Kinase domain mutations penicillin resistance. Medical search. Frequent questions

Next, we observed that Acanthamoeba specifically activates phosphatidylinositol 3-kinase. (asm.org)
Acanthamoeba -mediated brain endothelial cell death was abolished using LY294002, a phosphatidylinositol 3-kinase inhibitor. (asm.org)
These results were further confirmed using brain microvascular endothelial cells expressing dominant negative forms of phosphatidylinositol 3-kinase. (asm.org)
This is the first demonstration that Acanthamoeba -mediated brain microvascular endothelial cell death is dependent on phosphatidylinositol 3-kinase. (asm.org)
Phosphatidylinositol 3-kinase type 2α (PI3KC2α) is an essential member of the structurally unresolved class II PI3K family with crucial functions in lipid signaling, endocytosis, angiogenesis, viral replication, platelet formation and a role in mitosis. (nature.com)
For example, paeonol inhibits proliferation and induces apoptosis of bladder cancer cells in vitro and in vivo by regulating the phosphorylation expression of phosphatidylinositol 3-kinase and protein kinase B (PKB/AKT). (cjphysiology.org)

For example, prostaglandins activate G s -protein-coupled receptors and initiate cAMP- and protein kinase A (PKA)-mediated processes. (jneurosci.org)
The translocation and the current potentiation were abolished in the presence of an A-kinase anchoring protein (AKAP) inhibitor. (jneurosci.org)
This study provides evidence that a switch-protein kinase regulatory network controls availability of σ 66 , the main sigma subunit for transcription in Chlamydia . (plos.org)
In vitro analysis revealed that a putative switch-protein kinase regulator, RsbW, is capable of interacting directly with σ 66 , as well as phosphorylating its own antagonist, RsbV1, rendering it inactive. (plos.org)
The BRAF protein is a member of the Raf family of serine threonine kinases, which are part of the Ras/RAF/MEK/ERK mitogen activated protein kinase (MAPK) signal transduction cascade that controls cell proliferation and survival (Fig. 1 ) [ 6 ]. (thno.org)
This mutation results in a protein that remains in the active confirmation with an 800-fold increased kinase activity compared with its wild-type counterpart, leading to uncontrolled proliferation and growth of cells expressing BRAF V600E [ 7 ]. (thno.org)
Dual specificity phosphatase 3 (DUSP3), a suppressor of multiple protein tyrosine (Tyr) kinases, is decreased in lung cancer tissues. (biomedcentral.com)
DUSP3/VHR, related to the vaccinia virus phosphatase VH1, is initially found to be a phosphatase that dephosphorylates multiple protein tyrosine kinases (PTKs) in vitro [ 21 ]. (biomedcentral.com)
Receptor-interacting protein (RIP) kinases promote the induction of necrotic cell death pathways. (colinsbraincancer.com)
Active mTORC1 phosphorylates the eukaryotic initiation factor 4E-binding protein-1 (4E-BP1) and 40S ribosomal protein S6 kinase 1 (S6K1) to promote protein synthesis [ 3 , 4 ]. (springer.com)
Conversely, during starvation mTORC1 is inhibited and autophagy is induced by AMP-activated protein kinase (AMPK), leading to generation of intracellular nutrients and energy during degradation of non-functional or non-essential organelles or protein aggregates [ 4 , 6 ], in turn contributing to cell survival. (springer.com)
AND-34/BCAR3 (Breast Cancer Anti-Estrogen Resistance 3) associates with the focal adhesion adaptor protein, p130CAS/BCAR1. (molvis.org)
As opposed to other styles of muscles dystrophy, where mutations take place in genes encoding structural protein, calpainopathy was the initial reported kind of dystrophy predetermined by mutations within a gene encoding a proteolytic enzyme. (exposed-skin-care.net)
Furthermore, the deleterious aftereffect of missense mutations can occur not merely from immediate disruption of CAPN3 function(s), but additionally from disruption from the protein's structural integrity, that could affect its intra or intermolecular protein lead and interactions to decreased stability or altered localization. (exposed-skin-care.net)
Cas9 nuclease can be converted into an RNA-guided DNA binding protein (dCas9) via inactivation of its two catalytic domains 12 , 13 and then fused to transcription activation domains. (cdc.gov)
USP7 interacts with PLK1 protein through its PBD domain by catalytic activity. (biomedcentral.com)
USP7 inhibition induces cell apoptosis and cell cycle G2/M arrest, and overcomes taxane resistance by inducing the protein degradation of PLK1, resulting in chromosome misalignment in mitosis. (biomedcentral.com)
eukaryotic translational initiation aspect-2 kinase 3 (Benefit), inositol-requiring enzyme 1 (IRE1) and activating transcription aspect 6 (ATF6) thus activating these protein. (cgp60474.com)

In patients whose lung adenocarcinomas harbor epidermal growth factor receptor (EGFR) tyrosine kinase domain mutations, acquired resistance to the tyrosine kinase inhibitors (TKI) gefitinib (Iressa) and erlotinib (Tarceva) has been associated with a second-site EGFR mutation, which leads to substitution of methionine for threonine at position 790 (T790M). (aacrjournals.org)
More specifically, substrates with certain FYVE domains (named after the first four proteins in this motif, Fab1, YotB, Vac1p, and EEA1) bind PIP, and these pathways have been implicated in vesicular trafficking or receptor-mediated endocytosis ( 5 ). (asm.org)
Nuclear localized HER family receptor tyrosine kinases (RTKs) have been observed in primary tumor specimens and cancer cell lines for nearly two decades. (plos.org)
The current study aimed to map the putative transactivation domains (TADs) of the HER3 receptor. (plos.org)
Brand TM, Iida M, Luthar N, Wleklinski MJ, Starr MM, Wheeler DL (2013) Mapping C-Terminal Transactivation Domains of the Nuclear HER Family Receptor Tyrosine Kinase HER3. (plos.org)
The ErbB/HER family of receptor tyrosine kinases (RTKs) consists of four family members: the epidermal growth factor receptor (EGFR/ErbB1), HER2 (ErbB2/Neu), HER3 (ErbB3), and HER4 (ErbB4). (plos.org)
MST1R (RON) is a trans-membrane receptor tyrosine kinase (RTK), which is part of the c-MET proto-oncogene family. (frontiersin.org)
Upon binding of insulin to the insulin receptor (IR), IRS1 is phosphorylated at several YXXM motifs creating docking sites for the binding of PI3Kp85, which activates AKT kinase. (scielo.br)
2004) Activating mutations in the epidermal growth factor receptor underlying responsiveness of non-small-cell lung cancer to gefitinib. (scielo.br)
2005) Activating mutations in the tyrosine kinase domain of the epidermal growth factor receptor are associated with improved survival in gefitinib-treated chemorefractory lung adenocarcinomas. (scielo.br)
Metz HE and Houghton AM (2011) Insulin receptor substrate regulation of phosphoinositide 3-kinase. (scielo.br)
In addition to binding to the insulin receptor, IRS1 also binds to and transmits signals from the receptors of prolactin, growth hormone (GH), leptin, vascular endothelial growth factor (VEGF), tropomyosin receptor kinase B (TrkB), anaplastic lymphoma kinase (ALK), insulin like growth factor (IGF1), and integrins ( Vuori and Ruoslathi , 1994 Vuori K and Ruoslahti E (1994) Association of insulin receptor substrate-1 with integrins. (scielo.br)
Non-small cell lung malignancies harboring somatic gain-of-function mutations in the epidermal development factor receptor (EGFR) tyrosine kinase domain respond well to treatment with EGFR tyrosine kinase inhibitors (TKIs) including gefitinib and erlotinib. (technuc.com)
The epidermal growth factor receptor (EGFR) is a receptor tyrosine kinase (RTK) commonly targeted for inhibition by anti-cancer therapeutics. (biorxiv.org)
The epidermal growth factor receptor (EGFR) is a HER-family receptor tyrosine kinase (RTK) that is involved in cell signaling in healthy tissue. (biorxiv.org)
Palliative treatment contains chemo- and radiotherapy and even more lately, focusing on therapy, such as skin development element receptor-tyrosine kinase inhibitors (EGFR-TKI) gefitinib, erlotinib, and icotinib. (cancerhugs.com)
Distinct In Vitro and In Vivo Neutralization Profiles of Monoclonal Antibodies Elicited by the Receptor Binding Domain of the Ancestral SARS-CoV-2. (cdc.gov)
Here we produced and characterized a set of mouse monoclonal antibodies (mAbs) specific for the ancestral SARS-CoV-2 receptor binding domain (RBD). (cdc.gov)

T315I mutated BCR-ABL is the main cause of the resistance mediated by imatinib and second generation BCR-ABL inhibitor. (jcancer.org)
Vemurafenib (Vem, PLX4032, Plexxikon/Roche) is a small molecule, non-covalent tyrosine kinase inhibitor primarily directed towards the BRAF V600E mutation. (thno.org)
Due to the introduction of imatinib, a tyrosine kinase inhibitor (TKI), CML patients now benefit from treatment ( 2 ). (spandidos-publications.com)
We also synthesize a library consisting of 70,290 guides targeting all human RefSeq coding isoforms to screen for genes which, upon activation, confer resistance to a BRAF inhibitor. (cdc.gov)

JOA displayed the activity of inhibiting the BCR-ABL and promoted differentiation of not only imatinib -sensitive but also imatinib -resistant cells with BCR-ABL mutation, which could become a potent lead compound to overcome the imatinib -resistant induced by inhibitors of BCR-ABL tyrosine kinase in CML therapy. (jcancer.org)
For most patients with CML, BCR-ABL tyrosine kinase inhibitors (TKIs) have turned the an inevitably fatal disease into a manageable condition. (jcancer.org)

Therefore, imatinib was a paradigm of targeted therapies, which due to that imatinib inhibits the phosphorylation of BCR-ABL then suppress the activation of BCR-ABL tyrosine kinase [ 6 ]. (jcancer.org)
This process leads to the activation of each receptors' tyrosine kinase and the subsequent phosphorylation of tyrosine residues located on their C-terminal tails. (plos.org)
The forskolin-induced current potentiation was greatly reduced in cells transfected with VR-1 mutants carrying point mutations at the predicted PKA phosphorylation sites. (jneurosci.org)
Mutations of Tyr residues on OCLN showed the role of Tyr phosphorylation in regulating OCLN. (biomedcentral.com)
DUSP3 regulated OCLN ubiquitination and degradation through decreasing OCLN tyrosine phosphorylation directly or through suppressing focal adhesion kinase, the OCLN kinase. (biomedcentral.com)

To additionally check out level of resistance to EGFR TKIs, today's study has generated cell lines that are resistant to either gefitinib or erlotinib. (technuc.com)

Tumor cells from patients with acquired resistance were examined for secondary EGFR kinase domain mutations by molecular analyses. (aacrjournals.org)
We sequenced the coding regions surrounding YXXM motifs of IRS1 using tumor samples of 42 NSCLC patients and 40 matching controls and found heterozygote p.S668T mutation in nine of 42 samples and four of nine also had the p.D674H mutation. (scielo.br)
Today's results demonstrate special gene manifestation patterns of EGFR TKI-resistant lung tumor cells using the T790M mutation. (technuc.com)
Mutations in the tumor suppressor genes encoding TSC1 (Hamartin) and TSC2 (Tuberin) cause a multisystemic tumor syndrome termed tuberous sclerosis complex (TSC). (springer.com)
Lung tumor stem cells are a subpopulation of cells important for lung tumor development, metastasis, and medication resistance. (immune-source.com)
The correlation between USP7 level of tumor tissues and taxane-resistance was evaluated. (biomedcentral.com)

Phosphatidylinositol 3-kinases (PI3Ks) are important signaling molecules that phosphorylate the 3′ OH position of the inositol ring of phosphoinositides (PIs), generating the second messengers PI(3)P, PI(3,4)P 2 , and PI(3,4,5)P 3 ( 4 , 17 ). (asm.org)
Phosphoinositide 3-kinases (PI3Ks) are a family of lipid-modifying enzymes that phosphorylate the 3′-OH group of inositol phospholipids and play key roles in physiology ranging from cell growth and metabolism to organismal development. (nature.com)

Gene ontology (GO) analysis suggested platelet-derived growth factor A (PDGFA) and proto-oncogene, Serine/Threonine kinase Raf-1 (RAF1) as new CRC candidate markers in CTCs and TDEs. (researchsquare.com)

All NSP proteins contain an NH 2 -terminal SH2 (Src homology domain 2) domain, a central proline/serine-rich domain, and a COOH-terminal domain with modest homology to Ras subfamily GDP-exchange factors (GEFs). (molvis.org)

The ligand-bound TM configuration promotes asymmetric dimerization of the intracellular juxta-membrane (JM) and kinase domains, which causes autophosphorylation of intracellular tyrosine residues ( 6 , 7 ). (biorxiv.org)

Here, we determined the effect of Jiyuan oridonin A (JOA), an ent -kaurene diterpenoid compound, on the differentiation blockade in imatinib-sensitive, particularly, imatinib-resistant CML cells with BCR-ABL-T315I mutation by cell proliferation assay, apoptosis analysis, cell differentiation analysis, cell cycle analysis and colony formation assay. (jcancer.org)
To conclude, noise-induced OHC apoptosis and necrosis are modulated by caspases and RIP kinases, respectively. (colinsbraincancer.com)
Long term and extreme ER tension induced -cell apoptosis is certainly connected with c-jun N-terminal kinase (JNK) activation [9], [15]. (cgp60474.com)

This family of RTKs has been highly implicated in the formation and progression of various cancers via aberrant overexpression, kinase activation, and mutation [1] , [2] . (plos.org)
Overexpression of a tandem FYVE domain with high affinity for PtdIns3P alters the shape of the SR cisternae at the triad. (jak-signaling.com)
Expected and potentially novel resistance genes are enriched in the top hits and are validated using individual sgRNA as well as cDNA overexpression. (cdc.gov)

These novel DUSPs have smaller sizes and lack the MAPK-binding domain, in comparison to MKPs. (biomedcentral.com)

The majority of inositol lipids reside in membranes and serve as substrates for kinases, phosphatases, and phospholipases. (asm.org)

Current therapeutics target EGFR's kinase domain or extracellular region. (biorxiv.org)

About 50 % of the situations with acquired level of resistance to EGFR TKIs could be accounted for with a second-site mutation in exon 20 from the EGFR kinase domains, which leads to the substitution of methionine for threonine at placement 790 (T790M) (5,6). (technuc.com)
Additionally, it might be used to recognize genes whose manifestation is transformed in cells Vinflunine Tartrate manufacture with obtained medication resistance by evaluating gene manifestation in drug-resistant cells compared to that in parental cells that are delicate to treatment with, for instance, EGFR TKIs. (technuc.com)

CML with the T315I mutation has been considered to have poor prognosis. (jcancer.org)
We found that JOA at lower concentration significantly inhibited the proliferation of CML cells expressing mutant BCR-ABL (T315I mutation included) and wild-type BCR-ABL, which was due to that JOA induced the cell differentiation and the cell cycle arrest at G0/G1 phase. (jcancer.org)
As a result, cells expressing different forms of BCR/ABL were recruited for the present study, including K562 (human wild‑type) or TCCY‑T315I (human imatinib‑resistant) and the Ba/F3‑(T315I/E279K/Y253H) (mouse BCR/ABL point mutation‑transfected cells). (spandidos-publications.com)
Thus, cells expressing different forms of BCR/ABL were recruited for the present study, including K562 [human wild-type (WT)] or TCCY-T315I [human imatinib-resistant (IR)] and the Ba/F3-(T315I/E279K/Y253H) (mouse BCR/ABL point mutation-tranfected cells). (spandidos-publications.com)

CAPN3 provides some exclusive domains including its NH2-terminal domains I which has 20C30 additional proteins not within - and m-calpains and two exclusive insertion sequences' of 62 and 77 proteins on the COOH-terminal parts of domains II (known as Is normally1) and domains III (known as IS2). (exposed-skin-care.net)
Among the established custom DNA binding domains, Cas9 is most easily scaled to facilitate genome-scale perturbations 3 , 4 due to its simplicity of programming relative to zinc finger proteins and transcription activator-like effectors (TALEs). (cdc.gov)

However, resistance to imatinib occurs in about 20 - 30% of patients with newly diagnosed CML in chronic phase [ 7 ]. (jcancer.org)
However, imatinib resistance has developed as a significant issue. (spandidos-publications.com)

We aimed to elucidate the frequency and nature of secondary EGFR mutations in patients with acquired resistance to TKI monotherapy. (aacrjournals.org)

Furthermore, BRAF gene mutations are present in 30-50% of thyroid cancer, 30% of low-grade ovarian cancers and 15% of colon cancers as well as in a number of other malignancies (totaling 7% of all human cancer diseases) [ 4 ], but additional BRAF-mutated forms are still being identified [ 5 ]. (thno.org)
The most common and best studied mutation of the BRAF gene is the glutamic acid substitution of valine at position 600 (V600E) within the kinase domain. (thno.org)
Today's study can offer crucial insights into gene manifestation profiles involved with conferring level of resistance to EGFR TKI therapy in lung cancers cells. (technuc.com)
Nevertheless, the adjustments of gene appearance involved with EGFR TKI level of resistance because of the T790M mutation stay poorly described. (technuc.com)
Mutations of the signal transducer and activator of transcription 3 (STAT3) gene were shown to cause the AD HIES by 2 groups independently. (medscape.com)
9] Recent progress in immunological research continues to find other gene mutations that can manifest as overlapping clinical features of HIES (high IgE, allergic symptoms, vulnerability to fungal and bacterial infection). (medscape.com)
Increased curiosity about CAPN3 was highly stimulated when it had been reported that mutations in its gene bring about limb-girdle muscular dystrophy (LGMD) type 2A, seen as a the continuous atrophy of hip and make muscle tissues (LGMD2A, or calpainopathy) (21). (exposed-skin-care.net)
You can find over 440 noted mutations within the calpain 3 gene up to now, included in this 212 (50%) are missense mutations, a lot of which alter its catalytic activity (22). (exposed-skin-care.net)

Vemurafenib is clinically approved for the treatment of metastatic and non-resectable melanoma that harbor the BRAF V600E mutation. (thno.org)

However, the resistance to radiotherapy or chemo- is a big issue for lung cancer therapy [1C3]. (immune-source.com)

Results from these analyses demonstrated that the C-terminal domain of HER3 (CTD, amino acids distal to the tyrosine kinase domain) contained potent transactivation potential. (plos.org)

His main research interests concern the identification of genes and enzymes involved in this pathway, the regulation of this metabolism, the mode of action of antibiotics and bacteriocins interfering with this pathway and the corresponding resistance mechanisms, as well as the molecular bases for the recognition of bacterial cell-wall peptidoglycan by host innate immunity mechanisms. (degruyter.com)
Consistently, inactivating mutations ENOblock (AP-III-a4) in various NER genes cause the autosomal recessive syndrome xeroderma pigmentosum (XP), which is Nos1 usually associated with UV sensitivity and susceptibility to skin cancer development (4). (colinsbraincancer.com)
We also reported that inactivating mutations in or of any among other DDR genes mixed up in mobile response to replicative tension cripples NER exclusively in S stage. (colinsbraincancer.com)

We unravel a coincident mechanism of lipid-induced activation of PI3KC2α at membranes that involves large-scale repositioning of its Ras-binding and lipid-binding distal Phox-homology and C-C2 domains, and can serve as a model for the entire class II PI3K family. (nature.com)

nevertheless, individuals who in the beginning react to EGFR-TKI remedies ultimately develop obtained level of resistance. (cancerhugs.com)

These results demonstrate the high resolution with which WGS can aid molecular investigation of diphtheria outbreaks, through the quantification of bacterial genetic relatedness, as well as the detection of virulence factors and antibiotic resistance markers among case isolates. (cdc.gov)

Treatment options are limited at best and drug resistance is common. (frontiersin.org)
Combining reagents with distinct mechanisms of action may be the solution to the problem of drug resistance ( 5 ). (spandidos-publications.com)

To accomplish this goal, various intracellular regions of HER3 were fused to the DNA binding domain of the yeast transcription factor Gal4 (Gal4DBD) and tested for their ability to transactivate Gal4 UAS-luciferase. (plos.org)

The mechanism of resistance has been the subject of numerous studies. (spandidos-publications.com)

Patients suffering from lipodystrophies experience reduced body fat, severe insulin resistance, hypertriglyceridemia, and hypoleptinemia, and nonalcoholic fatty liver disease. (medscape.com)
Obesity is characterized by increased body adiposity and leads to insulin resistance. (medscape.com)

Additionally, several resistance mechanisms, such as BCR-ABL genomic amplification, are considered ( 4 ). (spandidos-publications.com)
Amplification of mutation (9,11) and epithelial to mesenchymal changeover (9,12). (technuc.com)

BCR-ABL oncogene encodes a BCR-ABL tyrosine kinase (TK), which triggers different downstream targets including c-Myc, STAT5 and CrkL, which participated in the control of cell differentiation, proliferation, migration and other cellular behaviors [ 3 - 5 ]. (jcancer.org)

The mechanisms driving the inadequate duration of response have yet to be fully appreciated, and several studies indicate that intrinsic and acquired resistance are the leading culprits [ 10 - 14 ]. (thno.org)

In addition, due to activating mutations, growth signalling and epigenetic dysregulation NRF2 was also found be aberrantly activated in several cancers [ 2 , 3 ]. (hindawi.com)

The enzymes harbour a tuberculosis necrotizing toxin (TNT) domain and are predominately present in pathogenic species. (nature.com)

In the latest years, people discovered that there is certainly a little subpopulation of cells in tumors, which play a essential function in the resistance of cancer to radiotherapy and chemotherapy. (immune-source.com)

This low survival may result from the metastasis of cancer cells and arising resistance to drugs. (oncotarget.com)

All sufferers who knowledge a proclaimed improvement with these medications eventually develop development of disease after a median of a year because of the acquisition of medication resistance (4). (technuc.com)

Puzzling clinical features of AD HIES became better understood following identification of STAT3 mutations as a cause of AD HIES. (medscape.com)

We propose that the resulting disruption of native TM interactions disrupts the conformation of the kinase domain, inhibiting EGFR's ability to send migratory cell signals. (biorxiv.org)
We also showed that the m6A reader YTH domain containing 1 plays an important role in m6A-modified ACADM expression, which is negatively regulated by paeonol, and is involved in A549 cell proliferation and glycolytic reprogramming. (cjphysiology.org)

Conversely, the putative PP2C-like phosphatase domain of chlamydial RsbU was capable of reverting RsbV1 into its active state. (plos.org)

The deubiquitinase USP7 has been identified as an oncogene with key roles in tumorigenesis and therapeutic resistance for a series of cancer types. (biomedcentral.com)

Signaling is then transduced across the membrane by altering the configuration of the transmembrane (TM) domain, by dimerization of the TM helical region or a change in the arrangement of the TM helices within such a dimer. (biorxiv.org)

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