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CortactinPhosphatidylinositol 3-Kinasessrc-Family KinasesMAP Kinase Signaling SystemProtein KinasesProtein-Serine-Threonine KinasesMicrofilament ProteinsProtein Kinase InhibitorsCalcium-Calmodulin-Dependent Protein KinasesActinsActin-Related Protein 3Actin-Related Protein 2PhosphorylationProtein Kinase Cp38 Mitogen-Activated Protein KinasesMitogen-Activated Protein Kinase 1Cyclic AMP-Dependent Protein KinasesProtein-Tyrosine Kinasesp21-Activated KinasesTyrosineMitogen-Activated Protein Kinase 3Mitogen-Activated Protein Kinase KinasesJNK Mitogen-Activated Protein KinasesActin-Related Protein 2-3 ComplexCell Surface ExtensionsWiskott-Aldrich Syndrome Protein, NeuronalProtein BindingCreatine KinasePseudopodiaCDC2 Protein KinaseCyclin-Dependent KinasesMAP Kinase Kinase Kinasessrc Homology DomainseIF-2 KinaseSignal TransductionExtracellular Signal-Regulated MAP KinasesCasein Kinase IICasein KinasesPyruvate KinaseRibosomal Protein S6 KinasesMAP Kinase Kinase 1Receptor Protein-Tyrosine KinasesThymidine KinaseMAP Kinase Kinase 4Focal Adhesion Kinase 1Protein Structure, TertiaryProtein Kinase C-alpha1-Phosphatidylinositol 4-KinasePhosphotransferases (Alcohol Group Acceptor)Mitogen-Activated Protein KinasesCDC2-CDC28 KinasesTransfectionCell LineMolecular Sequence DataFocal Adhesion Protein-Tyrosine KinasesI-kappa B KinaseGlycogen Synthase Kinase 3Proto-Oncogene ProteinsAurora KinasesEnzyme Activationrho-Associated KinasesMyosin-Light-Chain KinaseMutationIsoenzymesProtein Kinase C-deltaDynamin IIProto-Oncogene Proteins pp60(c-src)CytoskeletonAmino Acid SequenceIntracellular Signaling Peptides and ProteinsCell MovementKineticsBlotting, WesternRecombinant Fusion ProteinsCell Line, TumorPhosphoproteinsDiacylglycerol KinasePhosphotyrosineAMP-Activated Protein KinasesCells, CulturedSerineBinding SitesActin CytoskeletonRNA, Small InterferingProtein TransportProto-Oncogene Proteins c-aktAdaptor Proteins, Signal TransducingSubstrate SpecificityBase Sequence3T3 CellsJanus Kinase 2Models, BiologicalRecombinant ProteinsRibosomal Protein S6 Kinases, 90-kDaMicroscopy, FluorescenceProtein Kinase C-epsilonImmunoblottingCytoskeletal ProteinsMAP Kinase Kinase Kinase 1Calcium-Calmodulin-Dependent Protein Kinase Type 2
Tyrosine kinasePhosphorylationProteinsSubstrateCyclin-Dependent KinaseInhibitorProteinSerineSubstratesArp2InvadopodiaWASpExtracellularInteractsPhosphorylatesActin polymerizationCTTNPaxillinInhibitorsFilamentous actinInvasionCytoskeletonReceptorLocalizationMembraneTks5AdhesionPathwaysCytoplasmVitroCellActivityStructuralRegulationFamilyRoleCancerShow
Tyrosine kinase13
  • When a receptor tyrosine kinase on the cell membrane binds to an adhesion site, for example, cortactin will be phosphorylated locally to the area of binding, activate and recruit Arp2/3 to the actin cortex in that region, and thus stimulate cortical actin polymerization and movement of the cell in that direction. (wikipedia.org)
  • and protein tyrosine kinase activity. (nih.gov)
  • and transmembrane receptor protein tyrosine kinase signaling pathway. (nih.gov)
  • Orthologous to human ABL2 (ABL proto-oncogene 2, non-receptor tyrosine kinase). (nih.gov)
  • A Role for the Non-Receptor Tyrosine Kinase Abl2/Arg in Experimental Neuroinflammation. (nih.gov)
  • The Abelson tyrosine kinase (Abl) plays a crucial role in multiple Drosophila axon guidance pathways during development, though the mechanism by which Abl elicits a diverse set of guidance outputs is currently unknown. (silverchair.com)
  • BACKGROUND The Src tyrosine kinase substrate and adaptor proteins Tks5 had previously been implicated in the invasive phenotype of normal and transformed cell types via rules of cytoskeletal constructions called podosomes/invadopodia. (healthanddietblog.info)
  • This Tks5-reliant increase in intrusive behavior additional depended on Src tyrosine kinase activity as well as the phosphorylation of Tks5 at tyrosine residues 557 and 619. (healthanddietblog.info)
  • Src is generally upregulated in advanced stage malignancies and activation of Src tyrosine kinase activity transforms cells to a neoplastic phenotype with improved survival development and migration [8-10]. (healthanddietblog.info)
  • We also focus on determining the mechanism of breast cancer tumor suppression by the Syk tyrosine kinase. (daniel-walz.de)
  • We have identified a novel pathway that directs Lyn/Src family tyrosine kinase signals to the invadopodia to regulate sarcoma cell invasion via the molecule AFAP-1-like-1 (AFAP1L1), a new member of the AFAP (actin filament-associated protein) family. (nature.com)
  • These data define a novel pathway that directs Lyn/Src family tyrosine kinase signals to sarcoma cell invadopodia through specific recruitment of Vav2 and Nck2 to phosphorylated AFAP1L1, to control cell migration and invasion. (nature.com)
  • His Cancer research is multidisciplinary, relying on both Receptor tyrosine kinase, Signal transduction and Tyrosine phosphorylation. (research.com)
Phosphorylation8
Proteins4
  • Other components include signalling molecules such as tyrosine-kinases, GTPases and effectors proteins as in focal adhesion. (u-bordeaux.fr)
  • Invadopodia are small membrane protrusions, rich in actin and actin-binding proteins, such as cortactin and Tks5 3 - 8 . (biorxiv.org)
  • Podosomes are structurally divided into a core, which mainly contains proteins involved in actin polymerization (such as WASP, the Arp2/3 complex and cortactin ), and a surrounding ring populated by integrin receptors and adhesion proteins (for example, paxillin and focal adhesion kinase (FAK/Pyk2) ) [15] . (cellmigration.org)
  • Mondaca JM, Uzair ID, Castro Guijarro AC, Flamini MI , Sanchez AM. Molecular Basis of LH Action on Breast Cancer Cell Migration and Invasion via Kinase and Scaffold Proteins. (conicet.gov.ar)
Substrate1
  • We use cortactin, a Src substrate, and MT1-MMP, an extracellular matrix-degrading, membrane protein, to identify and study the formation of invadopodia and acquisition of proteolytic activity. (daniel-walz.de)
Cyclin-Dependent Kinase1
  • Estrogen-induced activation of Cdk4 and Cdk2 during G1-S phase progression is accompanied by increased cyclin D1 expression and decreased cyclin-dependent kinase inhibitor association with cyclin E-Cdk2. (research.com)
Inhibitor1
  • Combination Treatment of Retinoic Acid Plus Focal Adhesion Kinase Inhibitor Prevents Tumor Growth and Breast Cancer Cell Metastasis. (conicet.gov.ar)
Protein7
  • Cortactin (from "cortical actin binding protein") is a monomeric protein located in the cytoplasm of cells that can be activated by external stimuli to promote polymerization and rearrangement of the actin cytoskeleton, especially the actin cortex around the cellular periphery. (wikipedia.org)
  • Recently, however, it has been found that dynamin, the protein responsible for breaking the newly formed vesicular bud off the inside of the plasma membrane, can associate with the SH3 domain of cortactin. (wikipedia.org)
  • We found that Homer3, the predominant isoform in Purkinje cells, is phosphorylated by calcium/calmodulin-dependent protein kinase II (CaMKII) both in vitro and in vivo . (jneurosci.org)
  • Consistent with this, mRNA and protein levels of the invadopodia key components, cortactin and MT1-MMP, peak at 14 hours post-release. (biorxiv.org)
  • Cortactin, an actin binding protein, has been associated with Sertoli cell ectoplasmic specializations in vivo , based on its immunolocalization around the heads of elongated spermatids, but not previously identified in isolated Sertoli cells. (biomedcentral.com)
  • Though they talk about a number of the same cytoskeletal regulatory equipment (integrins tyrosine kinases Arp2/3 WASp) as additional adhesion constructions they are recognized by marker protein CK-1827452 (Omecamtiv mecarbil) (cortactin dynamin2 Tks5) and metalloproteinases (MT1-MMP) that distinctively support focalized matrix redesigning activity [2-5]. (healthanddietblog.info)
  • AFAP1L1 is a novel adaptor protein of the AFAP family that interacts with cortactin and localizes to invadosomes. (nature.com)
Serine1
  • Certain serine/threonine kinases, such as ERK, can phosphorylate cortactin on Ser405 and Ser418 in the SH3 domain. (wikipedia.org)
Substrates1
  • Synthetic peptides including acidic clusters as substrates of yeast casein kinase-2. (cnr.it)
Arp27
  • As the concentration of phosphorylated cortactin increases in specific regions within the cell, the monomers each begin to recruit an Arp2/3 complex to F-actin. (wikipedia.org)
  • The ability of these other NPFs to bind the Arp2/3 complex while cortactin is also bound could come from new interactions with cortactin's SH3 domain, which is in a different conformation when phosphorylated by Ser/Thr kinases and thus may be more open to interactions with other NPFs. (wikipedia.org)
  • Having other NPFs bind to the Arp2/3 complex at the same time as cortactin may enhance nucleation site stability. (wikipedia.org)
  • Cortactin-assisted Arp2/3-nucleated actin branches are most prominent in the actin cortex, around the periphery of the cell. (wikipedia.org)
  • A phosphorylated cortactin monomer binds to, activates, and stabilizes an Arp2/3 complex on preexisting F-actin, which provides a nucleation site for a new actin branch to form from the "mother" filament. (wikipedia.org)
  • Since cortactin recruits the Arp2/3 complexes that lead to actin polymerization, this suggests that it may play an important part in linking vesicle formation to the as yet unknown functions actin has in endocytosis. (wikipedia.org)
  • Uzair ID, Flamini MI , Sanchez AM. Rapid Estrogen and Progesterone Signaling to Dendritic Spine Formation via Cortactin/Wave1-Arp2/3 Complex. (conicet.gov.ar)
Invadopodia1
  • AFAP1L1 intersects several invadopodia pathway components through its multiple domains and motifs, including the following (i) pleckstrin homology domains that bind phospholipids generated at the plasma membrane by phosphoinositide 3-kinase, (ii) a direct filamentous-actin binding domain and (iii) phospho-tyrosine motifs (pY136 and pY566) that specifically bind Vav2 and Nck2 SH2 domains, respectively. (nature.com)
WASp1
  • Uzair ID, Conte Grand J, Flamini MI , Sanchez AM. Molecular Actions of Thyroid Hormone on Breast Cancer Cell Migration and Invasion via Cortactin/N-WASP. (conicet.gov.ar)
Extracellular1
  • Cortactin acts as a link between extracellular signals and lamellipodial "steering. (wikipedia.org)
Interacts2
  • ERR-2 suppresses GBM cell migration and interacts with the actin nucleation-promoting aspect cortactin, and an ERR- agonist is able to remodel the actin cytoskeleton and similarly suppress GBM cell migration. (researchreportone.com)
  • Fgd1, the Cdc42 GEF responsible for Faciogenital Dysplasia, directly interacts with cortactin and mAbp1 to modulate cell shape. (medlineplus.gov)
Phosphorylates1
  • The SH3 domain of certain tyrosine kinases, such as the oncogene Src kinase, binds to cortactin's proline-rich region and phosphorylates it on Tyr421, Tyr466, and Tyr482. (wikipedia.org)
Actin polymerization2
  • Cortactin is very active in lamellipodia, protrusions of the cell membrane formed by actin polymerization and treadmilling that propel the cell along a surface as it migrates towards some target. (wikipedia.org)
  • degrees of phosphorylated cortactin (a downstream focus on of Src kinase involved with actin polymerization) had been considerably raised 1 and 3 times post IOP normalization and came back to control amounts by time 5. (ees2010prague.org)
CTTN1
Paxillin1
  • If so, you might anticipate modulation in the experience of varied downstream mediators of integrin receptors inside the ONH upon IOP elevation, including Src and FAK kinases and their downstream goals, cortactin and paxillin, respectively. (ees2010prague.org)
Inhibitors1
  • In recent years, cell cycle regulators have been shown to exhibit roles in both tumor suppression and tumor promotion, particularly cyclin-dependent kinase inhibitors (CKI) p27 kip1 and p21 cip1 14 . (biorxiv.org)
Filamentous actin1
  • Once activated in this way, it can bind to filamentous actin (F-actin) with the fourth of its cortactin repeats. (wikipedia.org)
Invasion2
  • Cortactin promotes cell migration and invasion via upregulation of the dedicator of cytokinesis 1 expression in human colorectal most cancers. (tgf-b1.com)
  • Our research encompasses basic cell biology of tumor cell invasion, focusing on two tyrosine kinases, c-Src and Syk, with opposing roles in cancer metastasis. (daniel-walz.de)
Cytoskeleton1
  • The latter is dependent on a reorganization of the host cytoskeleton (actin/cortactin recruitment), whilst active penetration does not rely on the host's cellular machinery. (fapesp.br)
Receptor3
  • thus, cortactin must also be activated by receptor kinases that pick up a large variety of chemical signals. (wikipedia.org)
  • Integrin receptor activity network marketing leads to immediate activation of a genuine variety of intracellular kinases, like the focal adhesion kinase (FAK) and Src kinase family [32]. (ees2010prague.org)
  • Src may be the namesake person in a grouped category of non-receptor tyrosine kinases as well as the initial described protooncogene [7]. (healthanddietblog.info)
Localization2
  • Double-labeling experiments in HeLa cells invaded with S. flexneri showed a near perfect co-localization of cortactin with actin in the entry structure and at the periphery of the phagosome [ 17 ]. (biomedcentral.com)
  • The presence or absence of FSH had no apparent effect on the distribution or localization of cortactin within Sertoli cells. (biomedcentral.com)
Membrane1
  • Cortactin is recruited to the actin-rich membrane ruffles of the entry structure of Shigella flexneri when invading HeLa cells and is also found in the periphery of the phagosome shortly after internalization [ 17 ]. (biomedcentral.com)
Tks51
Adhesion1
  • Although the presence of cortactin in Sertoli cells has been addressed relative to its role in cell-cell adhesion [ 18 ], its role in phagocytosis has not yet been investigated. (biomedcentral.com)
Pathways1
  • Moreover, we find that Abl exerts its diverse activities through at least two different mechanisms: (1) a partly kinase-independent, structural function in midline attraction through its C-terminal F-actin binding domain (FABD) and (2) a kinase-dependent inhibition of repulsive guidance pathways that does not require the Abl C terminus. (silverchair.com)
Cytoplasm2
  • Cells exposed to no hormone and no beads appeared to have a ubiquitous distribution of cortactin throughout the cytoplasm. (biomedcentral.com)
  • Cortactin immunostaining was diffuse throughout the Sertoli cell cytoplasm when incubated without beads (Fig. 1A ). (biomedcentral.com)
Vitro2
Cell3
  • Thus, polymerization and branching of actin is promoted in areas of the cell where cortactin is localized. (wikipedia.org)
  • Cell cycle progression is governed by a complex network of cyclin-dependent kinases that define not only the phase of the cell cycle, but also the timing of transitions between phases 13 . (biorxiv.org)
  • Oshima T, Fujino T, Ando K, Hayakawa M. Role of FGD1, a Cdc42 guanine nucleotide exchange factor, in epidermal growth factor-stimulated c-Jun NH2-terminal kinase activation and cell migration. (medlineplus.gov)
Activity3
  • The purpose of this study was to identify the presence and distribution of cortactin in isolated rat Sertoli cells active in phagocytic activity following the addition of 0.8 μm latex beads. (biomedcentral.com)
  • FSH exposure did not alter the distribution of cortactin within Sertoli cells, even when phagocytic activity was upregulated by the presence of beads. (biomedcentral.com)
  • Tyr-857 has therefore been assigned a role in positive regulation of kinase activity. (pdgfrsignaling.com)
Structural1
  • These results highlight how a multifunctional kinase can trigger diverse axon guidance outcomes through the use of distinct structural motifs. (silverchair.com)
Regulation1
  • Regulation of MT dynamics via direct binding of an Abl family kinase. (nih.gov)
Family1
  • Dynamic FAK and Src kinase family have been been shown to be essential regulators of mobile responses to damage in cultured astrocytes [33, 34]. (ees2010prague.org)
Role1
Cancer1
  • His Cancer research incorporates themes from Epigenetics and Kinase. (research.com)
Show1
  • In the presence of cytochalasin D, cortactin immunostaining was punctate and distributed in a pattern similar to that reported for actin in cells exposed to cytochalasin D. Sertoli cells not exposed to FSH, but activated with beads, did not show cortactin immunostaining around the phagocytized beads at any of the time periods. (biomedcentral.com)