AnatomyOrganismsChemicals and DrugsAnalytical, Diagnostic and Therapeutic Techniques and EquipmentPhenomena and ProcessesInformation Science
Recombinant ProteinsPhosphatidylinositol 3-KinasesMAP Kinase Signaling SystemProtein-Serine-Threonine KinasesProtein KinasesProtein Kinase InhibitorsCalcium-Calmodulin-Dependent Protein Kinasessrc-Family KinasesRecombinant Fusion ProteinsMolecular Sequence DataProtein Kinase Cp38 Mitogen-Activated Protein KinasesCyclic AMP-Dependent Protein KinasesMitogen-Activated Protein Kinase 1Amino Acid Sequencep21-Activated KinasesMitogen-Activated Protein Kinase KinasesJNK Mitogen-Activated Protein KinasesBase SequenceMitogen-Activated Protein Kinase 3Cloning, MolecularProtein-Tyrosine KinasesCreatine KinaseDNA, RecombinantCyclin-Dependent KinasesCDC2 Protein KinaseMAP Kinase Kinase KinasesPhosphorylationCasein Kinase IIeIF-2 KinaseCell LineCasein KinasesKineticsRibosomal Protein S6 KinasesProtein BindingPyruvate KinaseThymidine KinaseTransfectionMAP Kinase Kinase 1Receptor Protein-Tyrosine KinasesMutationExtracellular Signal-Regulated MAP KinasesPhosphotransferases (Alcohol Group Acceptor)IsoenzymesMAP Kinase Kinase 4Substrate SpecificityBlotting, WesternSignal Transduction1-Phosphatidylinositol 4-KinaseMitogen-Activated Protein KinasesCDC2-CDC28 KinasesGenetic VectorsEscherichia coliSequence Homology, Amino AcidI-kappa B KinaseGlycogen Synthase Kinase 3rho-Associated KinasesAurora KinasesCells, CulturedBinding SitesProtein Kinase C-deltaProto-Oncogene ProteinsProtein Kinase C-alphaProtein Structure, TertiaryEnzyme ActivationDiacylglycerol KinaseAMP-Activated Protein KinasesRNA, MessengerProto-Oncogene Proteins c-aktGene ExpressionPlasmidsPhosphoproteinsFocal Adhesion Kinase 1Tumor Cells, CulturedTyrosineMyosin-Light-Chain KinaseVaccines, SyntheticDNA, ComplementaryApoptosisJanus Kinase 2Electrophoresis, Polyacrylamide GelIntracellular Signaling Peptides and ProteinsSerineDose-Response Relationship, DrugDNA PrimersFocal Adhesion Protein-Tyrosine KinasesImmunoblottingBaculoviridaeRibosomal Protein S6 Kinases, 90-kDaProtein Kinase C-epsilonRecombination, GeneticCricetinaeTime FactorsTetradecanoylphorbol AcetateMutagenesis, Site-DirectedCalcium-Calmodulin-Dependent Protein Kinase Type 2MAP Kinase Kinase Kinase 1Cell DivisionProtein Kinase C betaHeLa Cells
ProteinsProtein KinasesSerineAssayInhibitorsPathwaysPhosphorylationMitogen-activaTyrosine-protein kinaseArabidopsisColiNucleoside diphosEnzymeThymidine kinaseHumanVitroPhosphorylatesSpeciesMammalian cellsMetabolismCatalyticCellsCellularCascadesSequenceReceptorNascentActivityViralSubstrateVaccineEncodesAdipocytesConjugateInterleukinFamily
Proteins6
Protein Kinases8
  • We now provide evidence that two growth-regulated, nucleus- and cytoplasm-localized protein kinases, 90-kDa ribosomal S6 kinase (RSK) and mitogen-activated protein kinase (MAP kinase), contribute to the serum-induced phosphorylation of c-Fos. (nih.gov)
  • Homeodomain-interacting protein kinases (HIPKs) belong to the CMGC kinase family and are closely related to dual-specificity tyrosine phosphorylation-regulated kinases (DYRKs). (nature.com)
  • Homeodomain-interacting protein kinases (HIPKs) are an evolutionarily conserved kinase family comprising the members HIPK1, HIPK2, and HIPK3 in vertebrates, and, additionally, HIPK4 in mammals. (nature.com)
  • VEGF-induced pMF was attenuated by the MEK/ERK inhibitor U0126 [1,4-diamino-2,3-dicyano-1,4-bis( o -aminophenylmercapto)butadiene] and was abolished by the phosphotidinositol 3 kinase/Akt inhibitor LY294002 [2-(4-morpholinyl)-8-phenyl-1(4 H )-benzopyran-4-one hydrochloride], demonstrating that ERK mitogen-activated protein kinases and Akt are both required for full expression of VEGF-induced pMF. (jneurosci.org)
  • Mitogen-activated protein kinases (MAPKs or MPKs) are one of the most important and conserved signaling molecules in plants. (springer.com)
  • This gene encodes a member of the protein kinase C (PKC) family of serine/threonine protein kinases. (nih.gov)
  • Protein phosphorylation is a signalling mechanism that regulates most aspects of cellular life, and protein kinases, which carry out this post-translational modification, constitute the largest family of enzymes encoded by the human genome. (lu.se)
  • Purification and characterization of two protein kinases acting on the aquaporin SoPIP2;1. (lu.se)
Serine6
  • PIM-1 is a serine/ threonine kinase belonging to the group of calcium/calmodulin-regulated kinases (CAMK). (proteinkinase.biz)
  • Bachmann M, Möröy T. (2005) "The serine/threonine kinase Pim-1. (proteinkinase.biz)
  • MAP4K5 (KHS1) is a serine/threonine kinase that belongs to the GCK family of kinases and has been implicated as an upstream regulator of MAP kinase signaling pathways. (thermofisher.com)
  • HIPKs belong to the CMGC group of serine/threonine kinases and are part of the dual-specificity tyrosine phosphorylation-regulated kinase (DYRK) family. (nature.com)
  • C-terminally adjacent to the HID follows a proline, glutamate, serine, and threonine (PEST)-rich domain, mediating proteasomal degradation of these kinases. (nature.com)
  • This gene encodes a serine/threonine protein kinase that is involved in many different processes, including, cell signaling, microtubule organization and stability, and apoptosis. (antibodies-online.com)
Assay2
  • ZAT10 was phosphorylated by recombinant Arabidopsis MPK3 and MPK6 in a kinase assay. (springer.com)
  • Furthermore, ZAT10 was also phosphorylated by native MPK3 and MPK6 prepared from Arabidopsis plants in an in-gel kinase assay. (springer.com)
Inhibitors3
  • They differ biochemically from thymidine kinase from mammalian cells and are inhibited by specific inhibitors that do not influence the activity of mammalian thymidine kinases. (wikipedia.org)
  • For example, biologic therapies are associated with conjunctivitis , facial erythema, and arthralgia, and there are boxed warnings for Janus kinase inhibitors. (medscape.com)
  • Tyrosine kinase inhibitors block these functions. (medscape.com)
Pathways1
  • Here, we show that bergenin, a phytochemical isolated from tender leaves of Shorea robusta , activates the MAP kinase and ERK pathways and induces TNF-α, NO and IL-12 production in infected macrophages. (frontiersin.org)
Phosphorylation6
  • Mapk8 is a member of the MAP kinase group that is activated by dual phosphorylation at thr and tyr residues during exposure to stress such as UV irradiation. (creativebiomart.net)
  • Crystal structures of non-phosphorylated Pim-1 kinase demonstrate that the catalytic region adopts a constitutive active confirmation and phosphorylation does not influence its activity. (proteinkinase.biz)
  • The major phosphopeptides derived from biosynthetically labeled c-Fos correspond to phosphopeptides generated after phosphorylation of c-Fos in vitro with both RSK and MAP kinase. (nih.gov)
  • The phosphorylation sites identified for RSK (Ser-362) and MAP kinase (Ser-374) are in the transrepression domain. (nih.gov)
  • This study suggests a role for nuclear RSK and MAP kinase in modulating newly synthesized c-Fos phosphorylation and downstream signaling. (nih.gov)
  • Liu Y, Zhang S (2004) Phosphorylation of 1-aminocyclopropane-1-carboxylic acid synthase by MPK6, a stress-responsive mitogen-activated protein kinase, induces ethylene biosynthesis in Arabidopsis . (springer.com)
Mitogen-activa3
  • Orthologous to human MAPK1 (mitogen-activated protein kinase 1). (nih.gov)
  • Kovtun Y, Chiu WL, Tena G, Sheen J (2000) Functional analysis of oxidative stress-activated mitogen-activated protein kinase cascade in plants. (springer.com)
  • MAPK-Group (2002) Mitogen-activated protein kinase cascades in plants: a new nomenclature. (springer.com)
Tyrosine-protein kinase1
  • Receptor that binds specifically to PDGFB and PDGFD and has a tyrosine-protein kinase activity. (abcam.com)
Arabidopsis5
  • The gene encoding 5'-adenylylsulfate (APS) kinase (EC 2.7.1.25) (APK) was cloned from Arabidopsis thaliana. (researchwithrutgers.com)
  • Lee, S & Leustek, T 1998, ' APS kinase from Arabidopsis thaliana: Genomic organization, expression, and kinetic analysis of the recombinant enzyme ', Biochemical and Biophysical Research Communications , vol. 247, no. 1, pp. 171-175. (researchwithrutgers.com)
  • Bethke G, Unthan T, Uhrig JF, Poschl Y, Gust AA, Scheel D, Lee J (2009) Flg22 regulates the release of an ethylene response factor substrate from MAP kinase 6 in Arabidopsis thaliana via ethylene signaling. (springer.com)
  • Droillard M, Boudsocq M, Barbier-Brygoo H, Lauriere C (2002) Different protein kinase families are activated by osmotic stresses in Arabidopsis thaliana cell suspensions. (springer.com)
  • Ichimura K, Mizoguchi T, Yoshida R, Yuasa T, Shinozaki K (2000) Various abiotic stresses rapidly activate Arabidopsis MAP kinases ATMPK4 and ATMPK6. (springer.com)
Coli3
  • MAPK13 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 385 amino acids (1-365) and having a molecular mass of 44.2 kDa. (prospecbio.com)
  • Recombinant full-length mouse JNK1 was expressed in E. coli cells using an N-terminal GST tag. (creativebiomart.net)
  • Purified and dephosphorylated recombinant human PIM-1 expressed in E.coli. (proteinkinase.biz)
Nucleoside diphos2
  • Also displays broad nucleoside diphosphate kinase activity. (enquirebio.com)
  • Thymidine monophosphate, the product of the reaction catalyzed by thymidine kinase, is in turn phosphorylated to thymidine diphosphate by the enzyme thymidylate kinase and further to thymidine triphosphate by the enzyme nucleoside diphosphate kinase. (wikipedia.org)
Enzyme5
  • One unit of 110a/p85a kinase activity is defined as the amount of enzyme required to produce 1 pmol of Phosphoinositol-3, 4,5-Trisphosphate/min at 37°C. (sigmaaldrich.com)
  • Thymidine kinase is an enzyme, a phosphotransferase (a kinase): 2'-deoxythymidine kinase, ATP-thymidine 5'-phosphotransferase, EC 2.7.1.21 that catalyzes the reaction: Thd + ATP → TMP + ADP where Thd is (deoxy)thymidine, ATP is adenosine 5'-triphosphate, TMP is (deoxy)thymidine 5'-phosphate and ADP is adenosine 5'-diphosphate. (wikipedia.org)
  • Thymidine kinase 1 is such a salvage enzyme, whereas thymidine kinase 2 is not cell cycle-dependent. (wikipedia.org)
  • Thymidine kinase is a salvage enzyme that is only present in anticipation of cell division. (wikipedia.org)
  • The enzyme deoxycytidine kinase phosphorylates this compound into an active 5+-triphosphate derivative, which, in turn, breaks DNA strands and inhibits DNA synthesis. (medscape.com)
Thymidine kinase8
  • Given the lack of effective treatments, we constructed a bifidobacterial recombinant thymidine kinase (BF-rTK) -ganciclovir (GCV) targeting system (BKV) to meet this requirement and to explore antitumor mechanisms. (biomedcentral.com)
  • The Herpes Simplex Virus thymidine kinase/ganciclovir (HSV-TK + GCV) system is currently one of the best-studied tumor suicide gene therapy systems [ 9 - 11 ]. (biomedcentral.com)
  • A colorectal cancer model was used to decipher the molecular mechanism of BF-rTK + GCV (bifidobacterial recombination thymidine kinase/ganciclovir) using a human apoptosis antibody array kit in a murine cancer model in vivo . (biomedcentral.com)
  • Mammals have two isoenzymes that are chemically very different, Thymidine Kinase 1 (TK1) and Thymidine Kinase 2 (TK2). (wikipedia.org)
  • It is present during cell division in much higher concentrations than TK2 and it is released in quantities that completely dominate the thymidine kinase activity in blood and other body fluids. (wikipedia.org)
  • Determination of viral thymidine kinase has been suggested for confirmation of diagnosis and for control of treatment of viral infections. (wikipedia.org)
  • In normal subjects, the amount of thymidine kinase in serum or plasma is, therefore, very low. (wikipedia.org)
  • The thymidine kinase level in serum, therefore, serves as a measure of malignant proliferation and, indirectly, as a measure of the aggressivity of the tumor. (wikipedia.org)
Human13
  • Shah N, Pang B, Yeoh KG, Thorn S, Chen CS, Lilly MB, Salto-Tellez M. (2008) "Potential roles for the PIM1 kinase in human cancer - A molecular and therapeutic appraisal" Eur J Cancer. (proteinkinase.biz)
  • A DNA sequence encoding the Homo sapiens (Human) AK1 / Adenylate kinase 1, was expressed in the hosts and tags indicated. (enquirebio.com)
  • Recombinant Human PDGF-AA Protein (HEK293) is a potent mitogen for a variety of cell types, including smooth muscle cells, connective tissue cells, bone and cartilage cells, and some blood cells. (abmole.com)
  • Recombinant Human IL-11 Protein (HEK293) is a multifunctional cytokine produced by stromal cells such as fibroblasts, epithelial cells and osteoclasts. (abmole.com)
  • Recombinant Human VEGF165 Protein (HEK293, C-His) is a powerful growth and angiogenic cytokine, it promotes the proliferation and survival of endothelial cells, promotes angiogenesis and vascular permeability. (abmole.com)
  • Recombinant Human TGF-β1 (HEK293) regulates cell proliferation, growth, differentiation and cells movement. (abmole.com)
  • Recombinant Human PKLR is produced by our Mammalian expression system and the target gene encoding Met1-Ser574 is expressed with a 6His tag at the C-terminus. (bonopusbio.com)
  • The following product was used in this experiment: Human MAP4K5 (KHS1), GST Tag Recombinant Protein from Thermo Fisher Scientific, catalog # PV3682. (thermofisher.com)
  • Inhibition of DNA-dependent protein kinase catalytic subunit by small molecule inhibitor NU7026 sensitizes human leukemic K562 cells to benzene metabolite-induced apoptosis. (cdc.gov)
  • Recombinant human ErB2 / Her2 (1129-ER) blocks anti-ErbB2 mediated inhibition of SK-BR-3 human breast cancer proliferation. (novusbio.com)
  • A PhaseⅡ of Injection for Recombinant Human Tissue Plasminogen Kinase Derivative in Treatment of Acute Ischemic Stroke. (nih.gov)
  • Tiago dos Reis Matos, MD, PhD, MSc , Amsterdam University Medical Centers, Amsterdam, the Netherlands, who was not involved in the study, told Medscape Medical News that "recombinant human interleukin-2 is an original therapy. (medscape.com)
  • Early data suggest that RAI is equally effective when used with thyroid hormone withdrawal or with recombinant human thyroid-stimulating hormone (rh-TSH) stimulation. (medscape.com)
Vitro2
  • Recombinant GM-CSF Protein is a growth factor that can support the in vitro colony formation of granulocyte-macrophage progenitors. (abmole.com)
  • These sites were verified by site-directed mutagenesis and in vitro kinase assays. (springer.com)
Phosphorylates2
  • It phosphorylates and activates protein kinase B (PKB) protein. (sigmaaldrich.com)
  • This kinase can be recruited to vesicle tubular clusters (VTCs) by direct interaction with the small GTPase RAB2, where this kinase phosphorylates glyceraldehyde-3-phosphate dehydrogenase (GAPD/GAPDH) and plays a role in microtubule dynamics in the early secretory pathway. (nih.gov)
Species1
  • Treatment with NU7026 did not alter the production of reactive oxygen species and oxidative stress by hydroquinone but repressed the protein level of DNA-PKcs and blocked the induction of the kinase mRNA and protein expression by hydroquinone. (cdc.gov)
Mammalian cells1
  • This kinase was shown to activate Jun kinase in mammalian cells, which suggested a role in stress response. (thermofisher.com)
Metabolism1
  • Phosphoinositide 3-kinases (PI3Ks) are a family of lipid-modifying enzymes that phosphorylate the 3′-OH group of inositol phospholipids and play key roles in physiology ranging from cell growth and metabolism to organismal development. (nature.com)
Catalytic3
  • PIK3CA (phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit a) is one of the four subunits of the 110kDa catalytic subunit of PI3K protein, which contains a regulatory region of 85kDa. (sigmaaldrich.com)
  • PIK3CA (phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit a) is one of the four catalytic subunits of PI3K protein, which plays an essential role in extracellular growth signaling. (sigmaaldrich.com)
  • We have previously reported that exposure of workers to benzene and to benzene metabolite hydroquinone in cultured cells induced DNA-dependent protein kinase catalytic subunit (DNA-PKcs) to mediate the cellular response to DNA double strand break (DSB) caused by DNA-damaging metabolites. (cdc.gov)
Cells3
  • Transient activation of AMP-activated protein kinase at G1/S phase transition is required for control of S phase in NIH3T3 cells. (nih.gov)
  • Effects of c-Src tyrosine kinase on ethanol sensitivity of recombinant NMDA receptors expressed in HEK 293 cells. (musc.edu)
  • This kinase is found to be necessary for BCL-ABL-mediated resistance to drug-induced apoptosis and therefore protects leukemia cells against drug-induced apoptosis. (nih.gov)
Cellular3
  • MAP kinases take part in an extensive range of cellular courses like differentiation, development, transcription regulation and proliferation. (prospecbio.com)
  • In addition to cellular TKs, virus specific thymidine kinases have been identified in Herpes simplex virus, Varicella zoster virus and Epstein-Barr virus. (wikipedia.org)
  • Cellular Basis for the Enhanced Efficacy of the Fms-Like Tyrosine Kinase 3 Ligand (FL) Adjuvanted VCG-Based Chlamydia abortus Vaccine. (cdc.gov)
Cascades1
  • Yeast SPS1/STE20 functions near the beginning of the MAP kinase signal cascades that is essential for yeast pheromone response. (thermofisher.com)
Sequence1
  • Within the HIPK family, HIPK1 and HIPK2 are the most closely related members, sharing about 93% sequence identity in their kinase domains, while HIPK3 is slightly less conserved with 87% identity. (nature.com)
Receptor4
  • Product Description google Tyrosine Kinase Receptor A (TrkA)-Fc Chimera, Purified Recombinant Protein, suitable to Block. (biosensis.com)
  • Scientific Background TrkA is a member of the neurotrophic tyrosine kinase receptor family. (biosensis.com)
  • VEGFA-165 signals by activating its receptor tyrosine kinase VEGF receptor-2 (VEGFR-2). (jneurosci.org)
  • Because another growth/trophic factor that signals via a receptor tyrosine kinase (brain derived neurotrophic factor) elicits a long-lasting facilitation of respiratory motor activity in the phrenic nerve, we tested the hypothesis that VEGFA-165 elicits similar phrenic motor facilitation (pMF). (jneurosci.org)
Nascent1
  • For the class II DYRK ortholog from D. melanogaster it was demonstrated that the critical tyrosine is cis -auto-phosphorylated by the nascent kinase in a transitory intermediate state during maturation at the ribosome 3 . (nature.com)
Activity2
  • Recombinant APS kinase shows maximal activity at 10 μM APS with 5 mM ATP, but it is inhibited at APS concentrations above 10 μM. (researchwithrutgers.com)
  • The recombinant xylose isomerase showed the highest activity at 85 degrees C with a specific activity of 1.0 U mg-1. (lu.se)
Viral1
  • Phosphatidylinositol 3-kinase type 2α (PI3KC2α) is an essential member of the structurally unresolved class II PI3K family with crucial functions in lipid signaling, endocytosis, angiogenesis, viral replication, platelet formation and a role in mitosis. (nature.com)
Substrate1
Vaccine2
  • We previously reported a C. abortus (Cab) vaccine comprising recombinant Vibrio cholerae ghosts (rVCG) expressing the conserved and immunogenic N-terminal region of the Cab polymorphic membrane protein D (rVCG-Pmp18.1) protein that protected mice against intravaginal challenge. (cdc.gov)
  • In this study, we investigated the immunomodulatory effect of the hematopoietic progenitor activator cytokine, Fms-like tyrosine kinase 3-ligand (FL) when co-administered with the rVCG-Pmp18.1 vaccine as a strategy to enhance the protective efficacy and the potential mechanism of immunomodulation. (cdc.gov)
Encodes1
Adipocytes2
  • Preferential activation of microsomal diacylglycerol/protein kinase C signaling during glucose treatment (De Novo phospholipid synthesis) of rat adipocytes. (jci.org)
  • Glucose has been reported to increase the de novo synthesis of diacylglycerol (DAG) and translocate and activate protein kinase C (PKC) in rat adipocytes. (jci.org)
Conjugate1
  • More than 40 patients were randomly assigned to receive one of two doses of a highly selective recombinant interleukin (IL)-2 conjugate, rezpegaldesleukin or placebo for 12 weeks, after which responders were observed out to 48 weeks. (medscape.com)
Interleukin1
Family2
  • MAPK13 belongs to the MAP kinase family. (prospecbio.com)
  • Presently, our main project evolves around the AMP-activated protein kinase (AMPK) family, which consists of AMPK and another 12 related kinases, of which we are particularly interested in the salt-inducible kinases (SIK) 1-3. (lu.se)