• This study is to investigate the role of p38 mitogen-activated protein kinase (p38MAPK) in tert -butyl hydroperoxide ( t BHP)-induced apoptosis of human trabecular meshwork (iHTM) cells. (molvis.org)
  • Apoptosis signal-regulating kinase 1 (ASK1) is a ubiquitously expressed mitogen-activated protein kinase kinase kinase that activates the c-Jun N-terminal kinase (JNK) and p38 mitogen-activated protein kinase signaling cascades. (nih.gov)
  • Extracellular signal-regulated kinase (ERK) is a part of the mitogen-activated protein kinase (MAPK) signaling pathway which allows the transduction of various cellular signals to final effectors and regulation of elementary cellular processes. (mdpi.com)
  • The secretion of inflammatory factors as well as the marker proteins in the mitogen-activated protein kinase (MAPK) pathway were detected by Western blot. (frontiersin.org)
  • This effect was mediated through the mitogen activated protein kinase (MAPK) signalling pathway. (edu.au)
  • Notably, inhibition of the CaMKK (calmodulin-dependent protein kinase kinase) had little affect on GLUT translocation, whereas the inhibition or knockdown of AMPK (compound C, siRNA) activity prevented GLUT3 translocation to the cell surface after glutamate excitation. (jneurosci.org)
  • Notably, these effects were shown to be mediated, at least in part, via inhibition of the ROS-p38-p53 pathway. (spandidos-publications.com)
  • Inhibition of cell viability was assessed with focus on apoptosis transcriptionally and translationally. (hindawi.com)
  • Consistently, expression of active RAF suppressed apoptosis induced by hBAD and the inhibition of colony formation caused by hBAD could be prevented by RAF. (uni-wuerzburg.de)
  • Targeted inhibition of individual kinases of the MAPK signaling pathway using synthetic compounds represents a promising way to effective anti-cancer therapy. (mdpi.com)
  • First, Tip60 can inhibit the Mdm2-mediated conjugation of the Nedd8 protein to p53, thereby possibly releaving it from transcriptional inhibition by Mdm2. (uni-marburg.de)
  • This ERK5-SUMOylation and subsequent inhibition of ERK5 transactivation is one of the mechanisms to explain diabetes-mediated endothelial inflammation and dysfunction (Circ. (mdanderson.org)
  • Thus, inactive p53 is not associated with a susceptible cytotoxicity of Polo-like kinase 1 inhibition and could rather foster the induction of polyploidy/aneuploidy in surviving cells. (oncotarget.com)
  • In addition, compared to the mono-treatment, combination of Polo-like kinase 1 inhibition with anti-mitotic or DNA damaging agents boosts more severe mitotic defects, effectually triggers apoptosis and strongly inhibits proliferation of cancer cells with functional p53. (oncotarget.com)
  • Activation of AMPK by low energy supply and phosphorylation of LKB1 results in an inhibition of mTOR , thus decreasing energy-consuming processes, in particular translation and, thus, cell growth. (sdbonline.org)
  • In 2004, Dmitry published a series of articles regarding the Wip1 phosphatase inhibition of tumorigenesis through p38 MAP kinase , and after establishing his lab in 2004 at IMCB in Singapore, he continued interrogating the role of Wip1 phosphatase in cancer but also in aging . (lifeboat.com)
  • Furthermore, the mixed inhibition of MEK and IKK led to decreased IL-6 mRNA appearance, synergistic lack of cell viability and elevated apoptosis. (phytid.org)
  • These outcomes demonstrate the electricity of 497839-62-0 IC50 MIB/MS as an instrument to recognize dysregulated kinases also to interrogate kinome dynamics as cells react to targeted Rabbit Polyclonal to ACVL1 kinase inhibition. (phytid.org)
  • a little molecule ATP-competitive inhibitor of BCR-Abl, is an efficient front-line treatment for CML and has generated the idea of targeted kinase inhibition being a viable technique for tumor therapy [2]. (phytid.org)
  • Combined Aurora kinase and EGFR targeting using a monoclonal anti-EGFR antibody was more effective compared to single EGFR and Aurora kinase inhibition. (oncotarget.com)
  • Inhibition of DNA-dependent protein kinase catalytic subunit by small molecule inhibitor NU7026 sensitizes human leukemic K562 cells to benzene metabolite-induced apoptosis. (cdc.gov)
  • Mitogen-activated protein kinases (MAPKs) comprise a large family of proteins activated by a wide range of proinflammatory cytokines and environmental stress. (molvis.org)
  • We also observed that ASK1DeltaN induced expression of several neuron-specific proteins and phosphorylation of neurofilament proteins, confirming that PC12 cells differentiated into mature neuronal cells by ASK1. (nih.gov)
  • Post-translational modifications (PTMs) of proteins, including phosphorylation, acetylation, ubiquitination, and SUMOylation, can regulate the function of proteins, determine the active state and subcellular location of proteins, and dynamically interact with other proteins related to carcinogenesis and progression ( 17 - 20 ). (frontiersin.org)
  • Interestingly, the only known substrate of RIPK1 is none other than RIPK1 itself: The protein forms dimers, which phosphorylate each other at serine-166, ultimately allowing the protein to hook up with other death-domain containing proteins and trigger apoptosis. (alzforum.org)
  • It interacts with a wide variety of proteins, such as apoptosis antigen Fas, centromere protein C, and transcription factor erythroblastosis virus E26 oncogene homolog 1 (ETS1). (wikipedia.org)
  • In addition, using surface plasmon resonance technique we analyzed the direct consequences of hBAD phosphorylation by RAF with respect to complex formation of BAD with 14-3-3 proteins and Bcl-XL. (uni-wuerzburg.de)
  • This pore-forming capacity is dependent on phosphorylation status and interaction with 14-3-3 proteins. (uni-wuerzburg.de)
  • Chief among the intracellular signals is the phosphorylation of proteins belonging to the pocket protein family, such as p107, p130 and retinoblastoma protein (Rb). (bdbiosciences.com)
  • Proteins kinases play essential jobs in oncogenic signaling and so are a major concentrate in the introduction of targeted tumor therapies. (phytid.org)
  • Multiple systems of mobile level of resistance to imatinib have already been described you need to include BCR-Abl-dependent systems such as proteins overexpression or appearance of inhibitor-resistant mutations in the BCR-Abl kinase site, like the T315I gatekeeper mutation [6]. (phytid.org)
  • The manifestation and activation condition of proteins kinases (i.e., the kinome) may contribute considerably towards the mobile adaptation to medication level of resistance, and recent systems have been created to review the kinome retinoic acidity (ATRA) (Physique S7). (phytid.org)
  • Lastly, hydroquinone and NU7026 exhibited synergistic effects on promoting apoptosis by increasing the protein levels of pro-apoptotic proteins Bax and caspase-3 but decreasing the protein expression of anti-apoptotic protein Bcl-2. (cdc.gov)
  • In addition, a siRNA-mediated knockdown of GLUT3 expression was found to sensitize CGNs to glutamate excitation. (jneurosci.org)
  • PTPN2 knockdown exacerbated type I IFN-induced apoptosis in INS-1E, primary rat, and human β-cells. (diabetesjournals.org)
  • Of note, both Bim and JNK1 knockdown protected β-cells against IFN-induced apoptosis in PTPN2-silenced cells. (diabetesjournals.org)
  • Knockdown of P2Y12 promotes IL-1β -induced apoptosis. (researchsquare.com)
  • Immunoblot analysis after siRNA-mediated knockdown of the HTATIP gene (which encodes Tip60) expression showed that the cleavage of the poly(ADP-ribose) polymerase (PARP) upon UV irradiation was reduced, suggesting an impaired apoptotic response. (uni-marburg.de)
  • Instead, Tip60 knockdown led to reduced phosphorylation of histone 2AX (H2AX) and jun-N-terminal kinase (JNK), suggesting a role of Tip60 in the immediate early response to UV light exposure through JNK. (uni-marburg.de)
  • Functional knockdown of ASMase with either the chemical inhibitor, desipramine, or siRNA markedly reduces α-TEA-induced cell surface membrane accumulation of ceramide and its co-localization with Fas, DR5, and FADD, cleavage of caspases-8 and -9 and apoptosis, suggesting an early and critical role for ASMase in α-TEA-induced apoptosis. (biomedcentral.com)
  • Inhibiting Lyn with dasatinib or by shRNA-mediated knockdown decreased the phosphorylation of MEK and IKK. (phytid.org)
  • Role of PDL-1 in NSCLC chemoresistance was assessed using CRISPR/Cas9 knockdown and various functional assays including MTT, cell invasion, sphere formation, and cell apoptosis. (cdc.gov)
  • The present study evaluated the protective effects of PF on UV‑induced skin damage in vitro, and demonstrated that the effects were mediated via the ROS‑p38‑p53 pathway. (spandidos-publications.com)
  • The present findings suggest that local IFN production may interact with a genetic factor ( PTPN2 ) to induce aberrant proapoptotic activity of the BH3-only protein Bim, resulting in increased β-cell apoptosis via JNK activation and the intrinsic apoptotic pathway. (diabetesjournals.org)
  • Wnt/β-catenin signaling is a delicate and complex signal transduction pathway mediated by multiple signaling molecules, which plays a significant role in regulating human physiology and pathology. (frontiersin.org)
  • This suggests the possibility that strictly regulated self-renewal mediated by Wnt signaling in cancer cells may be disturbed by the SUMOylation pathway to allow more malignant proliferation. (frontiersin.org)
  • Silibinin considerably reduced the development of oral cancer cells by inducing apoptosis, G 0 /G 1 arrest, ROS generation, and activation of the JNK/c-Jun pathway. (jcancer.org)
  • After Fas stimulation, Daxx is activated and plays its role of pro-apoptotic protein in activating the c-JUN-N-Terminal Kinase (JNK) pathway. (wikipedia.org)
  • When the cell is treated with TGF-β, HIPK2, a nuclear kinase, phosphorylates Daxx and the activated Daxx in turn activates the JNK pathway (see "The Daxx Pathway" figure). (wikipedia.org)
  • The phosphatidyl inositol 3-kinase (PI3K)/Akt pathway was involved in this ubiquitin-proteasome-mediated degradation of PDCD4. (plos.org)
  • Pulsatile shear stress induces ubiquitin-proteasome-mediated degradation of PDCD4 via a PI3K/Akt pathway in HUVECs. (plos.org)
  • 2002 ). As a pivotal biochemical cascade, the MAPK signaling pathway is involved in diverse fundamental events in cells and signal transduction pathways, namely, differentiation, proliferation, apoptosis, activation of other specific regulatory factors, acute responses to hormones, and developmental alteration of organisms (Pearson et al. (frontiersin.org)
  • Moreover, Tip60 apparently is required for the proper activation of the UV-induced JNK-signaling pathway, thereby ensuring an efficient response to DNA damage and apoptosis induction. (uni-marburg.de)
  • In the present work, we investi-gated the pathway involved in integrin-mediated activation of RON. (123dok.org)
  • The final publication is available at Springer via http://dx.doi.org/10.1007/s12640-010-9207-x Chapter 4 appears to be the equivalent of the post-print version of an article published as: Musgrove RE, King AE, Dickson TC (2013) ˜í¬±-synuclein protects neurons from apoptosis downstream from free radical production through modulation of the MAPK signaling pathway, Neurotoxicity research, 23(4), 358-369. (edu.au)
  • Losmapimod effectively improved memory, reduced the frequency of seizures, protected the neuron from damage, and limited the apoptosis of neurons in epilepsy rats. (frontiersin.org)
  • The data demonstrates that mitochondrial binding of HKII is a dynamic and adaptive response mediated by protein kinase B (PKB)/Akt kinase dependent phosphorylation, that protects neurons from OGD induced apoptosis. (fu-berlin.de)
  • The work in Dr. Strochlic's lab is broadly centered on understanding certain protein kinase-mediated cell signaling pathways in the context of normal development and tumorigenesis. (drexel.edu)
  • TBK1 inhibits RIPK1 by phosphorylating the kinase. (alzforum.org)
  • Inhibits I κ B- α phosphorylation and NF κ B activation in HeLa cells. (tocris.com)
  • Inhibits NF κ B-mediated proinflammatory gene expression in human airway epithelial cells. (tocris.com)
  • Furthermore, his next study showed that diabetes activates p90RSK, which directly inhibits the transcriptional activity of ERK5, an atheroprotective non-classical MAP kinase. (mdanderson.org)
  • p53 represses the promoter of Polo-like kinase 1, whereas Polo-like kinase 1 inhibits p53 and its family members p63 and p73 in cancer cells lacking functional p53. (oncotarget.com)
  • Protein kinases are key regulators of signal transduction pathways that mediate cell growth, proliferation, and motility. (drexel.edu)
  • UV-B-induced DNA damage can trigger cell signaling pathways to activate defense systems, leading to DNA repair and/or apoptosis ( 6 ). (spandidos-publications.com)
  • A multitude of pathways control cyclin-dependent kinase activities as the major event for cell cycle progression. (benthamscience.com)
  • Many of the pro-inflammatory pathways activated during cell death occur upon mitochondrial outer membrane permeabilization (MOMP), the pivotal commitment point to cell death during mitochondrial apoptosis. (nature.com)
  • Caspases, while dispensable for cell death during mitochondrial apoptosis, inhibit activation of pro-inflammatory pathways after MOMP. (nature.com)
  • 2 Apoptosis is triggered by caspase-mediated signal transduction pathways. (bdbiosciences.com)
  • Collectively, our data suggest that venetoclax resistance can be mediated through the enrichment of distinct gene signatures and cytokine signaling pathways. (lu.se)
  • α-TEA induces apoptosis via activation of extrinsic death receptors Fas (CD95) and DR5, JNK/p73/Noxa pathways, and suppression of anti-apoptotic mediators Akt, ERK, c-FLIP and survivin in breast, ovarian and prostate cancer cells. (biomedcentral.com)
  • In this study, we used a new, small molecule, a selective inhibitor of DNA-PKcs, 2-(morpholin-4-yl)-benzo[h]chomen-4-one (NU7026), as a probe to analyze the molecular events and pathways in hydroquinone-induced DNA DSB repair and apoptosis. (cdc.gov)
  • PKC is an important signaling molecule in numerous cell pathways, including cell differentiation, proliferation and apoptosis. (medscape.com)
  • PDCD4 induces turnover (proliferation and apoptosis) of HUVECs. (plos.org)
  • While in the heart this modification induces PDE3-ICER feedback loop and subsequent apoptosis (Circ. (mdanderson.org)
  • Upon treatment with Plk1 inhibitors, p53 in tumor cells is activated and induces strong apoptosis, whereas tumor cells with inactive p53 arrest in mitosis with DNA damage. (oncotarget.com)
  • In this study, we demonstrate that α-TEA induces the accumulation of cell surface membrane ceramide, leading to co-localization with Fas, DR5, and FADD, followed by activation of caspases-8 and -9 and apoptosis in human MDA-MB-231 breast cancer cells. (biomedcentral.com)
  • Intracellular lectins and glycan-modifying enzymes mediate autophagy and control host immunity and inflammation. (nature.com)
  • We discuss here the contribution of glycan-lectin interactions to the initiation, execution and resolution of apoptosis and their emerging roles in other cell death programs including autophagy. (nature.com)
  • Mass spectrometry analysis identified treonine 88 (exclusively mediated by B-RAF) and serine 21 on EF1A1 and serine 21 on EF1A2 as B-/ and C-RAF mediated phosphorylation sites. (unina.it)
  • Living up to its ominous acronym, RIPK1, the receptor-interacting serine/threonine-protein kinase 1 unleashes powerful signaling cascades that can fell cells within hours. (alzforum.org)
  • Our results indicate that RAF kinases phosphorylate hBAD in vivo at these established serine residues. (uni-wuerzburg.de)
  • Additionally, we show that the interaction of the C-terminal segment of hBAD with the BH3 domain results in the formation of permanently open pores, whereby the phosphorylation of serine 118 proved to be necessary for effective pore-formation. (uni-wuerzburg.de)
  • In contrast, phosphorylation of serine 99 in combination with 14-3-3 association suppresses formation of channels. (uni-wuerzburg.de)
  • Our results further indicate that RAF kinases and PAK1 effectively phosphorylate BAD at serine 134. (uni-wuerzburg.de)
  • Notably, in the presence of wild type hBAD, co-expression of survival kinases, such as RAF and PAK1, leads to a strongly increased proliferation, whereas substitution of serine 134 by alanine abolishes this process. (uni-wuerzburg.de)
  • Furthermore, we identified hBAD serine 134 to be strongly involved in survival signaling in B-RAF-V600E containing tumor cells and found phosphorylation of this residue to be crucial for efficient proliferation in these cells. (uni-wuerzburg.de)
  • Mitogen-activated protein kinases (MAPKs) consist of a family of protein-serine/protein-threonine kinases followed by proline, prevalent in mammals (Pearson et al. (frontiersin.org)
  • Five members of the Plk family have been discovered in humans and these serine/threonine kinases have emerged as key players by performing crucial functions in the cell cycle, DNA damage response and neuron biology [ 2 - 6 ]. (oncotarget.com)
  • Further, STAT1 plays an important role in mediating cell viability in response to different cell stimuli and pathogen exposure. (thermofisher.com)
  • Methods for detecting apoptosis or dead cells (viability) by cell preparation type. (bdbiosciences.com)
  • Glutamate excitation induced a rapid alteration in the AMP:ATP ratio that was associated with the activation of the AMP-activated protein kinase (AMPK). (jneurosci.org)
  • In summary, our data suggest that the activation of AMPK and its regulation of cell surface GLUT3 expression is critical in mediating neuronal tolerance to excitotoxicity. (jneurosci.org)
  • We have characterized an AMP-mediated activation of AMPK that facilitates the translocation of GLUT3 to the neuronal cell surface after excitation. (jneurosci.org)
  • Activation of p38MAPK plays an important role in t BHP-induced apoptosis of iHTM cells. (molvis.org)
  • BAX translocation, Bim phosphorylation, cytochrome c release, and caspases 9 and 3 activation were measured by Western blot or immunofluorescence. (diabetesjournals.org)
  • A series of signal transduction events starting from the activation of cyclin-dependent kinase 2 (CDK2) to binding of E2F -target transcription to phosphorylation of Rb helps maintain cell-cycle progression. (bdbiosciences.com)
  • Glioblastoma (GBM), a very aggressive and incurable tumor, often results from constitutive activation of EGFR (epidermal growth factor receptor) and of phosphoinositide 3-kinase (PI3K). (sdbonline.org)
  • G 2 /M arrest was associated with DNA damage as indicated by phosphorylation of H 2 A.X at Ser139 and activation of checkpoint kinase 2 (Chk2) in all the three cell lines. (aspetjournals.org)
  • Cycloheximide or Chk2 inhibitor pretreatment abrogated not only activation of Chk2 but also G 2 /M arrest and apoptosis mediated by DIM. (aspetjournals.org)
  • Blocking Chk2 activation by DN-Chk2 completely protected cells from DIM-mediated G 2 /M arrest. (aspetjournals.org)
  • Moreover, blocking Chk2 activation also abrogates the apoptosis-inducing effects of DIM. (aspetjournals.org)
  • Taken together, these studies demonstrate that ASMase activation and membrane ceramide accumulation are early events contributing to α-TEA-induced apoptosis in vitro and perhaps in vivo . (biomedcentral.com)
  • MIB/MS and immunoblotting verified the over-expression and activation of Lyn in MYL-R cells and determined extra kinases with an increase of (MEK, ERK, IKK, PKC, NEK9) or reduced (Abl, Package, JNK, ATM, Yes) great quantity or activity. (phytid.org)
  • For example, hyper-activation of Lyn or Hck continues to be connected with imatinib level 497839-62-0 IC50 of resistance in CML individuals and cell tradition versions [21]C[24], albeit the systems where these kinases donate to imatinib level of resistance isn't well understood. (phytid.org)
  • Moreover, hydroquinone increased the phosphorylation of Akt to activate Akt, whereas co-treatment with NU7026 prevented the activation of Akt by hydroquinone. (cdc.gov)
  • Previously, we have shown in a mouse model that exposure to hog dust extract (HDE) collected from a CAFO results in the activation of protein kinase C (PKC), elevated lavage fluid cytokines/chemokines including interleukin-6 (IL-6), and the development of significant lung pathology. (cdc.gov)
  • To further analyze the regulation of hBAD function, we investigated the role of these newly identified phosphorylation sites on BAD-mediated apoptosis. (uni-wuerzburg.de)
  • Collectively, our findings provide new insights into the regulation of hBAD function by phosphorylation and its role in cancer signaling. (uni-wuerzburg.de)
  • Unfortunately, the components of CRE-mediated gene regulation may prove to be too complex to be dissected in mammalian systems. (grantome.com)
  • Boosani CS, Mannam AP, Cosgrove D, Silva R, Hodivala-Dilke KM, Keshamouni VG, Sudhakar A. Regulation of COX-2 mediated signaling by alpha3 type IV noncollagenous domain in tumor angiogenesis. (famri.org)
  • In order to investigate the role of HKII in neuroprotective signaling, site directed mutagenesis was used to generate several murine HKII expression vectors with mutations in domains relevant for glucose metabolism and apoptosis regulation. (fu-berlin.de)
  • Recent investigations have shown that the RON receptor is expressed in various cell types including epithelial cells (23) and that MSP-mediated effects on epithelial cells are integrin-dependent (23, 24). (123dok.org)
  • Tight control of the cell cycle in eukaryotic cells exists to control proliferation, differentiation or apoptosis. (benthamscience.com)
  • Differentiation and apoptosis have cell cycle withdraw in common, while cancer and degenerative processes both show altered control of the cell cycle. (benthamscience.com)
  • ASK1 thus appears to mediate signals leading to both differentiation and survival of PC12 cells. (nih.gov)
  • STAT3 protein belongs to a group of intracellular transcription factors that mediate a variety of functions such as cellular differentiation, proliferation, and apoptosis. (biolegend.com)
  • Specify the components and their interactions and set up cluding proliferation, differentiation, apoptosis, embryonic the system of equations. (lu.se)
  • The human trabecular meshwork cells were treated with t BHP for 1 or 2 h with or without pretreatment of SB203580, an inhibitor of MAP kinase homologs. (molvis.org)
  • PS 1145 dihydrochloride is a selective I κ B kinase (IKK) inhibitor (IC 50 = 100 nM). (tocris.com)
  • RAF-induced phosphorylation of hBAD was not prevented by MEK inhibitors but could be reduced to control levels by use of the RAF inhibitor Sorafenib (BAY 43-9006). (uni-wuerzburg.de)
  • Treating SCCHN cell lines with a pan-Aurora kinase inhibitor resulted in defective cytokinesis, polyploidy and apoptosis, which was effective irrespective of the EGFR status. (oncotarget.com)
  • The data showed that adenine activated AMP-activated protein kinase (AMPK) signaling contributing to autophagic cell death through mTOR in both colon cancer cell lines. (hindawi.com)
  • Involvement of AMP-activated protein kinase (AMPK) in anticancer property of adenine was also investigated. (hindawi.com)
  • It phosphorylates and activates several downstream kinases, including AMP-dependent kinase, AMPK . (sdbonline.org)
  • As this interaction establishes a new link between the mitogenic cascade and protein biosynthesis, in vitro kinase assays were performed in presence of B- and C-RAF kinase and recombinant EF1A1/2 to investigate this important interaction. (unina.it)
  • Furthermore, a PDK1-consensus motif is located within the kinase domain of LKB1 and LKB1 gets phosphorylated by PDK1 in vitro. (sdbonline.org)
  • Alpha-tocopherol ether-linked acetic acid (α-TEA), an analog of vitamin E (RRR-alpha-tocopherol), is a potent and selective apoptosis-inducing agent for human cancer cells in vivo and in vitro . (biomedcentral.com)
  • We presently evaluated the mechanisms by which PTPN2 , a candidate gene for T1D, modulates β-cell apoptosis after exposure to type I and II interferons (IFNs), cytokines that contribute to β-cell loss in early T1D. (diabetesjournals.org)
  • The lab uses a combination of genetic, biochemical, and cell biological approaches in both Drosophila melanogaster and mammalian cells to investigate the biology of several different protein kinases. (drexel.edu)
  • Adenine induced significant apoptosis in HT29 cells, whereas Caco-2 cells exhibited less apoptotic responses. (hindawi.com)
  • Inositol hexakisphosphate kinase 2 promotes cell death of anterior horn cells in the spinal cord of patients with amyotrophic lateral sclerosis. (nih.gov)
  • Inositol hexakisphosphate kinase 2 sensitizes ovarian carcinoma cells to multiple cancer therapeutics. (nih.gov)
  • Previous experiments in H1355 cells demonstrated that IFN treatment results in an antiapoptotical answer during which EF1A2 is increasingly expressed due to its RAF kinase mediated phosphorylation. (unina.it)
  • Moreover, it induced cell cycle arrest in the G0/G1 phase, apoptosis, and ROS generation in these cells. (jcancer.org)
  • Several years later, Kerr and co-workers 2 defined the morphological changes in cells undergoing this process, also known as apoptosis. (nature.com)
  • En face co-immunostaining of the mouse aortic arch revealed a low level of PDCD4 in endothelial cells undergoing pulsatile shear stress. (plos.org)
  • Low PDCD4 level was associated with reduced proliferation but not apoptosis or phosphorylation of endothelial nitric oxide synthase caused by pulsatile shear stress to help maintain the homeostasis of endothelial cells. (plos.org)
  • Cells are also programmed for death at the genetic level as a response to DNA damages through the process of apoptosis. (bdbiosciences.com)
  • Apoptosis is an organized process that signals cells to self-destruct for cell renewal or to control aberrant cell growth. (bdbiosciences.com)
  • As cells become damaged or are no longer needed, they undergo apoptosis or programmed cell death, a normal physiological process that occurs during embryonic development and tissue homeostasis. (bdbiosciences.com)
  • Apoptosis controls the orderly death of damaged cells, whereas necrosis occurs as a result of tissue damage, causing the loss of both damaged and surrounding cells. (bdbiosciences.com)
  • Gene set enrichment analysis (GSEA) showed enrichment of cytokine signaling in all three cell lines which was supported by the phospho-kinase array where STAT5 phosphorylation was found to be elevated in resistant cells. (lu.se)
  • In this regard, restoration of p53 in tumor cells with loss or mutation of p53 will reinforce the cytotoxicity of combined Polo-like kinase 1 therapy and provide a proficient strategy for combating relapse and metastasis of cancer. (oncotarget.com)
  • To further establish the involvement of Chk2 in DIM-mediated G 2 /M arrest, cells were transfected with dominant-negative Chk2 (DN-Chk2). (aspetjournals.org)
  • Blocking ROS generation by N -acetyl cysteine protects the cells from DIM-mediated G 2 /M arrest and apoptosis. (aspetjournals.org)
  • Prevention of raft reorganization into larger platforms by inactivation of acid sphingomyelinase (ASMase), renders cells resistant to Fas clustering and apoptosis [ 9 ], indicating that ASMase/ceramide plays an important role in death receptor-mediated apoptosis. (biomedcentral.com)
  • In PEL cells, nigericin disrupted the mitochondrial membrane potential and caused the release of cytochrome c, which triggered caspase-9-mediated apoptosis. (bvsalud.org)
  • ATRA-differentiated HL-60 cells show level of resistance to cytotoxic medicines and loss of life receptor-mediated cell loss of life, and have improved Lyn manifestation which is necessary for their success [64]C[67]. (phytid.org)
  • Bae KM, Wang H, Jiang G, Chen MG, Lu L, Xiao L. Protein kinase C epsilon is overexpressed in primary human non-small cell lung cancers and functionally required for proliferation of non-small cell lung cancer cells in a p21/Cip1-dependent manner. (famri.org)
  • Chemical genetics reveals the requirement for Polo-like kinase 1 activity in positioning RhoA and triggering cytokinesis in human cells. (famri.org)
  • We have previously reported that exposure of workers to benzene and to benzene metabolite hydroquinone in cultured cells induced DNA-dependent protein kinase catalytic subunit (DNA-PKcs) to mediate the cellular response to DNA double strand break (DSB) caused by DNA-damaging metabolites. (cdc.gov)
  • A study by White et al (2009) found that psychosine's cytotoxic effects on oligodendroglia and Schwann cells was mediated through disruption of the architecture and composition of lipid rafts (cell membrane regions characterized by high cholesterol and sphingolipid concentration), followed by altered protein kinase C (PKC) function. (medscape.com)
  • PKC isozymes are LR-dependent molecules that link psychosine-induced LR disruption to reduced PKC function and altered cell signaling activity, possibly driving demyelination and apoptosis in oligodendrocytes and Schwann cells. (medscape.com)
  • Mechanistically, an upregulation of PDL-1 in chemotherapy-treated CAFs led to an increase in hepatocyte growth factor (HGF) secretion, which stimulates cancer progression, cell invasion, and stemness of lung cancer cells, while inhibiting apoptosis. (cdc.gov)
  • Sure enough, they found that the mice were born healthy if one or both copies of the RIPK1 gene were replaced by a kinase-dead version. (alzforum.org)
  • Hence, MIB/MS analysis determined MEK and IKK as essential downstream goals of Lyn, recommending that co-targeting these kinases might provide a distinctive technique to inhibit Lyn-dependent imatinib-resistant CML. (phytid.org)
  • Receptor-mediated tobacco toxicity: alterations of the NF-kappaB expression and activity downstream of alpha7 nicotinic receptor in oral keratinocytes. (famri.org)
  • both EGFR and c-Src kinase activities. (123dok.org)
  • We analyzed the clinical data and performed immunohistochemistry for Epidermal growth factor receptor (EGFR) and Aurora kinase A (Aurora-A) expression in 180 SCCHN patients. (oncotarget.com)
  • Comparing pan-Aurora kinase and Aurora-A targeting hints towards a strong and clinically relevant biological effect mediated via Aurora kinase B. Taken together, our findings characterize a new poor risk group in SCCHN patients defined by elevated EGFR and Aurora-A protein expression. (oncotarget.com)
  • Our results demonstrate that combined targeting of EGFR and Aurora kinases represents a therapeutic means to activate cell cycle checkpoints and apoptosis in SCCHN. (oncotarget.com)
  • Phosphorylation of hBAD by active RAF promotes 14-3-3 protein association, whereby the phosphoserine 99 represents the major binding site. (uni-wuerzburg.de)
  • This transport mechanism is phosphorylation dependent. (wikipedia.org)
  • This study reports that LKB1 binds to Phosphoinositide-dependent kinase (PDK1) by a conserved binding motif. (sdbonline.org)
  • Fas, DR5 and their adaptor FADD, as an essential early event in α-TEA induced Fas and DR5 dependent apoptosis. (biomedcentral.com)
  • Alpha-synuclein expression did not affect free radical production but conferred neuroprotection against caspase-dependent apoptosis. (edu.au)
  • Polo-like kinase 1, a pivotal regulator of mitosis and cytokinesis, is highly expressed in a broad spectrum of tumors and its expression correlates often with poor prognosis, suggesting its potential as a therapeutic target. (oncotarget.com)
  • The subcellular localization and function of this protein are modulated by post-translational modifications, including sumoylation, phosphorylation and polyubiquitination. (wikipedia.org)
  • Not limited to ERK5-SUMOylation, Abe's group also worked on various interesting post-translational modifications including Bcr-mediated PPARg phosphorylation (Circ. (mdanderson.org)
  • Very recently Abe's group found that disturbed flow induced SUMOylation of p53 and ERK5, leading to ECs apoptosis and inflammation, respectively. (mdanderson.org)
  • Comparing embryos carrying normal versus inactive RIPK1, they found that the kinase exacted the brunt of its toll in the fetal liver, where it triggered inflammation and apoptosis. (alzforum.org)
  • Ack kinase regulates CTP synthase filaments during Drosophila oogenesis. (drexel.edu)
  • LKB1 itself is a constitutively active kinase, which is regulated by posttranslational modifications and direct binding to phospholipids of the plasma membrane. (sdbonline.org)
  • Launch The constitutively energetic BCR-Abl tyrosine kinase may be the product from the reciprocal translocation of chromosomes 9 and 22 as well as the causative oncoprotein in over 95% of chronic myeloid leukemia (CML) situations [1]. (phytid.org)
  • In the cytoplasm, the encoded protein may function to regulate apoptosis. (wikipedia.org)
  • STAT1 is known to regulate several genes which are involved in cell growth, apoptosis, immune responses, and lipid metabolism. (thermofisher.com)
  • Taken together, the findings reveal a central role of DNA-PKcs in hydroquinone-induced hematotoxicity in which it coordinates DNA DSB repair, cell cycle progression, and apoptosis to regulate the response to hydroquinone-induced DNA damage. (cdc.gov)
  • Dr. Todd Strochlic's research interests include protein kinases, oncogenic signaling, breast cancer and Drosophila oogenesis. (drexel.edu)
  • Most notably, protein kinases are frequently mutated in many human cancers and are often drivers of oncogenic signaling networks. (drexel.edu)
  • The master kinase LKB1 is a key regulator of several cellular processes, including cell proliferation, cell polarity and cellular metabolism. (sdbonline.org)
  • It is proposed that HKII may serve as a molecular switch regulating apoptosis in a glucose concentration depending fashion and may those provide a link between cellular metabolism and survival. (fu-berlin.de)
  • 2002).[supplied by OMIM] It may be important for translation initiation mediated by the HCV IRES under stress conditions, but this result has been debated. (wikipedia.org)
  • These induce extracellular Daxx to translocalize into the cytoplasm following an association with ASK1 (Apoptosis signal-regulating kinase1). (wikipedia.org)
  • Nevertheless, it is not known whether the Fas-receptor stimuli or the ASK1 overexpression are caused by ROS or CRM1 mediated export. (wikipedia.org)
  • Daxx does not activate JNK itself but rather the upstream JNK kinase kinase ASK1. (wikipedia.org)
  • BAD (Bcl-2 antagonist of cell death, Bcl-2 associated death promoter) is a pro-apoptotic member of the Bcl-2 protein family that is regulated by phosphorylation in response to survival factors. (uni-wuerzburg.de)
  • Small interfering RNAs were used to inhibit PTPN2, STAT1, Bim, and Jun NH 2 -terminal kinase 1 (JNK1) expression. (diabetesjournals.org)
  • We propose that Tip60 can act as a selective antagonist to Mdm2-mediated neddylation. (uni-marburg.de)
  • Homeodomain-interacting protein kinase-2 (HIPK2) is a positive regulator of p53 oncosuppressor function. (aacrjournals.org)
  • To explore the underlying mechanisms, the impact of Tip60 on the Mdm2-mediated ubiquitination of p53 was studied. (uni-marburg.de)
  • Using mass spectrometry we identified in this work, besides the established in vivo phosphorylation sites at serines 75, 99 and 118, several novel hBAD phosphorylation sites (serines 25, 32/34, 97, 124 and 134, Chapter 3.1). (uni-wuerzburg.de)
  • Indeed, they found that TAK1 was more active in mouse embryonic fibroblasts (MEFs) from TBK1 knockout mice than in fibroblasts from wild-type controls, suggesting that the kinase compensates for loss of TBK1. (alzforum.org)