Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human KCNAB1. (prosci-inc.com)
Peptide2
Synthetic peptide within Human SRGAP1 aa 1050 to the C-terminus (C terminal). (abcam.co.jp)
Synthetic peptides or recombinant fragments containing bio- logically active motifs have been shown to partially mimic the effects of native laminin in various cellular functions, such as cell adhesion, spreading, migration, neurite outgrowth, and tumor metastasis [6-10], suggesting that laminin-derived-short peptide- modified biomaterials may have therapeutic potential in tissue regeneration applications. (mirnamimic.com)
Cell4
However, in order to use this method to investigate SH2 binding sites on a specific target in cell lysate, additional procedures such as pull-down or immunoprecipitation which consume large amounts of sample are required. (biomedcentral.com)
We showed for the first time that interactions between SH2 domain probes and EGFR in cell lysate can be determined in a microliter-scale assay using SH2-PLA. (biomedcentral.com)
The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
In experiments probing for a role of PKCa in human laminin-2-integrin-mediated cell adhesion and spreading of PC12 cells, we observed unexpected enhancements of adhesion, spreading and stress fiber formation to 1 mM Go¨6976 with concomitant increase in membrane translocation of PKCd and autophosphorylation of focal adhesion kinase (FAK). (mirnamimic.com)
Kinase2
There is a great interest in studying phosphotyrosine dependent protein-protein interactions in tyrosine kinase pathways that play a critical role in many aspects of cellular function. (biomedcentral.com)
Recent reports demonstrate that the expression of protein kinase C alpha (PKCα) in triple-negative breast cancer (TNBC) correlates with decreased survival outcomes. (oncotarget.com)
Lung1
Further, we showed that PLA-SH2 can survey lung cancer tissues using 1 μl lysate without requiring phospho-enrichment. (biomedcentral.com)
Interacts2
In our recent study, it was revealed that PXR interacts with DAZ-associated protein 1 (DAZAP1), which is an essential component of the paraspeckle, a membraneless nuclear body, and the interaction was disassociated by rifampicin, a ligand of PXR. (aspetjournals.org)
In this study, we show that contactin associates with Na v 1.3 in P0 brain tissue and interacts with both the N and C termini of Na v 1.3. (jneurosci.org)
Mice2
We found that the MCEPH protein is expressed in the brain of mceph/mceph mice. (biomedcentral.com)
Quantification of renal cysts Kidneys were eliminated from control mice at 12?weeks of age, fixed in 10% neutral buffered formalin and bisected before handling and paraffin embedding. (immune-source.com)
Nuclear3
Human pregnane X receptor (PXR) is a major nuclear receptor that upregulates the expression of drug-metabolizing enzymes such as CYP3A4. (aspetjournals.org)
This protein is thought to be involved inattaching the base of chromatin loops to the nuclear matrix but there is conflicting evidence as towhether this protein is a component of chromatin or a nuclear matrix protein. (immune-source.com)
Scaffoldattachment factors are a specific subset of nuclear matrix proteins (NMP) that specifically bind toS/MAR. The encoded protein is thought to serve as a molecular base to assemble atranscriptosome complex in the vicinity of actively transcribed genes. (immune-source.com)
In this study we show that contactin coimmunoprecipitates with Na v 1.3 from postnatal day 0 rat brain where this channel is abundant, and from human embryonic kidney (HEK) 293 cells stably transfected with Na v 1.3 (HEK-Na v 1.3). (jneurosci.org)
Renal1
Experimental evidence suggests that these factors may be causally related to CVD, but the direction of causality is difficult to determine, as a major confounder is their reduced renal clearance or metabolism with reduced kidney function. (biomedcentral.com)
Interaction1
The obvious benefit of this method is that the low sample requirement allows detection of SH2 binding in samples which are difficult to analyze using traditional protein interaction assays. (biomedcentral.com)
Mouse3
IHC-P: Human tonsil, Human bladder cancer, mouse stomach and rat cerebrum tissues. (abcam.com)
By using Fh1-deficient mouse epithelial kidney cells we have recently demonstrated that in the absence of Fh1, the TCA cycle is definitely truncated causing an build up of fumarate and succinate paralleled by a decrease of malate and citrate. (immune-source.com)
Ultrasound Large resolution ultrasound imaging of mouse kidneys was performed as previously explained [17], using a Vevo 770 system (Visual Sonics, Toronto, Canada) with a 25?MHz transducer and 6?mm depth scanhead. (immune-source.com)
Gene1
This gene encodes a DNA-binding protein which has high specificity for scaffold or matrixattachment region DNA elements (S/MAR DNA). (immune-source.com)
Specific1
SPECIFIC INFECTIONS Necrotizing soft tissue infections Diagnosis and management of primary, secondary, and tertiary peritonitis High-risk patients: Who are they and how should they be managed? (docksci.com)
Expression1
This study shows that the expression of both myeloid zinc finger 1 (MZF-1) and Ets-like protein-1 (Elk-1) correlates with PKCα expression in TNBC. (oncotarget.com)
Heart1
Macrophages are known to be involved in both chronic kidney disease (CKD) and heart failure, however their role in the development of cardiorenal syndrome is less clear. (biomedcentral.com)
Sequence1
In PC12 cells, we recently showed that the engagement of integrin a3b1 to a binding sequence within the human laminin a2 chain was sufficient to recruit PKCa and PKCd to the integrin receptor [6]. (mirnamimic.com)
Domain1
The predicted truncated Kv1.1 protein (MCEPH) will retain only the N-terminal (T1) domain, the first transmembrane domain (S1) and the first extracellular loop. (biomedcentral.com)
Previously1
Previously, we have reported neurotrophism exhibited by human amniotic epithelial cells when transplanted after spinal cord injury in bonnet monkeys. (scielo.org.ar)
Function1
We found that reduced kidney function resulted in the expansion of cardiac macrophages, in particular through local proliferation of resident populations. (biomedcentral.com)
Diverse1
The relationship between monocyte subset and macrophage phenotype is still to be resolved, however tissue macrophages clearly have diverse functions and are key inflammatory mediators [ 23 ]. (biomedcentral.com)
Found1
In humans, we found increased plasma CXCL10 concentrations in advanced CKD, and identified the production of CXCL10 in cardiomyocytes and cardiac fibroblasts. (biomedcentral.com)
Important2
Macrophages have been shown to be important in all stages of inflammatory and fibrotic kidney disease [ 11 ]. (biomedcentral.com)
An important consequence of reversible phosphorylation of tyrosine residues on proteins is the creation of binding sites for phosphotyrosine recognizing domains such as Src homology 2 (SH2) and phosphotyrosine binding (PTB) domains [ 1 , 2 ]. (biomedcentral.com)
Primary1
We quantified CXCL10 in human CKD plasma and tested the response of human iPSC-derived cardiomyocytes and primary cardiac fibroblasts to serum from CKD donors. (biomedcentral.com)