Kinase protein overexpression cell cycle. Medical search. Frequent questions

S-phase kinase-associated protein 2 (SKP2) is an important cell cycle regulator, targeting the cyclin-dependent kinase (CDK) inhibitor p27 for degradation, and is frequently overexpressed in breast cancer. (umass.edu)
Neratinib is an investigational, orally administered, irreversible inhibitor of the ErbB1 (EGFR), ErbB2 (HER2) and ErbB4 (HER4) kinases. (her2support.org)
This leads to stabilization and nuclear localization of FOXO3A resulting in transcriptional activation of the cyclin-dependent kinase inhibitor P27KIP1. (ku.edu)
In our study, cyclin-dependent kinase inhibitor 1A (CDKN1A), which participates in cell cycle regulation, was predicted and then validated as one target gene of miR-208b. (engreen.com.cn)
In vivo screen identifies a SIK inhibitor that induces β cell proliferation through a transient UPR. (scilifelab.se)
HG-9-91-01 is an inhibitor of salt-inducible kinases (SIKs), and overexpression of Sik1 specifically in β cells blocks the effect of HG-9-91-01 on β cell proliferation. (scilifelab.se)

[ 2 ] Options for second-line therapy in patients with relapsed/refractory disease include chemotherapy-free regimens with biologic targeted agents such as covalent Bruton tyrosine kinase (BTK) inhibitors, lenalidomide,venetoclax, and chimeric antigen receptor (CAR) T-cell therapy. (medscape.com)
We hypothesized that BMP4 might exert beneficial effects through modulation of p16 and p18 expression in transduced MDSCs since the cyclin-dependent kinase inhibitors p16INK4A (p16) and p18INK4C (p18) cause early G1-phase cell cycle blockade. (pitt.edu)
At molecular level, down-regulation of HSP70-2 resulted in reduced expression of cyclins, cyclin dependent kinases, anti-apoptotic molecules and mesenchymal markers and enhanced expression of CDK inhibitors, caspases, pro-apoptotic molecules and epithelial markers. (biomedcentral.com)
Novel Plant-specific cyclin-dependent kinase inhibitors induced by biotic and abiotic stresses. (helsinki.fi)
Due to the selective expression of UCK2 in malignancy cells, a selective inhibition of this important enzyme necessitates the discovery of its potential inhibitors for malignancy chemotherapy. (nanoker-society.org)
Here, we employed the used of to investigate the effectiveness of natural UCK2 inhibitors to cause HT-29 cell death. (nanoker-society.org)
We find that downstream target genes of the tumor suppressor, retinoblastoma protein, including genes encoding cell cycle regulators such as CDKs, cyclins and CDK inhibitors, are significantly upregulated in malignant mouse tumors in a CDK5-dependent manner. (oncotarget.com)
p27 is a member of the Kip family of cyclin-dependent kinase inhibitors and its overexpression results in cell cycle arrest at G1 and/or apoptosis. (spandidos-publications.com)
Similarly, depletion in the STRIPAK component STRIP1 affects activation of GCKIII kinases and cell cycle disruption through elevated expression of cyclin dependent kinase inhibitors p21 and p27, enhanced levels of which lead to a protective effect from therapeutic treatments and increased proliferation. (lu.se)

In mouse embryonic stem cells (ESCs), Cdc7 is needed for proliferation. (wikipedia.org)
JAM3 knockdown additionally inhibited trophoblast proliferation and increased the number of trophoblasts in the sub-G1 and G2/M phases, indicating cell-cycle disturbance and apoptosis. (bioone.org)
This phosphorylation event is associated with increased cell proliferation and poor prognosis in patients with glioma. (umass.edu)
Knockdown of KDM1B inhibits cell proliferation and induces apoptosis of pancreatic cancer cells. (cancerindex.org)
Inhibited HER2 activities include downstream signal transduction events and cell cycle regulatory pathways, such as the cell division cycle, ultimately resulting in decreased cell proliferation. (her2support.org)
HER2 positive breast cancer is identified by the overexpression of human epidermal growth factor receptor-2 (HER2) protein in tumor tissue and results in an increased rate of cell proliferation. (her2support.org)
Previous researches completed by our team have also demonstrated that resveratrol inhibits the proliferation, invasion, and metastasis of colorectal cancer cells [ 5 ]. (hindawi.com)
In most cancers, hSulf-1 is inactivated, which endows cancer cells with increasesed cell proliferation and metastatic activities, inhibition of apoptosis, and decreased sensitivity to radio- and chemotherapy. (oncotarget.com)
In this study, we found that hSulf-1 overexpression in melanoma cells can inhibit cell proliferation and induce cell cycle arrest and apoptosis by decreasing the protein kinase B (AKT) phosphorylation and limiting CDK4 nuclear import. (oncotarget.com)
It is a negative regulator of cell proliferation. (oncotarget.com)
In normal human tissues, hSulf-1 is stably expressed, and it plays important roles in cell proliferation and differentiation [ 1 , 2 ]. (oncotarget.com)
The decreased expression of hSulf-1 is closely related to cancer cell proliferation, increased metastatic activity, inhibition of apoptosis, and reduced sensitivity to radio- and chemotherapy [ 3 - 5 ]. (oncotarget.com)
One important mechanism of carcinogenesis is uncontrolled cell proliferation caused by the excessive activation of cell proliferation signals. (oncotarget.com)
Here, we have investigated the role of ChoK in the development of breast cancer and found that ChoK is both necessary and sufficient for growth factor-induced proliferation in primary human mammary epithelial cells and an absolute requirement for the specific mitogenic response to heregulin in breast tumor-derived cells. (aacrjournals.org)
These results demonstrate that ChoK plays an essential role in both normal human mammary epithelial cell proliferation and breast tumor progression. (aacrjournals.org)
Cell proliferation involves the replication of all cellular contents with the required energy for this to happen. (hindawi.com)
To prevent aberrant cell proliferation, these pathways are tightly regulated. (hindawi.com)
This activation is necessary for both cell proliferation as well as glucose uptake and use. (hindawi.com)
Down-regulation of EIF5B significantly decreased proliferation and invasiveness of the HCC cells. (techscience.com)
Many physiological processes, including proper tissue development and homeostasis, require a balance between apoptosis and cell 123653-11-2 manufacture proliferation. (bioinf.org)
Apoptosis and cell proliferation are linked by cell-cycle regulators and apoptotic stimuli that affect both processes. (bioinf.org)
A failure in regulating proliferation together with suppression of apoptosis are the 123653-11-2 manufacture minimal requirements for a cell to become cancerous [4]. (bioinf.org)
Two proteins involved intimately in regulating cell proliferation are Akt and the tumor suppressor p53 (p53). (bioinf.org)
p53 has an important protective role against undesired cell proliferation. (bioinf.org)
We have generated a mouse model, in which MTC proliferation is induced upon conditional overexpression of the CDK5 activator, p25, in C-cells, and arrested by interrupting p25 overexpression. (oncotarget.com)
Reducing CDK5 activity in human MTC cells down-regulated these cell cycle regulators suggesting that CDK5 activity is critical for cell cycle progression and MTC proliferation. (oncotarget.com)
However, whether miR-208b has effect on proliferation of muscle cell was under ascertained. (engreen.com.cn)
5-Ethynyl-20-deoxyuridine and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assays showed that overexpression of miR-208b promoted the proliferation of cattle primary myoblasts. (engreen.com.cn)
Therefore, we conclude that miR-208b participates in the cell cycle and proliferation regulation of cattle primary skeletal muscle cell through the posttranscriptional downregulation of CDKN1A. (engreen.com.cn)
MDA-MB-231 cells infected with human p27 shRNA (MDA-MB-231 hp27shRNA) showed increased proliferation compared to control MDA-MB-231 cells and MDA-MB-231 cells infected with mouse p27shRNA (MDA-MB-231 mp27shRNA). (spandidos-publications.com)
It is known that β cell proliferation expands the β cell mass during development and under certain hyperglycemic conditions in the adult, a process that may be used for β cell regeneration in diabetes. (scilifelab.se)
Here, through a new high-throughput screen using a luminescence ubiquitination-based cell cycle indicator (LUCCI) in zebrafish, we identify HG-9-91-01 as a driver of proliferation and confirm this effect in mouse and human β cells. (scilifelab.se)
Additional mechanistic studies indicate that HG-9-91-01 induces multiple signalling effectors downstream of SIK inhibition, including CRTC1, CRTC2, ATF6, IRE1 and mTOR, which integrate to collectively drive β cell proliferation. (scilifelab.se)
PFTK1 acts as a cyclin-dependent kinase that regulates cell cycle progression and cell proliferation. (signalchem.com)
Recently, signaling by GCC and its downstream effector, cyclic GMP (cGMP) has emerged as a principal regulator of proliferation in human colon cancer cells. (jefferson.edu)
In close agreement, induction of GCC signaling in mucosal sheets ex vivo and intestinal cells in vitro inhibited proliferation by activating cGMP-dependent protein kinase and delaying the cell cycle at the G1/S transition. (jefferson.edu)
Separately, deletion of GCC increased tumor growth by releasing a restriction on the cell cycle normally constraining epithelial cell proliferation. (jefferson.edu)

A successful therapy must therefore eliminate these cells known to be highly resistant to apoptosis. (hindawi.com)
In this paper, we describe the metabolic changes as well as the mechanisms of resistance to apoptosis occurring in cancer cells and cancer stem cells, underlying the connection between these two processes. (hindawi.com)
Further, we studied the effect of HSP70-2 protein ablation on signaling cascades involved in apoptosis, cell cycle and Epithelial-Mesenchymal-Transition both in culture as well as in-vivo human breast xenograft mouse model. (biomedcentral.com)
Depletion of HSP70-2 in MDA-MB-231 and MCF7 cells resulted in a significant reduction in cellular growth, motility, onset of apoptosis, senescence, cell cycle arrest as well as reduction of tumor growth in the xenograft model. (biomedcentral.com)
In addition, we have examined the putative role of HSP70-2 in apoptosis, cell cycle arrest and epithelial-mesenchymal transition (EMT) in-vitro and in-vivo xenograft mouse model using gene silencing approach. (biomedcentral.com)
Conclusions These findings ascertained an conversation between p53, c-myc, p21, p27, Bcl-2, PI3K/Akt pathway, and CF-induced apoptosis in MSTO-211H and HCT-116 cells, suggesting that CF acts as an important regulator of cell growth in human malignancy cell lines. (bioinf.org)
Apoptosis (programmed cell death) occurs in a wide variety of physiological NIK settings, where its role is to remove harmful, damaged or unwanted cells. (bioinf.org)
Moreover, induced Akt activity (p-AKT) (due to overexpression) is sufficient to block apoptosis triggered by many death stimuli [5]. (bioinf.org)
Although the conventional activities of p53 such as cell cycle arrest, senescence, and apoptosis are well accepted as the major checkpoints in stress responses, accumulating evidence implicates the importance of other tumor suppression mechanisms. (nature.com)
Is p53-dependent ferroptosis sufficient for tumor suppression in the absence of cell cycle arrest, senescence, and apoptosis? (nature.com)
To date, various mechanisms have been suggested to explain the powerful tumor-suppressive effect of p53, including the induction of cell cycle arrest, senescence, and apoptosis. (nature.com)
Apoptosis induction and activation of the mitogen-activated protein kinase pathway in human U-937 leukaemia cells by the synthetic flavanone 6-methoxy-2-(naphtalen-1-yl)-chroman-4-one. (ulpgc.es)
This compound induced (i) G2-M cell cycle arrest, (ii) apoptosis which was blocked by overexpression of the anti-apoptotic protein Bcl-2, and (iii) phosphorylation of p38 MAPK, extracellular-signal regulated kinases and c-jun N-terminal kinases / stress-activated protein kinases (JNK/SAPK) following different kinetics. (ulpgc.es)
Crypt hyperplasia in GCC-/- mice was associated with compensatory increases in cell migration and apoptosis. (jefferson.edu)

It has been suggested that the protein is essential for initiation of DNA replication and that it plays a role in regulating cell cycle progression. (wikipedia.org)
Use of the triple-negative TMX2-28 breast cancer cell line to address the role of SKP2 in cell cycle progression confirmed that SKP2 contributes to a more rapid cell cycle progression and may regulates pSer10p27 levels. (umass.edu)
Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis. (genetex.com)
Accumulating evidence shows that cancer stem cells are key drivers of tumor formation, progression, and recurrence. (hindawi.com)
EIF5B (Eukaryotic Translation Initiation Factor 5B) is a conserved eukaryotic translation factor that mediates association of the 40S and 60S ribosomal subunits during eukaryotic translation initiation, and modulates the cell cycle progression by regulating the translation of upstream open reading frames (uORF)-containing mRNAs such as p27 and p21 [ 3 ]. (techscience.com)
These data suggest that EIF5B functions as an oncogene that promotes cancer cell growth, survival, and progression. (techscience.com)
The expression of p53 is directly linked to a blockage of cell cycle progression at G0/G1 phase and upregulates Bax, cytochrome studies have shown the ability of the bioactive compounds of flavokawain B and alpinetin to target UCK2 enzyme specifically, inducing cell cycle arrest and subsequently leading to cancer cell death, possibly through interfering the MDM2-p53 signalling pathway. (nanoker-society.org)
All somatic cells proliferate via a mitotic process determined by progression through the cell cycle. (bioinf.org)
During cancer progression, some tumor cells acquire new characteristics, such as over-expression of epithelial-mesenchymal transition (EMT) markers, and undergo profound morphogenetic changes. (biomedcentral.com)
The orthologous gene product in humans can associate with and phosphorylate polo-like kinase 1, and overexpression of a kinase-dead version of the protein interferes with normal cell cycle progression. (utsouthwestern.edu)
In addition to its role as a regulator of cell cycle progression, p27 can also participate in cell motility, especially when it is mislocalized in the cytosol. (spandidos-publications.com)
Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of human cancers. (platcovid.com)
Nim1-related kinases coordinate cell cycle progression with the organization of the peripheral cytoskeleton in yeast. (microbiologyresearch.org)

NF-E2- related factor-2(Nrf-2), as well as mitogen-activated protein kinase (MAPK), is regulated by SFN. (nih.gov)

The protein is a serine-threonine kinase that is activated by another protein called either Dbf4 in the yeast Saccharomyces cerevisiae or ASK in mammals. (wikipedia.org)
The Cdc7/Dbf4 kinase complex, along with another serine-threonine kinase, cyclin-dependent kinase (Cdk), phosphorylates the pre-RC which activates it at the G1/S transition. (wikipedia.org)
SKP2 overexpression is associated with increased serine 10 phosphorylation of p27 (pSer10p27) in triple-negative breast cancer. (umass.edu)
Serine/threonine protein kinase Aurora B gene plays a regulatory role from G2 to cytokinesis, encompassing key cell cycle events such as centrosome duplication, chromosome bi-orientation, and segregation. (irdrjournal.com)
The protein serine/threonine kinase Akt (also known as protein kinase B or PKB) plays an important role in averting cell death. (bioinf.org)
FUNCTION: This gene encodes a member of the Ste20 family of serine/threonine protein kinases, and is similar to several known polo-like kinase kinases. (utsouthwestern.edu)
Using mass spectrometry, we show that MOV10 is phosphorylated in cells at the C-terminus, specifically at serine 970 (S970). (cdc.gov)

Cell-cycle arrest was associated with the engagement of checkpoint kinase 2-cell division cycle 25C-cyclin-dependent kinase 1/cyclin B1 signaling. (bioone.org)
The molecular consequence of translocation is overexpression of the protein cyclin D1 (coded by the PRAD1 gene located close to the breakpoint). (medscape.com)
The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. (genetex.com)
This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. (genetex.com)
WB analysis of HeLa cell lysate using GTX33611 Cyclin D1 antibody [RM241]. (genetex.com)
IHC-P analysis of human mantle cell lymphoma tissue using GTX33611 Cyclin D1 antibody [RM241]. (genetex.com)
We have recently reported a role for cyclin-dependent kinase 5 (CDK5) in MTC pathogenesis. (oncotarget.com)
We found that overexpression of miR-208b increased the expression of cyclin D1, cyclin E1, and cyclin-dependent kinase 2 at the levels of messenger RNA and protein in cattle primary myoblasts in vivo and in vitro. (engreen.com.cn)
Recombinant human full-length PFTK1 (CDK14) and Cyclin Y (2-end) were co-expressed by baculovirus in Sf9 insect cells using N-terminal GST tags. (signalchem.com)

In the glioma cells, EIF5B promotes cell survival by enhancing the translation of several IRES-containing mRNAs including those encoding anti-apoptotic proteins such as XIAP and Bcl-xL [ 4 ]. (techscience.com)

We tested whether natural killer cells expanded by co-culture with K562 cells transfected with 41BBL and membrane-bound interleukin-15 could kill myeloma cells with a high-risk gene expression profile in vitro and in a unique model which recapitulates human myeloma. (haematologica.org)
The suppression of the thermosensitive phenotype of a mak11 mutant by RLP24 overexpression and a direct in vitro interaction between Rlp24 and Mak11 suggest that Mak11 acts as an Rlp24 cofactor during early steps of 60S ribosomal subunit assembly. (pasteur.fr)
Here, we have shown that Cysteine-rich 61-Connective Tissue Growth Factor-nephroblastoma-overexpressed 5 (CCN5) induces growth arrest of TNBC cells in-vitro and in xenograft tumors. (ku.edu)
CCN5 treatment leads to an expression of functional ER-α in the TNBC cells, both in-vitro and in xenograft models, and sensitizes these cells to tamoxifen, commonly used for endocrine therapies. (ku.edu)
METHODS: CD73 knockout (CD73-/-) mice, wild-type (WT) mice, and AML-12 cells were used to evaluate the effect of CD73 on hepatocyte pyroptosis in vivo and in vitro. (bvsalud.org)
RESULTS: The protein expression of CD73 and pyroptosis pathway-associated genes was increased significantly in hepatocyte injury model both in vivo and in vitro. (bvsalud.org)
In vitro, overexpression of CD73 by pEGFP-C1/CD73 can decrease NLRP3 inflammasome activation and pyroptosis in hepatocytes. (bvsalud.org)
These results taken together suggest that BMP4 regulates the cell cycle of aged MDSCs and consequently promotes their osteogenic potential in vitro and in vivo. (pitt.edu)
In vitro GAP activity towards RhoA, Rac1 and Cdc42 is not a prerequisite for YopE induced HeLa cell cytotoxicity. (microbiologyresearch.org)
Mutation of the catalytic cysteinyl residue in the active phosphatase domain reduced the in vitro activity of the mutant protein to less than 0.5% of wild type activity, while mutation of the corresponding cysteinyl residue of the inactive phosphatase-like domain had no effect on in vitro activity. (embl.de)

Intriguingly, strong evidence has showed the dark side of Nrf-2: stable upregulation of Nrf-2-mediated survival pathway would protect cancer cells from a subset of chemotherapeutic agents tested. (nih.gov)
Choline kinase (ChoK), the enzyme responsible for the generation of phosphorylcholine (PCho) from its precursor choline, is the first enzyme in the Kennedy pathway that renders phosphatidylcholine as its final product. (aacrjournals.org)
Our studies show that after being secreted into the extracellular matrix, CCN5 binds to the α6β1 integrins of the cells leading to inhibition of the PI3K-AKT signaling pathway. (ku.edu)
Further analysis revealed that the phosphatidylinositol 3-kinase (PI3K)/protein kinase B (AKT) signaling pathway is a possible mechanism of CD73 regulation. (bvsalud.org)
In addition to its role in glucose metabolism, this pathway also regulates the redirection of free amino acids to protein synthesis via the mTOR-signaling pathway. (hindawi.com)
In the HCC cells, activation of the NF-kappa B signaling pathway and IkB phosphorylation was significantly reduced by EIF5B silencing. (techscience.com)
Introduction Uridine-cytidine kinase 2 (UCK2) is an enzyme that catalyses the conversion of uridine and cytidine to their monophosphate form of uridine and cytidine in an alternative salvage pathway of pyrimidine biosynthesis [1]. (nanoker-society.org)

Also, we found that the CCN5-induced PI3K-AKT inactivation leads to stabilization and nuclear accumulation of P27KIP1 resulting in cell cycle arrest of TNBC cells. (ku.edu)
Initial observations indicate that CCN5 induces expression of P16INK4A and P19ARF, resulting in cell cycle arrest of the tumor cells. (ku.edu)
The authors have previously reported that the Salmonella typhimurium SigD protein, a phosphatidylinositol phosphatase involved in invasion of the host cell, inhibits yeast growth, presumably by depleting an essential pool of phosphatidylinositol 4,5- bis phosphate, and also that a catalytically inactive version, SigD R468A , was able to arrest growth by a different mechanism that involved disruption of the actin cytoskeleton. (microbiologyresearch.org)
Second, growth inhibition by SigD R468A was mediated by a cell cycle arrest in G2 dependent on the Swe1 morphogenetic checkpoint, but SigD-induced growth inhibition was cell cycle independent. (microbiologyresearch.org)
In a screen for suppressors of SigD R468A -induced growth arrest by overexpression of a yeast cDNA library, the Cdc42 GTPase was isolated. (microbiologyresearch.org)
pps1Delta mutant strains did not differ from PPS1 strains under any of the conditions tested, but overexpression of the PPS1 protein in S. cerevisiae led to synchronous growth arrest and to aberrant DNA synthesis. (embl.de)
A screen for suppressors of this growth arrest identified the RAS2 gene as a multicopy suppressor of the PPS1p overexpression arrest. (embl.de)
We explore signalling alterations resulting in cancer stem cells as well as cell cycle arrest and cell fate determination. (lu.se)

Treatment of HeLa cells with paclitaxel induces phosphorylation of Aurora A at Thr288 while treatment with hydroxyurea ablates phosphorylation. (cellsignal.com)
In fact, several studies have shown that T2D induces senescence in multiple types of cells, including fibroblasts and endothelial cells ( 11 , 12 ). (frontiersin.org)
Ectopic expression of PtaRHE1, encoding a poplar RING-H2 protein with E3 ligase activity, alters plant development and induces defence related response in tobacco. (helsinki.fi)
CF treatment on leukemia cell lines induces cell death due to apoptotic mechanisms and altering 123653-11-2 manufacture cell metabolism through HIF-1 and GLUT-1 regulation [3]. (bioinf.org)
Single-cell transcriptomic analyses of mouse β cells demonstrate that HG-9-91-01 induces a wave of activating transcription factor (ATF)6-dependent unfolded protein response (UPR) before cell cycle entry. (scilifelab.se)

We further confirmed that hSulf-1 overexpression can inhibit AKT phosphorylation and CDK4 nuclear localization and retard the growth of melanoma xenograft tumors in nude mice. (oncotarget.com)
In normal cells, glucose participates in cellular energy production through glycolysis as well as through its complete catabolism via the tricarboxylic acid (TCA) cycle and oxidative phosphorylation (OXPHOS). (hindawi.com)

Scope includes mutations and abnormal protein expression. (cancerindex.org)
Certain mec1 mutations or overexpression of Mec1p lead to shortened telomeres and loss of telomeric silencing. (uky.edu)
However, cancer cells overcome these controls, in particular by acquiring genetic mutations leading to the activation of oncogenes (pten, myc) or loss of tumor suppressors (p53) [ 1 ]. (hindawi.com)
It is predicted that MCPH gene mutations may lead to the disease phenotype due to a disturbed mitotic spindle orientation, premature chromosomal condensation, signalling response as a result of damaged DNA, microtubule dynamics, transcriptional control or a few other hidden centrosomal mechanisms that can regulate the number of neurons produced by neuronal precursor cells. (biomedcentral.com)
Further, we review the corresponding genes and the proteins encoded by these genes, their possible role in the developing brain and reported mutations of these genes. (biomedcentral.com)
MCPH is inherited in an autosomal recessive pattern in which both copies of the gene in each cell have mutations. (biomedcentral.com)

The study suggests the possibility of MDM2 protein was downregulated and its suppression subsequently activates the expression of p53 during inhibition of UCK2 enzyme. (nanoker-society.org)
Therapeutic inhibition of Aurora kinase showed great promise as a probable anticancer regime because of its important role during cell division. (irdrjournal.com)
Increased manifestation levels of p53, p21, and p27, downregulation of c-myc and Bcl-2, and inhibition of Akt activation were also found in CF-treated MSTO-211 and HCT-116 cells. (bioinf.org)
Moreover, cell death was attenuated by the inhibition of mitogen-activated extracellular kinases and JNK/SAPK and was independent on reactive oxygen species generation. (ulpgc.es)
Infection of MDA-MB-231 cells with retroviruses harboring p27 short hairpin RNA (shRNA) designed from human p27 cDNA resulted in efficient inhibition of p27 expression, while p27 shRNA designed from mouse p27 cDNA did not affect p27 expression in MDA-MB-231 cells. (spandidos-publications.com)

For example, a major regulator of metabolism is phosphoinositol 3 kinase (PI3K). (hindawi.com)
A diverse range of physiological stimuli induce Akt kinase activity, including many trophic factors which promote survival, at least in part, through Akt activation via the phosphatidylinositide 3-OH kinase 123653-11-2 manufacture (PI3K) signaling cascade. (bioinf.org)

Cell division cycle 7-related protein kinase is an enzyme that in humans is encoded by the CDC7 gene. (wikipedia.org)
The rapid protocol (RP) PathScan ® RP Phospho-Aurora A (Thr288) Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of Aurora A protein phosphorylated at Thr288 in a reduced assay time of 1.5 hours. (cellsignal.com)
Nt5e (CD73) is a cell surface ecto-5'-nucleotidase, which is a key enzyme that converts the proinflammatory signal ATP to the anti-inflammatory mediator adenosine (ADO). (bvsalud.org)
Furthermore, HIF-1 actively limits the mitochondrial consumption of pyruvate at two levels: (i) through the enzyme PDK (PDH-kinase), which in turn inhibits PDH activity preventing the conversion of pyruvate into acetyl CoA, and thereby limiting mitochondrial metabolism of pyruvate [ 6 ] and (ii) through the direct activation of LDH [ 7 , 8 ]. (hindawi.com)
Abstract Uridine-cytidine kinase 2 is an enzyme that is overexpressed in abnormal cell growth and its implication is considered a hallmark of malignancy. (nanoker-society.org)
Overexpression of this enzyme have been implicated in several cancers and it is therefore considered a hallmark of cancer. (nanoker-society.org)

Avoid multiple freeze-thaw cycles. (genetex.com)
For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles. (signalchem.com)

Cloning and subcellular localization of a human phosphatidylinositol 3-phosphate 5-kinase, PIKfyve/Fab1. (microbiologyresearch.org)

Genome-scale GOF screening approaches have largely remained limited to the use of cDNA library overexpression systems. (cdc.gov)

These mice are devoid of endogenous natural killer and T-cell activity and were used to determine whether adoptively transferred expanded natural killer cells could inhibit myeloma growth and myeloma-associated bone destruction. (haematologica.org)
We found that mammary epithelium-specific overexpression of CCN5 in transgenic mice leads to an increase in ER-α expression and that this impact of CCN5 is not restricted to the normal cells. (ku.edu)

We have previously shown that budding yeast Mak11, whose homologues in other eukaryotes were described as modulating a p21-activated protein kinase function, accumulates in Rlp24-associated pre-60S complexes when their maturation is impeded in Saccharomyces cerevisiae. (pasteur.fr)

p53 is regulated by human double minute 2 (MDM2), an E3 ubiquitin ligase that targets and binds to p53 promoting ubiquitination and degradation of the protein [6,7]. (nanoker-society.org)

hSulf-1 reduces the sulfation of heparan sulfate proteoglycans (HSPGs) in the extracellular matrix and inhibits binding between various cell growth factors and their receptors. (oncotarget.com)
The p53 protein is a transcription factor that normally inhibits cell growth and stimulates cell death in response to myriad stressors, including DNA damage (induced by either UV or chemical agents such as hydrogen peroxide), oxidative stress, and deregulated oncogene expression [6-10]. (bioinf.org)

Cyclins function as regulators of CDK kinases. (genetex.com)

Single-cell imaging of ATP-reduced yeast mutants revealed that ATP levels in these mutants underwent stochastic and transient depletion, which promoted the cytotoxic aggregation of endogenous proteins and pathogenic proteins, such as huntingtin and α-synuclein. (waseda.jp)
Programmable DNA binding proteins have emerged as an exciting platform for engineering synthetic transcription factors for modulating endogenous gene expression 5 - 11 . (cdc.gov)

Expanded natural killer cells killed both allogeneic and autologous primary myeloma cells avidly via a perforin-mediated mechanism in which the activating receptor NKG2D, natural cytotoxicity receptors, and DNAX-accessory molecule-1 played a central role. (haematologica.org)
ErbB2 belongs to the ErbB family of receptor tyrosine kinases, which has been implicated in a variety of human cancers , including cancers of the breast. (her2support.org)
It can reduce the sulfation of cell surface heparan sulfate proteoglycan (HSPG) and inhibit various growth factor receptor-mediated signaling pathways. (oncotarget.com)
Patients with triple negative breast cancer (TNBC), where the cancer cells do not express nuclear hormone receptors and human epidermal growth factor receptor 2 (HER2), have worse survival rate compared to the patients with luminal subtypes of cancer. (ku.edu)
Next, we have shown that CCN5 protein can induce expression of estrogen receptor-α (ER-α) in mammary epithelial cells. (ku.edu)

The functional inactivation of WD40 repeat protein Mak11 interfered with the 60S rRNA maturation, led to a cell cycle delay in G(1), and blocked green fluorescent protein-tagged Rpl25 in the nucleoli of yeast cells, indicating an early role of Mak11 in ribosome assembly. (pasteur.fr)
Overexpression of MDM2 leads to inactivation of p53 tumour protein, thereby diminishing its tumour suppressor function [8]. (nanoker-society.org)
Cas9 nuclease can be converted into an RNA-guided DNA binding protein (dCas9) via inactivation of its two catalytic domains 12 , 13 and then fused to transcription activation domains. (cdc.gov)

It was revealed as early as in the 1930s that the behavior of the whole chromosome and its fragments in cells varies. (actanaturae.ru)

We also demonstrated that the −657 to +411 DCT promoter fragment efficiently directs RB cell-specific transcription of the luciferase reporter gene in cell lines. (molvis.org)
In contrast, cancer cells shift their metabolism toward lactate production even in the presence of oxygen [ 4 ], partly through genetic modifications that stabilize the transcription factor Hypoxia Inducible Factor (HIF) involved in the adaptation of the cells to hypoxia, under nonhypoxic conditions as well as generating an adaptive response to the hypoxic microenvironment (Figure 1 ). (hindawi.com)
During development, the blood vascular system arises from endothelial cell progenitors that differentiate from mesodermal cells, mostly through the expression of the transcription factor (TF) ETV2. (nature.com)
Transcription factors (TFs) are critical for B-cell differentiation, affecting gene expression both by repres- sion and transcriptional activation. (lu.se)
Among the established custom DNA binding domains, Cas9 is most easily scaled to facilitate genome-scale perturbations 3 , 4 due to its simplicity of programming relative to zinc finger proteins and transcription activator-like effectors (TALEs). (cdc.gov)

In the pathogenesis and treatment of liver disease, studies have found that the expression levels of key genes that promote or inhibit the development of liver disease are regulated by m6A modification, in which abnormal expression of reader proteins determines the fate of these gene transcripts. (bvsalud.org)
Here, we identify genes and proteins that are differentially expressed in proliferating versus arrested benign mouse MTC. (oncotarget.com)
Deep RNA-DNA, RNA-protein interaction studies, and phenotype rescue analyses reveal that LETR1 is a nuclear trans-acting lncRNA modulating, via key epigenetic factors, the expression of essential target genes, including KLF4 and SEMA3C , governing the growth and migratory ability of LECs. (nature.com)
Expected and potentially novel resistance genes are enriched in the top hits and are validated using individual sgRNA as well as cDNA overexpression. (cdc.gov)

Deletion of SCS2 resulted in decreased telomeric silencing, and the scs2 mutation increased the rate of cellular senescence observed for mec1-21 tell double mutant cells. (uky.edu)
Because age-related cellular senescence and type 2 diabetes (T2D) have been recognised as risk factors for CRC development, the recent finding that type 2 diabetic patients present an elevated circulating volume of senescent cells raises the question whether type 2 diabetes facilitates the process of CRC tumorigenesis by inducing premature cell senescence. (frontiersin.org)
Telomere shortening leads to the attainment of the Hayflick limit, the transition of cells to a state of senescence. (actanaturae.ru)

Furthermore, EIF5B knockdown suppressed epithelial-mesenchymal transition (EMT) and the cancer stem cell (CSC) phenotype. (techscience.com)
PHENOTYPE: Targeted disruption of this gene results in enhanced cell adhesion in mitogen-stimulated T cells. (utsouthwestern.edu)

Mec1p is a cell cycle checkpoint protein related to the ATM protein kinase family. (uky.edu)

The Cdc7 kinase is involved in regulation of the cell cycle at the point of chromosomal DNA replication. (wikipedia.org)
The gene, CDC7, is involved in the regulation of cell cycle because of the gene product Cdc7 kinase. (wikipedia.org)
Because the two proteins, Cdc7 and Dbf4, must form a complex before activating the MCM complex, the regulation of one protein is sufficient for both. (wikipedia.org)
The involvement of m6A in the regulation of gene expression depends on the specific recognition of m6A-modified RNA by reader proteins. (bvsalud.org)
Down-regulation of EIF5B also increased the sensitivity of HCC cells to 5-fluorouracil (5-FU). (techscience.com)
The Arabidopsis thaliana homolog of yeast BRE1 has a function in cell cycle regulation during early leaf and root growth. (helsinki.fi)
Loss of mitotic regulation is a possible cause of aneuploidy in human epithelial malignancy and it is thought to create an abnormal nuclear morphology in cancer cells. (irdrjournal.com)
These results indicated that miR-208b participates in the cell cycle regulation of cattle primary myoblast cells. (engreen.com.cn)
The tumour microenvironment (TME) contains several other cell types apart from cancer cells which play a role not only in the regulation of the environment but in response to treatments. (lu.se)

What pathways are this gene/protein implicaed in? (cancerindex.org)
The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
Lipids, amino acids, and nucleotides necessary for the biosynthesis of the daughter cells are mostly provided by intermediate metabolites of these pathways. (hindawi.com)
Once activated, p53 coordinates multiple downstream pathways, thereby maintaining the homeostasis of the host cell or organism (if the stress is mild, transient, and repairable) or eliminating damaged cells (if the stress is acute, prolonged, and difficult to resist). (nature.com)
Signaling pathways that are activated by intrinsic or extrinsic stimulation converge on the transcriptional factors and regulate phenotypic changes of cancer cells [ 9 ]. (biomedcentral.com)

In this study, we explore mechanisms in breast cancer cells that potentially lead to recurrence. (lu.se)
The main aim of the course is to enable students to acquire specialised knowledge and understanding of membrane biochemistry and the molecular structure, topology and functional mechanisms of membrane proteins. (lu.se)
A number of proteins from each process, for which the structure is known, are explored in greater detail in order to highlight the functional molecular mechanisms. (lu.se)
For chemotherapy drugs, one of the best characterized resistance mechanisms is overexpression of MDR1 , a cell membrane transporter that causes efflux of certain drugs (eg, vinca alkaloids, taxanes, anthracyclines). (msdmanuals.com)

Tyrosine specific protein phosphatases (PTPases) contain two conserved cysteines, the second one has been shown to be absolutely required for activity. (embl.de)
The deduced amino acid sequence of PPS1p shows similarity with protein-tyrosine phosphatases (PTPases) and is most closely related to a subfamily of PTPases that are capable of dephosphorylating phosphoseryl and phosphothreonyl residues as well as phosphotyrosyl residues. (embl.de)

Identification of mosquito proteins that differentially interact with alphavirus nonstructural protein 3, a determinant of vector specificity. (cdc.gov)
Mosquito proteins that differentially interacted with CHIKV nsP3 or ONNV nsP3 were identified. (cdc.gov)

Gene ID: 5925, OMIM 614041 ) gene, which encodes a tumor suppressor protein. (molvis.org)
This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. (genetex.com)

this means that most eukaryotic cells have the Cdc7 kinase protein. (wikipedia.org)
The genetic information in eukaryotic cells is stored in linear DNA molecules known as chromosomes [ 1 ]. (actanaturae.ru)

The Cdc7/Dbf4 complex adds a phosphate group to the minichromosome maintenance (MCM) protein complex allowing for the initiation of DNA replication in mitosis (as explained in the Cdc7 and Replication section below). (wikipedia.org)
After chromatin undergoes changes in telophase of mitosis, the hexameric protein complex of MCM proteins 2-7 forms part of the pre-replication complex (pre-RC) by binding to the chromatin and other aiding proteins (Cdc6 and Cdt1). (wikipedia.org)

Mantle cell lymphoma (MCL) is a lymphoproliferative disorder derived from a subset of naive pregerminal center cells localized in primary follicles or in the mantle region of secondary follicles. (medscape.com)
Mantle cell lymphoma (MCL) is recognized in the Revised European-American Lymphoma and World Health Organization classifications as a distinct clinicopathologic entity. (medscape.com)
This enabled identification of TFs that can be uniquely associated to the tumor cells of chronic lymphocytic leukemia (CLL), diffuse large B-cell lymphoma (DLBCL), follicular lymphoma (FL), hairy cell leukemia (HCL), and mantle cell lymphoma (MCL). (lu.se)

Membrane proteins play a key role in the cell's energy metabolism and in its signalling and communication with its environment. (lu.se)
More than half of all drugs that are in use today target membrane proteins. (lu.se)
The lectures address the three different main types of membrane proteins and associated cellular processes: transport and transporters, signal transduction and receptors, bioenergetics and photosynthetic and respiratory proteins. (lu.se)
Lectures dealing with methods for theoretical modelling of membrane protein structure, fusion protein techniques, X-ray crystallography, heterologous expression, solubilisation and purification of membrane proteins are also included in the course. (lu.se)
This is followed by experimental determination using genetic construction and expression of a fusion protein of the membrane protein and a marker protein in a bacterial system which is subsequently analysed. (lu.se)
In silico exercise addressing potential problems concerning the detection of heterologously expressed membrane proteins, solubilisation and evaluation of detergent properties, ion exchange chromatography and gel filtering in the presence of a detergent, and control of the protein's stability and integrity after purification. (lu.se)
Group discussions about e.g. the similarities/dissimilarities, cloning and overexpression strategies, and structure and function of membrane proteins. (lu.se)
An individually planned and executed minor project during two weeks, in which the students express a membrane protein of their choice and demonstrate in some way that the expression was successful. (lu.se)
The project entails practice in literature searching, project planning and documentation, and provides specialised practical knowledge of expression and management of membrane proteins. (lu.se)

Junctional adhesion molecule 3 (JAM3) is involved in epithelial cell junction, cell polarity, and motility. (bioone.org)
Overexpression of JAM3 or recombinant JAM3 protein enhanced trophoblast adhesion and migration, which were inhibited by JAM3 knockdown. (bioone.org)
Binding of this protein to CXCR3 results in pleiotropic effects, including stimulation of monocytes, natural killer and T-cell migration, and modulation of adhesion molecule expression. (cancerindex.org)
EMT is a process in which epithelial cells lose their cell polarity and cell-cell adhesion, and gain migratory and invasive properties, becoming mesenchymal cells [ 3 , 4 ]. (biomedcentral.com)

This suggested that overexpression of Nrf-2 resulted in enhanced resistance of cancer cells to chemotherapeutic agents. (nih.gov)
Lack of ER-α expression in TNBC cells or loss of ER-α activation after endocrine treatment of luminal cancers makes these breast cancer cells resistant to tamoxifen and other endocrine therapies. (ku.edu)
In contrast to normal cells, most cancer cells predominantly produce energy by a high rate of glycolysis followed by lactate fermentation, even in the presence of oxygen, a less efficient metabolism compared to a low rate of glycolysis followed by mitochondrial oxidation of pyruvate [ 2 ]. (hindawi.com)
Metabolic adaptations of cancer cells. (hindawi.com)
Glucose and glutamine are the 2 major substrates used by cancer cells. (hindawi.com)
HSP70-2 expression was also observed in all breast cancer cells (BT-474, MCF7, MDA-MB-231 and SK-BR-3) used in this study. (biomedcentral.com)
We also report that HSP70-2 plays an important role in cellular growth, migration and invasion of breast cancer cells and tumor growth of breast cancer xenograft. (biomedcentral.com)
Epithelial-mesenchymal transition (EMT) is a dynamic process involved in cancer metastasis wherein cancer cells with epithelial characteristics acquire mesenchymal characteristics such as increased motility, invasion, and survival [ 6 ]. (techscience.com)
Furthermore, cancer cells undergoing EMT phenotypic changes also acquire drug resistance [ 7 ]. (techscience.com)
A subset of cancer cells undergoing EMT acquire characteristics of stem cells and are designated as cancer stem cells (CSCs) [ 8 ]. (techscience.com)
To further elucidate the role of p27 in the motility of MDA-MB-231 breast cancer cells, we performed p27 knockdown in MDA-MB-231 cells by RNA interference. (spandidos-publications.com)
Lee EY, Jeon MJ, Yang W and Shin I: Effect of p27 on motility of MDA-MB-231 breast cancer cells. (spandidos-publications.com)
The surviving cells become cancer cells, which are capable both of dividing indefinitely and maintaining telomere length (usually with the aid of telomerase). (actanaturae.ru)
The ideal drug would target only cancer cells and have no adverse effects on normal cells. (msdmanuals.com)
Cytotoxic drugs damage DNA and kill many normal cells as well as cancer cells. (msdmanuals.com)
In contrast, other drugs (eg, DNA cross-linkers, also known as alkylating agents) have a linear dose-response relationship, killing more cancer cells at higher doses. (msdmanuals.com)

Adenosine triphosphate (ATP) at millimolar levels has recently been implicated in the solubilization of cellular proteins. (waseda.jp)
We herein demonstrated that AMP-activated protein kinase (AMPK) and adenylate kinase (ADK) cooperated to maintain cellular ATP levels regardless of glucose levels. (waseda.jp)
The present study demonstrates that cellular ATP homeostasis ensures proteostasis and revealed that suppressing the high volatility of cellular ATP levels prevented cytotoxic protein aggregation, implying that AMPK and ADK are important factors that prevent proteinopathies, such as neurodegenerative diseases. (waseda.jp)
To better understand the cellular components controlling alphavirus vector specificity, a cell culture model system of the anopheline restriction of CHIKV was developed along with a protein expression strategy. (cdc.gov)
Temporal distribution studies identify hnRNPA1 and NP co-localize in the same cellular milieu in both nucleus and mitochondria in NP-transfected and IAV-infected mammalian cells. (cdc.gov)
Although older chemotherapeutic drugs are often toxic to normal cells, advances in genetics and cellular and molecular biology have led to development of more selective drugs. (msdmanuals.com)

Recently obtained evidence reveals that epithelial-mesenchymal transition (EMT) of tumor cells causes metastasis, which is detrimental to patients' survival. (biomedcentral.com)

Overall, hSulf-1 function in melanoma cells provides an ideal molecular treatment target. (oncotarget.com)
Heat shock family of proteins (HSPs) is proposed to play pivotal role in breast tumor development owing to their intrinsic molecular chaperone properties [ 5 ]. (biomedcentral.com)
120 credits) in Chemistry and Molecular Biology and compulsory for a degree of Master of Science (120 credits) in Protein Science. (lu.se)

Although expression levels of the protein appear to be constant throughout the cell cycle, the protein kinase activity appears to increase during S phase. (wikipedia.org)
JAM3 expression in cell-cell junctions decreased with the formation of syncytiotrophoblasts. (bioone.org)
Furthermore, H 2 O 2 -induced oxidative stress reduced JAM3 expression in trophoblasts and cell culture supernatants. (bioone.org)
Expression of DCT was absent or barely detected in retina tissues, various human ocular cells, and major organs. (molvis.org)
The regulatory elements required for this cell-specific gene expression are likely located within its proximal promoter. (molvis.org)
In most cancer cell lines and tumor tissues, hSulf-1 expression levels are markedly decreased. (oncotarget.com)
Finally, we discuss the effects of CCN5 expression on yet another aggressive breast cancer subtype, characterized by HER2 overexpression. (ku.edu)
HSP70-2 expression was examined in 154 tumor and 103 adjacent non-cancerous tissue (ANCT) specimens and breast cancer cell lines (MCF7, BT-474, SK-BR-3 and MDA-MB-231) by RT-PCR, quantitative-PCR, immunohistochemistry, Western blotting, flow cytometry and indirect immunofluorescence. (biomedcentral.com)
In the present study, we have investigated the possible association of HSP70-2 mRNA and protein expression with various stages, grades and histotypes of breast cancer patients. (biomedcentral.com)
In the present study, we investigated the expression profile and prognostic potential of EIF5B in HCC tissues and cell lines. (techscience.com)
The study exhibited that this expression of UCK2 mRNA were substantially reduced in treated HT-29 cells. (nanoker-society.org)
In addition, downregulation in expression of 18S ribosomal RNA was also observed in all treated HT-29 cells. (nanoker-society.org)
This was confirmed by fluorescence imaging to measure the level of expression of 18S ribosomal RNA in live cell images. (nanoker-society.org)
Flow cytometry showed that forced expression of miR-208b increased the percentage of cells at the S phase and decreased the percentage of cells at the G0/G1 phase. (engreen.com.cn)
Activation of the VEGFA/VEGFR2 signaling and expression of blood vascular endothelial cell (BEC) markers, such as NRP1 and EphrinB2, further differentiate these precursor cells into BECs, which then form the hierarchical network of blood vessels 4 . (nature.com)
First, expression of SigD R468A caused accumulation of large unbudded cells and loss of septin organization, while SigD expression caused none of these effects. (microbiologyresearch.org)
In that context, differentiated enterocytes in villi exhibit higher guanylin expression and ligand-dependent cGMP accumulation compared to proliferating progenitor cells in crypts. (jefferson.edu)
In this compiled work, we elaborate on the effect of protein expression alterations and their resulting effect on recurrence capability of cells. (lu.se)
To examine its role in cells, our RNA-seq analysis showed that the expression of S970D causes decreased expression of MOV10 enhanced Cross-Linking Immunoprecipitation (eCLIP) targets compared to WT. (cdc.gov)
A Syntenic Cross Species Aneuploidy Genetic Screen Links RCAN1 Expression to β-Cell Mitochondrial Dysfunction in Type 2 Diabetes. (lu.se)

Evidently, CCN5-mediated restoration of ER-α and its downstream signaling cascades renders the TNBC cells sensitive to tamoxifen. (ku.edu)

Tumour suppressor protein, p53, prevents cancer development by eliminating cells with mutagenic alterations or potential for neoplastic transformation or blocking their cell cycle permanently or by transient DNA repair [3C5]. (nanoker-society.org)
The overexpression of Aurora B has been observed in several tumour types, and has been linked with a poor prognosis for cancer patients. (irdrjournal.com)
In this communication we report the synthesis of a new series of chalcones and their corresponding flavanones as well as their antiproliferative activity against the human tumour cell line U-937. (ulpgc.es)

The transferred, expanded natural killer cells proliferated in vivo in an interleukin-2 dose-dependent fashion, persisted up to 4 weeks, were readily detectable in the human bone, inhibited myeloma growth and protected bone from myeloma-induced osteolysis. (haematologica.org)
But these treatments, to some extent, easily produce side effects on normal cells, organs, and other tissues of the human body, thus accelerating the death process of cancer patients. (hindawi.com)
Choline kinase (ChoK) is increased in human mammary tumors with high incidence, and this activation is associated with clinical variable indicators of greater malignancy. (aacrjournals.org)
Synthetic flavanone was as cytotoxic as the antitumor agent etoposide against human leukaemia cells, but human peripheral blood mononuclear cells were more resistant than leukaemia cells to the cytotoxic effects of the flavanone. (ulpgc.es)
We determine a comprehensive map of lineage-specific lncRNAs in human dermal lymphatic and blood vascular endothelial cells (LECs and BECs), combining RNA-Seq and CAGE-Seq. (nature.com)
The PPS1 protein was expressed in Escherichia coli, and the protein was shown to catalyze the hydrolysis of p-nitrophenyl phosphate, dephosphorylate phosphotyrosyl, and phosphothreonyl residues in synthetic diphosphorylated peptides and to inactivate the human ERK1 protein. (embl.de)
2. Gao, Y. et.al: A Cdc2-related protein kinase hPFTAIRE1 from human brain interacting with 14-3-3 proteins. (signalchem.com)
Here we report that human hnRNPA1 physically interacts with the nucleoprotein (NP) of IAV in mammalian cells at different time points of the viral replication cycle. (cdc.gov)
Daneshpajooh M, Bacos K, Bysani M, Bagge A, Ottosson Laakso E, Vikman P, Eliasson L, Mulder H, Ling C. HDAC7 is overexpressed in human diabetic islets and impairs insulin secretion in rat islets and clonal beta cells. (lu.se)
E7 from human papil omavirus type 16 cooperate to cancer: the European prospective investigation into doi:10.1093/carcin/bgp321 PMID:20047954 target the PDZ protein Na/H exchange regulatory cancer and nutrition study. (who.int)

Incubation of cell lysates and detection antibody on the coated microwell plate forms a sandwich with phospho-Aurora A (Thr288) in a single step. (cellsignal.com)

Wound healing assays revealed that migration of MDA-MB-231 hpshRNA cells was markedly impaired compared to MDA-MB-231 mpshRNA cells, especially when cycloheximide was added to block protein synthesis. (spandidos-publications.com)

Conventionally, lung cancers have been divided into non-small-cell lung cancer (NSCLC) and small-cell lung cancer (SCLC). (biomedcentral.com)
Single drugs may cure selected cancers (eg, choriocarcinoma, hairy cell leukemia). (msdmanuals.com)

Overexpression of PFTK1 predicts resistance to chemotherapy in patients with oesophageal squamous cell carcinoma. (signalchem.com)

It has been reported that BMP promotes the self-renewal of both embryonic and somatic stem cells and that BMP signaling activity significantly decreases with age, thus we posit that BMP induction may improve the function of MDSCs. (pitt.edu)
Somatic cells are usually characterized by the absence of telomerase activity. (actanaturae.ru)

Overexpression of this gene product may be associated with neoplastic transformation for some tumors. (wikipedia.org)

It may play an important role in the pathogenesis of allergic diseases such as asthma, and may also have a role in controlling cell growth and/or differentiation. (cancerindex.org)
In this study, highly purified, flow-cytometry sorted, classified in relation to normal B-cell differentiation [1]. (lu.se)

1 Significant advances have been made by combining novel agents with autologous peripheral blood stem cell transplantation which allows for long-term disease-free survival in the majority of transplant-eligible patients. (haematologica.org)

Some new promising strategies for biomarker discovery include microarray-based profiling at the DNA and mRNA levels, and mass-spectrometry-based profiling at the protein and peptide levels [ 2 ]. (biomedcentral.com)

Anatomy13

Organisms11

Diseases15

Chemicals and Drugs130

Analytical, Diagnostic and Therapeutic Techniques and Equipment25

Phenomena and Processes58

Disciplines and Occupations1

Information Science3

Health Care1

Inhibitor6

Inhibitors9

Proliferation37

Apoptosis14

Progression13

Mitogen-activa1

Serine7

Cyclin9

Anti-apoptotic1

Vitro10

Pathway7

Arrest8

Induces5

Phosphorylation2

Mutations6

Inhibition5

PI3K2

Enzyme6

Multiple freeze-thaw cycles2

Phosphatidylinositol1

CDNA library1

Mice2

Budding yeast1

Degradation1

Inhibits2

Cyclins1

Endogenous2

Receptor5

Inactivation3

Chromosome1

Transcription5

Genes4

Senescence3

Phenotype2

Checkpoint1

Regulation9

Pathways5

Mechanisms4

Tyrosine2

Differentially2

Tumor suppressor protein2

Eukaryotic2

Mitosis2

Mantle Cell Lym3

Membrane9

Adhesion4

Cancer Cells16

Cellular6

Mesenchymal1

Molecular3

Expression20

Downstream1

Tumour3

Human10

Lysates1

Migration1

Cancers2

Resistance1

Somatic2

Gene product1

Differentiation2

Survival1

MRNA1