identical motor subunits (KHC) and 2 "light chains" (KLC) via microtubule affinity purification from neuronal cell extracts. (wikipedia.org)
  • different kinesin
  • Electrostatic properties of different kinesin representatives are expressed as a color spectrum ranging from positive (blue) to negative (red). (ucsd.edu)
  • However, a different kinesin motor, KIF15, can rescue cell division when Eg5 is incapacitated, leading to chemotherapeutic resistance. (pnas.org)
  • Kif15
  • SB743921 is a kinesin spindle protein (KSP) inhibitor with K i of 0.1 nM, almost no affinity to MKLP1, Kin2, Kif1A, Kif15, KHC, Kif4 and CENP-E. Phase 1/2. (selleckchem.com)
  • Clinical trials of small-molecule inhibitors of Eg5 have been stymied by the development of resistance, attributable to mitotic rescue by a different endogenous kinesin, KIF15. (pnas.org)
  • intracellular
  • Kinesins were discovered as MT-based anterograde intracellular transport motors. (wikipedia.org)
  • Kinesins and cytoplasmic dyneins play essential roles in intracellular transport such as axonal transport and in the formation of the spindle apparatus and the separation of the chromosomes during mitosis and meiosis. (wikipedia.org)
  • They also make up the internal structure of cilia and flagella.They provide platforms for intracellular transport and are involved in a variety of cellular processes, including the movement of secretory vesicles, organelles, and intracellular macromolecular assemblies (see entries for dynein and kinesin). (wikipedia.org)
  • axonal
  • The active movement of kinesins supports several cellular functions including mitosis, meiosis and transport of cellular cargo, such as in axonal transport. (wikipedia.org)
  • characterization
  • In Kinesin Protocols, Isabelle Vernos and a panel of hands-on experts present their most productive and reproducible techniques for the identification, purification, and characterization of the kinesin superfamily of microtubule-dependent motors. (springer.com)
  • structural
  • The exact mechanism of Kinesin-13 activated microtubule depolymerization remains unclear, however, recent biochemical and structural studies revealed some more detailed class specific features enabling researchers to formulate a model. (wikipedia.org)
  • Still, it is not clear whether all the structural elements required for spindle assembly are known, or how the motors, including Kinesin-5, might be regulated in space and time. (wikipedia.org)
  • There are also advanced methods for the study of kinesins at the structural level. (springer.com)
  • Structural basis for the ATP-induced isomerization of kinesin. (nih.gov)
  • cargo
  • Most kinesins walk towards the positive end of a microtubule, which, in most cells, entails transporting cargo such as protein and membrane components from the centre of the cell towards the periphery. (wikipedia.org)
  • In most cases transported cargo binds to the kinesin light chains, at the TPR motif sequence of the KLC, but in some cases cargo binds to the C-terminal domains of the heavy chains. (wikipedia.org)
  • It has been previously known that kinesin move cargo towards the positive (+) end of a microtubule, also known as anterograde transport/orthograde transport. (wikipedia.org)
  • Kinesin cargo, however, is not just limited to the transfer of vesicles. (news-medical.net)
  • Using the Triton Resource , a medium-scale high performance computing system at UC San Diego's San Diego Supercomputer Center (SDSC), researchers developed computational simulations of kinesin 'engines' hauling cargo along microtubule rails within cells. (ucsd.edu)
  • The direction in which cargo is transported can be towards the plus-end or the minus-end, depending on the type of kinesin. (wikipedia.org)
  • KIF11
  • Monastrol is a cell-permeable small molecule inhibitor of kinesin-5(KIF11) which is essential for maintaining separation of the half-spindles. (selleckchem.com)
  • ATPase
  • Among the approaches discussed are methods to express and purify kinesins in different systems, to characterize microtubule-enhanced ATPase activity and motility properties, and to test microtubule destabilizing activity. (springer.com)