• Three cell lines were used as in vitro model of urolithiasis: MDCK I, MDCK II and LLC-PK1. (kbco.hr)
  • Representative compounds from each class of drugs were further tested as Pgp substrates, defined by the ability of human Pgp or mouse mdr1a/Pgp to transport them across a polarized kidney epithelial cell in vitro. (nih.gov)
  • We are taking comprehensive approaches and applying multiple model systems, including in vitro 3D culture, in vivo live image technology, in vitro organ culture, in vivo live image technology, in vitro organ culture/tissue slice culture, isolated kidney perfusion, mouse models and zebrafish models to study protein trafficking/epithelial polarity/remodeling in various kidney disease contexts. (massgeneral.org)
  • Next, using functional adhesion assay, we demonstrate that CTS-induced increases in the adhesion of macrophages to renal epithelial cells were significantly diminished after reduction of NKA α-1 in either macrophages or renal epithelial cells as well after inhibition of NKA α-1-Src signaling cascade with a specific peptide inhibitor, pNaKtide in vitro. (mcw.edu)
  • Finally, CTS-induced expression of adhesion markers in both endothelial and immune cells was significantly inhibited in an NKA α-1-Src signaling dependent manner in vitro. (mcw.edu)
  • In addition, we studied the stability of both isomers in aqueous solution and compared their antiproliferative activity in vitro in three human cancer cell lines CH1 (ovarian carcinoma), SW480 (colon carcinoma) and A549 (non-small Dimethyl sulfoxide cell lung cancer) and in vivo in a Hep3B SCID mouse xeno-transplantation model in order to establish whether tautomer identity in 1 and 2 has any effect on biological properties. (erksignal.com)
  • However, advances in cell and tissue culture will permit the development of in vitro toxicity assays. (pharmacy180.com)
  • The aim of the development of in vitro tests is not only to replace in vivo animal testing but also to study the mechanisms of cell modulation by toxic compounds. (pharmacy180.com)
  • Recently, for example, in vitro studies involving renal cells in culture suggested that the underlying mechanism of the proteinuria associated with the use of rosuvastatin was inhibition by the statin of the endocytotic uptake of proteins by the proximal tubular cell (Verhulst, D'Haese and De Broe, 2004). (pharmacy180.com)
  • The goal of this study was to investigate constitutive differences in the susceptibility of renal cortex cells originating from human, rat and porcine origin in vitro. (uni-konstanz.de)
  • Deore SS, Zalte AG, Gulecha VS. MTT Cytotoxicity Study of Extracts of Meyna laxiflora Seeds and Tectona grandis Bark using Cell Lines LLC PK1. (clinicsinoncology.com)
  • AA cytotoxicity was observed in all cell types in a time- and concentration dependent manner with species-specific differences, with porcine cells being the most sensitive. (uni-konstanz.de)
  • Rhamnaceae) on cisplatin-induced damage to kidney epithelial LLC-PK1 cells via mitogen-activated protein kinase (MAPK) and apoptosis pathways. (mdpi.com)
  • Studies in various tissues, including the kidney, have demonstrated that toxins elicit apoptosis under certain conditions and necrosis under others. (elsevierpure.com)
  • To investigate the protective effect of Edaravone (EDa) against apoptosis induced by Gentamicin (GM) in MDCK cells, MDCK cells were treated with 4mmol/L GM for 24h following pre-incubation with or without EDa (40μmol/L) for 30 min. (biomedres.us)
  • The results showed that EDa significantly inhibited GM-induced apoptosis in MDCK cells. (biomedres.us)
  • Studies have shown that GM can cause damage and apoptosis of rat tubule cells through oxidative stress [3-5]. (biomedres.us)
  • However, whether it inhibits the apoptosis of MDCK cells through antioxidation has not been widely studued. (biomedres.us)
  • The expression of mitochondrial apoptosis pathway-associated proteins was detected by western blot to explore the protective effect of EDa in GM- induced MDCK cell apoptosis. (biomedres.us)
  • Accutase cell detachment solution and an Annexin V-fluorescein isothiocyanate/propidium iodide (FITC/PI) apoptosis detection kit was purchased from Becton Dickinson (San Diego, CA, USA). (biomedres.us)
  • Wu X, Song M, Qiu P, Li F, Wang M, Zheng J, Wang Q, Xu F, Xiao H. A metabolite of nobiletin, 4'-demethylnobiletin and atorvastatin synergistically inhibits human colon cancer cell growth by inducing G0/G1 cell cycle arrest and apoptosis. (umassmed.edu)
  • Induction of cell cycle arrest and apoptosis with downregulation of Hsp90 client proteins and histone modification by 4? (umassmed.edu)
  • It can reduce LLC-PK1 cells apoptosis caused by PDCoV infection. (biomedcentral.com)
  • Linear relationship of phlorizin-binding capacity and hexose uptake during differentiation in a clone of LLC-PK1 cells. (cellosaurus.org)
  • We are also interested in studying the mechanism of renal epithelial cell regeneration and differentiation during tubular repair/remodeling after tubular injury. (massgeneral.org)
  • Human colonic ADENOCARCINOMA cells that are able to express differentiation features characteristic of mature intestinal cells such as the GOBLET CELLS. (umassmed.edu)
  • Wu X, Zhang N, Lee MM. Mullerian inhibiting substance recruits ALK3 to regulate Leydig cell differentiation. (umassmed.edu)
  • Kidney stones are mostly composed of calcium oxalate, which predominantly generates free radicals that are toxic to renal tubular cells. (kbco.hr)
  • HG increased viable mesangial cells (ihMC) in 48 h, while epithelial tubular cells were not affected (LLC-PK1 and MDCK). (unifesp.br)
  • Under normal conditions, a minute amount of low molecular weight proteins are filtered, which then undergo endocytic reabsorption by prox-imal tubular cells. (pharmacy180.com)
  • For exam-ple, alanine aminopeptidase, alkaline phosphatase and glutamyltransferase are enzymes bound to the brush border of proximal tubular cells. (pharmacy180.com)
  • The most widely used renal epithelial cell lines of animal origin are the LLC-PK1 (Hampshire pig) and OK (American opossum) cell lines, exhibiting characteristics suggestive of proxi-mal tubular origin, and the MDCK (Cocker Spaniel) cell line, exhibiting characteristics suggestive of distal origin. (pharmacy180.com)
  • Oxytocin induces a transient increase in cytosolic free [Ca2+] in renal tubular epithelial cells: evidence for oxytocin receptors on LLC-PK1 cells. (aspetjournals.org)
  • We examined the effects of oxytocin on renal tubular epithelial LLC-PK1 cells. (aspetjournals.org)
  • In cells loaded with Fura 2, we found that 1 microM oxytocin induced a rapid increase in cytosolic free [Ca2+]i from 120 nM to 250 nM within 12 sec. (aspetjournals.org)
  • Using peptide analogs, a poor correlation was found between antagonism of oxytocin-induced calcium transients of LLC-PK1 cells and pig kidney V2 and rat liver V1 receptor affinity. (aspetjournals.org)
  • These data indicate that oxytocin-induced calcium transients in LLC-PK1 cells were not mediated by V1 or V2 vasopressin receptors, but by oxytocin receptors. (aspetjournals.org)
  • However, the poor correlation between antagonism at the LLC-PK1 receptors and the rat uterus oxytocin receptors suggests marked differences in antagonist recognition. (aspetjournals.org)
  • We conclude that in LLC-PK1 cells, oxytocin stimulates a transient rise in cytosolic free [Ca2+]i and the formation of inositol phosphates, including Ins1,4,5P3. (aspetjournals.org)
  • 2017) 'Antioxidant Pre-treatment Reduces the Toxic Effects of Oxalate on Renal Epithelial Cells in a Cell Culture Model of Urolithiasis', International Journal of Environmental Research and Public Health , 14(1), str. (kbco.hr)
  • Oxidative stress was induced by exposure of cells to sodium oxalate in concentration of 8 mM. (kbco.hr)
  • The present studies examined the response of renal epithelial cells to oxalate, a metabolite excreted by the kidney that produces oxidant stress and death of renal cells at pathophysiological concentrations. (elsevierpure.com)
  • These studies employed LLC-PK1 cells, a renal epithelial cell line from pig kidney and NRK-52E (NRK) cells, a line from normal rat kidney, and compared the effects of oxalate with those of known apoptotic agents. (elsevierpure.com)
  • These studies indicated that oxalate-induced death of renal epithelial cells exhibits several features characteristic of apoptotic cell death, including increased production of ceramide, increased abundance of apoptotic bodies, and marked sensitivity to the level of expression of the antiapoptotic gene bel-2. (elsevierpure.com)
  • Oxalate-induced cell death also exhibits several characteristics of necrotic cell death in that the majority of the cells exhibited cellular and nuclear swelling after oxalate treatment and showed little evidence of DNA cleavage by TUNEL assay. (elsevierpure.com)
  • These results suggest that toxic concentrations of oxalate trigger both forms of cell death in renal epithelial cells. (elsevierpure.com)
  • We found that "knockout" mice lacking one of these transport proteins have a high incidence of calcium oxalate urinary stones, the same type that is most common in human patients with kidney stones. (yale.edu)
  • We showed that the cause of the calcium oxalate kidney stones is a very high concentration of oxalate in the urine. (yale.edu)
  • We found that this kidney transport protein also plays a very crucial role in the intestine, where it secretes oxalate and thereby limits how much of ingested oxalate is absorbed and then excreted in the urine. (yale.edu)
  • We have also been studying mechanisms by which oxalate crystals induce inflammation and thereby cause damage to the kidney and other tissues. (yale.edu)
  • For example, work with mice lacking anion exchanger Slc26a6, which can function as an oxalate transporter, revealed a phenotype of calcium oxalate kidney stones. (yale.edu)
  • This finding in turn has motivated studies on the mechanisms and regulation of oxalate transporters and their roles in oxalate homeostasis, urolithiasis, and crystal-induced inflammation in the kidney and other tissues. (yale.edu)
  • In various mouse and rat models of obesity and type 1 and 2 diabetes mellitus, eCBs generated in various renal cells activate CB 1 receptors and contribute to the development of oxidative stress, inflammation, and renal fibrosis. (degruyter.com)
  • The liver is a major site of metabolism of many endogenous compounds and xenobiotics since hepatocytes (which comprise 80% of the liver cells) contain large amounts of smooth endoplasmic reticulum, where many metabolizing enzymes reside. (aspetjournals.org)
  • Gill ventilation rates, general condition factors and mortalities were determined and liver, kidney, gut and gill were assessed histopathologically and immunhistologically. (uni-konstanz.de)
  • Histopathological alterations in liver, gastrointestinal tract and kidney, which were also immunopositive for microcystin suggested causality of tissue damage and the presence of microcystins. (uni-konstanz.de)
  • When used improperly, it accumulates in the bodies of animals, and its main targets are the kidney and ear tissues [2]. (biomedres.us)
  • One approach involves the generation of isoform- and phospho-specific polyclonal and monoclonal antibodies to identify the cellular and subcellular sites of expression of ion exchangers in the kidney and other tissues, and to study their regulation. (yale.edu)
  • Here, we identified an inducible chemokine interleukin-8 (CXCL8/IL-8), which could promote coronavirus porcine epidemic diarrhea virus (PEDV) infection in African green monkey kidney epithelial cells (Vero) and Lilly Laboratories cell-porcine kidney 1 epithelial cells (LLC-PK1). (bvsalud.org)
  • Epithelial cell line originally derived from porcine kidneys. (bvsalud.org)
  • AA had a comparable effect on the cell cycle in primary human and porcine cells and the rat NRK-52E cell line following 48 h exposure, also corroborated by the reduced 3H-thymidine incorporation in NRK-52E cells. (uni-konstanz.de)
  • In addition, DNA unwinding, suggestive of enhanced DNA damage, was observed in primary porcine cells. (uni-konstanz.de)
  • Abbexa Laboratories manufactures the abbexa llc reagents distributed by Genprice. (celulastroncales.org)
  • Goblet Cells" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (childrensmercy.org)
  • Goblet cells secrete MUCUS. (childrensmercy.org)
  • This graph shows the total number of publications written about "Goblet Cells" by people in this website by year, and whether "Goblet Cells" was a major or minor topic of these publications. (childrensmercy.org)
  • Below are the most recent publications written about "Goblet Cells" by people in Profiles. (childrensmercy.org)
  • Decynium-22 is a cationic derivative of quinoline, and a potent inhibitor of the plasma membrane monoamine transporter (PMAT), as well as all members of the organic cation transporter (OCT) family in both human and rat cells. (wikipedia.org)
  • Using the transporter profiling approach, Dr. McDonough and her colleagues have defined how stimuli such as dietary salts, Ang II, kidney injury, and immune cytokines increase renal sodium transport in a region specific manner along the nephron and how resultant hypertension provokes intrarenal responses to decrease sodium reabsorption (via pressure natriuresis), a response that defines the blood pressure set point in the kidney. (usc.edu)
  • The uptake of [ 3 H]vinblastine, a substrate of P-gp, by Caco-2 cells was measured. (aspetjournals.org)
  • No significant effect on the uptake of 3- O -[ 3 H]methylglucose or [ 14 C]phenylalanine by Caco-2 cells was found, compared with the control. (aspetjournals.org)
  • The ethyl acetate extract of orange juice and these methoxyflavones also increased steady-state [ 3 H]vinblastine uptake by LLC-GA5-COL300 cells (a cell line transfected with human MDR1 cDNA). (aspetjournals.org)
  • Even when released, they remain largely membrane associated due to their hydrophobic nature and can be taken up by cells via a high-affinity uptake mechanism [ 23 ], which is followed by their enzymatic degradation. (degruyter.com)
  • She has delivered the 2010 Suk Ki Hong Memorial Lecture at SUNY Buffalo, the 2015 Rudy Clarenberg Distinguished Lecture at Kansas State University, the J.J. Smith Memorial Lecture at Medical College of Wisconsin, the keynote lecture at the Annual Young Investigator Symposium during American Society of Nephrology (ASN) Kidney Week (2016) and the Ulrich Hopfer Lectureship, Case Western Reserve University School of Medicine. (usc.edu)
  • As mTOR induces anabolism and inhibits autophagy, and TFEB is a master transcription factor for lysosomal enzymes, it is possible that this pathway plays a role in the inhibition of lysosomal enzymes in proximal tubule cells. (unifesp.br)
  • Inhibition by berberine of cyclooxygenase-2 transcriptional activity in human colon cancer cells. (ppt-health.com)
  • In order to prevent oxidative stress, cells were pre-treated with three different concentrations of l-arginine and vitamin E. Oxidative stress was evaluated by determining the expression of superoxide dismutase (SOD), osteopontin (OPN), and by the concentration of glutathione (GSH). (kbco.hr)
  • EDa protects cells by eliminating ROS from multiple types of cells and by reducing oxidative stress [8-10]. (biomedres.us)
  • The levels of nitric oxide (NO) was increased by AGE-BSA in all cell lines, suggesting oxidative stress, and Western blotting has shown that, among the investigated proteins, cathepsin B, mTOR and transcription factor EB (TFEB) were the most significantly affected by exposure to AGE-BSA. (unifesp.br)
  • Chronic exposure to cisplatin, a potent anticancer drug, causes irreversible kidney damage. (mdpi.com)
  • Exposure to HG had no effect on the cathepsin B activity, but AGE-BSA caused a marked decrease in LLC-PK1, and increased the enzyme activities in the other cell lines. (unifesp.br)
  • Effects of 24 and 48 h AA exposure on cell number and MTT reduction were studied. (uni-konstanz.de)
  • Methods and Results We demonstrate that knockdown of the α-1 isoform of Na/K-ATPase causes a reduction in CTS-induced macrophage infiltration in renal tissue as well reduces the accumulation of immune cells in the peritoneal cavity in vivo. (mcw.edu)
  • We used doubly perfused bullfrog kidneys in vivo , and switched the basolateral solution (renal portal vein) to experimental solutions. (cellphysiolbiochem.com)
  • The antiproliferative activity of (H2ind)[OsIVCl5(2H-ind)] (1) was found to be superior to that of (H2ind)[OsIVCl5(1H-ind)] (2) in one of three human cancer cell lines applied but inferior in the in vivo xeno-transplantation model. (erksignal.com)
  • Acid secretion by parietal cells: relative roles for [Ca2+]i and protein kinase C. Gastroenterology. (uchicago.edu)
  • Salva A, Hardy MP, Wu XF, Sottas CM, MacLaughlin DT, Donahoe PK, Lee MM. M?llerian-inhibiting substance inhibits rat Leydig cell regeneration after ethylene dimethanesulphonate ablation. (umassmed.edu)
  • The existence of specific receptors for plant-derived molecules in mammalian cells initiated a search for specific endogenous ligands. (degruyter.com)
  • My clinical interests are acute kidney injury, proteinuric kidney diseases, hypertension, electrolyte abnormalities and management of various chronic kidney diseases and dialysis. (massgeneral.org)
  • Background Recent studies have highlighted a critical role for a group of natriuretic hormones, cardiotonic steroid (CTS), in mediating renal inflammation and fibrosis associated with volume expanded settings, such as chronic kidney disease. (mcw.edu)
  • However, the association between betel-nut chewing and risk for chronic kidney disease (CKD) is unknown. (cambridge.org)
  • It is present in both the central nervous system and peripheral organs including the kidney. (degruyter.com)
  • They are scattered in the epithelial linings of many organs, especially the SMALL INTESTINE and the RESPIRATORY TRACT. (childrensmercy.org)
  • Building on these findings, the lab addressed the coordinated and concerted control of extracellular potassium by skeletal muscle, kidney and gut in response to altered metabolic and electrolyte demands and have begun to use these findings to address the mechanisms responsible for the cardiovascular benefit of raising dietary K/Na ratio. (usc.edu)
  • Positive correlation of SOD and OPN expression as well as GSH concentration was observed in all groups of cells. (kbco.hr)
  • Concerning lysosomal enzymes, the main cysteine-protease expressed by these cells was cathepsin B, and its concentration was much higher in epithelial than in mesangial cells. (unifesp.br)
  • Temperature, oxygen concentration, pH-value, P. rubescens cell density and microcystin concentration were recorded and the fish were observed for behavioural changes and examined for parasite infestations. (uni-konstanz.de)
  • Unlike classical neurotransmitters, eCBs are not stored in vesicles, and the mechanism of their release from cells is not yet clear. (degruyter.com)
  • The nature of the response has important consequences for the injured tissue in that necrotic cells elicit inflammatory responses, whereas apoptotic cells do not. (elsevierpure.com)
  • In this study, the effects of GM and EDa on the apoptotic rate of cells and the activity of Superoxide Dismutase (SOD), Glutathione Peroxidase (GSH-Px), Catalase (CAT), and Malondialdehyde (MDA) were examined. (biomedres.us)
  • The cells were maintained in DMEM high glucose media supplemented with 10% FBS along with the 1% antibiotic-antimycotic solution in the atmosphere of 5% CO 2 , 18 to 20% O 2 at 37°C temperature in the CO 2 incubator and sub cultured for every 2 days. (clinicsinoncology.com)
  • Impact of high glucose and AGEs on cultured kidney-derived cells. (unifesp.br)
  • The effects of high glucose (HG) and advanced glycation end products (AGEs) on cell viability, lysosomal enzymes and other effectors of cell signaling of cultured kidney cells were studied. (unifesp.br)
  • Steroid-producing cells in the interstitial tissue of the TESTIS. (umassmed.edu)
  • Pgp antagonism was defined by interactions of the drugs with four cell lines (LLC-PK1, L-MDR1, L-mdr1a, and L-mdr1b) using a microfluorometric calcein-AM assay and characterized for their inhibitor constant (K(i)) toward calcein-AM. The compounds were further defined for their ability to inhibit MDR1 by their effect on vinblastine accumulation into L-MDR1 cells. (nih.gov)
  • Chemokine production by epithelial cells is crucial for neutrophil recruitment to sites of inflammation during viral infection. (bvsalud.org)
  • When responding to viral infection, the CD4 + T cells mainly have a Th1-type phenotype expressing T-bet and produce large amounts of IFNγ [ 12 ]. (biomedcentral.com)
  • Depending on the cell density, exposed fish showed behavioural changes, including increased ventilation rates possibly representing a physiological stress response. (uni-konstanz.de)
  • The kidney is an important excretory organ of the body, and tubule cells are the basic structural and functional unit that constitutes the kidney. (biomedres.us)
  • None of the described functional or morphologic alterations to the kidney are pathognomonic to ADR. (pharmacy180.com)
  • We used cell lines expressing human, mouse, and rat mdr1 genes. (nih.gov)
  • The Abbexa Llc reagent is RUO (Research Use Only) to test human serum or cell culture lab samples. (celulastroncales.org)
  • Several permanent and immortalised cell lines of human and non-human origin are available, offer-ing several advantages over primary cultures such as an unlimited life span and the lack of time-consuming isolation procedures. (pharmacy180.com)
  • Initially, her lab focused on regulation of the biosynthesis and assembly of sodium pump subunits and molecular mechanisms controlling sodium pump isoforms in kidney, cardiac and skeletal muscle by potassium, hormones, and other factors under normal conditions and during human heart failure. (usc.edu)
  • Dr. McDonough is a recipient of multiple Awards including an American Heart Association (AHA) Established Investigator Award (1984), the 2009 Starling Distinguished Lectureship from the American Physiological Society (APS), the 2014 Donald Seldin Lectureship from the AHA Council on the Kidney in Cardiovascular Disease (KCVD), the Robert Schrier Lecture at ASN Kidney week (2017), and the Steven Hebert (APS, 2018). (usc.edu)
  • These projects have engaged the McDonough lab with expert collaborators across the USA and around the world and attracted attention to the important role of the kidney in balancing the intertwined homeostasis of sodium, potassium and blood pressure. (usc.edu)
  • Our general goal is to characterize the mechanisms regulating sodium, acid-base, and anion excretion by the kidney. (yale.edu)
  • Epithelial and Endothelial Adhesion of Immune Cells Is Enhanced by Cardiotonic Steroid Signaling Through Na+/K+-ATPase-α-1. (mcw.edu)
  • Herein, we tested the hypothesis that CTS signaling through Na + /K + -ATPase α-1 (NKA α-1) enhances immune cell recruitment and adhesion to renal epithelium that ultimately advance renal inflammation. (mcw.edu)
  • The current review focuses on the role of the eCB system in normal kidney function and various diseases, such as diabetes and obesity, that directly contributes to the development of renal pathologies. (degruyter.com)