Killer cells lymphokine activated. Medical search. Frequent questions

The method is used to quantify natural killer (NK) and lymphokine-activated killer (LAK) activities against the human K562 and Daudi cell lines and the murine YAC-1 and L1210 cell lines respectively, as well as cell-mediated lympholysis (CML) exerted by tumor-infiltrating lymphocytes (TIL) against autologous and allogeneic human breast cancer tumor cells. (nih.gov)
6. In vitro induction of tumor-specific human lymphocyte antigen class I-restricted CD8 cytotoxic T lymphocytes by ovarian tumor antigen-pulsed autologous dendritic cells from patients with advanced ovarian cancer. (nih.gov)
7. Enhanced killing of primary ovarian cancer by retargeting autologous cytokine-induced killer cells with bispecific antibodies: a preclinical study. (nih.gov)
16. Human CD4+ effector T cells mediate indirect interleukin-12- and interferon-gamma-dependent suppression of autologous HLA-negative lung tumor xenografts in severe combined immunodeficient mice. (nih.gov)
Coadministration of autologous in vitro activated LAK cells together with IL-2 did not significantly affect the pattern of sCD25 release relative to administration of IL-2 alone. (nih.gov)
The remarkable proliferation of helper T cell subset in response to autologous thyrocytes and intrathyroidal T cells from patients with Graves' disease We have studied cellular interactions among thyrocytes, intrathyroidal T cells and peripheral blood T cells from Graves' patients. (nika-robot.de)

If lymphocytes are cultured in the presence of Interleukin 2, it results in the development of effector cells which are cytotoxic to tumor cells. (wikipedia.org)
It has been shown that lymphocytes, when exposed to Interleukin 2, are capable of lysing fresh, non-cultured cancer cells, both primary and metastatic. (wikipedia.org)
LAK cell therapy is a method that uses interleukin 2 (IL-2) to enhance the number of lymphocytes in an in vitro setting, and it has formed the foundation of many immunotherapy assays that are now in use. (wikipedia.org)
5. Targeting human T-lymphocytes with bispecific antibodies to react against human ovarian carcinoma cells growing in nu/nu mice. (nih.gov)
8. Human tumor antigen-specific T lymphocytes and interleukin-2-activated natural killer cells: comparisons of antitumor effects in vitro and in vivo. (nih.gov)
17. Interferon effect on cytotoxicity of peripheral blood and tumor-associated lymphocytes against human ovarian carcinoma cells. (nih.gov)
After adjusting for covariates, fuel system maintenance personnel (n = 45) were found to have significantly higher counts of white blood cells (P = 0.01), neutrophils (P = 0.05), and monocytes (P = 0.02) when compared with a low-exposure group (n = 78), but no differences were noted in the numbers of total lymphocytes, T-cells, T-helper cells, T-suppressor cells, natural killer cells, and B-cells. (nih.gov)
It is expressed on B lymphocytes at all stages from pro-B through mature and activated B cell, but it is decreased on plasma cells and a subset of memory B cells. (bdbiosciences.com)
The CD45R antigen has been reported not to be on hematopoietic stem cells, naive T lymphocytes, or MHC-restricted CTL. (bdbiosciences.com)
Cross-reaction of the RA3-6B2 clone with activated human T lymphocytes has also been reportedly observed. (bdbiosciences.com)
Natural killer (NK) cells and T lymphocytes are the major effector cells that can recognize and kill tumor cells. (lu.se)
Cytolytic lymphocytes with the unique capacity of killing natural killer (NK)-resistant fresh tumor cells. (nih.gov)
It may be brought about directly by sensitized T-lymphocytes or by lymphoid or myeloid "killer" cells, or it may be mediated by cytotoxic antibody, cytotoxic factor released by lymphoid cells, or complement. (bvsalud.org)
Tumor-infiltrating lymphocytes (TILs) may have greater tumoricidal activity than LAK cells. (msdmanuals.com)
Functional changes in lymphokine-activated lymphocytes depending on the incubation time with interleukin-2]. (ox.ac.uk)
The lymphoid cells commonly used are lymphokine-activated killer (LAK) cells and tumor-infiltrating lymphocytes (TIL). (nih.gov)
Lymphokine-activated killer cells (LAK) are cytolytic lymphocytes with the unique capacity of killing NK-resistant fresh human tumor cells in short-term assays. (studybuff.com)
Tumor-infiltrating lymphocytes can recognize and kill cancer cells. (studybuff.com)
Lymphokines are cytokines produced by T cells (lymphocytes) of the immune system. (studybuff.com)
A type of treatment in which tumor-infiltrating lymphocytes (a type of immune cell that can recognize and kill cancer cells) are removed from a patient's tumor and grown in large numbers in a laboratory. (studybuff.com)
In contrast to T-lymphocytes, these cells lack antigen-specific receptors. (biomedcentral.com)

Careful re-evaluation of the clinical premises and focus on combination and cell therapy may be the way to achieve improved survival by immunotherapy in PC. (medscape.com)
3. Local adoptive immunotherapy of human head and neck cancer xenografts in nude mice with lymphokine-activated killer cells and interleukin 2. (nih.gov)
12. [Adoptive immunotherapy by intra-arterial infusion of ATLAK or Allo-TLAK cells in patients with head and neck cancer]. (nih.gov)
18. Immunotherapy of cancer by systemic administration of lymphoid cells plus interleukin-2. (nih.gov)
1994. Selected immunotherapy studies of the interferons and IL-2/LAK cells. (stjohns.edu)
Additionally, the enhancement effect of 90K in the Dendritic cell (DC) vaccine for Immunotherapy of glioblastoma (GBM) was evaluated in vitro . (aging-us.com)
To improve the tumor-killing efficiency, a combination of tumor-specific antigen and apoptotic GBM tumor cell lysate was pulsed DC to improve the effect of immunotherapy. (aging-us.com)
LAK cells are used for ADOPTIVE IMMUNOTHERAPY in cancer patients. (nih.gov)
In passive cellular immunotherapy, specific effector cells are directly infused and are not induced within the patient. (msdmanuals.com)
Passive cellular immunotherapy references A number of immunologic interventions, both passive and active, can be directed against tumor cells. (msdmanuals.com)
In passive cellular immunotherapy, specific effector cells. (msdmanuals.com)
CAR T cell immunotherapy for human cancer. (msdmanuals.com)
His studies in Cancer research integrate themes in fields like Cell, Lung cancer, Tumor progression and Immunotherapy. (research.com)
Stereotactic ablative radiotherapy with or without immunotherapy for early-stage or isolated lung parenchymal recurrent node-negative non-small-cell lung cancer: an open-label, randomised, phase 2 trial. (viictr.org)
We evaluated the susceptibility of Ewing sarcoma to natural killer cell-based combination immunotherapy, by assessing the capacity of histone deacetylase inhibitors to improve immune recognition and sensitize for natural killer cell cytotoxicity. (biomedcentral.com)
Our data provide a rationale for combination immunotherapy involving immune effector and target cell manipulation in first-/second-line treatment regimens for Ewing sarcoma. (biomedcentral.com)
Manipulation of either the balance between activating and inhibitory signals (by, for instance, e x vivo activation of immune cells) and/or sensitization of target cells for immune-mediated killing by combination immunotherapy may improve immunotherapeutic efficacy. (biomedcentral.com)

Culturing of lymphoid cells with IL-2 preferentially expanded NK cells and induced lymphokine activated killer (LAK). (lu.se)
Culturing of lymphoid cells with IL-2 preferentially expanded NK cells and induced lymphokine activated killer (LAK) activity that preferentially was mediated by IL-2 activated NK cells (NK-LAK) and only to a minor extent by T cells (T-LAK). (lu.se)
Stimulation of lymphoid cells with SEA preferentially expanded T cells expressing certain TCR variable beta elements both in in vitro culture and in vivo following intraperitoneal (i.p.) injections of SEA. (lu.se)
Lymphokines are the products of activated lymphoid cells. (studybuff.com)

Lymphokine activated killer (LAK) cells administered in conjunction with recombinant interleukin-2 can mediate the regression of metastatic tumor in some patients with advanced cancer. (johnshopkins.edu)
Aldesleukin, a lymphokine, is produced by recombinant DNA technology using a genetically engineered E. coli strain containing an analog of the human interleukin-2 gene. (nih.gov)

Activation of murine cytotoxic cells with interelukin-2 and the bacterial superantigen staphylococcal entertoxin A. (lu.se)
Both in vitro and in vivo stimulation with IL-2 and the superantigen, Staphylococcal enterotoxin A (SEA) were used to characterize murine cytotoxic cells functionally and phenotypically. (lu.se)
it can stimulate the body's natural killer, lymphokine-activated killer (LAK) and other cytotoxic cells to destroy malignant cells. (medicallessons.net)
Dive into the research topics of 'Activation of murine cytotoxic cells with interelukin-2 and the bacterial superantigen staphylococcal entertoxin A.'. Together they form a unique fingerprint. (lu.se)

In 98 healthy individuals, FCA with peripheral blood mononuclear cells (PBMCs) at effector to target (E/T) ratio of 32 revealed 46.5 ± 2.6% cytolysis of K562 cells, and 23.5 ± 1.1% of NK cells showed increased degranulation. (hindawi.com)
FCA has several advantages such as discrimination of target cells from effector cells and of dead ones from live target cells, enumeration of NK subsets, and possibility of large number of tests. (hindawi.com)
However, FCA results are largely dependent on test variability, including used target or effector cell type and procedures, and limited data is available for healthy individuals [ 18 - 20 ]. (hindawi.com)
In NK function test, when effector cells are added to growing adherent target cells, the cell index decreases and can be changed into the NK cytotoxicity, as it has been used previously for cytotoxic function of NK cell lines on several tumor cell lines [ 14 ]. (hindawi.com)
The use of the chromium-release assay to determine cytotoxicity of effector against target cells has various limitations mostly due to the inherent properties of the radioactive substance. (nih.gov)
Data analysis is performed by gating the regions of living target, dead target and living effector cells, based on appropriate controls. (nih.gov)
These strategies include eliminating tumor bulk to provoke the release of tumor antigens, using adjuvants to enhance antigen-presenting cell function, and employ agents that enhance immune cell effector activity. (frontiersin.org)
A promising approach to engage effector cells of the immune system overcoming some of the escape mechanisms has been introduced more than two decades ago. (mdpi.com)
The phenomenon of target cell destruction by immunologically active effector cells. (bvsalud.org)
Concomitant use of interferon enhances the expression of major histocompatibility complex (MHC) antigens and TAAs on tumor cells, thereby augmenting the killing of tumor cells by the infused effector cells. (msdmanuals.com)
Natural killer (NK) cells are the main cytotoxic effector cells of the innate immune system, contributing to host anti-microbial and anti-tumour immune responses. (biomedcentral.com)
Learn more about T helper 1 cells (Th1 cells), including T helper 1 cell differentiation, and effector function. (nika-robot.de)

Cell-wall β-glucans from natural sources including plant, fungi, and bacteria are molecules that adopt pathogen-associated molecular pattern (PAMP) known to target specific receptors on immune cell subsets. (frontiersin.org)
Insulin receptors in human circulating cells and fibroblasts. (nih.gov)
Treatments on the horizon are vaccine therapy that spurs the immune system, gene therapy that introduces genes to eradicate malignant cells, and targeted drug therapy that is designed to take advantage of certain specific receptors that are overexpressed on tumor cells. (snmjournals.org)
Chimeric antigen receptors (CAR) recognize specific proteins on the surface of tumor cells. (msdmanuals.com)
Sensitivity of tumours to natural killer cells critically depends on expression of ligands for activating receptors. (biomedcentral.com)

IL-2 stimulates growth and differentiation of B-cells, NK cells, lymphokine-activated killer cells, monocytes, macrophages and oligodendrocytes. (peprotech.com)

Although real-time cell electronic sensing (RT-CES) system-based natural killer (NK) cytotoxicity has been introduced, it has not been evaluated using human blood samples. (hindawi.com)
NK cell activation by adding IL-2 demonstrated real-time dynamic killing activity, and lymphokine-activated PBMC (E/T ratio of 32) from 15 individuals showed 59.1 ± 6.2% cytotoxicity results after 4 hours incubation in RT-CES system. (hindawi.com)
After K562 target cell stimulation, PBMC produced profound proinflammatory and immunoregulatory cytokines/chemokines including IL-2, IL-8, IL-10, MIP-1 α β , IFN- γ , and TNF- α , and cytokine/chemokine secretion was related to flowcytometry-based NK cytotoxicity. (hindawi.com)
They induce direct cytotoxicity or secretion of cytokine/chemokine without recognizing a specific antigen as B and T cells [ 1 ]. (hindawi.com)
NK cytotoxicity plays an important role in immune response against infected cells, malignancy, and stressed cells, and involves in pathologic process in various diseases [ 2 - 5 ]. (hindawi.com)
FCA can also measure the specific NK activation markers in addition to the analysis of target cells cytotoxicity. (hindawi.com)
The real-time cell electronic sensing (RT-CES) system using xCELLigence (Roche Diagnostics, Penzberg, Germany) has been introduced as an alternative for label-free in vitro quantification of NK cell-mediated cytotoxicity [ 14 ]. (hindawi.com)
Immunological effects included the induction of lymphokine-activated killer activity and antibody-dependent cellular cytotoxicity by peripheral blood mononuclear cells. (nih.gov)
T cells responded with induction of profound cytokine production and cytotoxic activity that could be targeted to tumor cells expressing MHC class II, this is termed staphylococcal enterotoxin dependent cell-mediated cytotoxicity (SDCC). (lu.se)
An in-vitro study using taurine in a physiological dose equivalent of 5g daily intravenously measured endothelial cell responses including NK and LAK activity, cytotoxicity, endothelial cell adherence and lymphocyte production. (ndnr.com)
It appears to be implicated in immune response associated with natural killer (NK) and lymphokine-activated killer (LAK) cell cytotoxicity. (nih.gov)
He interconnects Tumor Escape, Fragmentation, Cytotoxicity and Mitochondrion, Mitochondrial fission in the investigation of issues within Cell. (research.com)
Natural killer cell deficiency (NKD) is a primary immunodeficiency where the main defect lies in CD56 + CD3 − natural killer (NK) cells which mediate cytotoxicity against tumors. (studybuff.com)
Natural killer cell cytotoxicity was evaluated in Chromium release assays. (biomedcentral.com)
Histone deacetylase inhibitor-pretreatment induced NKG2D-ligand expression in an ATM/ATR-dependent manner and sensitized for NKG2D-dependent cytotoxicity (2/4 cell lines). (biomedcentral.com)
For example, pre-conditioning of various cancer cell types by agents that activate the DNA damage response pathway may sensitize for natural killer cell cytotoxicity via induction of activating natural killer cell receptor ligands and/or death receptor expression. (biomedcentral.com)

Natural killer (NK) cells are a major component of innate immunity and are responsible for immune surveillance. (hindawi.com)
The innate immune system is the first line of defence against invading pathogens and is particularly important in warding off bacterial and viral infections presenting at the mucosal cell surface. (asai.ie)
Extracellular HMGB1 mediates a number of important functions including endothelial cell activation, stromagenesis, recruitment and activation of innate immune cells, and also dendritic cell maturation that, in the setting of cancer, lead to a chronic inflammatory response. (elsevierpure.com)
His Interleukin 21 research incorporates themes from Natural killer T cell, Innate immune system, Antibody and Hepatitis B virus. (research.com)

These areas include immunology, molecular genetics, endocrinology, and cell biology of breast cancer development. (nih.gov)
His main research concerns Immunology, Interleukin 21, Inflammation, Lymphokine-activated killer cell and Interleukin 12. (research.com)
His work deals with themes such as NKG2D, Stem cell and Natural killer cell, which intersect with Immunology. (research.com)
His primary scientific interests are in Immunology, Immune system, Cancer research, Natural killer cell and Interleukin 21. (research.com)
He works mostly in the field of Immunology, limiting it down to topics relating to Cytotoxic T cell and, in certain cases, CD8 and T cell. (research.com)
Cancer research, Immunology, Cell biology, Immune system and Natural killer cell are his primary areas of study. (research.com)
His work on Immunology is being expanded to include thematically relevant topics such as THP1 cell line. (research.com)
Rui Sun mostly deals with Cancer research, Immunology, Immune system, Immune tolerance and Cell. (research.com)

LAK cells, along with the administration of IL-2 have been experimentally used to treat cancer in mice and humans, but there is very high toxicity with this treatment - Severe fluid retention was the major side effect of therapy, although all side effects resolved after interleukin-2 administration was stopped. (wikipedia.org)
Repetitive weekly cycles of interleukin 2: effect of outpatient treatment with a lower dose of interleukin 2 on non-major histocompatibility complex-restricted killer activity. (nih.gov)
An outbreak of hepatitis A among cancer patients treated with interleukin-2 and lymphokine activated killer cells. (cornell.edu)
Interleukin-2 is the principal growth factor for both NK and T cells. (lu.se)
1995. Improved long-term survival following intracavitary interleukin-2 (IL-2) and lymphokine activated killer cells for adults with recurrent malignant glioma. (stjohns.edu)
1991. Biologic response to intracavitary Interleukin-2/ lymphokine activated killer (LAK) cells in the treatment of primary malignant brain tumors. (stjohns.edu)
1985. Identification of a helper cell activation complex (HAC) expression and its relationship to Interleukin-1 function. (stjohns.edu)
interleukin 12 subunit beta/alpha or IL12b/IL12a is a growth factor cytokine which increases the lytic activity of NK/lymphokine-activated killer cells, activated T and NK cells and prompt IFN-gamma production (through resting PBMC). (prospecbio.com)
They are INTERLEUKIN-2-activated NK cells that have no MAJOR HISTOCOMPATIBILITY COMPLEX restriction or need for antigen stimulation. (nih.gov)
Lymphokine-activated killer (LAK) cells are produced from the patient's T cells which are extracted from the tumor and grown in a cell culture system with the lymphokine interleukin-2 (IL-2). (msdmanuals.com)
His studies deal with areas such as Interleukin 12 and Antigen-presenting cell as well as Interleukin 21. (research.com)
Interleukin-15-activation of natural killer cells overcame this reduced sensitivity. (biomedcentral.com)

If 2-mercaptoethanol was added to these culture media they were also capable of generating murine LAK cells which were effective in reducing pulmonary metastases in the murine MCA-106 model. (johnshopkins.edu)
15. Endogenous and adoptively transferred A-NK and T-LAK cells continuously accumulate within murine metastases up to 48 h after inoculation. (nih.gov)
19. Cellular basis of immunologic interactions in adoptive T cell therapy of established metastases from a syngeneic murine sarcoma. (nih.gov)
This reparative inflammatory response promotes tumor cell survival, expansion, and metastases. (elsevierpure.com)

The very few trials with lymphokine-activated killer (LAK)/cytokine-induced killer (CIK) cells tested only in Asian population have resulted in some clinical effects with prolonged survival. (medscape.com)
It's a powerful cytokine , a molecule that stimulates other cells to grow and mature. (medicallessons.net)
DC defects in maturation, cytokine production and antigen presentation 61, 62, 63 and T-cell defects in proliferation 64, 65, 66 have been reported in animal models and humans with chronic infections. (nika-robot.de)
it induces the proliferation of naive T cells selectively, acts with IL-12 to promote IFN- production and is the ligand for TCCR (T-cell cytokine receptor, also known as. (nika-robot.de)

The ED 50 as determined by the dose-dependent stimulation of the proliferation of murine CTLL-2 cells is ≤ 0.4 ng/ml, corresponding to a specific activity of ≥ 2.5 x 10 6 units/mg. (peprotech.com)

1987 ), promotes antibody production and proliferation by b cells ( mingari et al. (nika-robot.de)

IL12b/IL12a is a crucial part to the process of cellular-immunity and activates the differentiation of Th1 cells originates from the precursor T helper cells. (prospecbio.com)
PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. (thermofisher.com)
The activated nave cells undergo proliferation and differentiation and yield different subtypes of helper T cells with different functions. (nika-robot.de)
Yet, it is incompletely understood if targeting of CD71 also affects the differentiation and functional polarization of primary human T cells. (nika-robot.de)

LAK cells are also capable of acting against cells that do not display the major histocompatibility complex, as has been shown by the ability to cause lysis in non-immunogenic, allogeneic and syngeneic tumors. (wikipedia.org)
In melanoma and gastric cancer cells, intercellular adhesion molecule 1 (ICAM-1) antibody can significantly inhibit in vitro LAK-induced lysis of cancer cells. (wikipedia.org)
Nanomolar granulysin promotes chemotaxis and increases production of chemokines by monocytic cells, while micromolar local concentrations are needed for lysis (7). (rndsystems.com)
Study results demonstrated an increase in cell lysis by IL-2-activated NK and LAK cells, and a reduction in endothelial cell injury by taurine. (ndnr.com)

LAK cells respond to these lymphokines, particularly IL-2, by lysing tumor cells that were already known to be resistant to NK cell activity. (wikipedia.org)
LAK cells are specific to tumor cells and do not display activity against normal cells. (wikipedia.org)
The 51 chromium release assay has been considered as the gold standard method for measuring NK activity, but it has numerous limitations, such as radioactivity, interlaboratory variability, and low sensitivity to apoptotic cell death [ 12 ]. (hindawi.com)
Calcein-AM, a non-fluorescent substance which is intracellularly converted to the green fluorescent calcein by esterase activity in viable cells, is initially used to stain target cells. (nih.gov)
9. Antileukemia activity of a natural killer cell line against human leukemias. (nih.gov)
13. In vivo antitumor activity of T cells redirected with chimeric antibody/T-cell receptor genes. (nih.gov)
NK cells have a constitutive cytotoxic activity and kill a wide spectrum of tumor cells while T cells recognize specific tumor antigens and need to be activated through their TCR to differentiate into cytotoxic T lymphocyte (CTL) killers or T helper cells. (lu.se)
We could show that continuous IL-2/IL-2R stimulation through i.p. injections of rIL-2 twice a day had multiple effects on SEA induced T cell activity. (lu.se)
Exogenous IL-2 increased T cell expansion and superinduced as well as prolonged CTL activity. (lu.se)
Enhanced induction of lymphokine-activated killer activity after lentinan administration in patients with gastric carcinoma. (gmushrooms.com)
1983. Pretreatment with minor histocompatibility complex antigens prevents the development of functional CTL helper cell activity. (stjohns.edu)
Studies have also found that subjects with healthy lifestyles (breakfast consumption is cited as one aspect of a "healthy lifestyle") have higher activity levels of natural killer (NK) cells10 and lymphokine-activated killer cells11 than those with unhealthier lifestyles. (asai.ie)
In regards to the possibility of taurine reducing the antitumor efficacy of IL-2, it was found that taurine did not alter activated NK-cell-mediated antitumor function, yet it significantly enhanced LAK cell activity (Finnegan et al. (ndnr.com)
Vitamin C regulates cellular and humoral immune function, increases macrophage activity, and supports lymphocyte and NK cell activity. (ndnr.com)
The activity is determined by the IFN-g ELISA in a using NK-92 human natural killer cells. (prospecbio.com)
Thus, release of HMGB1 from dying tumor cells treated with chemotherapy or cells with lymphokine activated killer cell activity is not dependent solely on the mode of cell death. (elsevierpure.com)
Prevention of superantigen induced down-regulation of T cell mediated cytotoxic activity by IL-2 in vivo. (lu.se)
Form of adoptive transfer where cells with antitumor activity are transferred to the tumor-bearing host in order to mediate tumor regression. (nih.gov)
Conclusion: Laughter may reduce stress and improve NK cell activity. (studybuff.com)
As low NK cell activity is linked to decreased disease resistance and increased morbidity in persons with cancer and HIV disease, laughter may be a useful cognitive-behavioral intervention. (studybuff.com)
The Th cells do not have cytotoxic or phagocytic activity. (nika-robot.de)
besides its potent t-cell growth factor activity, il-2 induces proliferation of natural killer (nk) cells and augments their cytolytic activity as well as that of lymphokine-activated killer cells ( siegel et al. (nika-robot.de)

Antigens are substances (usually proteins) on the surface of cells, viruses, fungi, or bacteria4. (asai.ie)
Preclinical animal studies have identified tumor-specific antigens and the direct killing of tumor cells using T cells specific for these antigens ( 2 , 3 ). (frontiersin.org)
Targeted T-cell therapy consisting of vaccination or the adoptive transfer of T-cells against defined tumor-associated antigens (TAA) is a reasonable extension of established treatment strategies. (biomedcentral.com)
Besides the so-called neoantigens, which are generated by somatic mutations in the neoplastic cells (e.g., p53) and are usually patient-specific, the targetable TAAs in EOC are usually self-antigens, which are non-mutated proteins aberrantly expressed by the tumor. (biomedcentral.com)
Tumor Antigens Many tumor cells produce antigens, which may be released in the bloodstream or remain on the cell surface. (msdmanuals.com)

A study has shown that ICAM1 in lung cancer cells increases LAK cell-mediated tumor cell death as a new anti-tumor mechanism. (wikipedia.org)
[ 8 ] The thick fibrotic stroma increases the distance between the vessels and the tumor cells and mechanically hampers the diffusion of the infused drugs, which cannot reach the cancer cells in therapeutic concentrations. (medscape.com)
These calculations require an extension of a distills at 58.Rc, alcohol has been proposed as a useful technique to start the conversa- the risk level (9 7 per substance per unit volume, which increases cd8 and nk cell lymphokine-activated killer (lak) cells interferon (ifn) tent as indomethacin.21 the lipophilic phase. (stonecottagegardens.com)

Subsequently, studies on tumor-induced immune suppressive mechanisms have identified multiple suppressor cell populations and inhibitory molecules, all of which exert critical regulatory roles in tumor generation and progression ( 6 - 8 ). (frontiersin.org)
Likewise, low expression of inhibitory human leukocyte antigen (HLA) class I molecules is an important prerequisite for successful natural killer cell triggering. (biomedcentral.com)

The CD45 isoforms play complex roles in T-cell and B-cell antigen receptor signal transduction. (bdbiosciences.com)
Recent clinical success with immune checkpoint inhibitors, chimeric antigen receptor T-cell therapy, and adoptive immune cellular therapies has generated excitement and new hopes for patients and investigators. (frontiersin.org)
Treatment is independent of receptor density, heterogeneity, or the hypoxic status of cells. (snmjournals.org)
Chimeric antigen receptor (CAR) T cell therapy, Oncolytic viruses, Immune-checkpoint inhibitors, and Dendritic cells (DC) vaccination were the most encouraging areas in glioma therapy [ 2 , 3 ]. (aging-us.com)
Using flow cytometry, ELISA and immunohistochemistry, expression of natural killer cell receptor ligands was assessed in chemotherapy-sensitive/-resistant Ewing sarcoma cell lines, plasma and tumours. (biomedcentral.com)
Despite comparable expression of natural killer cell receptor ligands, chemotherapy-resistant Ewing sarcoma exhibited reduced susceptibility to resting natural killer cells. (biomedcentral.com)

The third unfortunate characteristic of PC is the abundant stroma that shields the tumors cells and defines its chemoresistance. (medscape.com)
Renal cell cancer is one of the few tumors in which well-documented cases of spontaneous tumor regression in the absence of therapy exist, but this regression occurs rarely and may not lead to long-term survival. (cancer.gov)
however, the two cell types may occur together in some tumors. (cancer.gov)
Some investigators have found that granular cell tumors have a worse prognosis, but this finding is not universal. (cancer.gov)
More than 85% of 111 In remained in tumors, bound to cell cytoplasmic components of apparent molecular weights 250 and 6 kDa. (snmjournals.org)

The mechanism of LAK cells is distinctive from that of natural killer cells because they can lyse cells that NK cells cannot. (wikipedia.org)
Activated CD3- CD16+ natural killer cells express a subset of the lymphokine genes induced in activated alpha beta + and gamma delta + T cells. (ucdenver.edu)
What are natural killer cells? (studybuff.com)

11. Visualization of effective tumor targeting by CD8+ natural killer T cells redirected with bispecific antibody F(ab')(2)HER2xCD3. (nih.gov)
therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. (bdbiosciences.com)
Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. (bdbiosciences.com)
however, CD19 expression, detectable by the rat anti-mouse CD19 antibody (clone 1D3), is reported to be more restricted to the B-cell lineage. (bdbiosciences.com)
The rat anti-mouse CD45R antibody (clone RA3-6B2) has been reported to enhance isotype switching during in vitro B-cell responses and to inhibit in vivo B-cell responses. (bdbiosciences.com)
Human peripheral blood mononuclear cells were treated for 24 hr with 50 ng/ml PMA and 200 ng/ml Ca 2+ ionomycin then stained with Goat Anti-Human Granulysin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3138, filled histogram) or control antibody (Catalog # AB-108-C , open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107 ). (rndsystems.com)
All cells were co-stained with CD3e (145-2C11) Monoclonal Antibody, eFluor 450 (Product # 48-0031-82). (thermofisher.com)
A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. (thermofisher.com)
Whenever possible, we recommend adding CellBlox Blocking Buffer to antibody cocktails/master mixes prior to combining with cells. (thermofisher.com)

The aim of this cross-sectional study was to determine whether immune cell counts in the peripheral blood were altered among tank entry workers at three Air Force bases. (nih.gov)
implying not only tolerance by the peripheral T-cell repertoire, but also the risk of immunogenic toxicity (on-target/off-tumor toxicity) in the case of an effective T-cell response. (biomedcentral.com)
There are two tests that may be done: peripheral natural killer cell testing and uterine natural killer cell testing. (studybuff.com)
Peripheral NK cell testing is a blood test and seeks to measure the percentage and quantity of NK cells in the bloodstream. (studybuff.com)

1982. The induction of alloreactive responses in vivo are inhibited by pre-treatment with Mls-disparate cells. (stjohns.edu)

Emerging therapeutic strategies for enhancing sensitivity and countering resistance to programmed cell death protein 1 or programmed death-ligand 1 inhibitors in non-small cell lung cancer. (viictr.org)

His research in Natural killer cell intersects with topics in Tumor necrosis factor alpha, Liver function and Interferon gamma. (research.com)

Liver-resident NK cells confer adaptive immunity in skin-contact inflammation. (research.com)
What cells plays important role in antitumor immunity? (studybuff.com)

Lymphokines are a subset of cytokines that are produced by a type of immune cell known as a lymphocyte. (studybuff.com)

Granulysin (formerly NKG5 or Lymphokine LAG-2) is a member of the saposin-like protein (SAPLIP) family of membrane disrupting proteins (1). (rndsystems.com)
We have recently demonstrated that cytolysis of human melanoma cells by immune effectors (both NK and T cells) is associated with release of the nuclear chromatin protein, high mobility group box I (HMGB1). (elsevierpure.com)
Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. (assaygenie.com)
They are protein mediators typically produced by T cells to direct the immune system response by signaling between its cells. (studybuff.com)
MS5033 is a potent PROTAC-based AKT ( protein kinase B) degrader, with a DC 50 of 430 nM in PC3 cells. (medchemexpress.com)
Cdc7-IN-5 (compound I- B ) is a potent Cdc7 kinase inhibitor extracted from patent WO2019165473A1, compound I- B . Cdc7 is a serine-threonine protein kinase enzyme which is essential for the initiation of DNA replication in the cell cycle. (medchemexpress.com)
OTS964 hydrochloride is an orally active, high affinity and selective TOPK (T-lymphokine-activated killer cell-originated protein kinase ) inhibitor with an IC 50 of 28 nM. (medchemexpress.com)
Signal 1 is provided by a foreign peptide bound to an MHC protein on the surface of the presenting cell. (nika-robot.de)

The CD45 isoforms detected in the mouse are cell type-, maturation, and activation state-specific. (bdbiosciences.com)
Activation of CD8 + cytotoxic T cells has long been regarded as a major antitumor mechanism of the immune system. (studybuff.com)
The generation of T cell subtypes is dependent on cytokines present in the micro-environment following T cell activation. (nika-robot.de)

It enhances the recognition of GBM cells by the patient's immune system, activates a broad, effective, and persistent immune response, and eliminates tumor cells. (aging-us.com)
The LAK cells produced are then returned to the patient's bloodstream. (msdmanuals.com)
In cancer therapy, TILs are removed from a patient's tumor, grown in large numbers in a laboratory, and then given back to the patient to help the immune system kill the cancer cells. (studybuff.com)

LAK cells have shown potential as a cellular agent for cancer therapy and have been utilized therapeutically in association with IL-2 for the treatment of various cancers. (wikipedia.org)
Renal cell cancer, also called renal adenocarcinoma or hypernephroma, can often be cured if it is diagnosed and treated when still localized to the kidney and the immediately surrounding tissue. (cancer.gov)
Because most patients are diagnosed when the tumor is still relatively localized and amenable to surgical removal, approximately 75% of all patients with renal cell cancer survive for 5 years. (cancer.gov)
For more information, see Transitional Cell Cancer of the Renal Pelvis and Ureter Treatment . (cancer.gov)
The staging system for renal cell cancer is based on the degree of tumor spread beyond the kidney. (cancer.gov)
The American Joint Committee on Cancer (AJCC) has designated staging by TNM (tumor, node, metastasis) classification to define renal cell cancer. (cancer.gov)
Some of these findings were recapitulated in humans and used to develop cancer immunotherapies based on provoking tumor-antigen-specific T-cell responses ( 4 , 5 ). (frontiersin.org)
The FDA subsequently expanded the approved use of nivolumab to patients with advanced squamous non-small cell lung cancer (NSCLC) with progression on or after platinum-based chemotherapy in 2015 ( 12 ). (frontiersin.org)
It is worth mentioning here that estrogen interacts with gene p53, an anti-cancer gene which is basically a 'molecular patrolman' that prevents all flawed cells from reproducing. (herbs2000.com)
However, gene p53 is not the only gene that controls the cancer cells. (herbs2000.com)
These tablets prevent the proliferation of cancer cells activated by estrogen, thereby harmonizing the use of tamoxifen (Nolvadex). (herbs2000.com)
Animal studies have shown that LAK cells are more effective against cancer cells than are the original endogenous T cells, presumably because of their greater number. (msdmanuals.com)
Therefore CAR T cells and TCR T cells may represent complementary approaches to cancer therapy. (msdmanuals.com)
Molife C, Brnabic A, Stefaniak VJ, Belger MA, Gruver K, Chen JV, Souri S, Blumenschein GR . Sintilimab plus chemotherapy for first-line treatment of advanced or metastatic nonsquamous non-small-cell lung cancer: network meta-analysis. (viictr.org)
TILs can recognize and kill cancer cells. (studybuff.com)

Individual fractions were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and tested for the ability to stimulate Babesia-specific CD4+ T-cell lines and clones. (nika-robot.de)

IL-2 is a powerful immunoregulatory lymphokine produced by T-cells in response to antigenic or mitogenic stimulation. (peprotech.com)

Dendritic cells are the most robust antigen-presenting cells. (aging-us.com)
Inhibition of allogeneic T cell proliferation by indoleamine 2,3-dioxygenase-expressing dendritic cells: mediation of suppression by tryptophan metabolites. (nika-robot.de)

IL-15 is necessary and sufficient for granulysin upregulation in CD8 T cells (2). (rndsystems.com)

The expression of some TAAs is irrelevant for the maintenance of the malignant phenotype, with unstable expression in the malignant cells (e.g. (biomedcentral.com)

It went by other names, too, including Helper Peak, T-Cell Replacing Factor III, and B-Cell Activating Factor (BAF). (medicallessons.net)
2.Helper T cells are activated 3.B cells differentiate into plasma cells and memory cells. (nika-robot.de)
Helper T-cells are a type of immune cell. (nika-robot.de)
These cells are subdivisions of the T helper cells. (nika-robot.de)
Taken together, these results suggest a role for SOCS3 in maintaining T-helper cells in a quiescent state. (nika-robot.de)

Evidence has shown DC-based vaccination could target and kill GBM cells efficiently with little damage to the surrounding healthy tissues [ 4 - 8 ]. (aging-us.com)

LAK cells have anticancer efficacy against homologous carcinoma cells and can grow ex vivo in the presence of IL-2. (wikipedia.org)

Dendritic cell (DC) vaccines have been associated with some clinical objective response and prolonged survival in few patients with delayed type hypersensitivity reactions. (medscape.com)

However, some activated T cells, lymphokine activated killer cells (LAK), NK cell progenitors in the bone marrow, and T cells of the lpr/lpr mutant mouse also express this antigen. (thermofisher.com)
NK cells develop in bone marrow as well as in some extramedullar sites, such as lymph nodes, thymus, liver, and uterus. (studybuff.com)

IL-2/IL-2R signaling is required for T-cell proliferation and other fundamental functions that are essential for the immune response. (peprotech.com)
T lymphocyte proliferation and lethal effect of cytotoxic T cell (CTL) were detected by CCK-8. (aging-us.com)

Although LAK cells generated in these media have not been tested in humans yet, potentially they could provide a safe, unlimited and less expensive source of culture fluid for generating the large numbers of LAK cells needed for human clinical trials. (johnshopkins.edu)
Clinical trials of LAK cells in humans are ongoing but this approach has not gained widespread use and is generally considered less effective than other cell therapies. (msdmanuals.com)

The effects of jet fuel on immune cells of fuel system maintenance workers. (nih.gov)
12 ) For years it had been proved that immune cells use glucose as a fuel ( 13 ), but in the early '80s, it was found that these cells use GLN equally and in the same way as glucose ( 14 ). (freedissertation.com)
Lymphokines act to attract additional immune cells to mount an immune response, for instance in stimulating B cells to generate antibodies against the invading pathogen. (studybuff.com)

Investigations are needed to evaluate the functional ability of these cells to produce lymphokines and cytokines and modulate the immune system. (nih.gov)

Anatomy43

Organisms13

Diseases15

Chemicals and Drugs122

Analytical, Diagnostic and Therapeutic Techniques and Equipment25

Phenomena and Processes40

Technology, Industry, Agriculture1

Information Science3

Autologous6

Lymphocytes21

Immunotherapy17

Lymphoid cells4

Recombinant2

Cytotoxic cells4

Effector12

Receptors5

Monocytes1

Cytotoxicity16

Innate4

Immunology8

Interleukin12

Metastases4

Cytokine4

Murine1

19871

Differentiation4

Lysis4

Activity22

Antigens5

Increases3

Inhibitory2

Receptor6

Tumors5

Natural kille3

Antibody9

Peripheral4

Induction1

Inhibitors1

Interferon1

Immunity2

Subset1

Protein8

Activation3

Patient's3

Cancer15

Stimulate1

Immunoregulatory1

Dendritic cells2

Upregulation1

Malignant cells1

Helper5

Vaccination1

Vivo1

Vaccines1

Bone marrow2

Proliferation2

Humans2

Immune cells3

Cytokines1