• We identify the main binding sites of a catalytically inactive Cas9 (dCas9) protein in bovine fetal fibroblast cells (BFFs) with chromatin immunoprecipitation sequencing (ChIP-seq). (biomedcentral.com)
  • IP: HeLa whole cell lysate. (abcam.com)
  • Western blot detection of human Polycystin-1L2 in HeLa cell lysate. (biosensis.com)
  • Recent reports demonstrate that the expression of protein kinase C alpha (PKCα) in triple-negative breast cancer (TNBC) correlates with decreased survival outcomes. (oncotarget.com)
  • This gene encodes a member of the vaccinia-related kinase (VRK) family of serine/threonine protein kinases. (thermofisher.com)
  • The PTK2 gene encodes a cytosolic protein tyrosine kinase. (faksignaling.com)
  • Product Description google Mouse anti-Polycystic kidney disease protein 1-like 2 Monoclonal Antibody (Unconjugated), suitable for WB, ELISA. (biosensis.com)
  • Western blot was performed using Anti-SOD3 Polyclonal Antibody (Product # PA5-80052) and a 30 kDa band corresponding to Extracellular superoxide dismutase [Cu-Zn] was observed across tissues tested except Skeletal muscle which is reported to be low to negative for SOD3 expression. (thermofisher.com)
  • Western Blot analysis of Mouse liver, Mouse kidney and Human fetal liver using KHK Polyclonal Antibody at dilution of 1:1350. (elabscience.com)
  • Various lysates were subjected to SDS PAGE followed by western blot with 15004-1-AP (TCTN1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. (ptglab.com)
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15004-1-AP (TCTN1 Antibody) at dilution of 1:50 (under 40x lens). (ptglab.com)
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15004-1-AP (TCTN1 Antibody) at dilution of 1:50 (under 10x lens). (ptglab.com)
  • Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using TCTN1 antibody (15004-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). (ptglab.com)
  • Ras signaling can be thought of in simple terms as occuring downstream of receptor tyrosine kinases, triggered by association of adaptor proteins with the phosphorylated cytoplasmic tails of these receptors. (sdbonline.org)
  • It is a multi-pass transmembrane protein that is expressed in all tissues tested including brain, placenta, mammary gland, testis, lung and liver. (biosensis.com)
  • Tissue extracts (60 µg lysate) of Mouse Lung (Lane 1), Mouse Skeletal Muscle (Lane 2), and Mouse Aorta (Lane 3) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). (thermofisher.com)
  • Reverse transcription-polymerase chain reaction of total lung RNA showed low levels of UGT1A10 expression in human lung tissue. (aspetjournals.org)
  • Alveolar macrophages (AMs) are the tissue-resident macrophages in the lung alveolar space. (frontiersin.org)
  • The authors recommend focusing on the clinical history, the placental evaluation, the external examination of the fetus, and, when available, fetal tissue obtained by MITS, especially of the lung (focused on histology and microbiology) and brain/cerebral spinal fluid (CSF) and fetal blood (focused on microbiological analysis). (cdc.gov)
  • However, these chemotherapeutic agents can cause a range of dose-limiting toxicity (DLT) symptoms, such as weight loss and heart, liver, and kidney toxicity [ 4 ]. (hindawi.com)
  • This gene is widely expressed in human tissues and has increased expression in actively dividing cells, such as those in testis, thymus, fetal liver, and carcinomas. (thermofisher.com)
  • Antithyroid drugs taken by the mother cross the placenta, treating potential fetal hyperthyroidism, but they may also cause transient fetal and neonatal hypothyroidism. (bvsalud.org)
  • Synthetic peptides or recombinant fragments containing bio- logically active motifs have been shown to partially mimic the effects of native laminin in various cellular functions, such as cell adhesion, spreading, migration, neurite outgrowth, and tumor metastasis [6-10], suggesting that laminin-derived-short peptide- modified biomaterials may have therapeutic potential in tissue regeneration applications. (mirnamimic.com)
  • RON is an RTK that mediates the biological effects of mac-rophage-stimulating protein (MSP) (20, 21). (123dok.org)
  • The spike protein of coronaviruses mediates virus binding and cell entry. (cdc.gov)
  • In fact, it is known that the development of cell immunity in pulmonary tissues is largely independent of the events occurring in both peripheral circulation and distal lymphoid organs. (nature.com)
  • and Compact disc8 T cell immunity to these protein, while VRPs expressing gp350 didn't induce detectable T cell immunity to MK-8245 gp350. (exatecan-mesylate.com)
  • Our goals were twofold: 1) to characterize the gene expression, protein expression, and activity of key synthesizing and regulating enzymes of energy metabolism in the whole mouse retina, retinal compartments, and/or cells and 2) to provide an integrative analysis of the results related to function. (molvis.org)
  • It is recommended to add 0.1 mL of RIPA Lysis Buffer to each well of a 6-well plates (the protein content in different cells may vary, and the volume of the lysate added can be appropriately adjusted). (elabscience.com)
  • Conclusion: EC and DHBA regulate the renal glucose homeostasis by modulating both glucose uptake and production, and strengthen the insulin signalling by activating key proteins of that pathway in NRK-52E cells. (faksignaling.com)
  • By using Fh1-deficient mouse epithelial kidney cells we have recently demonstrated that in the absence of Fh1, the TCA cycle is definitely truncated causing an build up of fumarate and succinate paralleled by a decrease of malate and citrate. (immune-source.com)
  • The results demonstrate that DLEU1 and TRAF4 is highly expressed in GBM tissues and promotes proliferation of GBM cells. (spandidos-publications.com)
  • Cell culture or tissue culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. (wikipedia.org)
  • After the cells of interest have been isolated from living tissue, they can subsequently be maintained under carefully controlled conditions. (wikipedia.org)
  • Tissue culture commonly refers to the culture of animal cells and tissues, with the more specific term plant tissue culture being used for plants. (wikipedia.org)
  • In practice, the term "cell culture" now refers to the culturing of cells derived from multicellular eukaryotes, especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture, fungal culture, and microbiological culture (of microbes). (wikipedia.org)
  • The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century. (wikipedia.org)
  • He suggested that the potentialities of individual cells via tissue culture as well as that the reciprocal influences of tissues on one another could be determined by this method. (wikipedia.org)
  • In modern usage, "tissue culture" generally refers to the growth of cells from a tissue from a multicellular organism in vitro. (wikipedia.org)
  • LCN2 protein induced upregulation of glial fibrillary acidic protein (GFAP), cell migration, and morphological changes similar to characteristic phenotypic changes termed reactive astrocytosis. (jneurosci.org)
  • The predicted MCEPH protein is truncated at amino acid 230 out of 495. (biomedcentral.com)
  • In this process, astrocytes proliferate to fill gaps and undergo a typical morphological change: a large cytoplasmic mass, long and branched processes, and increased expression of intermediate filaments such as glial fibrillary acidic protein (GFAP). (jneurosci.org)
  • The ECM proteins bind to the extracellular domains of integrin heterodimers, whereas the actin stress fibers link to integrin cytoplasmic tails via large molecular complexes. (rupress.org)
  • Tensin is a gene family with four members (tensin1, tensin2, tensin3, and cten), and their encoding proteins are localized to the cytoplasmic side of focal adhesions. (rupress.org)
  • The public health community requires viral lysates to serve as diagnostic references, and the research community needs virus isolates to test antiviral compounds, develop new vaccines, and perform basic research. (cdc.gov)
  • This gene encodes a DNA-binding protein which has high specificity for scaffold or matrixattachment region DNA elements (S/MAR DNA). (immune-source.com)
  • The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
  • Methods and results: Levels of the glucose transporters SGLT-2 and GLUT-2, as well as glucose uptake, glucose production, and key proteins of the insulin pathways, namely insulin receptor (IR), insulin receptor substrate-1 (IRS-1), and PI3K/AKT pathway were analysed. (faksignaling.com)
  • Taken together, our study demonstrated that GO-AgNPs potentially induce oxidative damage to DNA, which result in toxicity and cell apoptosis in caprine fetal fibroblast cell due to an increased generation of ROS. (researchsquare.com)
  • The predicted truncated Kv1.1 protein (MCEPH) will retain only the N-terminal (T1) domain, the first transmembrane domain (S1) and the first extracellular loop. (biomedcentral.com)
  • We recently provided evidence that the generation of endogenous extracellular vesicles (EVs) engineered for the incorporation of Severe Acute Respiratory Syndrome Coronavirus (SARS-CoV)-2 Nucleocapsid (N) protein induced immunity in the lungs of K18-hACE2 transgenic mice, which then can survive the lethal virus infection. (nature.com)
  • Detection of Cre-mediated recombination Ten m cryosections were slice from click freezing kidneys and -galactosidase activity was scored using X-gal substrate following a standard process (IHC World, Woodstock, MD, USA). (immune-source.com)
  • We found that the MCEPH protein is expressed in the brain of mceph/mceph mice. (biomedcentral.com)
  • In vivo , OBEX reduces weight gain by decreasing adiposity gain and increasing energy expenditure in high fat diet-fed mice through the activation of thermogenesis in brown adipose tissue (BAT) independent of eating behaviors. (nature.com)
  • Quantification of renal cysts Kidneys were eliminated from control mice at 12?weeks of age, fixed in 10% neutral buffered formalin and bisected before handling and paraffin embedding. (immune-source.com)
  • Macrophages are known to be involved in both chronic kidney disease (CKD) and heart failure, however their role in the development of cardiorenal syndrome is less clear. (biomedcentral.com)
  • We found that reduced kidney function resulted in the expansion of cardiac macrophages, in particular through local proliferation of resident populations. (biomedcentral.com)
  • Macrophages have been shown to be important in all stages of inflammatory and fibrotic kidney disease [ 11 ]. (biomedcentral.com)
  • The relationship between monocyte subset and macrophage phenotype is still to be resolved, however tissue macrophages clearly have diverse functions and are key inflammatory mediators [ 23 ]. (biomedcentral.com)
  • Mouse and rat brain and heart lysates. (abcam.com)
  • In response to a brain injury, astrocytes proliferate and become hypertrophic with an increased expression of intermediate filament proteins. (jneurosci.org)
  • Housekeeping genes in the FSCN1 - ACTB (F-A) locus (Fig. 1a ) have relatively steady expression levels across various tissues and thus exogenous gene silencing resulting from chromatin inactivation might be avoided. (biomedcentral.com)
  • Scaffoldattachment factors are a specific subset of nuclear matrix proteins (NMP) that specifically bind toS/MAR. The encoded protein is thought to serve as a molecular base to assemble atranscriptosome complex in the vicinity of actively transcribed genes. (immune-source.com)
  • This review focuses on the diagnosis and management of these patients during the fetal and neonatal periods. (bvsalud.org)
  • Six 4?m sections (taken at a 100?m time periods) were slice through each kidney and stained with hematoxylin and eosin. (immune-source.com)
  • During development it is expressed in fetal heart. (biosensis.com)
  • This review summarizes evidence for the role of proteolytic enzymes that degrade and inactivate insulin-like growth factor binding proteins (IGFBP) during follicular development in mammals. (bioone.org)
  • Geranylgeranyl transferase-I (GGTase-I) is one of the key enzymes mediating protein isoprenylation during the posttranslational modification, especially the geranylgeranylation of small GTPase, Rac1 [ 6 ]. (hindawi.com)
  • It conjugates to drugs to make them more soluble for excretion, is a cofactor for some enzymes, is involved in protein disulfide bond rearrangement and reduces peroxides. (lookformedical.com)
  • This protein is thought to be involved inattaching the base of chromatin loops to the nuclear matrix but there is conflicting evidence as towhether this protein is a component of chromatin or a nuclear matrix protein. (immune-source.com)
  • Experimental evidence suggests that these factors may be causally related to CVD, but the direction of causality is difficult to determine, as a major confounder is their reduced renal clearance or metabolism with reduced kidney function. (biomedcentral.com)
  • This protease activity has recently been ascribed to serine metalloprotease(s), including pregnancy-associated plasma protein-A (PAPP-A), which was first detected in human follicular fluid nearly 20 yr ago. (bioone.org)
  • IHC-P: Human tonsil, Human bladder cancer, mouse stomach and rat cerebrum tissues. (abcam.com)
  • Ultrasound Large resolution ultrasound imaging of mouse kidneys was performed as previously explained [17], using a Vevo 770 system (Visual Sonics, Toronto, Canada) with a 25?MHz transducer and 6?mm depth scanhead. (immune-source.com)
  • The leading cause of death in end-stage kidney disease is related to cardiovascular disease. (biomedcentral.com)
  • Even mild chronic kidney disease (CKD) with isolated albuminuria has been associated with a 2- to 4-fold increased risk of cardiovascular events [ 1 ]. (biomedcentral.com)
  • As the disease progresses, joints, muscles, and subcutaneous tissues may become the sites of hemorrhage. (lookformedical.com)
  • Subsequently, we demonstrate that a single Cas9n-induced single-strand break can stimulate the insertion of the natural resistance-associated macrophage protein-1 ( NRAMP1 ) gene with reduced, but still considerable, off-target effects. (biomedcentral.com)
  • In addition, animals that received the rhesus EBV glycoprotein and became infected had a lower level of rhesus EBV DNA in the blood at 23 months after challenge than animals that received the rhesus EBV latency protein vaccine that subsequently were infected. (exatecan-mesylate.com)
  • Polycystin-1L2 may function as an ion-channel regulator and as a G-protein-coupled receptor. (biosensis.com)
  • It is involved in theregulation of heat shock protein 27 transcription, can act as an estrogen receptor co-repressorand is a candidate for breast tumorigenesis. (immune-source.com)
  • Its protein localizes to the nucleus and has been shown to promote the stability and nuclear accumulation of a transcriptionally active p53 molecule and, in vitro, to phosphorylate Thr18 of p53 and reduce p53 ubiquitination. (thermofisher.com)
  • This study shows that the expression of both myeloid zinc finger 1 (MZF-1) and Ets-like protein-1 (Elk-1) correlates with PKCα expression in TNBC. (oncotarget.com)
  • Forced expression of lcn2 or treatment with LCN2 protein increased the sensitivity of astrocytes to cytotoxic stimuli. (jneurosci.org)
  • Among them, rhodomycin A was the most efficient at reducing the activity and expression of Src in a dose-dependent manner, which was also the case for Src-associated proteins, including EGFR, STAT3, and FAK. (oncotarget.com)
  • RT‑PCR demonstrated that the expression levels of DLEU1 and TRAF4 were increased in GBM tissues. (spandidos-publications.com)
  • For example, lncRNA nuclear paraspeckle assembly transcript 1 (NEAT1) is highly expressed in GBM tissues and perturbed expression of NEAT1 suppresses cell proliferation and invasion ( 10 ). (spandidos-publications.com)
  • SPECIFIC INFECTIONS Necrotizing soft tissue infections Diagnosis and management of primary, secondary, and tertiary peritonitis High-risk patients: Who are they and how should they be managed? (docksci.com)
  • In view of the quite low mutation rate of the N protein, the present vaccine strategy has the potential to control the replication of all emerging variants. (nature.com)
  • All vaccine preparations were conceived to elicit anti-Spike protein immune responses, and their effectiveness relies on the generation of neutralizing antibodies. (nature.com)
  • We developed a CD8 + T-cell-based vaccine platform based on intramuscular (i.m.) injection of a DNA vector coding for antigens of interest fused at the C-terminus of a biologically inactive Human Immunodeficiency Virus (HIV)-Type 1 Nef protein (Nef mut ) having an unusually high efficiency of incorporation into EVs. (nature.com)
  • This vaccine was made possible by the cell culture research of John Franklin Enders, Thomas Huckle Weller, and Frederick Chapman Robbins, who were awarded a Nobel Prize for their discovery of a method of growing the virus in monkey kidney cell cultures. (wikipedia.org)
  • Take the supernatant and measure the protein concentration mentioned in step2. (elabscience.com)
  • Adjust the protein concentration with PBS Buffer. (elabscience.com)
  • The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture. (wikipedia.org)
  • Recently, new tools for LMIC settings to determine cause of death in stillbirths, including minimally invasive tissue sampling (MITS) - a method using needle biopsies to obtain internal organ tissue from deceased fetuses for histology and pathogen identification in those tissues have become available. (cdc.gov)
  • Weigh and smash the tissue, add an appropriate ratio of RIPA Lysis Buffer ( Cat# E-BC-R327 )(add 10 μL PMSF and 10 μL Na 3 VO 4 to each 1 mL RIPA Lysis) and homogenizely lyse the tissue. (elabscience.com)
  • For example, add 1 mL RIPA Lysis Buffer to 0.3 g tissue sample, the specific volume can be adjusted according to experimental requirements. (elabscience.com)
  • Add an appropriate ratio of RIPA Lysate Buffer (10 μL PMSF and 10 μL Na 3 VO 4 in each 1 mL RIPA Lysis) and lyse on the ice for 30 min. (elabscience.com)
  • Add 5 × SDS Loading Buffer ( Cat# E-BC-R288 ) with the ratio of the protein sample: 5 × SDS Loading Buffer = 4:1 and boil the mixture for 10 min. (elabscience.com)
  • In 1996, the first use of regenerative tissue was used to replace a small length of urethra, which led to the understanding that the technique of obtaining samples of tissue, growing it outside the body without a scaffold, and reapplying it, can be used for only small distances of less than 1 cm. (wikipedia.org)
  • They counteract the harmful and damaging effects of oxidation in animal tissues. (lookformedical.com)
  • Take the samples, wash the tissue thoroughly with pre-cooled PBS (0.01 M, pH=7.4)( Cat# E-BC-R187 ) to remove the surface blood and internal debris. (elabscience.com)
  • While gp350 is certainly important for security from infectious mononucleosis, EBV protein portrayed during latency. (exatecan-mesylate.com)